| Methicillin-resistant strains of Staphylococcus aureus strains pose a critical challenge to healthcare, necessitating the development of novel therapeutic approaches. This study investigated the potential of secondary metabolites from Mentha cordifolia Opiz and Mentha arvensis L. to modulate antibiotic resistance in methicillin-resistant S. aureus clinical isolates. A rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay was employed to evaluate the antibacterial activity, antibiotic resistance-modulating activities, and effects of Mentha cordifolia Opiz and Mentha arvensis L. extracts. This study focused on the interaction between these extracts and oxacillin, an antibiotic that typically exhibits high minimum inhibitory concentrations (MICs) against methicillin-resistant Staphylococcus aureus. Remarkably, the addition of the buffered crude extracts of Mentha cordifolia and Mentha arvensis to oxacillin resulted in significant modulatory activity with a modulatory factor of 2 to 413.3 at a concentration of 125 µg/mL. This modulation was observed as a decrease in oxacillin MIC against the tested methicillin-resistant S. aureus strains. Moreover, both Mentha cordifolia and Mentha arvensis demonstrated potent modulatory effects, accounting for 71% of all tested methicillin-resistant phenotypes. The results of this study open new avenues for combating the growing threat of antibiotic-resistant bacterial infections in the healthcare setting. By potentially enhancing the efficacy of existing antibiotics, such as oxacillin, this approach could lead to improved treatment outcomes for patients with methicillin-resistant S. aureus infections. |
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