اخبار و اعلانات

 آمار

تعداد نشریات282
تعداد نشریات سازمان80
تعداد شماره‌ها3,230
تعداد مقالات35,784
تعداد مشاهده مقاله54,006,541
تعداد دریافت فایل اصل مقاله93,166,048
Taghyan, Salma, Mahmoud, Elham, Taha, Rasha, Shamel, Mohamed. (1404). Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study. سامانه مدیریت نشریات علمی, 80(5), 1209-1216. doi: 10.66224/ARI.80.5.3494
Salma Awad Taghyan; Elham Fathy Mahmoud; Rasha Mohamed Taha; Mohamed Shamel. "Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study". سامانه مدیریت نشریات علمی, 80, 5, 1404, 1209-1216. doi: 10.66224/ARI.80.5.3494
Taghyan, Salma, Mahmoud, Elham, Taha, Rasha, Shamel, Mohamed. (1404). 'Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study', سامانه مدیریت نشریات علمی, 80(5), pp. 1209-1216. doi: 10.66224/ARI.80.5.3494
Taghyan, Salma, Mahmoud, Elham, Taha, Rasha, Shamel, Mohamed. Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study. سامانه مدیریت نشریات علمی, 1404; 80(5): 1209-1216. doi: 10.66224/ARI.80.5.3494

Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study

Archives of Razi Institute
مقاله 15، دوره 80، شماره 5، آذر و دی 2025، صفحه 1209-1216    اصل مقاله (5.13 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.66224/ARI.80.5.3494
نویسندگان
Salma Awad Taghyan* 1؛ Elham Fathy Mahmoud2؛ Rasha Mohamed Taha2؛ Mohamed Shamel1
1Department of Oral Biology, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt.
2Department of Oral Biology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
چکیده
Introduction: Acrylamide (AA) is a chemical compound that poses a major public health concern. This study aimed to evaluate the protective effect of Nigella sativa (NS) oil against AA- induced toxicity on the submandibular salivary glands (SMGs) of Albino rats. 
Materials & Methods: Thirty male albino rats weighing 150–200 g were equally and randomly divided into some groups: Control group received normal saline vehicle daily via oral gavage for 30 days, AA group received 15 mg/kg body weight of AA dissolved in 0.2 mL saline solution daily via oral gavage for 30 days. NS group received 15 mg/kg body weight (bw) of AA combined with 1 mL/kg bw of NS oil daily via oral gavage for 30 days. At the end of the experiment, rats were euthanized, and SMGs were dissected for histological evaluation, including hematoxylin and eosin staining (H&E) and immunohistochemistry for inducible nitric oxide synthase (iNOS), as well as analysis for heme oxygenase-1 (HO-1) expression using real-time polymerase chain reaction (RT-qPCR). 
Results: The acinar and ductal cells of SMG of the AA group showed signs of degeneration and toxicity in the form of ill-defined outlines, pyknotic and crescent-shaped nuclei with different-sized cytoplasmic vacuolations. These changes were statistically significant with increased iNOS immunoexpression and HO-1 gene expression (P<0.0001). Administration of NS alleviated the toxic effect, downregulating both iNOS and HO-1 gene expression. The study revealed a significant cytotoxic effect of AA on SMGs of albino rats (P<0.05), presumably by the generation of oxidative stresses and mitochondrial dysfunction.
Conclusion: NS effectively mitigated these toxic effects, suggesting its potential as a natural antioxidant.
کلیدواژه‌ها
Salivary glands؛ Acrylamide؛ Nigella sativa؛ Oxidative stress
سایر فایل های مرتبط با مقاله
مراجع

References

  1. Elhelaly AE, AlBasher G, Alfarraj S, Almeer R, Bahbah EI, Fouda MM, et al. Protective effects of hesperidin and diosmin against acrylamide-induced liver, kidney, and brain oxidative damage in rats. Environ Sci Pollut Res Int. 2019; 26(34):35151-62. [DOI:10.1007/s11356-019-06660-3] [PMID]
  2. Dahran N, Abd-Elhakim YM, Mohamed AA, Abd-Elsalam MM, Said EN, Metwally MM, et al. Palliative effect of Moringa olifera-mediated zinc oxide nanoparticles against acrylamide-induced neurotoxicity in rats. Food Chem Toxicol. 2023; 171:113537. [DOI:10.1016/j.fct.2022.113537] [PMID]
  3. Zhao M, Zhang B, Deng L. The mechanism of acrylamide-induced neurotoxicity: Current status and future perspectives. Front Nutr. 2022; 9:859189. [DOI:10.3389/fnut.2022.859189] [PMID]
  4. Abdel-Daim MM, Abo El-Ela FI, Alshahrani FK, Bin-Jumah M, Al-Zharani M, Almutairi B, et al. Protective effects of thymoquinone against acrylamide-induced liver, kidney and brain oxidative damage in rats. Environ Sci Pollut Res. 2020; 27:37709-17. [DOI:10.1007/s11356-020-09516-3]
  5. Mahmoud EF, Zahran DH, Mahmoud MF. Histological, immunohistochemical and ultrastructural evaluation of the cytotoxic effect of acrylamide on submandibular salivary glands in rats. Dent Journal. 2013; 59(4):3775-85. [Link]
  6. Al-Serwi RH, Ghoneim FM. The impact of vitamin E against acrylamide induced toxicity on skeletal muscles of adult male albino rat tongue: Light and electron microscopic study. J Microsc Ultrastruct. 2015; 3(3):137-47. [DOI:10.1016/j.jmau.2015.03.001] [PMID]
  7. Amin B, Hosseinzadeh H. Black cumin (Nigella sativa) and its active constituent, thymoquinone: An overview on the analgesic and anti-inflammatory effects. Planta Med. 2016; 82(1-2):8-16. [DOI:10.1055/s-0035-1557838] [PMID]
  8. Hannan MA, Rahman MA, Sohag AAM, Uddin MJ, Dash R, Sikder MH, et al. Black cumin (Nigella sativa L.): A comprehensive review on phytochemistry, health benefits, molecular pharmacology, and safety. Nutrients. 2021; 13(6):1784. [DOI:10.3390/nu13061784] [PMID]
  9. Faul F, Erdfelder E, Buchner A, Lang AG. Statistical power analyses using G* Power 3.1: Tests for correlation and regression analyses. Behav Res Methods. 2009; 41(4):1149-60.[DOI:10.3758/BRM.41.4.1149] [PMID]
  10. Mohamadin AM, Sheikh B, Abd El-Aal AA, Elberry AA, Al-Abbasi FA. Protective effects of Nigella sativa oil on propoxur-induced toxicity and oxidative stress in rat brain regions. Pestici Biochem Physiol. 2010; 98(1):128-34. [DOI:10.1016/j.pestbp.2010.05.011]
  11. Shamel M, Baz S, Mahmoud H, Taghyan SA, Bakr MM, Al Ankily M. Balancing Risks versus Benefits: Vitamin C Therapy versus Copper Oxide Nanoparticles Toxicity in Albino Rats’ Submandibular Salivary Gland. Eur J Dent. 2025; 19(1):124-32. [DOI:10.1055/s-0044-1786867] [PMID]
  12. Sparmann G, Jäschke A, Loehr M, Liebe S, Emmrich J. Tissue homogenization as a key step in extracting RNA from human and rat pancreatic tissue. Biotechniques. 1997; 22(3):408-12. [DOI:10.2144/97223bm07] [PMID]
  13. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods. 2001; 25(4):402-8. [DOI:10.1006/meth.2001.1262] [PMID]
  14. Elsawi NM, Abo Kresha SAT, Mohamed MA, Khorshed A, Aldajani W, Rajeh NA, et al. Curcumin Ameliorates Acrylamide Induced Ovarian Toxicity in Albino Female Rats: A Biochemical and Histological Study. Egypt J Chem. 2023; 66(3):157-68. [DOI:10.21608/ejchem.2022.139554.6136]
  15. Al-Serwi RH, Anees MM, Abd Elhamied AS. Biological effect of acrylamide on parotid salivary gland of Albino rats. Egypt Dent J. 2016; 62(1):37-45. [Link]
  16. Bakr MM, Al-Ankily MM, Shogaa SM, Shamel M. Attenuating effect of vitamin E against Silver Nano Particles Toxicity in Submandibular Salivary Glands. Bioengineering. 2021; 8(12):219. [DOI:10.3390/bioengineering8120219] [PMID]
  17. Liu Z, Song G, Zou C, Liu G, Wu W, Yuan T, et al. Acrylamide induces mitochondrial dysfunction and apoptosis in BV-2 microglial cells. Free Radic Biol Med. 2015; 84:42-53. [DOI:10.1016/j.freeradbiomed.2015.03.013] [PMID]
  18. Hamza SA, Aly HM, Soliman SO, Abdallah DM. Ultrastructural study of the effect of zinc oxide nanoparticles on rat parotid salivary glands and the protective role of quercetin. Alex Dent J. 2016; 41(3):232-7. [DOI:10.21608/adjalexu.2016.58053]
  19. Cinelli MA, Do HT, Miley GP, Silverman RB. Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 2020; 40(1):158-89. [DOI:10.1002/med.21599] [PMID]
  20. Facchinetti MM. Heme-Oxygenase-1. Antioxid Redox Signal. 2020; 32(17):1239-42. [DOI:10.1089/ars.2020.8065] [PMID]
  21. Campbell NK, Fitzgerald HK, Dunne A. Regulation of inflammation by the antioxidant haem oxygenase 1. Nat Rev Immunol. 2021; 21(7):411-25. [DOI:10.1038/s41577-020-00491-x] [PMID]
  22. Ayuob NN. Histological and immunohistochemical study on the possible ameliorating effects of thymoquinone on the salivary glands of rats with experimentally induced hypothyroidism. Egypt J Histol. 2016; 39(2):125-35. [DOI:10.1097/01.EHX.0000489145.55478.51]
  23. Hatipoğlu D, Özsan M, Dönmez HH, Dönmez N. Hepatoprotective effects of nigella sativa oil against acrylamide-induced liver injury in rats. Ankara Üniv Vet Fak Derg. 2023; 70(4):1-22. [DOI:10.33988/auvfd.1096306]
  24. Fathy M, Nikaido T. In vivo modulation of iNOS pathway in hepatocellular carcinoma by Nigella sativa. Environ Health Prev Med. 2013; 18(5):377-85. [DOI:10.1007/s12199-013-0336-8] [PMID]
  25. Montazeri RS, Fatahi S, Sohouli MH, Abu‐Zaid A, Santos HO, Găman MA, et al. The effect of nigella sativa on biomarkers of inflammation and oxidative stress: A systematic review and meta‐analysis of randomized controlled trials. J Food Biochem. 2021; 45(4):e13625. [DOI:10.1111/jfbc.13625] [PMID]
  26. Ahmad A, Alkharfy KM, Jan BL, Ahad A, Ansari MA, Al-Jenoobi FI, et al. Thymoquinone treatment modulates the Nrf2/HO-1 signaling pathway and abrogates the inflammatory response in an animal model of lung fibrosis. Exp Lung Res. 2020; 46(3-4):53-63. [DOI:10.1080/01902148.2020.1726529] [PMID]
  27. Salim EI. Gene profiling cdna microarray analysis of rat colon carcinogenesis treated with crude nigella sativa oil. Egypt J Exp Biol (Zool.). 2015; 7(2):213-21. [Link]
آمار
تعداد مشاهده مقاله: 436
تعداد دریافت فایل اصل مقاله: 468


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب