Isolation of citrus epiphytic yeasts and identification of superior isolates in controlling green mold disease | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 11, Issue 2, January 2025, Pages 53-65 PDF (580.79 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2025.368126.382 | ||
| Authors | ||
| Sima Hatami1; Valiollah Babaiezad2; Farid Beiki Firouzjahi* 3; Zohreh Moradi4 | ||
| 1. M.Sc. Student, Department of Plant Protection, Faculty of Agricultural Sciences, University of Agricultural Sciences and Natural Resources, Sari, Mazandaran, Iran. | ||
| 2Professor, Department of Plant Protection, Faculty of Agricultural Sciences, University of Agricultural Sciences and Natural Resources, Sari, Mazandaran, Iran. | ||
| 3Assistant Professor, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Tehran, Iran. | ||
| 4Assistant Professor, Department of Plant Protection, Faculty of Agricultural Sciences, University of Agricultural Sciences and Natural Resources, Sari, Mazandaran, Iran. | ||
| Abstract | ||
| Green mold (Penicillium digitatum) is one of the most important diseases of citrus fruits during the postharvest and storage stages, which causes significant damage if not properly managed. In recent years, this pathogen has been controlled by the use of chemical fungicides. In order to reduce fungicide application and eliminate toxic residues, it is necessary to use other management methods. Use of microbial agents in biological control is one of these methods. Based on previous reports on the ability of yeasts to inhibit pathogens, the ability of native isolates to inhibit pathogen that cause green mold has been investigated. For this study, citrus fruit and leaf samples were gathered from various areas in Mazandaran province during autumn of 2023. Yeast colonies were then isolated and purified in the lab by using malt agar medium. Due to the large number of isolates, representatives of isolates were selected based on their phenotypic characteristics (shape, color and margin of the colony, physiological characteristics). The test was conducted on citrus fruits of the Thomson Novel variety and its Soluble solids concentration (SSC) content was determined to be 10 °Brix at the time of harvest. The biocontrol test was carried out on the citrus fruit with 44 yeast isolates. The fruit pieces were initially placed in a yeast suspension with a concentration of 10^8 cells per milliliter of sterile water, and after being stored for five hours at room temperature, 10 microliters of the green mold with a concentration of 10^4 cells per milliliter was added to the center of each cut piece. Fruits were stored under packinghouses conditions with over 90% humidity for six days until symptoms of disease appeared. The results of variance analysis based on the percentage of disease occurrence indicated that among the yeasts, there was a significant difference at a 1% level, regarding their ability to inhibit the green mold pathogen under storage conditions. Comparing the average percentage of disease incidence based on according to Duncan’s multiple range test at significance level 1% shows that the yeasts in the two statistical groups performed very well. Sequencing the D1/D2 domain within the Large subunit ribosomal ribonucleic acid (LSU rDNA) region was employed to detect these outstanding isolates. According to the results, the top–performing yeasts were Hanseniaspora uvarum, Hanseniaspora occidentalis, and Pichia kluyveri. So that after six days, they had complete inhibition of the occurrence and development of the pathogen compared to the control. . | ||
| Keywords | ||
| Biocontrol; Epiphytic; Orange; Penicillium digitatum; Postharvest | ||
| References | ||
|
Ahima, J., Zhang, H., Apaliya, M.T., Yang, Q. & Jiang, Z. 2020. The mechanism involved in enhancing the biological control efficacy of Rhodotorula mucilaginosa with salicylic acid to postharvest green mold decay of oranges. Journal of Food Measurement and Characterization, 14: 3146–3155. Alizadeh, M., Vasebi, Y. & Safaie, N. 2020. Microbial antagonists against plant pathogens in Iran: A review. Open Agriculture, 5: 404–440. Authority, E.F.S., Arena, M., Auteri, D., Barmaz, S., Bellisai, G., Brancato, A., Brocca, D., Bura, L., Byers, H. & Chiusolo, A. 2017. Peer review of the pesticide risk assessment of the active substance Metschnikowia fructicola NRRL Y‐27328. EFSA Journal, 15: e05084. Benbow, J.M. & Sugar, D. 1999. Fruit surface colonization and biological control of postharvest diseases of pear by preharvest yeast applications. Plant Disease, 83: 839–844. Bus, V., Bongers, A. & Risse, L. 1991. Occurrence of Penicillium digitatum and P. italicum resistant to benomyl, thiabendazole and imazalil on citrus fruit from different geographic origins. Plant Disease, 75: 1098–1100. Buzzini, P., Lachance, M.A. & Yurkov, A. 2017. Yeasts in Natural Ecosystems: Ecology. Springer. p. Cao, H., Baldini, R. L. & Rahme, L. G. 2001. Common mechanisms for pathogens of plants and animals. Annual review of phytopathology, 39: 259–284. Chen, C., Guo, J., Kahramanoǧlu, I. b., Wan, C., Gan, Z. & Chen, J. 2020. Biocontrol Bacterium Paenibacillus Brasilensis YS–1 Fermented Broth Enhances the Quality Attributes and Storability of Harvested “Newhall” Navel Oranges. ACS Food Science & Technology, 1: 88–95. Cheng, L., Nie, X., Jiang, C. & Li, S. 2019. The combined use of the antagonistic yeast Hanseniaspora uvarum with β–aminobutyric acid for the management of postharvest diseases of kiwifruit. Biological Control, 137: 104019. De Capdeville, G., Wilson, C.L., Beer, S.V. & Aist, J.R. 2002. Alternative disease control agents induce resistance to blue mold in harvested 'Red Delicious' apple fruit. Phytopathology, 92: 900–908. Droby, S., Wisniewski, M., Teixidó, N., Spadaro, D. & Jijakli, M.H. 2016. The science, development, and commercialization of postharvest biocontrol products. Postharvest Biology and Technology, 122: 22–29. Drusch, S. & Ragab, W. 2003. Mycotoxins in fruits, fruit juices, and dried fruits. Journal of food protection, 66: 1514–1527. Dukare, A.S., Paul, S., Nambi, V.E., Gupta, R.K., Singh, R., Sharma, K. & Vishwakarma, R.K. 2019. Exploitation of microbial antagonists for the control of postharvest diseases of fruits: A review. Critical reviews in food science and nutrition, 59: 1498–1513. Felsenstein, J. 1989. Mathematics vs. evolution: mathematical evolutionary theory. Science, 246: 941–942. Ghasemi, S., Etebarian, H., Sahebani, N. & Aminian, H. 2011, Control of blue mold on orange fruit by yeast, Metschnikowia pulcherrima (m54) and induction of b 1,3–glucanase enzyme in albedo and flavedo tissues. 27–28 July, Biological Control Development, Tehran, Iran., 477. Hammami, R., Oueslati, M., Smiri, M., Nefzi, S., Ruissi, M., Comitini, F., Romanazzi, G., Cacciola, S. O. & Sadfi Zouaoui, N. 2022. Epiphytic Yeasts and Bacteria as Candidate Biocontrol Agents of Green and Blue Molds of Citrus Fruits. Journal of fungi, 8: 818. Hernandez–Montiel, L.G., Droby, S., Preciado–Rangel, P., Rivas–García, T., González–Estrada, R. R., Gutiérrez–Martínez, P. & Ávila–Quezada, G.D. 2021. A sustainable alternative for postharvest disease management and phytopathogens biocontrol in fruit: Antagonistic yeasts. Plants, 10: 2641. Holmes, G.J. & Eckert, J.W. 1999. Sensitivity of Penicillium digitatum and P. italicum to postharvest citrus fungicides in California. Phytopathology, 89: 716–721. Ismail, M. & Zhang, J. 2004. Post–harvest citrus diseases and their control. Outlooks on Pest Management, 15: 29–35. Jukes, T.H. & Cantor, C.R. 1969. Evolution of protein molecules. Mammalian protein metabolism, 3: 21–132. Kassim, A., Workneh, T.S. & Laing, M. D. 2020. A review of the postharvest characteristics and pre–packaging treatments of citrus fruit. AIMS Agriculture and Food, 5: 337–364. Li, W., Zhang, H., Li, P., Apaliya, M.T., Yang, Q., Peng, Y. & Zhang, X. 2016. Biocontrol of postharvest green mold of oranges by Hanseniaspora uvarum Y3 in combination with phosphatidylcholine. Biological Control, 103: 30–38. Li, X., Wang, D., Cai, D., Zhan, Y., Wang, Q. & Chen, S. 2017. Identification and high–level production of pulcherrimin in Bacillus licheniformis DW2. Appl Biochem Biotechnol, 183: 1323–1335. Liu, J., Sui, Y., Wisniewski, M., Droby, S. & Liu, Y. 2013. Utilization of antagonistic yeasts to manage postharvest fungal diseases of fruit. International Journal of Food Microbiology, 167: 153–160. Liu, Y., Wang, W., Zhou, Y., Yao, S., Deng, L. & Zeng, K. 2017. Isolation, identification and in vitro screening of Chongqing orangery yeasts for the biocontrol of Penicillium digitatum on citrus fruit. Biological Control, 110: 18–24. Mewa Ngongang, M., Du Plessis, H.W., Boredi, C.S., Hutchinson, U.F., Ntwampe, K.S., Okudoh, V.I. & Jolly, N.P. 2021. Physiological and antagonistic properties of Pichia kluyveri for curative and preventive treatments against post–harvest fruit fungi. Polish Journal of Food and Nutrition Sciences, 71: 245–253. Noreen, R., Ali, S. A., Hasan, K.A., Sultana, V., Ara, J. & Ehteshamul–Haque, S. 2019. Evaluation of biocontrol potential of epiphytic yeast against postharvest Penicillium digitatum rot of stored Kinnow fruit (Citrus reticulata) and their effect on its physiochemical properties. Postharvest Biology and Technology, 148: 38–48. Oztekin, S., Dikmetas, D.N., Devecioglu, D., Acar, E.G. & Karbancioglu–Guler, F. 2023. Recent insights into the use of antagonistic yeasts for sustainable biomanagement of postharvest pathogenic and mycotoxigenic fungi in fruits with their prevention strategies against mycotoxins. J Agric Food Chem, 71: 9923–9950. Prendes, L.P., Merín, M.G., Zachetti, V.G. L., Pereyra, A., Ramirez, M.L. & Morata de Ambrosini, V.I. 2021. Impact of antagonistic yeasts from wine grapes on growth and mycotoxin production by Alternaria alternata. Journal of applied microbiology, 131: 833–843. Pusey, P. L. & Wilson, C. 1984. Postharvest biological control of stone fruit brown rot by Bacillus subtilis. Plant Disease, 68: 753–756. Qin, X., Xiao, H., Xue, C., Yu, Z., Yang, R., Cai, Z. & Si, L. 2015. Biocontrol of gray mold in grapes with the yeast Hanseniaspora uvarum alone and in combination with salicylic acid or sodium bicarbonate. Postharvest Biology and Technology, 100: 160–167. Sanzani, S. M., Reverberi, M. & Geisen, R. 2016. Mycotoxins in harvested fruits and vegetables: Insights in producing fungi, biological role, conducive conditions, and tools to manage postharvest contamination. Postharvest Biology and Technology, 122: 95–105. Sipiczki, M. 2020. Metschnikowia pulcherrima and related pulcherrimin–producing yeasts: Fuzzy species boundaries and complex antimicrobial antagonism. Microorganisms, 8: 1029. Smilanick, J., Mackey, B., Reese, R., Usall, J. & Margosan, D. 1997. Influence of concentration of soda ash, temperature, and immersion period on the control of postharvest green mold of oranges. Plant Disease, 81: 379–382. Solairaj, D., Legrand, N. N. G., Yang, Q. & Zhang, H. 2020. Isolation of pathogenic fungi causing postharvest decay in table grapes and in vivo biocontrol activity of selected yeasts against them. Physiological and Molecular Plant Pathology, 110: 101478. Spencer, D. M., Spencer, J. F. T., De Figueroa, L. & Heluane, H. 1992. Yeasts associated with rotting citrus fruits in Tucumán, Argentina. Mycol Res, 96: 891–892. Statistics of the ministry of agriculture–Jahad. 2024. Report on garden, mushroom and greenhouse products. Deputy for statistics, center for statistics, information technology and communications. 400 p. Taqarort, N., Echairi, A., Chaussod, R., Nouaim, R., Boubaker, H., Ait Ben Aoumar, A. & Boudyach, H. 2008. Screening and identification of epiphytic yeasts with potential for biological control of green mold of citrus fruits. World Journal of Microbiology and Biotechnology, 24. Themen, D. 2014. Reduction of food losses and waste in Europe and Central Asia for improved food security and agrifood chain efficiency. 85. https://openknowledge.fao.org/handle/20.500.14283/au844e Vilaplana, R., Cifuentes, C., Vaca, L., Cevallos–Cevallos, J. M. & Valencia–Chamorro, S. 2020. Curative activity of possible biocontrol agents in the postharvest of yellow pitahaya and organic banana. Postharvest Biology and Technology, 159: 111030. Wang, Y., Yu, T., Li, Y., Cai, D., Liu, X., Lu, H. & Zheng, X. 2009. Postharvest biocontrol of Alternaria alternata in Chinese winter jujube by Rhodosporidium paludigenum. Journal of applied microbiology, 107: 1492–1498. Windholtz, S., Redon, P., Lacampagne, S., Farris, L., Lytra, G., Cameleyre, M., Barbe, J.–C., Coulon, J., Thibon, C. & Masneuf–Pomarede, I. 2021. Non–Saccharomyces yeasts as bioprotection in the composition of red wine and in the reduction of sulfur dioxide. Lwt, 149: 111781. Zhang, X., Li, B., Zhang, Z., Chen, Y. & Tian, S. 2020. Antagonistic yeasts: A promising alternative to chemical fungicides for controlling postharvest decay of fruit. Journal of fungi, 6: 158. Zhang, Y., Li, T., Liu, Y., Li, X., Zhang, C., Feng, Z., Peng, X., Li, Z., Qin, S. & Xing, K. 2019. Volatile organic compounds produced by Pseudomonas chlororaphis subsp. aureofaciens SPS–41 as biological fumigants to control Ceratocystis fimbriata in postharvest sweet potatoes. J Agric Food Chem, 67: 3702–3710. Zhimo, V. Y., Kumar, A., Biasi, A., Salim, S., Feygenberg, O., Toamy, M. A., Abdelfattaah, A., Medina, S., Freilich, S. & Wisniewski, M. 2021. Compositional shifts in the strawberry fruit microbiome in response to near–harvest application of Metschnikowia fructicola, a yeast biocontrol agent. Postharvest Biology and Technology, 175: 111469.
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