اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,036
تعداد مقالات33,984
تعداد مشاهده مقاله45,763,943
تعداد دریافت فایل اصل مقاله77,188,456
Maghami, Masoumeh, lotfi, Mohsen, Sotoodehnejadnematalahi, Fattah, Mohammadi, Ashraf. (1404). Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell. سامانه مدیریت نشریات علمی, 80(3), 759-768. doi: 10.32592/ARI.2025.80.3.759
Masoumeh Maghami; Mohsen lotfi; Fattah Sotoodehnejadnematalahi; Ashraf Mohammadi. "Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell". سامانه مدیریت نشریات علمی, 80, 3, 1404, 759-768. doi: 10.32592/ARI.2025.80.3.759
Maghami, Masoumeh, lotfi, Mohsen, Sotoodehnejadnematalahi, Fattah, Mohammadi, Ashraf. (1404). 'Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell', سامانه مدیریت نشریات علمی, 80(3), pp. 759-768. doi: 10.32592/ARI.2025.80.3.759
Maghami, Masoumeh, lotfi, Mohsen, Sotoodehnejadnematalahi, Fattah, Mohammadi, Ashraf. Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell. سامانه مدیریت نشریات علمی, 1404; 80(3): 759-768. doi: 10.32592/ARI.2025.80.3.759

Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell

Archives of Razi Institute
مقاله 26، دوره 80، شماره 3، مرداد و شهریور 2025، صفحه 759-768    اصل مقاله (584.22 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.3.759
نویسندگان
Masoumeh Maghami1؛ Mohsen lotfi* 2؛ Fattah Sotoodehnejadnematalahi3؛ Ashraf Mohammadi4
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran
3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran-Iran
4Department of Human Viral Vaccines, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده
RBK cell line derived from primary bovine kidney cell was established and introduced by Razi Vaccine and Serum Research Institute. RBK cell line has been turned into continuous cell line through serial passaging of primary cell. On the basis of the experiments conducted, RBK cell line demonstrates high sensitivity to viruses. As cell line identification is crucial to confirmation of the experimental results, and can attest to the validity and credibility of such findings. Misidentification of the cell line, misdiagnosis of its contamination or lack thereof, can lead to unreliable results as well as waste of resources. It is important to forecast the value of a cell line as it can determine its origin. The more the explanation of the origin, the more valuable the cell line. In addition, contamination is a serious concern in cell culture experiments as it can affect the outcomes, and result in false +/- faults. Therefore, based on standard routines, it is necessary to establish that the cell line is free contamination.
As for the purposes of identification, verification and authentication of a cell line, various methods must be applied simultaneously to a particular cell line; so, in this study, molecular tests such as PCR, PCR-RFLP and real time PCR were used to determine the degree of certainty in identification and purification of RBK cell line; The present study provides RBK cell line authentication; In addition, the results demonstrate the RBK is free of adventitious agents such as Bovine Leukemia Virus (BLV) and Bovine Viral Diarrhea (BVDV). In consideration of the RBK cell line capability to unlimited proliferation, stability and sensitivity to viruses, the present study introduces this cell line as a reliable tool for viral research, which can contribute to numerous important scientific advances.
کلیدواژه‌ها
cell line؛ PCR؛ PCR-RFLP؛ real time؛ RBK
عنوان مقاله [English]
بررسی و تعیین هویت سلول لاین RBK مشتق شده از سلول کلیه گاو
چکیده [English]
سلول لاین RBK در موسسه تحقیقات واکسن و سرم سازی رازی تهیه و معرفی گردیده است و نسبت به برخی ویروسها حساسیت قابل توجهی دارد. این سلول لاین از سلولهای پرایمری کلیه گاو مشتق گردیده است که با پاساژهای متوالی به فرم پیوسته در آمده است. برطبق آزمایشات انجام گرفته، سلول لاین RBK دارای حساسیت مناسب و قابل توجهی نسبت به ویروس ها می باشد. با در نظر گرفتن اینکه، برای تعیین هویت و صحت یک لاین سلولی و معتبر سازی آن باید روشهای مختلف همزمان در مورد یک سلول لاین استفاده شود؛ از اینرو در این مطالعه جهت بررسی و اطمینان از هویت و خلوص سلول لاین RBK از تستهای مولکولی مانند PCR و RFLP-PCR و real time PCR استفاده گردید. با استناد به نتایج بدست آمده از تستهای DNA finger printing و RFLP-PCR هویت و عدم آلودگی سلول لاین RBK به سلولهای سایر گونه ها اثبات گردید. بعلاوه نتایج تست Real-time PCR حاکی از عدم آلودگی سلول نسبت به ویروس های دامی BLV و BVDV را نشان داد. مطالعه حاضر با اطمینان از هویت و خلوص سلول لاین RBK ، این سلول لاین را بعنوان یک سلول قابل اطمینان برای تحقیقات ویروسی پیشنهاد میدهد.
کلیدواژه‌ها [English]
سلول لاین, PCR, real time, PCR-RFLP, RBK
مراجع
  1. Ambrose CT. The Tissue Culture Laboratory of Dr. George Otto Gey 60 yrs ago as recalled by a former student. In Vitro Cellular & Developmental Biology-Animal. 2017;53(5):467-73.
  2. Chen X, Qian W, Song Z, Li Q-X, Guo S. Authentication, characterization and contamination detection of cell lines, xenografts and organoids by barcode deep NGS sequencing. NAR Genomics and Bioinformatics. 2020;2(3):lqaa060.
  3. Maghami M, Lotfi M, Nematalahi FS, Mohammadi A. Characterization of Razi Bovine Kidney (RBK) Cell Line as a Sensitive Cell to Bovine Herpesvirus-1 (BoHV-1). Archives of Razi Institute. 2023;78(5):1528.
  4. Masoudi S. Evaluating of Iran Razi Khedmati Bovine Kidney (IRKHBK) Cell Line for Isolation and‎ Propagation of Viruses. Iranian Journal of Virology. 2014;8(4):20-4.
  5. Muhsen M, Aoki H, Ikeda H, Fukusho A. Biological properties of bovine viral diarrhea virus quasispecies detected in the RK13 cell line. Archives of virology. 2013;158(4):753-63.
  6. Hajia M, Mahmoodzadeh A, Morovati H. Use of Razi Bovine Kidney Cell Line for Proliferation of Pneumocystis Carinii. Iranian Journal of Pathology. 2008;3(4):197-202.
  7. Cooper JK, Sykes G, King S, Cottrill K, Ivanova NV, Hanner R, et al. Species identification in cell culture: a two-pronged molecular approach. In Vitro Cellular & Developmental Biology-Animal. 2007;43(10):344-51.
  8. Bravi CM, Lirón JP, Mirol PM, Ripoli MV, Peral-García P, Giovambattista G. A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene. Legal Medicine. 2004;6(4):246-51.
  9. Ridpath JF, Bolin SR. Differentiation of types 1a, 1b and 2 bovine viral diarrhoea virus (BVDV) by PCR. Molecular and cellular probes. 1998;12(2):101-6.
  10. Oie A. Manual of diagnostic tests and vaccines for terrestrial animals. Office international des epizooties, Paris, France. 2008;2002:1092-106.
  11. Eggleston H, Njoya K, Anderson CE, Holm I, Eiglmeier K, Liang J, et al. Molecular characterization and genetic authentication assay for Anopheles ‘hemocyte-like’cell lines 4a-3A and 4a-3B. Parasites & Vectors. 2022;15(1):465.
  12. Hay R. Cell line preservation and characterization. Animal cell culture: a practical approach. 1992:104-35.
  13. Romano P, Manniello A, Aresu O, Armento M, Cesaro M, Parodi B. Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines. Nucleic acids research. 2009;37(suppl_1):D925-D32.
  14. Tong L, Corrigan A, Kumar NR, Hallbrook K, Orme J, Wang Y, et al. An automated cell line authentication method for AstraZeneca global cell bank using deep neural networks on brightfield images. Scientific reports. 2022;12(1):7894.
  15. Halton DM, Peterson WD, Hukku B. Cell culture quality control by rapid isoenzymatic characterization. In vitro. 1983;19(1):16-24.
  16. Steube KG, Grunicke D, Drexler HG. Isoenzyme analysis as a rapid method for the examination of the species identity of cell cultures. In Vitro Cellular & Developmental Biology-Animal. 1995;31(2):115-9.
  17. Cabrera C, Cobo F, Nieto A, Cortés J, Montes R, Catalina P, et al. Identity tests: determination of cell line cross-contamination. Cytotechnology. 2006;51(2):45-50.
  18. Rojas A. The imperative authentication of cell lines. Antimicrobial agents and chemotherapy. 2017;61(11):10.1128/aac. 01823-17.
  19. PD H, editor Barcoding animal life: Cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci; 2003.
  20. Ferreira KM, e Silva OL, Arias MC, Del Lama MA. Cytochrome-b variation in Apis mellifera samples and its association with COI–COII patterns. Genetica. 2009;135(2):149-55.
  21. Rokas A, Williams BL, King N, Carroll SB. Genome-scale approaches to resolving incongruence in molecular phylogenies. Nature. 2003;425(6960):798-804.
  22. Marx V. Cell-line authentication demystified. Nature methods. 2014;11(5):483-8.
  23. Das A, Xu L, Jia W. Development of conventional and real time PCR assays for rapid species authentication of mammalian cell lines commonly used in veterinary diagnostic laboratories. Research in Veterinary Science. 2019;126:170-7.
  24. Corral-Vázquez C, Aguilar-Quesada R, Catalina P, Lucena-Aguilar G, Ligero G, Miranda B, et al. Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking. Cell and tissue banking. 2017;18(2):271-80.
  25. Mariyappa D, Rusch DB, Han S, Luhur A, Overton D, Miller DF, et al. A novel transposable element-based authentication protocol for Drosophila cell lines. G3. 2022;12(2):jkab403.
  26. Pajurek M, Warenik-Bany M, Mikolajczyk S. Dioxin transfer simulation from feed to animal tissues and risk assessment. Chemosphere. 2023;313:137379.
  27. Lung O, Candlish R, Nebroski M, Kruckiewicz P, Buchanan C, Moniwa M. High-throughput sequencing for species authentication and contamination detection of 63 cell lines. Scientific Reports. 2021;11(1):21657.
آمار
تعداد مشاهده مقاله: 84
تعداد دریافت فایل اصل مقاله: 153


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب