اخبار و اعلانات

 آمار

تعداد نشریات282
تعداد نشریات سازمان80
تعداد شماره‌ها3,272
تعداد مقالات36,131
تعداد مشاهده مقاله55,698,186
تعداد دریافت فایل اصل مقاله94,483,282
Khamisabadi, Hassan. (1404). Medicinal Effects of Lemon Balm (Melissa officinalis L.) Supplementation on Growth Performance, Immunity, and Intestinal Health in Broiler Chickens. سامانه مدیریت نشریات علمی, 14(5), 477-483. doi: 10.22034/jmpb.2025.369272.1953
Hassan Khamisabadi. "Medicinal Effects of Lemon Balm (Melissa officinalis L.) Supplementation on Growth Performance, Immunity, and Intestinal Health in Broiler Chickens". سامانه مدیریت نشریات علمی, 14, 5, 1404, 477-483. doi: 10.22034/jmpb.2025.369272.1953
Khamisabadi, Hassan. (1404). 'Medicinal Effects of Lemon Balm (Melissa officinalis L.) Supplementation on Growth Performance, Immunity, and Intestinal Health in Broiler Chickens', سامانه مدیریت نشریات علمی, 14(5), pp. 477-483. doi: 10.22034/jmpb.2025.369272.1953
Khamisabadi, Hassan. Medicinal Effects of Lemon Balm (Melissa officinalis L.) Supplementation on Growth Performance, Immunity, and Intestinal Health in Broiler Chickens. سامانه مدیریت نشریات علمی, 1404; 14(5): 477-483. doi: 10.22034/jmpb.2025.369272.1953

Medicinal Effects of Lemon Balm (Melissa officinalis L.) Supplementation on Growth Performance, Immunity, and Intestinal Health in Broiler Chickens

Journal of Medicinal Plants and By-products
مقاله 14، دوره 14، شماره 5، آذر و دی 2025، صفحه 477-483    اصل مقاله (839.09 K)
نوع مقاله: Research Article
شناسه دیجیتال (DOI): 10.22034/jmpb.2025.369272.1953
نویسنده
Hassan Khamisabadi*
Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
چکیده
Lemon balm (Melissa officinalis L.) is a medicinal plant rich in bioactive compounds such as essential oils, phenolic acids, flavonoids, and triterpenes, which are known for their antioxidant, anti-inflammatory, antimicrobial, and immunomodulatory properties. This study aimed to evaluate the effects of dietary Lemon balm (LB) supplementation on growth performance, humoral immunity, and intestinal health in broiler chickens. Four hundred male Ross 308 broiler chicks were randomly assigned to five dietary treatments: a basal diet (control), basal diet plus 250 ppm lincomycin (antibiotic), and basal diet supplemented with 1%, 2%, or 3% LB powder. The experimental period lasted 42 days. Results showed that 1% LB supplementation significantly improved economic efficiency by reducing feed cost per kg weight gain and increasing return on investment. LB supplementation, particularly at 1% and 3%, enhanced antibody titers against Newcastle disease and avian influenza, respectively, indicating improved humoral immunity. All levels of LB reduced serum aspartate aminotransferase (AST), uric acid, and creatinine, suggesting better hepatic and renal health. Both antibiotic and LB groups exhibited decreased ileal E. coli counts, while no adverse effects on carcass yield or beneficial intestinal Lactobacillus populations were observed. However, 3% LB reduced villus height, indicating potential negative effects at higher doses. In conclusion, M. officinalis demonstrates promising potential as a phytogenic feed additive, offering health and economic benefits for broiler production. Its use as a natural alternative to antibiotics could contribute to safer and more sustainable poultry farming.
کلیدواژه‌ها
Blood metabolites؛ Broilers؛ Immunity؛ Lemon balm؛ Performance
مراجع
  1. Fallah S., Maggi F., Ghanbari-Odivi A., Rostaei M. Optimizing LB (Melissa Officinalis L.) Cultivation: Effects of Different Manures on Plant Growth and Essential Oil Production During Consecutive Harvests. Horticulture. 2024; 10: 1105.
  2. Mathews I.M., Eastwood J., Lamport D.J., Cozannet R.L., Fanca-Berthon P., Williams C.M. Clinical efficacy and tolerability of LB (Melissa officinalis L.) in psychological well-being: a review. Nutrition. 2024; 16: 3545.
  3. De Oliveira I., Chrysargyris A., Heleno SA., Carocho M., Calhelha R.C., Dias M.I., Barros L. Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes. Food Research International. 2023; 170: 113044.
