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Effect of Pseudomonas flourescens, Stenotrophomonas sp. and chloro carboxylic acid on the root–knot nematode (Meloidogyne javanica) | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 11, Issue 2, January 2025, Pages 67-80 PDF (757.37 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2025.368209.383 | ||
| Authors | ||
| Mohammad Hadipoor1; hosein mirzaie najafgholi2; Kourosh Azizi* 2 | ||
| 1Msc.in Plant Pathology, Department of Plant Protection, College of Agriculture, Lorestan University, Khorramabad, Iran. | ||
| 2Assistant Professor, Department of Plant Protection, College of Agriculture, Lorestan University, Khorramabad, Iran. | ||
| Abstract | ||
| Tomato is one of the most important plants in the world. Knot–root nematodes (Meloidogyne spp.) are one of the main pathogens of tomato plants, which cause a decrease of about five percent in crops worldwide and damage of 24–38 percent on tomato plants. Considering the high cost of chemical methods and the damage they have on the environment and human health, it is necessary to have an alternative solution, including the use of growth–promoting bacteria (PGPR) to manage these nematodes. After examining the morphology and morphometry, the nematode isolated from infected fields was identified as M. javanica. In the current study, after isolation of knot– root nematode from infected fields, identification and purification and propagation of nematode, the effect of two bacteria, Pseudomonas flourescens, Stenotrophomonas sp. strain LU1 in laboratory conditions, also as P. fluorescens bacteria and chloro carboxylic acid along with nematicides on tomato plants and this nematode in greenhouse conditions were investigated. After tomato greenhouse cultivation and inoculation, in the form of a completely random design in 4 replications, Data were analyzed by SAS software. Investigations carried out in the laboratory showed that, in the egg hatching test in laboratory conditions 8 days after inoculation, P. fluorescens bacteria with 50% and Stenotrophomonas sp. strain LU1 bacteria with 23% were effective in controlling the number of hatched eggs. In the test of the number of live larvae in laboratory conditions 3 days after inoculation, P. fluorescens bacteria was able to kill 31% and Stenotrophomonas sp. strain LU1 with 0.8% mortality in larvae. In greenhouse conditions, in the test of growth indicators of tomato plants, including plant height and root length, plant and root fresh weight, plant and root dry weight, treatments containing P. fluorescens bacteria with and without nematodes, in the plant height index respectively 14% and 30% in the root length index 18% and 31% in the plant fresh weight index 36% and 50% in the plant dry weight index, 23% and 40% they showed a positive effect compared to control with nematodes. In the greenhouse, in the pathogenicity indicators related to the number of larvae in 100 cc of soil, the number of galls in one gram of root, the number of eggs in one gram of root, the treatment containing P. fluorescens bacteria was 76%, 33%, and 91%, respectively, and the treatment containing chloro carboxylic acid. 98%, 98%, and 99% showed control compared to the control, respectively, and in this treatment, the control rate of the nematicide used was more evaluated. The results of the present study showed the positive effect of P. fluorescens bacteria on the nematode M. javanica in laboratory and greenhouse conditions, as well as chloro carboxylic acid in greenhouse conditions, which was consistent with the conducted research. It should be noted that Stenotrophomonas sp. strain LU1 showed a positive but little effect in laboratory conditions. | ||
| Keywords | ||
| Egg hatching; greenhouse; laboratory; mortality; PGPR | ||
| References | ||
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Abo–Elyousr, K.A., Khan, Z., E–Morsi Award M. & Abedel–Moneim, M.F. 2010. Evaluation of plant extracts and Pseudomonas spp. For control of root–knot nematode, Meloidogyne incognita on tomato. Nematropica, 40(2): 289–299. Azizi, K. 2023. Effect of wood vinegar, chloro carboxylic acid and sodium dioctyl sulfosuccinate against Pratylenchoides ritteri on chickpea. Plant Protection (Scientific Journal of Agriculture), 45(4): 77–82. Badakhshan, M., Mahdikhani Moghadam, E., Baghaee Ravari, S. & Rouhani, H. 2017. The combined use of two species of Trichoderma and Bacillus subtilis in the control of tomato root–knot nematode (Meloidogyne javanica). Plant Pests and Diseases, 85(2): 269–278 (In Persian with English summary). Bagheri, N., Ahmadzadeh, M., Afsharmanesh, H., Saberbaghban, Z. & Mohammadzadeh Kashani, M. 2015. Evaluation of molecular and biological characteristics of Pseudomonas fluorescens UTPF5 biocontrol agent of Meloidogyne javanica on tomato. Cellular