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Protective Effects of Nigella Sativa Against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study | ||
| Archives of Razi Institute | ||
| مقاله 15، دوره 80، شماره 5، آذر و دی 2025، صفحه 1209-1216 اصل مقاله (5 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.32598/ARI.80.5.3494 | ||
| نویسندگان | ||
| Salma Awad Taghyan* 1؛ Elham Fathy Mahmoud2؛ Rasha Mohamed Taha2؛ Mohamed Shamel1 | ||
| 1Department of Oral Biology, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt. | ||
| 2Department of Oral Biology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt. | ||
| چکیده | ||
| Introduction: Acrylamide (AA) is a chemical compound that poses a major public health concern. This study aimed to evaluate the protective effect of Nigella sativa (NS) oil against AA- induced toxicity on the submandibular salivary glands (SMGs) of Albino rats. Materials & Methods: Thirty male albino rats weighing 150–200 g were equally and randomly divided into some groups: Control group received normal saline vehicle daily via oral gavage for 30 days, AA group received 15 mg/kg body weight of AA dissolved in 0.2 mL saline solution daily via oral gavage for 30 days. NS group received 15 mg/kg body weight (bw) of AA combined with 1 mL/kg bw of NS oil daily via oral gavage for 30 days. At the end of the experiment, rats were euthanized, and SMGs were dissected for histological evaluation, including hematoxylin and eosin staining (H&E) and immunohistochemistry for inducible nitric oxide synthase (iNOS), as well as analysis for heme oxygenase-1 (HO-1) expression using real-time polymerase chain reaction (RT-qPCR). Results: The acinar and ductal cells of SMG of the AA group showed signs of degeneration and toxicity in the form of ill-defined outlines, pyknotic and crescent-shaped nuclei with different-sized cytoplasmic vacuolations. These changes were statistically significant with increased iNOS immunoexpression and HO-1 gene expression (P<0.0001). Administration of NS alleviated the toxic effect, downregulating both iNOS and HO-1 gene expression. The study revealed a significant cytotoxic effect of AA on SMGs of albino rats (P<0.05), presumably by the generation of oxidative stresses and mitochondrial dysfunction. Conclusion: NS effectively mitigated these toxic effects, suggesting its potential as a natural antioxidant. | ||
| کلیدواژهها | ||
| Salivary glands؛ Acrylamide؛ Nigella sativa؛ Oxidative stress | ||
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آمار تعداد مشاهده مقاله: 275 تعداد دریافت فایل اصل مقاله: 157 |
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