  4. Bessada S.M., Barreira J.C., Barros L., Ferreira I.C., Oliveira M.B.P. Phenolic profile and antioxidant activity of Coleostephus myconis (L.) Rchb. f.: An underexploited and highly disseminated species. Industrial Crops and Products. 2016; 89: 45-51.
  5. Sharifi-Rad J., Quispe C., Herrera-Bravo J., Akram M., Abbaass W., Semwal P., Calina, D. [Retracted] Phytochemical Constituents, Biological Activities, and Health‐Promoting Effects of the Melissa officinalis. Oxidative Medicine and Cellular Longevity 2021; 1: 6584693.
  6. Shakeri A., Sahebkar A., Javadi B. Melissa officinalis L.–A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology. 2016; 188: 204-228.
  7. Üstündağ Ü., Macar O., Kalefetoğlu Macar T., Yalçın E., Çavuşoğlu, K. Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L. Scientific Reports. 2023; 13(1): 22110.
  8. Matkowski A., Jamiolkowska-Kozlowska W., Nawrot, I. Chinese medicinal herbs as source of antioxidant compounds–where tradition meets the Future and Current Medicinal Chemistry. 2013; 20: 984-1004.
  9. Sarris J., Panossian A., Schweitzer I., Stough C., Scholey A. Herbal medicine for depression, anxiety and insomnia: a review of psychopharmacology and clinical evidence. European Neuropsychopharmacology. 2011: 21:841-860.
  10. Abdel-Naime W.A., Fahim J.R., Abdelmohsen U.R., Fouad M.A., Al-Footy KO., Abdel-Lateff AA., Kamel MS. New antimicrobial triterpene glycosides from LB (Melissa officinalis). South African Journal of Botany. 2019; 125: 161-167.
  11. Shakeri A., Sahebkar A., Javadi B. Melissa officinalis L.–A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology. 2016; 188: 204-228.
  12. Sharifi-Rad J., Quispe C., Herrera-Bravo J., Akram M., Abbaass W., Semwal P., Calina D. [Retracted] Phytochemical Constituents, Biological Activities, and Health‐Promoting Effects of the Melissa officinalis. Oxidative Medicine and Cellular Longevity, Phytotherapy Research. 2021; 35:95-121.
  13. Bilan M.V., Lieshchova M.A., Brygadyrenko V.V. Impacts on gut microbiota of rats with high-fat diet supplemented by herbs of Melissa officinalis, Lavandula angustifolia and Salvia officinalis. Regulatory Mechanisms in Biosystems. 2023; 14: 155-160.
  14. Kiani H.S., Ali A., Zahra S., Hassan ZU., Kubra KT., Azam M., Zahid HF. Phytochemical composition and pharmacological potential of lemongrass (cymbopogon) and impact on gut microbiota. Applied Chemistry. 2022; 2: 229-246.
  15. Elkhoriby EE., Radwan HA., Ghanem HM., Fouda MM. Impact of Supplementing a Frankincense-Melissa officinalis Mixture to Drinking Water on the Performance and Economic Efficiency of Two Commercial Japanese Quail Strains. Journal of Advanced Veterinary Research. 2023; 13: 1848-1854.
  16. Islam M.A., Haque M.A., Rahman M.A., Hossen F., Reza M., Barua A., Jakariya M. A review on measures to rejuvenate immune system: Natural mode of protection against coronavirus infection. Frontiers in Immunology. 2022; 13: 837290.
  17. Pourghanbari G., Nili H., Moattari A., Mohammadi A., Iraji A. Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2). Virus Disease. 2016; 27: 170-178.
  18. Astani A., Heidary Navid M., Schnitzler P. Attachment and penetration of acyclovir‐resistant herpes simplex virus are inhibited by Melissa officinalis extract. Phytotherapy Research. 2014; 28: 1547-1552.
  19. Chen SG., Leu Y.L., Cheng M.L., Ting S.C., Liu C.C., Wang S.D., Ho H.Y. Anti-enterovirus 71 activities of Melissa officinalis extract and its biologically active constituent rosmarinic acid. Scientific Reports. 2017; 7: 12264.
  20. Abdinezhad F., Mohammadi M. Effect of LB (Melissa officinalis) aqueous extract on immune response and performance of broilers. Animal Production. 2015; 17: 281-290.
  21. Poorghasemi M., Seidavi A., Mohammadi M., Simões J., Laudadio V., Tufarelli, V. Effect of dietary inclusion of LB (melissa officinalis l.) extract on performance, gut microflora, blood parameters, immunity and carcass traits of broilers. The Journal of Poultry Science. 2017; 54: 263-270.
