Investigating the role of priming with humic acid on modulating the effect of salinity stress on the germination and growth indices of cucumber (Cucumis sativus L. cv. Saba hybrid) seed | ||
| علوم و فناوری بذر ایران | ||
| Article 9, Volume 14, Issue 3 - Serial Number 39, September 2025, Pages 43-60 PDF (1.32 M) | ||
| Document Type: Original Article | ||
| DOI: 10.22092/ijsst.2024.364534.1511 | ||
| Authors | ||
| Reza Rezvani* 1; Behnam Kamkar2; Zeinab Jabbari Badkhor3 | ||
| 1Ph.D. Student in Crop Physiology, Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 2Professor, Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 3M.Sc. Graduated in Crop Physiology, Department of Plant Production and Genetic, Shirvan Faculty of Agriculture, University of Bojnord, Bojnord, Iran. | ||
| Abstract | ||
| To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination characteristics and growth parameters of cucumber (Cucumis sativus) under salt stress. Two experiments, one in the laboratory and the other in a greenhouse, were carried out using a factorial design based on a completely randomized design with three replications. The experimental factors included humic acid priming at four concentrations (0, 50, 100, and 200 mg/l-1) and salinity at five levels (0, 25, 50, 100, and 150 mM NaCl). Results indicated that humic acid exerted a positive impact on various germination and growth attributes of cucumber, including germination percentage, germination rate, seedling length, dry weight, vigor index, and chlorophyll a, b, and carotenoid under salt-stressed conditions compared to the control. The treatment with 200 mmol/l humic acid and no salt exhibited the highest germination rate (87%) and seedling length (12.66 cm). The interaction between salinity and humic acid revealed that humic acid concentrations exceeding 100 mg/l-1 could alleviate the adverse effects of salinity stress on total chlorophyll. In conclusion, the cost-effective and easily applicable method of priming cucumber seeds with humic acid presents a potential strategy to mitigate the impacts of salinity stress in regions with saline water or soil. | ||
| Keywords | ||
| Pretreatment; Sodium Chloride (NaCl); Germination percentage and speed; Growth characteristics; Chlorophyll | ||
| References | ||
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Azarmi, R., Tabatabaei, J., & Chaparzadeh, N. (2018). Effects of Mg²⁺ and root zone temperature on growth, yield, and physiological properties of greenhouse cucumber (Cucumis sativus L.) in hydroponics. Journal of Soil and Plant Interactions Isfahan University of Technology, 9(2), 11-22. https://doi.org/10.29252/ejgcst.9.2.11 [In Persian] Alizadeh Ahmadabadi, A., & Khorasaninejad, S. (2016). The effect of humic acid pretreatment on germination of purple cornflower (Echinacea purpurea) under drought and salinity conditions. Journal of Arid Biome, 6(2), 97-107. [In Persian] Abdulbaki, A. A., & Anderson, J. D. (1973). Vigour determination in soybean by multiple criteria. Journal of Crop Sciences, 13, 630-633. https://doi.org/10.2135/cropsci1973. Amiri, M. B., Rezvani Moghaddam, P., Ehyai, H. R., Fallahi, J., & Aghhavani Shajari, M. (2010). Effect of osmotic and salinity stresses on germination and seedling growth indices of artichoke (Cynara scolymus) and purple coneflower (Echinacea purpurea). Journal of Environmental Stresses in Crop Sciences, 3(2), 165-176. https://doi.org/10.22077/escs.2011.92 [In Persian] Ashraf, M., & Waheed, A. (1990). Screening of local exotic lentil (Lens culinaris Medik) for salt tolerance at two growth stages. Journal of Plant and Soil, 128, 167-176. Ashraf, M., Athar, H. R., Harris, P. J. C., & Kwon, T. R. (2008). Some prospective strategies for improving crop salt tolerance. Journal of Advances in Agronomy, 97, 45–110. https://doi.org/10.1016/S0065-2113(07)00002-8 [In Persian] Azarnivand, H., & Jafarian, Z. (2005). Effect of salinity on seed germination of Agropyron desertorum and Agropyron cristatum. Journal of Desert, 8, 52-61. Bayat, H., Naseri Moghaddam, A., & Aminifard, M. (2020). Allelopathic effects of narcissus (Narcissus tazetta L.) extract on germination, growth, and physiological characteristics of couch grass (Agropyron repens) and wild oat (Avena fatua). Journal of Seed Sciences Research, 6(4), 457-469. https://doi.org/10.22124/JMS.2020.3925 [In Persian] Bohnert, H. J., Nelson, D. E., & Jense, R. G. (1995). Adaptation to environmental stresses. Journal of Plant Cell, 7(7), 1099–1111. https://doi.org/10.1105/tpc.7.7.1099 Ebrahimi, M., & Miri Karbasak, E. (2015). Investigation of the effect of humic acid on germination, seedling growth, and photosynthesis pigments of medicinal plant Isabgol (Plantago ovata Forssk). Journal of Seed Sciences and Research, 3(3), 35-46. https://doi.org/20.1001.1.24763780.1395.3.3.4.2 [In Persian] Egert, M., & Tevini, M. (2002). Influence of drought on some physiological parameters symptomatic of oxidative stress in leaves of chives (Allium schoenoprasum). Journal of Environmental and Experimental Botany, 48(1), 43–49. https://doi.org/10.1016/S0098-8472(02)00008-4 Eisa Salwa, A. I. (2011). Effect of amendments, humic and amino acids on soil fertility, yields, and seed quality of peanut and sesame on sandy soils. Research Journal of Agriculture and Biological Sciences, 7(1), 115-125. El Mohamedy, R. S. R., & Ahmed, M. A. (2009). Effect of biofertilizers and humic acid on control of dry root disease and improvement of yield quality of mandarin (Citrus reticulata Blanco). Research Journal of Agriculture and Biological Sciences, 5(2), 127-137. Food and Agricultural Organization (FAO). (2014). Retrieved September 9, 2015. Ghabbour, E. A., & Davies, G. (2001). Humic substances: Structures, models, and functions. Royal Society of Chemistry (Great Britain). Ghasemi Arian, A., Ghorbani, R., Nasripour Yazdi, M., & Mesdaghi, M. (2016). Effect of temperature on seed germination characteristics of Dorema ammoniacum. Journal of Plant Research (Iranian Journal of Biology), 29(3), 686-693. https://doi.org/20.1001.1.23832592.1395.29.3.20.6 [In Persian] Gulser, F., Sonmez, F., & Boysan, S. (2010). Effects of calcium nitrate and humic acid on pepper seedling growth under saline conditions. Journal of Environmental Biology, 31(5), 873-876. Hampson, C. R., & Simpson, D. M. (1990). Effect of temperature, salt, and osmotic potential on early growth of wheat. II. Early seedling growth. Canadian Journal of Botany, 68(1), 524-528. https://doi.org/10.1139/b90-073 Ikic, I., Maricevic, M., Tomasovic, S., Gunjaca, J., Sarcevic, Z., & Arcevic, H. (2012). The effect of germination temperature on seed dormancy in winter wheats. Journal of Euphytica, 188, 25-34. https://doi.org/10.1007/s10681-012-0735-8 Jamil, M., Lee, D. B., Jung, K. Y., Ashraf, M., Lee, S. H., & Rha, E. S. (2006). Effect of salt (NaCl) stress on germination and early seedling growth of four vegetable species. Journal of Central European Agriculture, 7(2), 273-281. Kafi, M., Nezami, A., Hosseyni, H., & Masumi, A. (2005). The physiological effects of stress caused by PEG on germination varieties of lentils. Journal of Agricultural Research of Iran, 3(1), 69-80. https://doi.org/10.22067/GSC.V3I1.1293 [In Persian] Karami, L., Hedayat, M., & Farahbakhsh, S. (2020). Effect of salicylic acid priming on seed germination and morphophysiological and biochemical characteristics of tomato seedlings (Lycopersicon esculentum). Journal of Seed Research, 7(1), 165-179. https://doi.org/10.29252/yujs.7.1.165 [In Persian] Maguire, J. D. (1962). Speed of germination aid in selection and evaluation for seedling emergence and vigor. Journal of Crop Science, 2(2), 176–177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x Manchanda, G., & Grag, N. (2008). Salinity and its effects on the functional biology of legumes. Acta Physiologiae Plantarum, 30, 595–618. https://doi.org/10.1007/s11738-008-0173-3 Miller, T. R., & Chapman, S. R. (1978). Germination responses of three forage grasses to different concentrations of six salts. Journal of Range Management, 31(2), 123-124. https://doi.org/10.2307/3897659 Naderi, S., Pizzeghello, D., Muscolo, A., & Vianello, A. (2002). Physiological effects of humic substances on higher plants. Journal of Soil Biology and Biochemistry, 34(11), 1527–1536. https://doi.org/10.1016/S0038-0717(02)00174-8 [In Persian] Nakhyeinejad, B., & Moosavi, S. G. R. (2015). Effect of irrigation interval, humic acid, and sulfur fertilizer on morphological and yield traits of fenugreek (Trigonella foenum-gracum L.). Journal of Plant Ecophysiology, 9(30), 40-51. https://doi.org/20.1001.1.20085958.1396.9.30.4.8 [In Persian] Padem, H. Ocal, A., & Alan, R. (1999). Effect of humic acid added foliar fertilizer on quality and nutrient content of eggplant and pepper seedlings. Journal of Acta Horticulturae, 491, 241-245. https://doi.org/10.17660/ActaHortic.1999.491.35 Piccolo, A., Celano, G., & Pietramellara, G. (1993). Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings (Lactuca sativa and Lycopersicum esculentum). Journal of Biology and Fertility of Soils, 16, 11-15. https://doi.org/10.1007/BF00336508 Raeisinejad Doboneh, R., & Yazdanpanah, N. (2019). Effect of different levels of irrigation and salinity on yield of greenhouse Cucumis sativus. Iranian Journal of Irrigation and Drainage, 13(5), 1471-1480. [In Persian] Samavat, S., & Malakuti, M. (2006). Important use of organic acids (humic and fulvic) for increasing quantity and quality of agricultural production. Water and Soil Researchers Technical, 463, 1-13. Sabzevari, S., & Khazaei, H. R. (2009). The effect of foliar application with humic acid on growth, yield, and yield components of wheat (Triticum aestivum L.). Journal of Agroecology, 1(2), 53-63. https://doi.org/10.22067/JAG.V1I2.2686 [In Persian] Sabzevari, S., Khazaei, H. R., & Kafi, M. (2010). The study of the effect of humic acid on the germination of four varieties of autumn wheat (Sayons and Sabalan) and spring wheat (Chamran and Pishtaz). Journal of Field Crops Research, 8(3), 473-480. https://doi.org/10.22067/GSC.V8I3.7765 [In Persian] Sabzevari, S., Khazaei, H. R., & Kafi, M. (2009). Effect of humic acid on root and shoot growth of two wheat cultivars (Triticum aestivum L.). Journal of Water and Soil, 23(2), 87-94. https://doi.org/10.22067/JSW.V0I0.1731 [In Persian] Safarnejad, A., Salami, M. R., & Hamidi, H. (2007). Morphological characteristics of Plantago psyllium and Plantago ovata against salt stress. Journal of Construction Research, 75, 152-160. [In Persian] Salehzadeh, H., Izadkhah Shishvan, M., & Chiyasi, M. (2009). Effect of seed priming on germination and seedling growth of wheat (Triticum aestivum L.). Journal of Biological Sciences, 4(5), 629-631. https://doi.org/rjbsci.2009.629.631 Tejada, M., & Gonzalez, J. (2003). Influence of foliar fertilization with amino acids and humic acids on productivity and quality of asparagus. Journal of Biological Agriculture and Horticulture, 21(3), 277-291. https://doi.org/10.1080/01448765.2003.9755270 [In Persian] Turkman, O., Demir, S., Sensoy, S., & Dursun, A. (2005). Effect of arbuscular mycorrhizal fungus and humic acid on the seedling development and nutrient content of pepper grown under saline soil conditions. Journal of Biological Sciences, 5, 565-574. https://doi.org/10.3923/jbs.2005.568.574 [In Persian] Yazdani Bioki, R., & Rezvani Moghadam, P. (2012). Germination of Althea officinalis L. seeds under drought and salinity stress. Journal of Field Crops Research, 10, 1-10. https://doi.org/10.22067/GSC.V10I1.14368 [In Persian] Yildirim, E., & Guvenc, I. (2006). Salt tolerance of pepper cultivars during germination and seedling growth. Turkish Journal of Agriculture and Forestry, 30(5), 347-353. Zamani, S., Ghasemnezhad, A., Alizadeh, M., & Alami, M. (2018). Effect of salinity and salicylic acid on morphological and photosynthetic pigment changes of callus of artichoke (Cynara scolymus L.). Journal of Crop Breeding, 10(26), 128-138. https://doi.org/10.29252/jcb.10.26.128 [In Persian] | ||
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