اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,049
تعداد مقالات34,082
تعداد مشاهده مقاله46,512,722
تعداد دریافت فایل اصل مقاله77,564,767
Hamedi, Azadeh, Paslari, Hamed, Azarpira, Negar, Heidari, Reza, Bordbar, Leila, Pasdaran, Ardalan. (1404). Exploring the Therapeutic Potential of Iphiona aucheri: Modulation of Nitric Oxide Levels in RAW 264.7 Macrophages and Cytotoxic Effects on MCF-7 Human Breast Cancer and HT1080 Human Fibrosarcoma Cells. سامانه مدیریت نشریات علمی, 14(4), 381-384. doi: 10.22034/jmpb.2024.367490.1798
Azadeh Hamedi; Hamed Paslari; Negar Azarpira; Reza Heidari; Leila Bordbar; Ardalan Pasdaran. "Exploring the Therapeutic Potential of Iphiona aucheri: Modulation of Nitric Oxide Levels in RAW 264.7 Macrophages and Cytotoxic Effects on MCF-7 Human Breast Cancer and HT1080 Human Fibrosarcoma Cells". سامانه مدیریت نشریات علمی, 14, 4, 1404, 381-384. doi: 10.22034/jmpb.2024.367490.1798
Hamedi, Azadeh, Paslari, Hamed, Azarpira, Negar, Heidari, Reza, Bordbar, Leila, Pasdaran, Ardalan. (1404). 'Exploring the Therapeutic Potential of Iphiona aucheri: Modulation of Nitric Oxide Levels in RAW 264.7 Macrophages and Cytotoxic Effects on MCF-7 Human Breast Cancer and HT1080 Human Fibrosarcoma Cells', سامانه مدیریت نشریات علمی, 14(4), pp. 381-384. doi: 10.22034/jmpb.2024.367490.1798
Hamedi, Azadeh, Paslari, Hamed, Azarpira, Negar, Heidari, Reza, Bordbar, Leila, Pasdaran, Ardalan. Exploring the Therapeutic Potential of Iphiona aucheri: Modulation of Nitric Oxide Levels in RAW 264.7 Macrophages and Cytotoxic Effects on MCF-7 Human Breast Cancer and HT1080 Human Fibrosarcoma Cells. سامانه مدیریت نشریات علمی, 1404; 14(4): 381-384. doi: 10.22034/jmpb.2024.367490.1798

Exploring the Therapeutic Potential of Iphiona aucheri: Modulation of Nitric Oxide Levels in RAW 264.7 Macrophages and Cytotoxic Effects on MCF-7 Human Breast Cancer and HT1080 Human Fibrosarcoma Cells