  22. Posłuszny MA., Chłopecka M., Suor-Cherer S., Cisse S., Amine Benarbia M., Mendel M. Modulation of chicken gut contractility by Melissa officinalis—ex vivo study. Poultry Science. 2023; 102: 103045.
  23. Cunniff P., Washington D. Official methods of analysis of AOAC Int. Journal of AOAC International. 1997; 80: 127A.
  24. Zaghari M., Sarani P., Hajati, H. Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens. Journal of Applied Animal Research. 2020; 48: 166-175.
  25. Talebi A., Maham M., Asri-Rezaei S., Pournaghi P., Khorrami MS., Derakhshan A. Effects of Nigella sativa on performance, blood profiles, and antibody titer against Newcastle disease in broilers. EBCAM. 2021; 1: 2070375.
  26. Ghițǎ M., Petcu C.D., Codreanu I., Gâjâilǎ G., Mihai O.D., Cotor G. Research on the dynamics of erythrocytic series in relation to age, in chickens. Scientific Papers. Series D. Animal Science. 2022; 2: 211-216.
  27. Elsherbeni A.I., Youssef I.M., Kamal M., Youssif M.A., El-Gendi G.M., El-Garhi O.H., Hamouda R.E. Impact of Adding Zeolite to Broilers' Diet and Litter on Growth, Blood Parameters, Immunity, and Ammonia Emission. Poultry Science. 2024; 103981.
  28. Selim S., Abdel-Megeid N.S., Alhotan R.A., Ebrahim A., Hussein E. Nutraceuticals vs. antibiotic growth promoters: differential impacts on performance, meat quality, blood lipids, cecal microbiota, and organ histomorphology of broiler chicken. Poultry Science. 2024; 103971.
  29. Basit M.A., Kadir A.A., Loh T.C., Abdul Aziz S., Salleh A., Zakaria Z.A., Banke Idris S. Comparative efficacy of selected phytobiotics with halquinol and tetracycline on gut morphology, ileal digestibility, cecal microbiota composition and growth performance in broiler chickens. Animals. 2020; 10: 2150.
  30. Hajati H., Hassanabadi A., Golian A.G., Nassiri Moghaddam H., Nassiri MR. The effect of grape seed extract and vitamin C feed supplements carcass characteristics, gut morphology and ileal microflora in broiler chickens exposed to chronic heat stress. Journal of Applied Animal Science. 2015; 5: 155-165.
  31. Brenes A., Roura, E. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science Technology. 2010; 158: 1-14.
  32. Aminullah N., Mostamand A., Zahir A., Mahaq O., Azizi M.N. Phytogenic feed additives as alternatives to antibiotics in poultry production: A review. Veterinary World. 2025; 18(1).
  33. Zead G.A.A., Elazab S.T., Hassan A.E., Ateya A.I., Amer M.S. The anti-inflammatory and antioxidant effects of Melissa officinalis (Lemon balm) on paracetamol induced hepatotoxicity in broiler chickens. Mansoura Veterinary Medicine Journal. 2024; 25: 5.
  34. Petrovic V., Marcincak S., Popelka P., Simkova J., Martonova M., Buleca J., Kovac G. The effect of supplementation of clove and agrimony or clove and LB on growth performance, antioxidant status and selected indices of lipid profile of broiler chickens. Journal of Animal Physiology and Animal Nutrition. 2012; 96: 970-977.
  35. Kwiecieñ M., Winiarska-Mieczan A. Effect of addition of herbs on body weight and assessment of physical and chemical alterations in the tibia bones of broiler chickens. Journal of Elements. 2009; 14: 263-270.
  36. Wang J., Su S., Pender C., Murugesan R., Syed B., Kim W.K. Effect of a phytogenic feed additive on growth performance, nutrient digestion, and immune response in broiler-fed diets with two different levels of crude protein. Animals. 2021; 11: 775.
  37. Sapsuha Y., Syafie Y., Sjafani N., Ishak H. Campus Intellectual Product Business Development Program (PPUPIK): Utilization of Herbs as Phytobiotic in Broiler Chicken. In Journal of Physics: Conference Series. IOP Publishing. 2019; 1364:012078.
  38. Nobakht A., Moghaddam M. The effects of using different levels of LB (Melissa officinalis L.) medicinal plant on performance, egg traits, blood biochemical parameters and Immunity cells of laying hens. African Journal of Agricultural Research. 2012; 7: 1682-1686.
  39. Oblakova M., Sotirov L., Lalev M., Koynarski T., Bozakova N., Hristakieva P., Ivanova I. Effect of some medicinal herbs on natural humoral immunity in turkeys. J. Hellenic. Journal of Hellenic Veterinary Medical Society. 2022; 73: 3673-3680.
  40. Zead G.A.A., Elazab S.T., Hassan A.E., Ateya A.I., Amer M.S. The anti-inflammatory and antioxidant effects of Melissa officinalis (LB) on paracetamol induced hepatotoxicity in broiler chickens. Mansoura Veterinary Medical Journal. 2024; 25: 5.
  41. Zarei A., Ashtiyani SC., Taheri S., Rasekh, F. Comparison between effects of different doses of Melissa officinalis and atorvastatin on the activity of liver enzymes in hypercholesterolemia rats. Asian Journal of Pharmaceutics. 2014; 4: 15.
  42. Li Z., Wu J., Zhao Y., Song J., Wen, Y. Natural products and dietary interventions on liver enzymes: an umbrella review and evidence map. Frontiers in Nutrition. 2024; 11: 1300860.
  43. Marcinčák S., Mesarčová L., Popelka P., Čertík M., Šimková J., Marcinčáková D., Mártonová M. The influence of dietary supplementation with Melissa officinalis and combination of Achillea millefolium and Crataegus oxyacantha on oxidative stability of stored poultry meat. Journal of Animal Feed Science. 2011; 20: 236-245.
  44. Shahbazi H.R., Heidari M. The comparison effects of different levels of extract of Medicinal plants Melissa officinalis on efficiency, and safety carcass specification in broiler chickens. Biological Forum- An International Journal. 2015; 7: 1041-1044.
  45. Elbaz A.M., Ashmawy E.S., Salama A.A., Abdel-Moneim A.M.E., Badri F.B., Thabet H.A. Effects of garlic and lemon essential oils on performance, digestibility, plasma metabolite, and intestinal health in broilers under environmental heat stress. BMC. Veterinary Research. 2022; 18: 430.
  46. Park J.H., Kim I.H. Effects of a protease and essential oils on growth performance, blood cell profiles, nutrient retention, ileal microbiota, excreta gas emission, and breast meat quality in broiler chicks. Poultry Science. 2018; 97: 2854-2860.
  47. Su G., Zhou X., Wang Y., Chen D., Chen G., Li Y., He J. Effects of plant essential oil supplementation on growth performance, immune function and antioxidant activities in weaned pigs. Lipids in Health and Disease. 2018; 17: 1-10.
  48. Diaz-Sanchez S., D'Souza D., Biswas D., Hanning I. Botanical alternatives to antibiotics for use in organic poultry production. Poultry Science. 2015; 94: 1419-1430.
  49. Ahsan U., Kuter E., Raza I., Köksal B.H., Cengiz Ö., Yıldız M., Sevim Ö. Dietary supplementation of different levels of phytogenic feed additive in broiler diets: the dynamics of growth performance, caecal microbiota, and intestinal morphometry. British Journal of Poultry Science. 2018; 20: 737-746.
  50. Latek U., Chłopecka M., Karlik W., Mendel M. Phytogenic compounds for enhancing intestinal barrier function in poultry–a review. Planta Medica, 88(03/04). 2022; 218-236.
  51. Patra A.K., Amasheh S., Aschenbach JR. Modulation of gastrointestinal barrier and nutrient transport function in farm animals by natural plant bioactive compounds–a comprehensive review. Critical Reviews in Food Science and Nutrition. 2019; 59: 3237-3266.
  52. Zhang R., Yang J., Wang Q., Hu D., Zhao Q., Zhu S., Dong H. Comparative Efficacy of Plant Extracts and Probiotics on Growth and Gut Health in Chickens with Necrotic Enteritis. Animals: an Open Access Journal from MDPI. 2024; 14: 3312.
  53. Khamisabadi H. The effects of different levels of Melissa officinalis (Lemon balm) extract in the diet of broiler chickens. [In Persian]. Final Report. Agricultural Research, Education and Extension Organization (AREEO). 2015; 52039.
  54. Murugesan G.R., Syed B., Haldar S., Pender C. Phytogenic feed additives as an alternative to antibiotic growth promoters in broiler chickens. Frontiers in Veterinary Science. 2015; 2: 21.
آمار
تعداد مشاهده مقاله: 614
تعداد دریافت فایل اصل مقاله: 612


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب