| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,168 |
| تعداد مقالات | 35,197 |
| تعداد مشاهده مقاله | 50,932,587 |
| تعداد دریافت فایل اصل مقاله | 90,938,130 |
Functional characterization of endophytic fungi from Tamarix ramosissima | ||
| Mycologia Iranica | ||
| دوره 12، شماره 1، شهریور 2025، صفحه 83-93 اصل مقاله (864.62 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2025.369742.1314 | ||
| نویسندگان | ||
| Mostafa Ebadi* 1؛ Solmaz Najari1؛ Leila Zarandi Miandoab1؛ Hamideh Abdolrahmani1؛ Nader Chaparzadeh1؛ Ali Ebadi2 | ||
| 1Department of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| 2Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran | ||
| چکیده | ||
| Endophytic fungi are commonly found in the root endosphere and can enhance plant growth through various mechanisms. This study aimed to isolate cultivable endophytic fungi associated with the roots of Tamarix ramosissima and evaluate their plant growth-promoting properties. Thirty-five fungal endophytes belonging to the phylum Ascomycota, representing four different genera, were obtained from the endosphere of T. ramosissima: Alternaria, Aspergillus, Fusarium, and Talaromyces. These fungal endophytes showed different abilities to solubilize phosphate and produce indole-3-acetic acid (IAA). The isolates of T. allahabadensis (T3) and A. niger (T4) showed different efficiencies in phosphate solubilization. Almost all fungal isolates produced IAA, with the highest concentration (0.699 µg/ml) found in the isolate of F. solani (T11). Among the tested endophytes, only A. fumigatus (T15) significantly increased wheat root length (75% increase vs control, p<0.05). The extensive root system of T. ramosissima may be due to symbiosis with IAA-producing endophytic fungi, which enhance root development and water uptake in dry conditions. Additionally, these fungi can increase soil phosphorus levels, further promoting plant growth. | ||
| کلیدواژهها | ||
| Ascomycota؛ Aspergillus fumigatus؛ Endosphere؛ IAA؛ Root developing | ||
| مراجع | ||
|
Adnan M, Alshammari E, Ashraf SA, Patel K, Lad K, Patel M. 2018. Physiological and Molecular Characterization of Biosurfactant Producing Endophytic Fungi Xylaria regalis from the Cones of Thuja plicata as a Potent Plant Growth Promoter with Its Potential Application. BioMed Research International. 2018(1): 7362148. https://doi.org/10.1155/2018/7362148.
Anyasi R, Atagana HI. 2019. Endophyte: Understanding the microbes and its applications. Pakistan Journal of Biological Sciences. 22: 154-167. https://doi.org/10.3923/pjbs.2019.154.167.
Begerow D, Nilsson H, Unterseher M, Maier W. 2010. Current state and perspectives of fungal DNA barcoding and rapid identification procedures. Applied Microbiology and Biotechnology. 87: 99-108. https://doi.org/10.1007/S00253-010-2585-4/METRICS.
Bilal L, Asaf S, Hamayun M, Gul H, Iqbal A, Ullah I, Lee IJ, Hussain A. 2018. Plant growth promoting endophytic fungi Asprgillus fumigatus TS1 and Fusarium proliferatum BRL1 produce gibberellins and regulates plant endogenous hormones. Symbiosis. 76: 117-127. https://doi.org/10.1007/S13199-018-0545-4/METRICS.
Bononi L, Chiaramonte JB, Pansa CC, Moitinho MA, Melo IS. 2020. Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth. Scientific Reports. 10: 1-13. https://doi.org/10.1038/s41598-020-59793-8.
de Carvalho JO, Broll V, Martinelli AHS, Lopes FC. 2020. Endophytic fungi: Positive association with plants. In Molecular Aspects of Plant Beneficial Microbes in Agriculture. (Sharma V, Salwan R, Al-Ani LKT, Eds): 321–332. Academic Press, Cambridge, MA, USA. https://doi.org/10.1016/B978-0-12-818469-1.00026-2. Delgado M, Mendez J, Rodríguez-Herrera R, Aguilar CN, Cruz-Hernández M, Balagurusamy N. 2014. Characterization of phosphate-solubilizing bacteria isolated from the arid soils of a semi-desert region of north-east Mexico. Biological Agriculture and Horticulture. 30: 211-217. https://doi.org/10.1080/01448765.2014.909742.
Di Tomaso JM. 1998. Impact, Biology, and Ecology of Saltcedar (Tamarix spp.) in the Southwestern United States. Weed Technology. 12: 326-336. https://doi.org/10.1017/S0890037X00043906.
Doilom M, Guo JW, Phookamsak R, Mortimer PE, Karunarathna SC, … et al. 2020. Screening of Phosphate-Solubilizing Fungi From Air and Soil in Yunnan, China: Four Novel Species in Aspergillus, Gongronella, Penicillium, and Talaromyces. Frontiers in Microbiology. 11: 1-24. https://doi.org/10.3389/fmicb.2020.585215.
Elias F, Woyessa D, Muleta D. 2016. Phosphate Solubilization Potential of Rhizosphere Fungi Isolated from Plants in Jimma Zone, Southwest Ethiopia. International Journal of Microbiology. 2016(1): 5472601. https://doi.org/10.1155/2016/5472601.
Fan S, Wang Q, Dai J, Jiang J, Hu X, Subbarao KV. 2021. The Whole Genome Sequence of Fusarium redolens Strain YP04, a Pathogen that Causes Root Rot of American Ginseng. Phytopathology. 111: 2130-2134. https://doi.org/10.1094/PHYTO-03-21-0084-A/ASSET/IMAGES/LARGE/PHYTO-03-21-0084-AT2.JPEG.
Fu SF, Wei JY, Chen HW, Liu YY, Lu HY, Chou JY. 2015. Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms. Plant Signaling & Behavior. 10(8): 1048052. https://doi.org/10.1080/15592324.2015.1048052.
Gordon SA, Weber RP. 1951. Colorimetric estimation of indoleacetic acid. Plant Physiology. 26(1): 192. https://doi.org/10.1104/PP.26.1.192.
Imade YN, Rukayat OK, Olubunmi TA, Busayo TA. 2020. Isolation of an emerging thermotolerant medically important Fungus, Lichtheimia ramosa from soil. African Journal of Microbiology Research. 14: 237-241. https://doi.org/10.5897/ajmr2020.9358.
Jagadish R, Chowdappa S. 2021. Phytochemical Analysis, Extracellular Enzymes and Antioxidant Activity of Endophytic Fungi from Cymbopogon citratus L. International Journal of Botany Studies. 6: 509-516.
Jain R, Saxena J, Sharma V. 2017. The ability of two fungi to dissolve hardly soluble phosphates in solution. Mycology. 8: 104-110. https://doi.org/10.1080/21501203.2017.1314389.
Khalil AMA, Hassan SED, Alsharif SM, Eid AM, Ewais EED, … et al. 2021. Isolation and Characterization of Fungal Endophytes Isolated from Medicinal Plant Ephedra pachyclada as Plant Growth-Promoting. Biomolecules. 11(2): 140. https://doi.org/10.3390/BIOM11020140.
Kuhad RC, Singh S, Lata, Singh A. 2011. Phosphate-Solubilizing Microorganisms. In Bioaugmentation, Biostimulation and Biocontrol. (Singh A, Parmar, N, Kuhad R, Eds): 65-84. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19769-7_4Lacap D, Hyde K, Liew E. 2003. An evaluation of the fungal’morphotype’concept based on ribosomal DNA sequences. Fungal Diversity. 12: 66.
Lynn TM, Win HS, Kyaw EP, Latt ZK, San San Yu, 2013. Characterization of Phosphate Solubilizing and Potassium Decomposing Strains and Study on their Effects on Tomato Cultivation. International Journal of Innovation and Applied Studies. 3: 959-966.
Meents AK, Furch ACU, Almeida-Trapp M, Özyürek S, Scholz SS, … et al. 2019. Beneficial and pathogenic Arabidopsis root-interacting fungi differently affect auxin levels and responsive genes during early infection. Frontiers in Microbiology. 10: 1-14. https://doi.org/10.3389/fmicb.2019.00380.
Mehmood A, Hussain A, Irshad M, Khan N, Hamayun M, … et al. 2018. IAA and flavonoids modulates the association between maize roots and phytostimulant endophytic Aspergillus fumigatus greenish. Journal of Plant Interaction. 13: 532-542. https://doi.org/10.1080/17429145.2018.1542041.
Mursyida E, Mubarik N, Tjahjoleksono A. 2015. Selection and identification of phosphate-potassium solubilizing bacteria from the area around the limestone mining in Cirebon quarry. Research Journal of Microbiology. 10: 270-279.
Naziya B, Murali M, Amruthesh KN. 2019. Plant Growth-Promoting Fungi (PGPF) Instigate Plant Growth and Induce Disease Resistance in Capsicum annuum L. upon Infection with Colletotrichum capsici (Syd.) Butler & Bisby. Biomolecules. 10(1): 41. https://doi.org/10.3390/BIOM10010041.
Nesme T, Metson GS, Bennett EM, 2018. Global phosphorus flows through agricultural trade. Global Environmental Change. 50: 133-141. https://doi.org/10.1016/J.GLOENVCHA.2018.04.004.
Nira ST, Hossain MF, Hassan NUM, Islam T, Akanda AM. 2022. Alternaria leaf spot of broccoli caused by Alternaria alternata in Bangladesh. Plant Protection Science. 58: 49-56. https://doi.org/10.17221/44/2020-PPS.
Perrone G, Gallo A. 2017. Aspergillus species and their associated mycotoxins. Methods in Molecular Biology. 1542: 33-49. https://doi.org/10.1007/978-1-4939-6707-0_3/COVER.
Prasad MR, Sagar BV, Devi GU, Triveni S, Rao SRK, … et al. 2017. Isolation and Screening of Bacterial and Fungal Isolates for Plant Growth Promoting Properties from Tomato (Lycopersicon esculentum Mill.). International Journal of Current Microbiology and Applied Sciences. 6: 753-761. https://doi.org/10.20546/ijcmas.2017.608.096.
Rahnama K, Jahanshahi M, Nasrollanejad S, Fatemi MH, Shahiri Tabarestani M. 2016. Identification of Volatile Organic Compounds from Trichoderma virens (6011) by GC-MS and Separation of a Bioactive Compound via Nanotechnology. International Journal of Engineering. 29: 1347-1353. https://doi.org/10.5829/idosi.ije.2016.29.10a.04.
Raja HA, Miller AN, Pearce CJ, Oberlies NH. 2017. Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. Journal of Natural Products 80: 756-770.https://doi.org/10.1021/ACS.JNATPROD.6B01085/ASSET/IMAGES/LARGE/NP-2016-01085V_0007.JPEG.
Selvi K, Paul J, Vijaya V, Saraswathi K. 2016. Analyzing the Efficacy of Phosphate Solubilizing Microorganisms by Enrichment Culture Techniques. Biochemistry & Molecular Biology Journal. 3(1): 1-7. https://doi.org/10.21767/2471-8084.100027.
Show PL, Oladele KO, Siew QY, Aziz Zakry FA, Lan JCW, … et al. 2015. Overview of citric acid production from Aspergillus niger. Front Life Science. 8: 271-283. https://doi.org/10.1080/21553769.2015.1033653.
Sperber JI. 1958. The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural Research. 9(6): 778-781. https://doi.org/10.1071/AR9580778.
Strobel G, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology
and Molecular Biology Reviews. 67(4): 491-502. https://doi.org/10.1128/MMBR.67.4.491-502.2003.
Suebrasri T, Harada H, Jogloy S, Ekprasert J, Boonlue S. 2020. Auxin-producing fungal endophytes promote growth of sunchoke. Rhizosphere. 16: 100271. https://doi.org/10.1016/J.RHISPH.2020.100271.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution. 30(12): 2725-2729. https://doi.org/10.1093/molbev/mst197
Tian J, Ge F, Zhang D, Deng S, Liu X. 2021. Roles of Phosphate Solubilizing Microorganisms from Managing Soil Phosphorus Deficiency to Mediating Biogeochemical P Cycle. Biology. 10(2) 158. https://doi.org/10.3390/BIOLOGY10020158.
Toju H, Tanabe AS, Yamamoto S, Sato H. 2012. High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples. PLoS One. 7: e40863. https://doi.org/10.1371/JOURNAL.PONE.0040863.
Vardasbi H, Saremi H, Fotouhifar KB, Kaveh H, Javan-Nikkhah M. 2020. Novel endophytic species of Talaromyces sect. Talaromyces associated with saffron plant to the mycobiota of Iran. Mycolgia Iranica. 7: 219-229. https://doi.org/10.22043/MI.2021.124383.
Varga T, Hixson KK, Ahkami AH, Sher AW, Barnes ME, … et al. 2020. Endophyte-Promoted Phosphorus Solubilization in Populus. Frontiers in Plant Science. 11: 567918. https://doi.org/10.3389/fpls.2020.567918.
Wang X, Wang C, Sui J, Liu Z, Li Q, … et al. 2018. Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities. AMB Express. 8: 1-12. https://doi.org/10.1186/S13568-018-0593-4/FIGURES/6.
Yilmaz N, Visagie CM, Houbraken J, Frisvad JC, Samson RA. 2014. Polyphasic taxonomy of the genus Talaromyces. Studies in Mycology 78: 175-341. https://doi.org/10.1016/J.SIMYCO.2014.08.001.
Zhu H, Qu F, Zhu LH. 1993. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Research. 21: 5279. https://doi.org/10.1093/NAR/21.22.5279.
| ||
|
آمار تعداد مشاهده مقاله: 460 تعداد دریافت فایل اصل مقاله: 278 |
||