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Dahdouh, Faouzi, Chainez Djeffal, Hanane, Bourouba, Amira, Bousba, Rania, Bourouba, Amina, Djebar - Berrebbah, Houria. (1404). Apis meliffera intermissa venom attenuated colitis-associated cancer progression in azoxymethane treated mice. سامانه مدیریت نشریات علمی, 80(3), 711-718. doi: 10.32592/ARI.2025.80.3.711
Faouzi Dahdouh; Hanane Chainez Djeffal; Amira Bourouba; Rania Bousba; Amina Bourouba; Houria Djebar - Berrebbah. "Apis meliffera intermissa venom attenuated colitis-associated cancer progression in azoxymethane treated mice". سامانه مدیریت نشریات علمی, 80, 3, 1404, 711-718. doi: 10.32592/ARI.2025.80.3.711
Dahdouh, Faouzi, Chainez Djeffal, Hanane, Bourouba, Amira, Bousba, Rania, Bourouba, Amina, Djebar - Berrebbah, Houria. (1404). 'Apis meliffera intermissa venom attenuated colitis-associated cancer progression in azoxymethane treated mice', سامانه مدیریت نشریات علمی, 80(3), pp. 711-718. doi: 10.32592/ARI.2025.80.3.711
Dahdouh, Faouzi, Chainez Djeffal, Hanane, Bourouba, Amira, Bousba, Rania, Bourouba, Amina, Djebar - Berrebbah, Houria. Apis meliffera intermissa venom attenuated colitis-associated cancer progression in azoxymethane treated mice. سامانه مدیریت نشریات علمی, 1404; 80(3): 711-718. doi: 10.32592/ARI.2025.80.3.711

Apis meliffera intermissa venom attenuated colitis-associated cancer progression in azoxymethane treated mice

Archives of Razi Institute
مقاله 20، دوره 80، شماره 3، مرداد و شهریور 2025، صفحه 711-718    اصل مقاله (615.16 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.3.711
نویسندگان
Faouzi Dahdouh* 1، 2؛ Hanane Chainez Djeffal1؛ Amira Bourouba3؛ Rania Bousba3؛ Amina Bourouba3؛ Houria Djebar - Berrebbah1
1Department of Biology, Faculty of Sciences, Badji-Mokhtar, Annaba University, Annaba, BP 23000, Algeria.
2Department of Natural Sciences, Higher School of Technological Education, Skikda 21000, Algeria.
3Department of Biology, Faculty of Sciences, Badji-Mokhtar, Annaba University, Annaba, BP 23000, Algeria
چکیده
Colitis-associated colorectal cancer (CAC) is a serious condition driven by chronic inflammation in the colon, representing a significant challenge in both preventative and therapeutic contexts. Apis mellifera intermissa venom has shown promising therapeutic potential in various disease models, particularly those involving inflammation and tumorigenesis. This study evaluates the therapeutic effects of venom derived from honeybees native to Algeria on the progression of CAC in azoxymethane (AOM)-treated mice. A total of 28 male mice were randomly allocated into four groups (n=7 per group): a control group received a tap drinking water, an AOM group (10 mg AOM /kg body weight), a bee venom group (0.76 mg/kg body weight), and a combined bee venom + AOM group. CAC was induced in mice by a single intraperitoneal injection (i.p) of AOM, and a high-fat diet (45% fat by diet weight) for two weeks. The potential therapeutic effect was evaluated by administering bee venom intraperitoneally on a daily basis for two weeks. AOM significantly reduced body weight, food and water intake while increasing colon weight. Hematological analysis revealed significant reductions in red blood cells (RBC), hemoglobin (HGB), and hematocrit (HCT), coupled with increased white blood cell counts, mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). Elevated serum C-reactive protein (CRP) levels further confirmed systemic inflammation. Macroscopic examination and histopathological analyses of the colon revealed extensive pathological changes in the AOM group, including severe mucosal inflammation, necrotic epithelial damage, and substantial immune cell infiltration. Noteworthy, co-treatment with bee venom effectively mitigated these pathological alterations. Bee venom significantly restored hematological profiles by improving RBC count, HGB, and HCT levels while reducing the elevated WBC count, MCV, and MCH values. CRP levels were significantly reduced, reflecting the anti-inflammatory effects of the venom. Also, macroscopic evaluations demonstrated the preservation of colon morphology, while histopathological assessments revealed an improved epithelial integrity with fewer signs of necrosis and cellular atypia. These findings suggest that Apis mellifera intermissa venom holds potential as an adjunct therapeutic agent for suppressing CAC progression, warranting further investigation into its underlying mechanisms and clinical applicability.
کلیدواژه‌ها
Colitis-associated cancer؛ Azoxymethane؛ Hematological parameters؛ Apis mellifera intermissa venom؛ mice
مراجع
  1. Grivennikov SI. Inflammation and colorectal cancer: colitis-associated neoplasia. In: Seminars in immunopathology. Springer; 2013. p. 229–44.
  2. Roessner A, Kuester D, Malfertheiner P, Schneider-Stock R. Oxidative stress in ulcerative colitis-associated carcinogenesis. Pathol Pract. 2008;204(7):511–24.
  3. Piechota-Polanczyk A, Fichna J. The role of oxidative stress in pathogenesis and treatment of inflammatory bowel diseases. Naunyn Schmiedebergs Arch Pharmacol. 2014;387:605–20.
  4. Greten FR, Eckmann L, Greten TF, Park JM, Li ZW, Egan LJ, et al. IKKβ links inflammation and tumorigenesis in a mouse model of colitis-associated cancer. Cell. 2004;118(3):285–96.
  5. Ullah A, Aldakheel FM, Anjum SI, Raza G, Khan SA, Gajger IT. Pharmacological properties and therapeutic potential of honey bee venom. Saudi Pharm J. 2023;31(1):96–109.
  6. Haddad NJ, Loucif-Ayad W, Adjlane N, Saini D, Manchiganti R, Krishnamurthy V, et al. Draft genome sequence of the Algerian bee Apis mellifera intermissa. Genomics data. 2015;4:24–5.
  7. Mirzavi F, Saghi H, Ebrahimi S, Mahdinezhad MR, Hosseini H, Soukhtanloo M. The Anti-tumor, Anti-oxidative, and Anti-inflammatory Effects of Bee Venom in C26 Colon Carcinoma-Bearing Mice Model. Iran J Sci. 2024;48(1):69–75.
  8. Aliyazicioglu R. Therapeutic effects of bee venom. Chem Sci Int J. 2019;26(1):1–5.
  9. Dahdouh F, Belhamzaoui K, Aouadi L, Aldahmash W, Harrath AH, Plavan G, et al. Bee Venom causes oxidative stress, biochemical and histopathological changes in the kidney of mice. Physiol Res. 2023;72(4):455.
  10. Drury RAB, Wallington EA. Carleton’s histological technique 5th ed. New York Churchill Livingstone. 1980;
  11. Kubota M, Shimizu M, Sakai H, Yasuda Y, Terakura D, Baba A, et al. Preventive effects of curcumin on the development of azoxymethane-induced colonic preneoplastic lesions in male C57BL/KsJ-db/db obese mice. Nutr Cancer. 2012;64(1):72–9.
  12. Tak KH, Ahn E, Kim E. Increase in dietary protein content exacerbates colonic inflammation and tumorigenesis in azoxymethane-induced mouse colon carcinogenesis. Nutr Res Pract. 2017;11(4):281–9.
  13. Tanaka T. Development of an inflammation‐associated colorectal cancer model and its application for research on carcinogenesis and chemoprevention. Int J Inflam. 2012;2012(1):658786.
  14. Tanaka T. Preclinical cancer chemoprevention studies using animal model of inflammation-associated colorectal carcinogenesis. Cancers (Basel). 2012;4(3):673–700.
  15. Hwang S, Jo M, Hong JE, Park CO, Lee CG, Rhee KJ. Protective effects of zerumbone on colonic tumorigenesis in enterotoxigenic Bacteroides fragilis (ETBF)-colonized AOM/DSS BALB/c mice. Int J Mol Sci. 2020;21(3):857.
  16. Kangawa Y, Yoshida T, Maruyama K, Okamoto M, Kihara T, Nakamura M, et al. Cilostazol and enzymatically modified isoquercitrin attenuate experimental colitis and colon cancer in mice by inhibiting cell proliferation and inflammation. Food Chem Toxicol. 2017;100:103–14.
  17. Abbas HA, Abass DA. Amlodipine (P-glycoprotein) inhibitor modulation effect on the Hematological, Oxidative and Inflammatory effects of Doxorubicin in Colorectal cancer induced in Mice. J Surv Fish Sci. 2023;10(3S):823–33.
  18. Shimpo K, Beppu H, Chihara T, Kaneko T, Shinzato M, Sonoda S. Effects of Aloe arborescens ingestion on azoxymethane-induced intestinal carcinogenesis and hematological and biochemical parameters of male F344 rats. Asian Pacific J Cancer Prev. 2006;7(4):585.
  19. Langley RE, Burdett S, Tierney JF, Cafferty F, Parmar MKB, Venning G. Aspirin and cancer: has aspirin been overlooked as an adjuvant therapy? Br J Cancer. 2011;105(8):1107–13.
  20. Terzić J, Grivennikov S, Karin E, Karin M. Inflammation and colon cancer. Gastroenterology. 2010;138(6):2101–14.
  21. Gajski G, Leonova E, Sjakste N. Bee Venom: Composition and Anticancer Properties. Toxins (Basel). 2024;16(3):117.
  22. Son DJ, Lee JW, Lee YH, Song HS, Lee CK, Hong JT. Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds. Pharmacol Ther. 2007;115(2):246–70.
  23. Kim SD, Kim JH, Kim DH, Park JH, Gong Y, Sun C, et al. Comprehensive Evaluation of Traditional Herbal Medicine Combined With Adjuvant Chemotherapy on Post-Surgical Gastric Cancer: A Systematic Review and Meta-Analysis. Integr Cancer Ther. 2024;23:15347354231226256.
  24. Zhang H, Wang M, Zhou Y, Bao S, Wang F, Li C. Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice. Mar Drugs. 2024;22(10):469.
  25. Farombi EO, Ajayi BO, Ajeigbe OF, Maruf OR, Anyebe DA, Opafunso IT, et al. Mechanistic exploration of 6-shogaol’s preventive effects on azoxymethane and dextran sulfate sodium-induced colorectal cancer: involvement of cell proliferation, apoptosis, carcinoembryonic antigen, wingless-related integration site signaling, and oxido-. Toxicol Mech Methods. 2024;1–10.
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