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Taghyan, Salma, Mahmoud, Elham, Taha, Rasha, Shamel, Mohamed. (1404). Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study. سامانه مدیریت نشریات علمی, 80(5), 1209-1216. doi: 10.66224/ARI.80.5.3494
Salma Awad Taghyan; Elham Fathy Mahmoud; Rasha Mohamed Taha; Mohamed Shamel. "Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study". سامانه مدیریت نشریات علمی, 80, 5, 1404, 1209-1216. doi: 10.66224/ARI.80.5.3494
Taghyan, Salma, Mahmoud, Elham, Taha, Rasha, Shamel, Mohamed. (1404). 'Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study', سامانه مدیریت نشریات علمی, 80(5), pp. 1209-1216. doi: 10.66224/ARI.80.5.3494
Taghyan, Salma, Mahmoud, Elham, Taha, Rasha, Shamel, Mohamed. Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study. سامانه مدیریت نشریات علمی, 1404; 80(5): 1209-1216. doi: 10.66224/ARI.80.5.3494

Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study

Archives of Razi Institute
مقاله 15، دوره 80، شماره 5، آذر و دی 2025، صفحه 1209-1216    اصل مقاله (5.13 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.66224/ARI.80.5.3494
نویسندگان
Salma Awad Taghyan* 1؛ Elham Fathy Mahmoud2؛ Rasha Mohamed Taha2؛ Mohamed Shamel1
1Department of Oral Biology, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt.
2Department of Oral Biology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
چکیده
Introduction: Acrylamide (AA) is a chemical compound that poses a major public health concern. This study aimed to evaluate the protective effect of Nigella sativa (NS) oil against AA- induced toxicity on the submandibular salivary glands (SMGs) of Albino rats. 
Materials & Methods: Thirty male albino rats weighing 150–200 g were equally and randomly divided into some groups: Control group received normal saline vehicle daily via oral gavage for 30 days, AA group received 15 mg/kg body weight of AA dissolved in 0.2 mL saline solution daily via oral gavage for 30 days. NS group received 15 mg/kg body weight (bw) of AA combined with 1 mL/kg bw of NS oil daily via oral gavage for 30 days. At the end of the experiment, rats were euthanized, and SMGs were dissected for histological evaluation, including hematoxylin and eosin staining (H&E) and immunohistochemistry for inducible nitric oxide synthase (iNOS), as well as analysis for heme oxygenase-1 (HO-1) expression using real-time polymerase chain reaction (RT-qPCR). 
Results: The acinar and ductal cells of SMG of the AA group showed signs of degeneration and toxicity in the form of ill-defined outlines, pyknotic and crescent-shaped nuclei with different-sized cytoplasmic vacuolations. These changes were statistically significant with increased iNOS immunoexpression and HO-1 gene expression (P<0.0001). Administration of NS alleviated the toxic effect, downregulating both iNOS and HO-1 gene expression. The study revealed a significant cytotoxic effect of AA on SMGs of albino rats (P<0.05), presumably by the generation of oxidative stresses and mitochondrial dysfunction.
Conclusion: NS effectively mitigated these toxic effects, suggesting its potential as a natural antioxidant.
کلیدواژه‌ها
Salivary glands؛ Acrylamide؛ Nigella sativa؛ Oxidative stress
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