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Green Synthesis of Manganese Oxide Nanoparticles Using Basil Extract for Biocompatibility and Therapeutic Targeting of Metribuzin-poisoned Heart and Lung Tissues in Wistar Rats | ||
| Archives of Razi Institute | ||
| مقاله 16، دوره 81، شماره 1، فروردین و اردیبهشت 2026، صفحه 137-146 اصل مقاله (1.83 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ARI.81.1.3602 | ||
| نویسندگان | ||
| islam Boulaares1؛ Samir Derouiche* 1؛ Sara Chetehouna1؛ Janetta Niemann2 | ||
| 1Department of Cellular and Molecular Biology, Faculty of the Sciences of Nature and Life, El-Oued University, El-Oued, Algeria. | ||
| 2Department of Genetic, Faculty of Agriculture, Poznan University of Life Science, Poznan, Poland. | ||
| چکیده | ||
| Introduction: Manganese oxide nanoparticles (MnO NPs) have garnered interest for use in a variety of fields, such as biomedical applications, including cancer theranostics and drug delivery. This work aimed to investigate the potential therapeutic and preventive benefits of green-synthesized MnO NPs made from basil extract against metribuzin-induced oxidative stress, metabolic toxicity, inflammation, and histological changes in the lungs and heart. Materials & Methods: The green synthesis of MnO NPs using basil extract was performed. The shape and size distribution of the MnO NPs were analyzed using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Eighteen male albino Wistar rats were divided into three groups (n=6), which consisted of a control group, a metribuzin-treated group, and a MnO NPs-treated group. The evaluation of the oxidative stress status by measuring the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), Glutathione S-transferase (GSTs), catalase (CAT), glutathione (GSH), and malondialdehyde (MDA) in the lungs and heart tissues. Additionally, the tissue histology of the organs was examined, and various biochemical parameters (aminotransferase [GOT], lactate dehydrogenase [LDH], and creatine phosphokinase [CPK]) and inflammation markers (white blood cells [WBC], lymphocytes [lymph], Mid-sized cells [MID], and granulocytes [Gran]). were estimated. The green synthesis of MnO NPs was indicated by the gradual shift in color from golden yellow to dark brown. The morphological characteristics and particle size distribution of the MnO NPs were identified using TEM and SEM. Results: The analyses revealed that the MnO NPs were uniformly spherical in shape, with an average particle size of 6.52±0.88 nm. Results of the in vivo rats study showed that treatment with metribuzin induced an increase in organs weight, oxidative stress, biochemical toxicity, inflammation, and histological changes in the lungs and heart, as well as a significant ameliorative effect of MnO NPs against the toxic effects induced by metribuzin by reversing all of the aforementioned parameters. Conclusion: In conclusion, the results of the in vivo investigation showed that rats given the metribuzin herbicide suffered from organ weight gain, oxidative stress, biochemical toxicity, inflammation, and histological alterations in their lungs and heart. Additionally, MnO NPs showed effective therapeutic and preventive actions against lung and heart damage caused by metribuzin. Basil extract’s phytochemical components enhance MnO NP biocompatibility, reduce toxicity, and provide antioxidant and anti-inflammatory properties, making them safe and therapeutic for biomedical applications. | ||
| کلیدواژه‌ها | ||
| Heart؛ Inflammation؛ Lungs؛ Metribuzin؛ Manganese oxide nanoparticles (MnO NPs)؛ Oxidative stress | ||
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