Optimizations of commercial culture medium of Bacillus subtilis isolate B2 and its formulation for controlling powdery mildew of cucumber at greenhouse | ||
| آفات و بیماری های گیاهی | ||
| Volume 92, Issue 2, March 2025, Pages 232-248 PDF (942.12 K) | ||
| Document Type: Pest Management | ||
| DOI: 10.22092/jaep.2025.367914.1534 | ||
| Authors | ||
| lachin mokhtarnejad* 1; Samira Shameli2; mohsen farzaneh3 | ||
| 1Plant Protection Research Department, West Azarbaijan Agricultural Research, Education and Extension Center, AREEO, Urmia, Iran. | ||
| 2Plant Protection Research Department, Golestan Agricultural Research, Education and Extension Center, AREEO, Gorgan, Iran. | ||
| 33- Associate professor, Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran. | ||
| Abstract | ||
| In this research, the possibility of mass production and formulation of bacterial isolate Bacillus subtilis B2 with antagonistic activity against powdery mildew of cucumber were investigated. In order to optimize the culture medium, screening of different carbon and nitrogen sources was performed using Design Expert® software. Based on the central composite design, optimal concentration of beet molasses and corn steep liquor (CSL) in the culture medium to produce maximum bacterial cell biomass was 4.45-6 and 3.82-6 g/liter, respectively. Based on the optimization of environment factors, the maximum bacterial biomass was obtained at a temperature of 32 °C, a stirring speed of 90 rpm, 3.5% of inoculums and pH of 6. Subsequent the biomass production, additional research was carried out with the objective of formulation the bacterial isolate. Totally 18 different formulations prepared with different carriers and adjutants. The highest population of B. subtilis B2 produced after six months storage at 4 °C and 24°C in formulation No. 3, containing sodium alginate and trihalose, with 7.9×108 and 4.2×107 CFU per gram of formulation respectively.The best formulation of B. subtilis selected based on the highest shelf life to evaluate the ability of formulations for controling powdery mildew of cucumber under greenhouse conditions. The results showed that the B. subtilis formulation and Dumark fungicide reduces the disease severity by 45.42% and 46.53%, respectively which express no significant difference between them. | ||
| Keywords | ||
| Biological control; commercialization; cucumber; powdery mildew | ||
| References | ||
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