The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida | ||
| علوم و فناوری بذر ایران | ||
| Article 1, Volume 14, Issue 1 - Serial Number 37, March 2025, Pages 1-16 PDF (759.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.22092/ijsst.2024.364475.1510 | ||
| Authors | ||
| Leyla Cheheltanan1; Ali Tehranifar* 2; Mahmoud Shoor3; Seyyed Hossein Neamati4; Saeed Khosravi5 | ||
| 1Ph.D., Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 2Professor, Department of Horticultural Science and landscape, Faculty of Agriculture, Ferdowsi University of Mashhad,, Mashhad, Iran. | ||
| 3Associate Professor, Department of Horticultural Science and Landscape, Faculty of Agriculture,, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 4Assistant Professor, Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 5Ph.D. Student, Department of Horticultural Science and Landscape, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| Abstract | ||
| Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed. | ||
| Keywords | ||
| α-amylase; β-amylase; germination percentage; soluble sugars content | ||
| References | ||
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Achrem, M., Stępień, E., & Kalinka, A. (2023). Epigenetic changes occurring in plant inbreeding. International Journal of Molecular Sciences, 24(6), 5407. https://doi.org/10.3390/ijms24065407 AOAC (Association of Official Analytical Chemists). (1995). Official methods of analysis (16th ed.). AOAC International. Aziz, T., & Pekşen, E. (2020). Seed priming with gibberellic acid rescues chickpea (Cicer arietinum L.) from chilling stress. Acta Physiologiae Plantarum, 42, 1–10. https://doi.org/10.1007/s11738-020-03134-y Bakht, J., Shafi, M., Shah, R., & Munir, I. (2011). Response of maize cultivars to various priming sources. Pakistan Journal of Botany, 43(1), 205–212. Balaguera-López, H. E., Cárdenas-Hernández, J. F., & Álvarez-Herrera, J. G. (2009). Effect of gibberellic acid (GA3) on seed germination and growth of tomato (Solanum lycopersicum L.). In Acta Horticulturae, 821, 141–148. https://doi.org/10.17660/ActaHortic.2009.821.15 Bhargava, B., Gupta, Y. C., Dhiman, S. R., & Sharma, P. (2015). Effect of seed priming on germination, growth, and flowering of snapdragon (Antirrhinum majus L.). National Academy Science Letters, 38, 81–85. https://doi.org/10.1007/s40009-014-0298-4 Cornea-Cipcigan, M., Pamfil, D., Sisea, C. R., & Mărgăoan, R. (2020). Gibberellic acid can improve seed germination and ornamental quality of selected Cyclamen species grown under short and long days. Agronomy, 10(4), 516. https://doi.org/10.3390/agronomy10040516 Farhoudi, R., & Lee, D. J. (2014). Halopriming corn seeds improves seed emergence and carbohydrate metabolism under salinity stress. Seed Science and Technology, 42(3), 461–465. https://doi.org/10.15258/sst.2014.42.3.13 Farooq, M., Barsa, S. M., & Wahid, A. (2006). Priming of field-sown rice seed enhances germination, seedling establishment, allometry, and yield. Plant Growth Regulation, 49, 285–294. https://doi.org/10.1007/s10725-006-9138-y Farooq, M., Basra, S. M., Wahid, A., & Ahmad, N. (2010). Changes in nutrient homeostasis and reserves metabolism during rice seed priming: Consequences for seedling emergence and growth. Agricultural Sciences in China, 9(2), 191–198. https://doi.org/10.1016/S1671-2927(09)60083-3 Ganga, M., Jayalakshmi, S., Jegadeeswari, V., Padmadevi, K., & Jawaharlal, M. (2011). Petunia. In C. Kole (Ed.), Wild crop relatives: Genomic and breeding resources—Plantation and ornamental crops (pp. 209–242). Springer. https://doi.org/10.1007/978-3-642-21201-7_11 Ge, N., Jia, J. S., Yang, L., Huang, R. M., Wang, Q. Y., Chen, C., Meng, Z. G., Li, L. G., & Chen, J. W. (2023). Exogenous gibberellic acid shortens the after-ripening process and promotes seed germination in the medicinal plant Panax notoginseng. BMC Plant Biology, 23(1), 67. https://doi.org/10.1186/s12870-023-04084-3 Ghasemi-Ghahsareh, M., & Kafi, M. (2008). Scientific and practical floriculture (2nd ed.). Publications Golbon. [In Persian] Ghassemi-Golezani, K., Chadordooz-Jeddi, A., Nasrollahzadeh, S., & Moghaddam, M. (2010). Effects of hydro-priming duration on seedling vigor and grain yield of pinto bean (Phaseolus vulgaris L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 109–113. https://doi.org/10.15835/nbha3813475 Godoy, A. R., Oviedo, V. R. S., Castro, M. M., & Cardoso, A. I. I. (2006). Effect of inbreeding on seed yield of "caipira" cucumber. Bragantia, 65, 569–573. https://doi.org/10.1590/S0006-87052006000400006 Guo, Y., & Lin, H. (2009). Regulatory effect of GA and KT on seeds germination of Nitraria tangutorum Bobr. Zhongguo Shengtai Nongye Xuebao/Chinese Journal of Eco-Agriculture, 17(6), 1196–1199. https://doi.org/10.3724/SP.J.1011.2009.01196 He, Y., Cheng, J., He, Y., Yang, B., Cheng, Y., Yang, C., Zhang, H., & Wang, Z. (2019). Influence of isopropylmalate synthase OsIPMS1 on seed vigor associated with amino acid and energy metabolism in rice. Plant Biotechnology Journal, 17(2), 322–337. https://doi.org/10.1111/pbi.12979 Hossain, M., Arefin, M., Khan, B., & Rahman, M. (2005). Effects of seed treatments on germination and seedling growth attributes of horitaki (Terminalia chebula Retz.) in the nursery. Research Journal of Agricultural and Biological Sciences, 1(2), 135–141. Jaques, L., Carvalho, I. R., Szareski, V. J., Pimentel, J. R., Troyjack, C., Dellagostin, S. M., Mendonça, M. T., Rosa, T. C. d., Villela, F. A., & Souza, V. Q. d. (2019). Gibberellic acid utilization in seeds and plants of beans: Effect on growth and seeds’ physiological quality. Journal of Agricultural Science, 11(2), 541. https://doi.org/10.5539/jas.v11n2p541 Kaamali, M., Shour, M., Neamati, S. H., Lakzian, A., & Khazaei, H. (2019). Effect of drought stress on physio-morphological characteristics and proline content of three petunia varieties. Journal of Horticultural Science, 32(4), 519–529. https://doi.org/10.22067/jhorts4.v32i2.58195 [In Persian] Kaczmarska, E., Gawroński, J., Dyduch-Siemińska, M., Szypiło, P., & Szafrańska, B. (2014). Inbreeding depression for seed germination and seedling vigor in strawberry (Fragaria × ananassa Duch.). Folia Horticulturae, 26, 133–138. https://doi.org/10.1515/fhort-2015-0004 Kader, M. A. (2005). A comparison of seed germination calculation formulae and the associated interpretation of resulting data. Proceedings of the Royal Society of London, 138, 65–75. https://doi.org/10.5962/p.361564 Kalsa, K. K., & Abebie, B. (2012). Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). African Journal of Agricultural Research, 7(21), 3202–3208. https://doi.org/10.5897/AJAR11.1489 Kurup, S. S., Al Amouri, A. W. H., & Jaleel, C. A. (2013). Effect of seed programming on photosynthetic pigments, biochemical constituents, and mineral nutrients in petunia (Petunia hybrida Hort. Vilm-Andr.). Journal of Food, Agriculture & Environment, 11(1), 1076–1079. Li, Z., Lu, G. Y., Zhang, X. K., Zou, C. S., Cheng, Y., & Zheng, P. Y. (2010). Improving drought tolerance of germinating seeds by exogenous application of gibberellic acid (GA3) in rapeseed (Brassica napus L.). Seed Science and Technology, 38(2), 432–440. https://doi.org/10.15258/sst.2010.38.2.16 Mebratu, A. (2022). Potassium nitrate priming effect on the germination of tomato (Lycopersicum esculentum Mill) cvs. “Mersa” and “Tekeze-1.” International Journal of Agronomy, 22, 61–66. https://doi.org/10.1155/2022/4970107 Mitchum, M. G., Yamaguchi, S., Hanada, A., Kuwahara, A., Yoshioka, Y., Kato, T., Tabata, S., Kamiya, Y., & Sun, T. P. (2006). Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development. The Plant Journal, 45(5), 804–818. https://doi.org/10.1111/j.1365-313X.2005.02642.x Moaaz Ali, M., Javed, T., Mauro, R. P., Shabbir, R., Afzal, I., & Yousef, A. F. (2020). Effect of seed priming with potassium nitrate on the performance of tomato. Agriculture, 10(11), 487–498. https://doi.org/10.3390/agriculture10110498 Nejad, S. R., Bistgani, Z. E., & Barker, A. V. (2022). Enhancement of seed germination of yarrow with gibberellic acid, potassium nitrate, scarification, or hydropriming. Journal of Crop Improvement, 36(3), 335–349. https://doi.org/10.1080/15427528.2021.1968553 Noor-un-Nisa, M., Gandahi, M., Pahoja, V. M., & Nasim, S. (2013). Response of seed priming with boron on germination and seedling sprouts of broccoli. International Journal of Agricultural Science and Research, 3(2), 183–194. Pounders, C., Reed, S., & Pooler, M. (2006). Comparison of self- and cross-pollination on pollen tube growth, seed development, and germination in crapemyrtle. HortScience, 41(3), 575–578. https://doi.org/10.21273/HORTSCI.41.3.575 Saeidi, G., Etemadi, N., Razmjoo, J., & Khajepour, M. R. (2007a). Inbreeding effects on various quantitative traits of Petunia hybrida L. Journal of Genetics and Breeding, 61(1), 19–26. Saeidi, G., Etemadi, N., Razmjoo, J., & Khajepour, M. R. (2007b). Investigation of seed production and stability of desirable traits in some ornamental plants and weed control methods. Parks and Green Space Organization of Isfahan Municipality.[In Persian] Šćepanović, M., Košćak, L., Pismarović, L., & Šoštarčić, V. (2022). Stimulation of germination of freshly collected and cold-stored seeds of Ambrosia artemisiifolia L. Plants, 20(11), 1888. https://doi.org/10.3390/plants11141888 Jamali, S., Ahmad, W., Ul-Allah, S., Wahocho, N. A., Jamali, M. F., & Shah, S. A. (2023). Comparative effect of varieties and types of containers on seed germination and seedling growth of geranium (Pelargonium graveolens). Seeds, 2(1), 165–176. https://doi.org/10.3390/seeds2010013 Sisodia, A., Padhi, M., Pal, A., Barman, K., & Singh, A. K. (2018). Seed priming on germination, growth, and flowering in flowers and ornamental trees. In Advances in Seed Priming (pp. 263–288). https://doi.org/10.1007/978-981-13-0032-5_14 Song, Q., Cheng, S., Chen, Z., Nie, G., Xu, F., Zhang, J., Zhou, M., Zhang, W., Liao, Y., & Ye, J. (2019). Comparative transcriptome analysis revealing the potential mechanism of seed germination stimulated by exogenous gibberellin in Fraxinus hupehensis. BMC Plant Biology, 19, 1–17. https://doi.org/10.1186/s12870-019-1801-3 Sukifto, R., Nulit, R., Kong, Y. C., Sidek, N., Mahadi, S. N., Mustafa, N., & Razak, R. A. (2020). Enhancing germination and early seedling growth of Malaysian indica rice (Oryza sativa L.) using hormonal priming with gibberellic acid (GA3). AIMS Agriculture & Food, 5(4), 649–668. Wahocho, N. A., Laghari, R. M., Talpur, K. H., Jamali, M. F., Ahmad, W., Shah, A. N., Otho, S. A., Shar, P. A., & Wahocho, S. A. (2023). Seed germination and vegetative growth of Petunia hybrida genotypes to salt stress. Journal of Applied Research in Plant Science, 4(02), 553–565. https://doi.org/10.38211/joarps.2023.04.02.173 Wang, Y., Yuetao, W., Ruifang, Y., Fuhua, W., Jing, F., Wenbo, Y., Tao, B., Shengxuan, W., & Haiqing, Y. (2021). Effects of gibberellin priming on seedling emergence and transcripts involved in mesocotyl elongation in rice under deep direct-seeding conditions. Journal of Zhejiang University-SCIENCE A, 22(12), 1002. https://doi.org/10.1631/jzus.B2100174 Worthington, V. (1993). Worthington enzyme manual: Enzymes and related biochemicals. Worthington Biochemical Corporation. | ||
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