Journal of Medicinal plants and By-products
مقاله 11، دوره 14، شماره 4، مهر و آبان 2025، صفحه 381-384    اصل مقاله (904.47 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2024.367490.1798
نویسندگان
Azadeh Hamedi1، 2، 3؛ Hamed Paslari1، 4؛ Negar Azarpira5؛ Reza Heidari6؛ Leila Bordbar1، 2؛ Ardalan Pasdaran* 1، 2، 3
1Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
2Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Phytopharmaceutical Technology and Traditional Medicine Incubator, Shiraz University of Medical Sciences, Shiraz, Iran
4School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
5Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
6Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
The objective of this study was to examine phytochemical compounds and the cellular effects of the essential oil of Iphiona aucheri (Boiss.) Anderb. The essential oil of the dried aerial parts of the plant with the recovery rate of 0.1% (v/w) was extracted through hydrodistillation by a Clavenger apparatus, and the chemical compounds were identified based on gas chromatography-mass spectrometry. The anti-inflammatory and cytotoxic effects of the essential oil were examined. The Griess assay was used to test the anti-inflammatory activity on the RAW 264.7 macrophage cell line. The MTT assay was used to test the cell viability on the RAW 264.7 macrophage, the HT1080 fibrosarcoma, and the MCF-7 cell lines. The essential oil of this plant consists of sesquiterpenes and monoterpenes, with more than 60% of it made up of compounds such as limonene, α-farnesene, and trans-caryophyllene. The essential oil demonstrated its anti-inflammatory effect on macrophage cells (RAW 264.7) by reducing nitric oxide production at concentrations below 100 µg/mL.  Increasing the cell viability percentage of macrophage cells along with reducing the NO release rate at a concentration of 6.25 μg/mL suggested promising anti-inflammatory effects. The essential oil was cytotoxic to the human fibrosarcoma (HT1080) with an IC50 of 47.94±1 µg/mL.
کلیدواژه‌ها
Iphiona aucheri؛ Anti-inflammatory؛ Nitric oxide؛ Cell viability
مراجع
  1. Hamedi A., Bayat M., Asemani Y., Amirghofran Z. A review of potential anti-cancer properties of some selected medicinal plants grown in Iran. J. Herb. Med. 2022; 33:100557.
  2. Khan R.A., Shah M.A.R., Ahmed M. Phytochemical assessment of Iphiona aucheri (Bioss.) Anderb. and its cytotoxic, antioxidant and antidiabetic activities. Bangladesh J. Bot. 2020;44(4):913-18.
  3. Safa O., Soltanipoor M.A., Rastegar S., Kazemi M., Dehkordi K.N., Ghannadi A. An ethnobotanical survey on hormozgan province, Iran. AJP. 2013;3(1):64.
  4. Sadeghi Z., Kuhestani K., Abdollahi V., Mahmood A. Ethnopharmacological studies of indigenous medicinal plants of Saravan region, Baluchistan, Iran. J. Ethnopharmacol. 2014;153(1):111-18.
  5. Roeder E., Bourauel T., Meier U., Wiedenfeld H. Diterpene glycosides from Iphiona aucheri. Phytochem. 1994;37(2):353-55.
  6. Wernery U., Bourauel T., Roeder E., Meier U., Wiedenfeld H. Toxic dipertene glycosides from Iphiona aucheri. Fitoterapia. 1997; 68:278-80.
  7. Kakar S.A., Tareen R.B., Kakar M.A., Jabeen H., Kakar S., Al-Kahraman Y., Shafee M. Screening of antibacterial activity of four medicinal plants of Balochistan-Pakistan. Pak. J. Bot. 2012;44(2010):245-50.
  8. Zai J.A., Khan M.R., Mughal Z.u.N., Batool R., Naz I., Maryam S., Zahra Z. Methanol extract of Iphiona aucheri ameliorates CCl4 induced hepatic injuries by regulation of genes in rats. Toxicol. Res. 2019;8(6):815-32.
  9. Shah M.A.R., Khan R.A., Ahmed M. Anti-diabetic activity of Iphiona aucheri leaf extract. Bangladesh J. Pharmacol. 2020;15(4):99-109.
  10. Rustaiyan A., Azar P.A., Moradalizadeh M., Masoudi S., Ameri N. Volatile constituents of three compositae herbs: Anthemis altissima L. var. altissima Conyza Canadensis (L.) Cronq. and Grantina aucheri Boiss. Growing Wild in Iran. J. Essent. Oil Res. 2004;16(6):579-81.
  11. Mojab F., Hamedi A., Nickavar B., Javidnia K. Hydrodistilled volatile constituents of the leaves of Daucus carota L. subsp. sativus (Hoffman.) Arcang. (Apiaceae) from Iran. J. Essent. Oil-Bear. Plants. 2008;11(3):271-77.
  12. Pasdaran A., Pasdaran A., Mamedov N. Antibacterial and antioxidant activities of the volatile composition of the flower and fruit of Solanum sisymbriifolium (Litchi Tomato). Pharm. Sci. 2017;23(1):66-71.
  13. Hamedi A., Lashgari A.P., Pasdaran A. Antimicrobial activity and analysis of the essential oils of selected endemic edible Apiaceae plants root from Caspian Hyrcanian region (North of Iran). Pharm. Sci. 2019;25(2):138-44.
  14. Lashgari A.P., Delazar A., Afshar F.H., Parsa D. Contact toxicity and chemical composition of essential oil of Acantholimon scorpius. Pharm. Sci. 2016;22(2):138-41.
  15. Taciak B., Białasek M., Braniewska A., Sas Z., Sawicka P., Kiraga Ł., Rygiel T., Król M. Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages. PLOS One. 2018;13(6):e0198943.
  16. Lee E., Lee S.-W., Adam G.O., Yoo Y.-J., Shin H.-Y., Ahn D., Jang T.-H., Oh B.-T., Park B.-Y., Kim I.-S. Anti-Inflammatory Effects of Aralia elata Extract Against Dextran Sodium Sulfate-Induced Colitis in Mice and Raw 264.7 Macrophage Cells Exposed to Lipopolysaccharide: First Report. Nat. Prod. Commun. 2022;17(9):1934578X221126047.
  17. Billack B. Macrophage activation: role of toll-like receptors, nitric oxide, and nuclear factor kappa B. Am. J. Pharm. Educ. 2006;70(5).
  18. Sharifi-Rad M., Mohanta Y.K., Pohl P., Jaradat N., Aboul-Soud M.A., Zengin G. Variation of phytochemical constituents, antioxidant, antibacterial, antifungal, and anti-inflammatory properties of Grantia aucheri (Boiss.) at different growth stages. Microb. Pathog. 2022; 172:105805.
  19. Chan W.-K., Tan L.T.-H., Chan K.-G., Lee L.-H., Goh B.-H. Nerolidol: a sesquiterpene alcohol with multi-faceted pharmacological and biological activities. Molecules. 2016;21(5):529.
  20. Gertsch J., Leonti M., Raduner S., Racz I., Chen J.-Z., Xie X.-Q., Altmann K.-H., Karsak M., Zimmer A. Beta-caryophyllene is a dietary cannabinoid. PNAS. 2008;105(26):9099-104.
  21. Yu L., Yan J., Sun Z. D-limonene exhibits anti-inflammatory and antioxidant properties in an ulcerative colitis rat model via regulation of iNOS, COX-2, PGE2 and ERK signaling pathways. Mol. Med. Rep. 2017;15(4):2339-46.
  22. Zhang N., Bian Y., Yao L. Essential oils of Gardenia jasminoides J. Ellis and Gardenia jasminoides f. longicarpa ZW Xie & M. Okada flowers: Chemical characterization and assessment of anti-inflammatory effects in alveolar macrophage. Pharmaceutics. 2022;14(5):966.
آمار
تعداد مشاهده مقاله: 209
تعداد دریافت فایل اصل مقاله: 230


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب