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Mohamadzadeh, Tahereh, Arsalani, Saeideh, Hafezi, Fatemeh, Sadjjadi, Seyed Mahmoud, Heidari, Soudabeh, Sharifzadeh, Parviz, Darabi, Enayat. (1404). Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method. سامانه مدیریت نشریات علمی, 80(4), 1023-1028. doi: 10.66224/ARI.80.4.1023
Tahereh Mohamadzadeh; Saeideh Arsalani; Fatemeh Hafezi; Seyed Mahmoud Sadjjadi; Soudabeh Heidari; Parviz Sharifzadeh; Enayat Darabi. "Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method". سامانه مدیریت نشریات علمی, 80, 4, 1404, 1023-1028. doi: 10.66224/ARI.80.4.1023
Mohamadzadeh, Tahereh, Arsalani, Saeideh, Hafezi, Fatemeh, Sadjjadi, Seyed Mahmoud, Heidari, Soudabeh, Sharifzadeh, Parviz, Darabi, Enayat. (1404). 'Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method', سامانه مدیریت نشریات علمی, 80(4), pp. 1023-1028. doi: 10.66224/ARI.80.4.1023
Mohamadzadeh, Tahereh, Arsalani, Saeideh, Hafezi, Fatemeh, Sadjjadi, Seyed Mahmoud, Heidari, Soudabeh, Sharifzadeh, Parviz, Darabi, Enayat. Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method. سامانه مدیریت نشریات علمی, 1404; 80(4): 1023-1028. doi: 10.66224/ARI.80.4.1023

Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method

Archives of Razi Institute
مقاله 24، دوره 80، شماره 4، مهر و آبان 2025، صفحه 1023-1028    اصل مقاله (503.38 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.66224/ARI.80.4.1023
نویسندگان
Tahereh Mohamadzadeh1، 2؛ Saeideh Arsalani3؛ Fatemeh Hafezi4؛ Seyed Mahmoud Sadjjadi5؛ Soudabeh Heidari3؛ Parviz Sharifzadeh6؛ Enayat Darabi* 7
1Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Health Research center, Life style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4Department of Parasitology and Mycology, School of Medicine, Semnan University of Medical Sciences, Semnan,
5Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
6Department of Pathology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
7Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Abstract
Cystic echinococcosis (CE) is a common zoonotic infection caused by the metacestode form of Echinococcus granulosus. The disease is found worldwide, and Iran is considered an endemic area. Depending on the affected organ, the disease has different clinical signs and symptoms. Humans are regarded as dead-end intermediate hosts. CE cysts commonly develop in the liver and lungs, while they are less frequently found in other organs. Timely diagnosis of this disease is essential in humans. Diagnosis relies on imaging methods (X-ray, ultrasound, MRI, and CT scan) complemented by serological testing. The present study used the indirect ELISA method to compare the efficacy of in-house-produced antigen B (AgB) and a domestic commercial kit. Hydatid cyst fluid (HCF) was extracted from the sheep liver infected with cystic echinococcosis cyst followed by preparation of AgB. It was used in the indirect ELISA test. A total of 142 sera consisting of 36 patients with pathologically confirmed CE, 46 cases with other parasitic diseases, and 60 healthy cases were examined using in-house AgB and a domestic commercial ELISA kit. Our findings revealed the sensitivity and specificity of the indirect ELISA using in-house AgB to be 86.11% and 95.28% respectively. The commercial kit demonstrated a sensitivity of 77.78% and a specificity of 99.06%. Although both in-house-produced AgB and commercial kit showed considerable and relatively similar diagnostic efficiency, the study results showed a higher sensitivity and validity of AgB compared with the commercial ELISA kit.
Keywords: Cystic echinococcosis, Diagnosis, AgB, ELISA
Keywords: Cystic echinococcosis, Diagnosis, AgB, ELISA
کلیدواژه‌ها
Cystic echinococcosis؛ Diagnosis؛ AgB؛ ELISA
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مراجع
  1. Thompson R. The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008; 119(4):439–46.
  2. Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev. 2004; 17(1):107–35.
  3. Sadjjadi SM. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int. 2006; 55:S197–202.
  4. Borhani M, Fathi S, Darabi E, Jalousian F, Simsek S, Ahmed H, et al. Echinococcoses in Iran, Turkey, and Pakistan: old diseases in the new millennium. Clin Microbiol Rev. 2021; 34(3):e00290-20.
  5. Fasihi Harandi M, Budke CM, Rostami S. The monetary burden of cystic echinococcosis in Iran. PLoS Negl Trop Dis. 2012; 6 (11):e1915.
  6. Parandin F, Heydarpour F, Mohebali M, Hanafi-Bojd AA, Sari AA, Zeynali M, et al. Estimation of Burden of Cystic Echinococcosis in Iran Using Disability Adjusted Life Years (DALYs) in 2018. Iran J Public Health. 2021; 50 (11):2302.
  7. Eckert J, Gemmell M, Meslin FX, Pawlowski Z, World Health Organization. WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. World Organisation for Animal Health; 2001.
  8. Torgerson PR, Deplazes P. Echinococcosis: diagnosis and diagnostic interpretation in population studies. Trends Parasitol. 2009; 25(4):164–70.
  9. WHO Informal Working Group. International classification of ultrasound images in cystic echinococcosis for application in clinical and field epidemiological settings. Acta Trop. 2003; 85(2):253–61.
  10. Darabi E, Motevaseli E, Mohebali M, Rokni MB, Khorramizadeh MR, Zahabiun F, et al. Evaluation of a novel Echinococcus granulosus recombinant fusion B-EpC1 antigen for the diagnosis of human cystic echinococcosis using indirect ELISA in comparison with a commercial diagnostic ELISA kit. Exp Parasitol. 2022; 240:108339.
  11. Mohammadzadeh T, Sako Y, Sadjjadi SM, Sarkari B, Ito A. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371–5.
  12. Jalousian F, Hosseini SH, Fathi S, Shirani D, Aghaei S, Kordafshari S. Comparative assessment of rEPC1 antigen and copro-antigen for diagnosis of echinococcosis in dogs. Iran J Vet Med. 2017; 11(3):217-225.
  13. Farhoudi R, Hosseini SH, Siavashi MR, Abolhassani M, Shayan P, Nejadmoghadam A. Identification and purification of a specific and immunogenic antigen of the laminated layer of the hydatid cyst and production of an antigen-specific monoclonal antibody. Int J Vet Res. 2010; 4(4): 237-43
  14. Lightowlers MW, Liu D, Haralambous A, Rickard MD. Subunit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus. Mol Biochem Parasitol. 1989; 37(2):171–82.
  15. Oriol R, Williams J, Pérez E, Oriol C. Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg.1971; 20(4):569–74.
  16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72(1–2):248–54.
  17. Schoonjans F, Zalata A, Depuydt CE, Comhaire FH. MedCalc: a new computer program for medical statistics. Comput Methods Programs Biomed. 1995; 48(3):257–62.
  18. Zhang W, Wen H, Li J, Lin R, McManus DP. Immunology and immunodiagnosis of cystic echinococcosis: an update. Clin Dev Immunol. 2012; 2012.
  19. Virginio V, Hernandez A, Rott M, Monteiro K, Zandonai A, Nieto A, et al. A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clin Exp Immunol. 2003; 132(2):309–15.
  20. Schantz PM, Shanks D, Wilson M. Serologic cross-reactions with sera from patients with echinococcosis and cysticercosis. Am J Trop Med Hyg. 1980; 29(4):609–12.
  21. Barbieri M, Fernández V, González G, Luaces VM, Nieto A. Diagnostic evaluation of a synthetic peptide derived from a novel antigen B subunit as related to other available peptides and native antigens used for serology of cystic hydatidosis. Parasite Immunol. 1998; 20(2):51–61.
  22. Lorenzo C, Ferreira HB, Monteiro KM, Rosenzvit M, Kamenetzky L, García HH, et al. Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study. J Clin Microbiol. 2005; 43(6):2764–70.
  23. Thompson RCA, Deplazes P, Lymbery AJ, Editors. Echinococcus and Echinococcosis, Part A. Volume 95, Advances in Parasitology 95. Academic Press; 2017. 525 pages,
  24. Reiterová, K., Auer, H., Altintaś, N., & Yolasigmaz, A. Evaluation of purified antigen fraction in the immunodiagnosis of cystic echinococcosis. Parasit res. 2014 ;( 113):2861–7.
  25. Sadjjadi FS, Mohammadzadeh T, Jafari SH, Sharifi Y, Deilami HN, Hafezi F, Sadjjadi SM. Comparison of Native Hydatid Cyst Fluid (HCF), Lyophilized HCF, Antigen B (AgB) and Lyophilized AgB (LAgB) Originated from Echinococcus granulosus Sensu Stricto for Sero- Diagnosis of Active, Transitional and Inactive Human Liver Cystic Echinococcosis. Iran J Public Health. 2023; 52(8):1764-72.
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