| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,273 |
| تعداد مقالات | 36,170 |
| تعداد مشاهده مقاله | 55,727,907 |
| تعداد دریافت فایل اصل مقاله | 94,506,998 |
Molecular Characterization of Canine Parvovirus in Iran, 2023 | ||
| Archives of Razi Institute | ||
| مقاله 24، دوره 81، شماره 1، فروردین و اردیبهشت 2026، صفحه 213-222 اصل مقاله (632.39 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ARI.81.1.3354 | ||
| نویسندگان | ||
| Zahra Ziafati Kafi1؛ Soroush Sarmadi1؛ Shabnam Babazadeh2؛ Fahimeh Jamiri1؛ Alireza Bakhshi1؛ Aryan Abbassioun3؛ Omid Eghbali1؛ Arash Ghalyanchilangeroudi* 1 | ||
| 1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 3Department of Virology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran | ||
| چکیده | ||
| Introduction: Canine parvo virus 2 causes severe and often fatal gastroenteritis and myocarditis in dogs and puppies. Based on the VP2 gene, this virus is classified into 3 variants: CPV-2a, CPV-2b, and CPV-2c. Materials & Methods: In present study, 35 rectal swab samples were collected from dogs with clinical signs, including vomiting and diarrhea, and cases with positive results from the rapid test kit. Samples were screened with polymerase chain reaction (PCR) assay to detect the presence of the virus genome. According to the PCR results, all samples were positive. Results: Out of 35 cases, about 34% received at least one dose of vaccine, and almost 66% were not vaccinated at all. A rapid test was also performed for 34 cases; it was positive for 91% (31 cases) and negative for 9% (3 cases). Phylogenetic analysis of seven samples, which were submitted for sequencing, revealed that 6 of the present isolates (UT-CPV14 to UT-CPV18 and UT-CPV20) were clustered with CPV-2c isolates, and one (UT-CPV19) was clustered with CPV-2b sequences. Homology analysis indicated high similarity (100%) between isolates (UT-CPV14 to UT-CPV18 and UT-CPV20) and isolates K20172c-1, 12B, IZSSI_2021PA43108idAki, BJ001, and CPV-2c/Sull6/2017. UT-CPV19 showed 100% similarity with isolates 19R113-2, YANJI-2, and 15D184. In the present study, we also phylogenetically analyzed a commercial vaccine. Although the homology results indicated almost 98% similarity between the current isolates and the vaccine, the vaccine sequence did not cluster with any groups in the phylogenetic tree. Conclusion: These results highlight the importance of constantly monitoring antigenic changes of circulating strains and the efficacy of vaccines against them. | ||
| کلیدواژه‌ها | ||
| Canine parvovirus؛ Dog؛ Vaccine؛ Phylogeny؛ Iran | ||
| عنوان مقاله [English] | ||
| تعیین هویت مولکولی پاروویروس سگ سانان در ایران، 2023 | ||
| چکیده [English] | ||
| پاروویروس 2 سگ سانان، سبب گاستروانتریت و مایوکاردیت شدید و کشنده در بین سگ‌ها و توله سگ‌ها می‌‌شود. این ویروس بر اساس ژن VP2 به سه واریانت: CPV-2a, CPV-2b و CPV-2c طبقه بندی می‌‌گردد. در مطالعه‌ی حاضر تعداد 35 عدد نمونه سواب مقعدی از سگ‌های دارای علایم بالینی از جمله استفراغ و اسهال و موارد بالینی با نتیجه مثبت تست سریع جمع آوری شد. نمونه‌های جمع آوری شده از نظر وجود آلودگی با پاروویروس 2 سگ سانان با استفاده از تست PCR مورد بررسی قرار گرفتند. بر اساس نتایج تست PCR تمامی نمونه‌ها مثبت بودند. از بین 35 سگ واجد شرایط نمونه برداری 34% حداقل یک دز واکسن دریافت کرده و حدود 66% واکسینه نشده بودند. نتایج تست سریع 91% از موارد (31 مورد) مثبت و 9% موارد (3 مورد) منفی بودند. تجزیه و تحلیل فیلوژنی هفت نمونه که تعیین توالی شده بودند نشان داد که 6 نمونه (UT-CPV14 تا UT-CPV-18 و UT-CPV-20) در زیرگروه 2c قرار گرفته و یک نمونه (UT-CPV-19) در زیرگروه 2b قرار گرفت. تجزیه و تحلیل همولوژی شباهت بالایی (100%) بین جدایه‌های (UT-CPV14 تا UT-CPV18 و UT-CPV20) و جدایه‌های K20172c-1، 12B، IZSSI_2021PA43108idAki، BJ001 و CPV-2c/Sull6/2017 و جدایه UT-CPV19 با جدایه‌های 19R113-2، YANJI-2 و 15D184 شباهت 100% نشان داد. در مطالعه‌ی حاضر یک واکسن تجاری نیز مورد بررسی فیلوژنی قرار گرفت. اگرچه نتایج همولوژی بیانگر شباهتی معادل 98% بین توالی واکسن و جدایه‌های مطالعه‌ی حاضر بود، واکسن مورد مطالعه در هیچ دسته‌ای در درخت فیلوژنی قرار نگرفت. نتایج این مطالعه بیانگر اهمیت پایش مداوم تغییرات آنتی‌ژنی سویه‌های در گردش و تاثیر واکسن‌ها در برابر آن‌ها می‌باشد. | ||
| کلیدواژه‌ها [English] | ||
| پاروویروس سگ سانان, سگ, واکسن, فیلوژنی, ایران | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Ansari-Lari M, Oroji E. Knowledge, attitudes and practices of dog and cat owners toward zoonotic diseases in Shiraz, southern Iran. Prev Vet Med. 2023; 215:105926. [DOI:10.1016/j.prevetmed.2023.105926] [PMID]
Dodds WJ. Early life vaccination of companion animal pets. Vaccines. 2021; 9(2):92. [DOI:10.3390/vaccines9020092][PMID]
Tuteja D, Banu K, Mondal B. Canine parvovirology-A brief updated review on structural biology, occurrence, pathogenesis, clinical diagnosis, treatment and prevention. Comp Immunol Microbiol Infect Dis. 2022; 82:101765. [DOI:10.1016/j.cimid.2022.101765] [PMID]
Ghajari M, Pourtaghi H, Lotfi M. Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran. Iran J Vet Res. 2021; 22(4):347-51. [PMID]
Liu C, Gao J, Li H, Sun F, Liang H, Liu H, et al. Phylogenetic characteristics of canine parvovirus type 2c variant endemic in Shanghai, China. Viruses. 2021; 13(11):2257. [DOI:10.3390/v13112257][PMID]
Nikbakhat G, Shahram S, Mohyedidini S. Detection of a new canine parvovirus mutant in Iran. Iran J Vet Med. 2018; 12(1):1-7. [DOI:10.22059/ijvm.2018.231479.1004805]
Decaro N, Buonavoglia C, Barrs VR. Canine parvovirus vaccination and immunisation failures: Are we far from disease eradication? Vet Microbiol. 2020; 247:108760. [DOI:10.1016/j.vetmic.2020.108760][PMID]
Khatri R, Poonam MH, Minakshi PC. Epidemiology, pathogenesis, diagnosis and treatment of canine parvovirus disease in dogs: A mini review abstract. J Vet Sci Med Diagn. 2017; 6(3):2. [DOI:10.4172/2325-9590.1000233]
Hao X, Li Y, Xiao X, Chen B, Zhou P, Li S. The changes in canine parvovirus variants over the years. Int J Mol Sci. 2022; 23(19):11540. [DOI:10.3390/ijms231911540][PMID]
Qi S, Zhao J, Guo D, Sun D. A mini-review on the epidemiology of canine parvovirus in China. Front Vet Sci. 2020; 7:5. [DOI:10.3389/fvets.2020.00005][PMID]
Giraldo-Ramirez S, Rendon-Marin S, Ruiz-Saenz J. Phylogenetic, evolutionary and structural analysis of canine parvovirus (CPV-2) antigenic variants circulating in Colombia. Viruses. 2020; 12(5):500. [DOI:10.3390/v12050500][PMID]
Zaher KS, El-Dabae WH, El-Sebelgy MM, Aly NI, Salama ZT. Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt. Vet World. 2020; 13(2):326-33.[DOI:10.14202/vetworld.2020.326-333][PMID]
Clark NJ, Seddon JM, Kyaw-Tanner M, Al-Alawneh J, Harper G, McDonagh P, et al. Emergence of canine parvovirus subtype 2b (CPV-2b) infections in Australian dogs. Infect Genet Evol. 2018; 58:50-5. [DOI:10.1016/j.meegid.2017.12.013] [PMID]
Decaro N, Desario C, Addie DD, Martella V, Vieira MJ, Elia G, et al. Molecular epidemiology of canine parvovirus, Europe. Emerg Infect Dis. 2007; 13(8):1222. [Link]
Yip HYE, Peaston A, Woolford L, Khuu SJ, Wallace G, Kumar RS, et al. Diagnostic challenges in canine parvovirus 2c in vaccine failure cases. Viruses. 2020; 12(9):980. [DOI:10.3390/v12090980][PMID]
Altman KD, Kelman M, Ward MP. Are vaccine strain, type or administration protocol risk factors for canine parvovirus vaccine failure? Vet Microbiol. 2017; 210:8-16. [DOI:10.1016/j.vetmic.2017.08.019] [PMID]
Battilani M, Modugno F, Mira F, Purpari G, Di Bella S, Guercio A, et al. Molecular epidemiology of canine parvovirus type 2 in Italy from 1994 to 2017: Recurrence of the CPV-2b variant. BMC Vet Res. 2019; 15(1):393. [DOI:10.1186/s12917-019-2096-1][PMID]
Hematzadeh F, Jamshidi S. [First report of isolation of canine parvovirus in Iran (Persian)]. JVet Res. 2002; 57(2):33-5. [Link]
Askari Firoozjaii H, Jafari Shoorijeh S, Mohammadi A, Tamadon A. Characterization of Iranian isolates of canine parvovirus in fecal samples using polymerase chain reaction assay. Iran J Biotechnol. 2011; 9(1):63-8. [Link]
Faraji R, Mostafavi B, Sadeghi M, Decaro N, Vasinioti V, Desario C, et al. Genomic characterization and phylogenetic evolution of the canine parvoviruses in Iranian dogs, a nationwide study: CPV evolutionary analysis in Iran. Acta Trop. 2023; 244:106948. [DOI:10.1016/j.actatropica.2023.106948] [PMID]
Saei HD, Javadi S, Akbari S, Hadian N, Zarza E. Molecular characterization of canine parvovirus (CPV) antigenic variants from healthy and diarrheic dogs in Urmia region, Iran. Iran J Vet Med. 2017; 11(1):9-19. [Link]
Sinaclon Co. SinaPure OneViral nucleic acid extraction mini kit: Manufacturer’s instructions. Tehran (Iran): Sinaclon Co.; 2026.
Decaro N, Desario C, Miccolupo A, Campolo M, Parisi A, Martella V, et al. Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis. J Gen Virol. 2008; 89(Pt 9):2290-8. [DOI:10.1099/vir.0.2008/001503-0] [PMID]
Kwan E, Carrai M, Lanave G, Hill J, Parry K, Kelman M, et al. Analysis of canine parvoviruses circulating in Australia reveals predominance of variant 2b and identifies feline parvovirus‐like mutations in the capsid proteins. Transbound Emerg Dis. 2021; 68(2):656-66. [DOI:10.1111/tbed.13727] [PMID]
Amrani N, Desario C, Kadiri A, Cavalli A, Berrada J, Zro K, et al. Molecular epidemiology of canine parvovirus in Morocco. Infect Genet Evol. 2016; 41:201-6. [DOI:10.1016/j.meegid.2016.04.005] [PMID]
National Center for Biotechnology Information (NCBI). BLAST: Basic local alignment search tool [Internet]. 2026 [Updated 15 April 2026]. Available from: [Link]
Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016; 33(7):1870-4. [DOI:10.1093/molbev/msw054][PMID]
Yi L, Tong M, Cheng Y, Song W, Cheng S. Phylogenetic Analysis of Canine Parvovirus VP 2 Gene in China. Transbound Emerg Dis. 2016; 63(2):e262-9. [DOI:10.1111/tbed.12268] [PMID]
Nguyen Manh T, Piewbang C, Rungsipipat A, Techangamsuwan S. Molecular and phylogenetic analysis of Vietnamese canine parvovirus 2C originated from dogs reveals a new Asia‐IV clade. Transbound Emerg Dis. 2021; 68(3):1445-53. [DOI:10.1111/tbed.13811] [PMID]
Zhao Y, Lin Y, Zeng X, Lu C, Hou J. Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing, China. Virol J. 2013; 10:272. [DOI:10.1186/1743-422X-10-272][PMID]
Decaro N, Desario C, Parisi A, Martella V, Lorusso A, Miccolupo A, et al. Genetic analysis of canine parvovirus type 2c. Virology. 2009; 385(1):5-10. [DOI:10.1016/j.virol.2008.12.016] [PMID]
Zienius D, Lelešius R, Kavaliauskis H, Stankevičius A, Šalomskas A. Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples. Pol J Vet Sci. 2016; 19(1):187-96. [DOI:10.1515/pjvs-2016-0023] [PMID]
Abedi N, Staji H, Shahroozian E. Frequency of canine parvovirus type-2 variants by PCR in enteric and healthy dogs and genotyping of variants by restriction enzyme (RE) mapping by DdeI endonuclease based on partial VP-2 gene sequence. Molecular Genetics, Mol Genet Microbiol Virol. 2018; 33:151-5. [DOI:10.3103/S0891416818020027]
Kelman M, Barrs VR, Norris JM, Ward MP. Canine parvovirus prevention and prevalence: Veterinarian perceptions and behaviors. Prev Vet Med. 2020; 174:104817. [DOI:10.1016/j.prevetmed.2019.104817] [PMID]
Wilson S, Illambas J, Siedek E, Stirling C, Thomas A, Plevová E, et al. Vaccination of dogs with canine parvovirus type 2b (CPV-2b) induces neutralising antibody responses to CPV-2a and CPV-2c. Vaccine. 2014; 32(42):5420-4. [DOI:10.1016/j.vaccine.2014.07.102] [PMID]
Miranda C, Thompson G. Canine parvovirus in vaccinated dogs: A field study. Vet Rec. 2016; 178(16):397. [DOI:10.1136/vr.103508] [PMID]
Dall’Ara P, Lauzi S, Filipe J, Caseri R, Beccaglia M, Desario C, et al. Discrepancy between in-clinic and haemagglutination-inhibition tests in detecting maternally-derived antibodies against canine parvovirus in puppies. Front Vet Sci. 2021; 8:630809. [DOI:10.3389/fvets.2021.630809][PMID]
Singh P, Kaur G, Chandra M, Dwivedi PN. Prevalence and molecular characterization of canine parvovirus. Vet World. 2021; 14(3):603-6. [DOI:10.14202/vetworld.2021.603-606][PMID]
Sayed-Ahmed MZ, Elbaz E, Younis E, Khodier M. Canine parvovirus infection in dogs: Prevalence and associated risk factors in Egypt. World Vet J. 2020; 10(4):571-7. [DOI:10.54203/scil.2020.wvj68]
Tagorti G. Prevalence of canine parvovirus infection in Grand Tunis, Tunisia. J Adv Vet Anim Res. 2018; 5(1):93-7. [DOI:10.5455/javar.2018.e251]
Behera M, Panda SK, Sahoo PK, Acharya AP, Patra RC, Das S, et al. Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India. Vet World. 2015; 8(1):33-7. [DOI:10.14202/vetworld.2015.33-37][PMID]
Tinky SS, Ambily R, Nair SR, Mini M. Utility of a rapid immunochromatographic strip test in detecting canine parvovirus infection compared with polymerase chain reaction. Vet World. 2015; 8(4):523-6. [DOI:10.14202/vetworld.2015.523-526][PMID]
Mohyedini S, Jamshidi S, Rafati S, Nikbakht GR, Malmasi A, Taslimi Y, et al. Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus. Iran J Vet Med. 2013; 7(1):57-61. [Link] | ||
|
آمار تعداد مشاهده مقاله: 1,256 تعداد دریافت فایل اصل مقاله: 868 |
||