and Molecular Researches (Iranian Journal of Biology), 29(1): 15–32. (In Persian) Bazgir, M., Mirzaei Najafgholi, H., Azizi, K., Rouhani, N. & Alymanesh, M. 2024. The effect of plant growth–promoting bacteria on Cereal cyst nematode (Heterodera avenae) under laboratory and greenhouse conditions. Biocontrol in Plant Protection, 11(1): 83–96 Cuartero, J., & Fernández–Munoz, R. (1999) Tomato and Salinity. Scientia Horticulturae, 78: 83–125. Dashtipour, S., Sahibani, N. & Aminian, H. 2016. Application Effect of Bacillus subtilis and salicylic acid on the tomato plants against Fusarium oxysporoum f.sp. lycopersici and Meloidogyne javanica. Applied research in plant protection, 6(4): 1–10. (In Persian) Golzari, H., Panjeh kh, N., Ahmadzadeh, M., Salari, M. & Sadaghati Kharavi, I. 2011. Studying the effective role of some metabolites of Pseudomonas fluorescent bacteria in controlling the root–knot nematode Meloidogyne javanica on tomato. Iranian journal of plant protection science (Agricultural Sciences of Iran), 42(1): 113–124. (In Persian) Hosseini Nejad, A. 2004. The effect of neem derivatives, Azadirachta indica, to root–knot nematod, Meloidogyne javanica, in tomato. Journal of Plant Pests and Diseases. 72(1): 69–89. (In Persian) Hussey, RS. & Barker, KR. 1973. A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter, 57: 1025–1028. Javed, N., Gowen, S. R., Inam–ul–Haq, M. & Anwar, S. A. 2007. Protective and curative effect of neem (Azadirachta indica) formulations on the development of root–knot nematode Meloidogyne javanica in roots of tomato plants. Crop Protection, 26: 530–534. Khalighi, S., Khodakarmian, G., Tanhamaafi, Z., Hosseininejad, S. & Ghasemi, A. 2010. Inhibition of gall formation Meloidogyne javanica on tomato using fluorescent pseudomonas under greenhouse conditions. Plant Pests and Diseases, 78(2 (serial 91)), 177–198. (In Persian with English summary) Khan, A,. Shaukat, S. S., Islam, S. & Khan, A. 2012. Evaluation of Fluorescent Pseudomonad isolates for their activity against some plantparasitic nematodes. American–Eurasian. J. Agric. and Environ. Sci, 12 (11): 1496–1506. Majzoub, S., Kargrbideh, A., Taghvi, S. & Hamze Zarghani, H. 2011. Investigating the effect of paraphytic bacteria on root–knot nematode (Meloidogyne javanica) on cucumber under greenhouse conditions. Plant diseases, 48(1), 69–84. (In Persian) Merillon, J.M., & Ramawat, K.G. (eds) 2012. Plant defence: Biological control, Progress in Biological Control 12. Dordrecht, Springer Science Business Media, pp:67–107. Monazam, K., Jamali, S. & Alimi, M. 2022. Effect of Pseudomonas and Streptomyces bacteria in controlling root knot nematode (Meloidogyne incognita) in tomato under greenhouse conditions. Iran Nematodology, 1(1): 118–127. (In Persian with English summmary) Naseri Nasab, F., Sahibani, N. & Etebarian, H. 2011. Investigating the combined effect of salicylic acid and the fungus Trichoderma harzianum BI on the resistance of tomato plants against the root knot nematode Meloidogyne javanica. (Plant Protection) Iranian Plant Protection Research, 25(4), 417–425. (In Persian) Nasiri, M., Azizi, K., Hamzehzarghani, H. & Ghaderi, R. 2013. Studies on the nematicidal activity of stinging nettle (Urtica dioica) on plant parasitic nematodes, Archives Of Phytopathology and Plant Protection, 47(5): 591–599. Ramezani, B., and Mahdikhani Moghadam, E. & Rouhani, H. 2012. Evaluation of the resistance of some tomato cultivars to the root knot nematode (Meloidogyne javanica) in greenhouse conditions. Quarterly Journal of Plant Protection Studies, 27(3): 285–276. (In Persian with English summmary) Siddiqui, I., A. & Shaukat, S. 2003. "Suppression of root–knot disease by Pseudomonas fluorescens CHA0 in tomato: Importance of bacterial secondary metaboliite 2,4–diacetylphloroglucinol". Soil Biology and Biochemistry, 35: 1615–1623. Siddiqui, I. A., Hass, D. & Heeb, S. 2005. "Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against root–knot nematode, Meloidogyne incognita". Applied and Environmental Microbiology, 71: 5646–5649. Sohrabi, F., Sheikholeslami, M., Heydari, R., Rezaee, S. & Sharifi, R. 2016. Study on combined application of arbuscular mycorrhizal fungi isolates and plant growthpromoting rhizobacteria in controlling the root–knot nematode Meloidogyne javanica in tomato under greenhouse conditions. Plant diseases, 53(4): 449–462. (In Persian with English summary) Tanha, S., Bayat, F. & Jamali, H. 2018. Evaluation of tomato cultivars to root– knot nematode Meloidogjan javanica. Iranian Journal of Agricultural Plant Sciences (Agricultural Sciences of Iran), 50(1): 97–106. (In Persian with English summary) Whitehead, A.G., & Hemming, J.R. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annual Applied Biology, 55(1): 25–38. | ||
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