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صفری سنجانی, علی اکبر, کرمی, لیلا. (1404). پیامد کاربرد خاکستر زغال سنگ بر شناسه‌های همزیستی گیاه نخود سپید مایه‌زنی شده با مزوریزوبیوم و قارچ‌های میکوریز. سامانه مدیریت نشریات علمی, 13(1), 67-89. doi: 10.22092/sbj.2025.368447.278
علی اکبر صفری سنجانی; لیلا کرمی. "پیامد کاربرد خاکستر زغال سنگ بر شناسه‌های همزیستی گیاه نخود سپید مایه‌زنی شده با مزوریزوبیوم و قارچ‌های میکوریز". سامانه مدیریت نشریات علمی, 13, 1, 1404, 67-89. doi: 10.22092/sbj.2025.368447.278
صفری سنجانی, علی اکبر, کرمی, لیلا. (1404). 'پیامد کاربرد خاکستر زغال سنگ بر شناسه‌های همزیستی گیاه نخود سپید مایه‌زنی شده با مزوریزوبیوم و قارچ‌های میکوریز', سامانه مدیریت نشریات علمی, 13(1), pp. 67-89. doi: 10.22092/sbj.2025.368447.278
صفری سنجانی, علی اکبر, کرمی, لیلا. پیامد کاربرد خاکستر زغال سنگ بر شناسه‌های همزیستی گیاه نخود سپید مایه‌زنی شده با مزوریزوبیوم و قارچ‌های میکوریز. سامانه مدیریت نشریات علمی, 1404; 13(1): 67-89. doi: 10.22092/sbj.2025.368447.278

پیامد کاربرد خاکستر زغال سنگ بر شناسه‌های همزیستی گیاه نخود سپید مایه‌زنی شده با مزوریزوبیوم و قارچ‌های میکوریز

زیست شناسی خاک
مقاله 5، دوره 13، شماره 1، شهریور 1404، صفحه 67-89    اصل مقاله (1.08 M)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22092/sbj.2025.368447.278
نویسندگان
علی اکبر صفری سنجانی* ؛ لیلا کرمی
گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
چکیده
برای بررسی پیامد کاربرد خاکستر زغال‌سنگ گونه بیتومینه بر همزیستی سه گانه گیاه نخود-قارچ-باکتری، آزمایشی گلدانی بر یک خاک سترون شده به گونه فاکتوریل با طرح کاملا تصادفی در سه تکرار انجام شد. فاکتورهای آزمایش شده کاربرد خاکستر زغال‌سنگ در 4 اندازه (0، 5/2 ، 5 و 10 درسد) ، مایه‌زنی قارچ میکوریزا در سه تیمار (بدون مایه‌زنی، مایه‌زنی با قارچ Funneliformis mosseae و مایه‌زنی با قارچ Rhizophagus irregularis) و مایه‌زنی Mesorhizobium ciceri در دو تیمار (بدون مایه‌زنی و مایه‌زنی با مزوریزوبیوم) بود. تیمار خاک با 5/2 درسد خاکستر زغال‌سنگ مایه افزایش چشم‌گیر شناسه‌های همزیستی گیاه نخود با قارچ‌های میکوریزی و Mesorhizobium ciceri در برابر گواه آزمایش شد، ولی این شناسه‌ها در تیمارهای کاربرد 5 و 10 درسد خاکستر در برابر گواه کاهش پیدا کردند. میانگین شمار گره‌های ریزوبیومی در تیمار 5/2 درسد خاکستر زغال‌سنگ در گلدان مایه‌زنی شده با Funneliformis mosseae بالاترین (66/41) و در تیمار 10 درسد خاکستر زغال‌سنگ و بدون مایه‌زنی میکوریزا کمترین (66/3) بود. کاربرد کودهای زیستی میکوریزی و مزوریزوبیوم هر یک مایه بهبود همزیستی گیاه با دیگری شد، ولی این پیامدهای سودمند آنها بر هم، در تیمار 10 درسد خاکستر زغال‌سنگ چشم‌گیر نبود. اگر چه شناسه‌های بررسی شده در میان قارچ‌ها ناهمانندی چندانی نداشت ولی بردباری Funneliformis mosseae در برابر خاکستر زغال‌سنگ کمی بیشتر Rhizophagus irregularis بود. این پژوهش نشان داد که کاربرد خاکستر زغال‌سنگ گونه بیتومینه در اندازه کم (5/2 درسد) از راه بهبود همزیستی گیاه نخود با قارچ‌های میکوریزی و Mesorhizobium ciceri می‌تواند در افزایش رشد و کارکرد آن سودمند باشد ولی کاربرد آن در اندازه‌های فراوان (5 و 10 درسد) در خاک می‌تواند ویژگی‌های خاک را برای همزیستی گیاه نخود ناشایست کند.
کلیدواژه‌ها
زغال‌سنگ بیتومینه؛ نخود ایرانی؛ Funneliformis mosseae؛ Mesorhizobium ciceri؛ Rhizophagus irregularis
عنوان مقاله [English]
The effect of the application of coal ash in soil on the symbiotic indices of white pea inoculated with Mesorhizobium and mycorrhizal fungi
نویسندگان [English]
َAli Akbar Safari Sinegani؛ Leila Karami
Department of Soil Sciences and Engineering, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
چکیده [English]
Background and Objectives: Iranian chickpea (Cicer arietinum L.) ranks third globally in edible legumes, but leads in the Mediterranean and South Asia. It enhances soil nitrogen through symbiosis with Rhizobium bacteria, contributing to sustainable agriculture. Coal, a major energy source, produces ash that can pollute if misused but can improve soil properties when applied properly. Coal ash enhances plant growth and nutrient uptake but higher concentrations may reduce beneficial microbes. Mycorrhizal fungi and Rhizobium bacteria form valuable symbiotic relationships with legumes. The study aimed to investigate the effects of bituminous coal ash on the tripartite symbiosis between chickpea plants, mycorrhizal fungi, and rhizobial bacteria.

Materials and Methods: The study was conducted in a low-phosphorus soil with a loam texture, collected from Azandarian, Hamadan Province, Iran. Bituminous coal ash was obtained from the Shek-Maidan mine, Kermanshah Province. Physical and chemical properties of the soil and coal ash were analyzed using standard methods. A factorial experiment in a completely randomized design with three replications was conducted on sterilized soil. The factors included coal ash at four levels (0%, 2.5%, 5%, and 10%), mycorrhizal fungi inoculation (Funneliformis mosseae and Rhizophagus irregularis, or no inoculation), and Mesorhizobium ciceri inoculation (with or without). Chickpea seeds were surface-sterilized and inoculated with Mesorhizobium before sowing. Mycorrhizal inoculum and chickpea seeds were obtained from certified sources. Soil and coal ash mixtures were sterilized in an autoclave, and 3 kg of the mixture was placed in each pot. Mycorrhizal inoculum (100 g) was applied below the seeds. Six chickpea seeds were planted per pot and thinned to two after germination. The plants were grown under greenhouse conditions without any additional fertilizers, and pots were watered regularly. At flowering, plants were harvested, and roots were separated from the soil. Nodules were counted, and root colonization by mycorrhizal fungi was assessed using staining techniques. Glomalin-related soil proteins were extracted and quantified, and fungal spores in the soil were counted using centrifugation in a sugar solution. Data were analyzed using Excel and SAS software.

Results: The results indicated that the application of coal ash at the 2.5% level significantly improved the symbiotic interactions between chickpea plants and both mycorrhizal fungi and rhizobial bacteria compared to the control (no coal ash). Specifically, this treatment increased the number of nodules formed by Mesorhizobium and enhanced the establishment of mycorrhizal symbiosis. In contrast, applying higher concentrations of coal ash (5% and 10%) resulted in a notable decline in these beneficial symbiotic relationships, as evidenced by a decrease in both mycorrhizal colonization and rhizobial nodule formation.

The highest mean number of rhizobial nodules per pot (41.66) was found in treatments where both Mesorhizobium inoculation and 2.5% coal ash application were combined, along with inoculation of Funneliformis mosseae. This combination produced the most favorable environment for both the mycorrhizal fungi and rhizobia. On the other hand, the lowest number of rhizobial nodules (3.66) was recorded in the 10% coal ash treatment where no mycorrhizal inoculation was applied, suggesting that excessive coal ash inhibits the formation of beneficial microbial interactions.

In terms of fungal performance, both Funneliformis mosseae and Rhizophagus irregularis improved the symbiosis between the chickpea plants and the rhizobial bacteria. However, the beneficial effects were not as significant in the 10% coal ash treatment, where the overall soil conditions seemed less conducive to the establishment of both mycorrhizal and rhizobial symbioses. Interestingly, among the two mycorrhizal fungi, Funneliformis mosseae showed slightly greater tolerance to the coal ash, maintaining better performance compared to Rhizophagus irregularis, especially at higher ash concentrations.

Conclusion: This study demonstrate that the application of bituminous coal ash in small amounts significantly enhances biological parameters, such as the abundance and weight of Mesorhizobium nodules on the roots of white chickpea plants, the percentage of mycorrhizal colonization of the roots, the number of Glomale spores in the soil, and soil glomalin or glycopeptides. Applying coal ash in low amounts (2.5%) could be beneficial for chickpea growth by improving symbiotic relationships with both mycorrhizal fungi and rhizobia, thus enhancing plant growth and function. However, increasing the coal ash concentration to higher levels (5% and 10%) can deteriorate the soil's ability to support these beneficial symbiotic interactions, leading to reduced plant growth. Although the response of the two fungi species was not significantly different, Funneliformis mosseae demonstrated greater tolerance and better performance in response to the harmful effects of ash. Inoculation with Mesorhizobium increased symbiotic associations with fungi, particularly Rhizophagus irregularis, in the plant, while the inoculation and application of mycorrhizal fungi promoted nodule formation and symbiosis between Mesorhizobium and the chickpea plant. Therefore, the application of 2.5% bituminous coal ash in the soil is beneficial for chickpea growth and its symbiotic relationship with microorganisms, whereas higher doses may have adverse effects on plant growth and overall soil health. This highlights the potential risks associated with high concentrations of coal ash in agricultural systems.
کلیدواژه‌ها [English]
Bitominous coal, Funneliformis mosseae, Iranian pea, Mesorhizobium ciceri, Rhizophagus irregularis
مراجع
  1. Ahmadzadeh, M., Sedaghati, E., Sabri-Riseh, R., Rahimi, A., MohammadiMirik, A.A. and Hatami, N. 2022. Effect of mycorrhizal fungi accompanied by some microorganisms and chemical compounds on growth and photosynthesis indices of corn. Soil Biology Journal, 10 (1), pp. 33-48.
  2. Ansari, M.S., Ahmad, G., Khan, A.A., Mohamed, H.I., 2023. Coal fly ash application as an eco-friendly approach for modulating the growth, yield, and biochemical constituents of Withania somnifera L. plants. Environment Science and Pollution Research International. 30(37), pp. 87958-87980. https://doi.org/10.1007/s11356-023-28318-x.
  3. Bedini, S., Avio, L. and Sbrana, C., 2013. Mycorrhizal activity and diversity in a long-term organic Mediterranean agroecosystem. Biology and Fertility of Soils, 49(6), pp.781–790. https://doi.org/1007/s00374-012-0770-6.
  4. Begum, N., Xiao, Y., Wang, L., Li, D., Irshad, A., Zhao, T., 2023. Arbuscular mycorrhizal fungus Rhizophagus irregularisalleviates drought stress in soybean with overexpressing the GmSPL9d gene by promoting photosynthetic apparatus and regulating the antioxidant system. Microbiological Research, 273, 127398. https://doi.org/10.1016/j.micres.2023.127398.
  5. Bower, C.A., Reitmeir, R.F. and Fireman, M., 1952. Exchangeable cation analysis of saline and alkali soils. Soil Science, 73(4), pp.251–261. https://doi.org/10.1097/00010694-195204000-00001.
  6. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), pp.248–254. https://doi.org/10.1016/0003-2697(76)90527-3.
  7. Budi, S.W. and Christina, F., 2013. The effects of coal waste powder amendment and arbuscular mycorrhizal fungi (AMF) on the growth of jabon (Anthocephalus cadamba Miq) seedling in Ultisol soil medium. Journal of Tropical Soil, 18(1), pp.59–66. https://doi.org/10.5400/jts.2013.18.1.59.
  8. Channabasava, A., Lakshman, H.C. and Muthukumar, T., 2015. Fly ash mycorrhizoremediation through Paspalum scrobiculatum, inoculated with Rhizophagus fasciculatus. Comptes Rendus Biologies, 338(1), pp.29–39. https://doi.org/10.1016/j.crvi.2014.11.002.
  9. Cheung, K.C., Wong, J.P.K., Zhang, Z.Q., Wong, J.W.C. and Wong, M.H., 2000. Revegetation of lagoon ash using the legume species Acacia auriculiformis and Leucaena leucocephala. Environmental Pollution, 109(1), pp.75–82. https://doi.org/10.1016/S0269-7491(99)00235-3.
  10. Daniels, B.A. and Skipper, H.D., 1982. Methods and principles of mycorrhizal research. American Phytopathological Society, St Paul, Minnesota.
  11. Ganjeali, A., Parsa, M. and Sabaghpour, S., 2008. Farming and agrosystems of pulses in pulses. JDM Press Iran.
  12. Garampalli, R.H., Deene, S. and Narayana Reddy, C., 2005. Infectivity and efficacy of Glomus aggregatum and growth response of Cajanus cajan (L.) Millsp in fly ash amended sterile soil. Environmental Biology of Soils, 26(5), pp.705–708. PMID: 16459561.
  13. Gebremariam, M., and Tesfay, T., 2021. Effect of P Application Rate and Rhizobium Inoculation on Nodulation, Growth, and Yield Performance of Chickpea (Cicer arietinum L.). International Journal of Agronomy, 2021, 8845489, https://doi.org/10.1155/2021/8845489.
  14. Gee, G.W. and Bauder, J.W., 1986. Method of soil analysis part 1: Physical and mineralogical methods. Soil Science Society of America, Madison, Wisconsin, USA.
  15. Geneva, M., Zehirov, G., Djonova, E., Kaloyanova, N., Georgiev, G. and Stancheva, I., 2006. The effect of inoculation of pea plants with mycorrhizal fungi and rhizobium on nitrogen and phosphorus assimilation. Plant Soil and Environment, 52(10), pp.435–440. https://doi.org/10.17221/3463-PSE.
  16. Giovannetti, M. and Mosse, B., 1980. An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytologist, 84(3), pp.489–500. https://doi.org/10.1111/j.1469-8137.1980.tb04556.x
  17. Heydarizadeh, M., Naghavi, H. and Moghaadam, M.R., 2013. Study of the chemical, physical characteristics and reject coal fertility to use in the agriculture activities (case study: Coal washing plant Zarand-Kerman). Water and Irrigation Engineering, 4(4), pp.58–71.
  18. Kochaki, A. and Banayan-Aval, M., 1993. Beans. Mashhad University Jahad Press, Iran.
  19. Loeppert, R.H. and Suarez, D.L., 1996. Methods of soil analysis part 3: Chemical methods. Soil Science Society of America, Madison, Wisconsin, USA.
  20. Mittra, B.N., Karmakar, S., Swain, D.K. and Ghosh, B.C., 2005. Fly ash a potential source of soil amendment and a component of integrated plant nutrient supply system. Fuel, 84(4), pp.1447–1451. . https://doi.org/1016/j.fuel.2004.10.019.
  21. Morphy, J. and Riley, J.P., 1962. A modified single solution method for the determination of phosphate in natural water. Analytica Chimica Acta, 27, pp.31–36. https://doi.org/10.1016/S0003-2670(00)88444-5.
  22. Mukerji, K.G. and Chmola, B.P., 2003. Compendium of mycorrhiza research. A.P.H. Publisher, New Delhi.
  23. Nadian Ghomsheh, H., 2024. Phosphorus uptake and transport mechanism in symbiotic plants with arbuscular mycorrhizal fungi (Knowns and unknowns). Soil Biology Journal, 12 (2), pp. 155-190. https://doi.org/ 10.22092/sbj.2024.366288.267.
  24. Pandey, V.C. and Singh, N., 2010. Impact of fly ash incorporation in soil systems. Agriculture, Ecosystems and Environment, 136(1-2), pp.16-27. https://doi.org/10.1016/j.agee.2009.11.013.
  25. Phillips, J.M. and Hayman, D.S., 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55(1), pp.158-161. https://doi.org/10.1016/S0007-1536(70)80110-3.
  26. Pichtel, J.R., 1990. Microbial respiration in fly ash/sewage sludge amended soils. Environmental Pollution, 63(3), pp.225-237. https://doi.org/10.1016/0269-7491(90)90156-7.
  27. Polcyn, W., Paluch-Lubawa, E., Lehmann, T. and Mikuła, R., 2019. Arbuscular mycorrhiza in highly fertilized maize cultures alleviates short-term drought effects but does not improve fodder yield and quality. Frontiers in Plant Science, 10, p.1510. https://doi.org/10.3389/fpls.2019.00496.
  28. Pourmirzaei, Z., Lakzian, A., Ali Asgharzad, N., Dehnad, A.R.and Hallajnia, A., Effects of Streptomycets on colonization and growth of white clover inoculated with arbuscular mycorrhiza. Soil Biology Journal, 9 (2), pp. 155-171. https://doi.org/ 22092/SBJ.2021.352206.208.
  29. Qiu, L., Bi, Y., Jiang, B., Wang, Z., Zhang, Y. and Zhakypbek, Y., 2019. Arbuscular mycorrhizal fungi ameliorate the chemical properties and enzyme activities of rhizosphere soil in reclaimed mining subsidence in northwestern China. Journal of Arid Land, 11(1), pp.135-147.  https://doi.org/10.1007/s40333-018-0019-9.
  30. Rezaei Bisotoni, M., Ghorbani, J., Vahabzadeh, G., Hodjati, S.M., 2023. Physical and chemical characteristics of substrate material for plant growth on coal wastes in rangelands of Kiasar, Sari, Mazandaran province. Journal of Rangeland, 16(4), pp. 652-665. https://doi.org/20.1001.1.20080891.1401.16.4.1.9.
  31. Roades, J.D., 1990. Methods of soil analysis part 3: Chemical methods. Soil Science Society of America, Madison, Wisconsin, USA.
  32. Sabannavar, S.J. and Lakshman, H.C., 2009. Effect of rock phosphate solubilization using mycorrhizal fungi and phosphobacteria on two high yielding varieties of Sesamum indicum World Journal of Agricultural Science, 5(4), pp.470-479.
  33. Saberi, A., Vahabzadekebriya, Gh., Hojjati, S.M., and Mosavi, S.R., 2023. The effect of coal mining on the accumulation of Pb and Zn and their spatial distribution in the surface soil of Komarzd. Water and Soil Management and Modeling, 3(3), pp. 56-71. https://doi.org/10.22098/mmws.2022.11395.1128.
  34. Safapur, M., Ardakani, M.R., Rejali, F., Khaghani, S. and Teymuri, M., 2012. Effect of co-inoculation of mycorrhiza and rhizobium on common bean (Phaseolus vulgaris). New Finding in Agriculture, 6(1), pp.21-35.
  35. Safari Sinegani, A.A., 2013. Soil biology and biochemistry. 4th ed. Bu-Ali Sina University Press Center, Iran.
  36. Safari-Sinegani, A.A. and Elyasi-Yeganeh, M., 2017. The occurrence of arbuscular mycorrhizal fungi in soil and root of medicinal plants in Bu-Ali Sina garden in Hamadan, Iran. Biological Journal of Microorganism, 5(20), pp.43-59. https://doi.org/22108/BJM.2017.21145.
  37. Safari Sinegani, A.A., Sharifi, Z., Safari Sinegani, M. 2010. Methods in applied microbiology. Iran, Hamadan: Bu-Ali Sina University Press Center, Iran.
  38. Selvam, A. and Mahadevan, A., 2002. Distribution of mycorrhizas in an abandoned fly ash pond and mined sites of Neyveli Lignite Corporation Tamil Nadu India. Basic and Applied Ecology, 3(3), pp.277-284. https://doi.org/10.1078/1439-1791-00107.
  39. SFS-EN, 2000. The European standard SFS-EN 12879 characterization of sludges. Determination of loss on ignition of dry mass. Finnish Standards Association SFS, Finnish Environmental Institute. Helsinki, Finland.
  40. Shaher, H., Naushin, F., Hasan, M., and Bagyaraj, D.J., 2024. Synergistic impact of Rhizobium and arbuscular mycorrhizal fungi on lentil plant tolerance to heavy metal-rich fly ash amended soil. Discover Plants, 9(1),  https://doi.org/10.1007/s44372-024-00010-5
  41. Singh, S.N., Kulshreshtha, K. and Ahmad, K.J., 1997. Impacts of fly-ash soil amendment on seed germination, seedling growth and metal composition of Vicia faba. Ecological Engineering, 9(3), pp.203-208. https://doi.org/10.1016/S0925-8574(97)10004-0.
  42. Skaggs, T.H., Arya, L.M., Shouse, P.J. and Mohanty, B.P., 2001. Estimating particle-size distribution from limited soil texture data. Soil Science Society of America Journal, 65(4), pp.1038-1044. https://doi.org/10.2136/sssaj2001.6541038x.
  43. Smith, S.E. and Read, D.J., 2008. Mycorrhizal symbiosis. 3rd ed. Academic Press, London, UK.
  44. Thomas, G.W., 1996. Methods of soil analysis part 3: Chemical methods. American Society of Agronomy and Soil Science Society of America, Madison, Wisconsin, USA.
  45. Vajpayee, P., Rai, U.N., Choudhary, S.K., Tripathi, R.D. and Singh, S.N., 2000. Management of fly-ash landfills with Cassia surattensis Burm: A case study. Bulletin of Environmental Contamination and Toxicology, 65(6), pp.675-682. https://doi.org/10.1007/s0012800176.
  46. Walkley, A. and Black, I.A., 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science, 37(1), pp.29-38.
  47. Wright, S.F. and Upadhyaya, A., 1996. Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Science, 161(6), pp.575-586. http://dx.doi.org/10.1097/00010694-199609000-00003.
 

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