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Mokari, Saba, Azizi Jalilian, Farid, Shokri, Somayeh, teimoori, Ali, Mahmoudvand, Shahab. (1404). Isolation and Characterization of Lytic Bacteriophages against Antibiotic-Resistant Klebsiella Pneumoniae from Wastewater Samples. سامانه مدیریت نشریات علمی, 80(4), 1005-1014. doi: 10.32592/ARI.2025.80.4.1005
Saba Mokari; Farid Azizi Jalilian; Somayeh Shokri; Ali teimoori; Shahab Mahmoudvand. "Isolation and Characterization of Lytic Bacteriophages against Antibiotic-Resistant Klebsiella Pneumoniae from Wastewater Samples". سامانه مدیریت نشریات علمی, 80, 4, 1404, 1005-1014. doi: 10.32592/ARI.2025.80.4.1005
Mokari, Saba, Azizi Jalilian, Farid, Shokri, Somayeh, teimoori, Ali, Mahmoudvand, Shahab. (1404). 'Isolation and Characterization of Lytic Bacteriophages against Antibiotic-Resistant Klebsiella Pneumoniae from Wastewater Samples', سامانه مدیریت نشریات علمی, 80(4), pp. 1005-1014. doi: 10.32592/ARI.2025.80.4.1005
Mokari, Saba, Azizi Jalilian, Farid, Shokri, Somayeh, teimoori, Ali, Mahmoudvand, Shahab. Isolation and Characterization of Lytic Bacteriophages against Antibiotic-Resistant Klebsiella Pneumoniae from Wastewater Samples. سامانه مدیریت نشریات علمی, 1404; 80(4): 1005-1014. doi: 10.32592/ARI.2025.80.4.1005

Isolation and Characterization of Lytic Bacteriophages against Antibiotic-Resistant Klebsiella Pneumoniae from Wastewater Samples

Archives of Razi Institute
مقاله 22، دوره 80، شماره 4، مهر و آبان 2025، صفحه 1005-1014    اصل مقاله (514.27 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.4.1005
نویسندگان
Saba Mokari1؛ Farid Azizi Jalilian* 1؛ Somayeh Shokri2؛ Ali teimoori3؛ Shahab Mahmoudvand* 2
1Department of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2Department of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Infectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
3Department of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
چکیده
Introduction: One of the most important global health problems in the last decade is the increasing prevalence of antibiotic-resistant Klebsiella pneumoniae. Phage therapy is considered a highly efficient alternative to treat antibiotic-resistant bacteria. Therefore, this study aimed isolate lytic phages against clinical strains of antibiotic-resistant Klebsiella pneumoniae from wastewater samples.
Materials and methods: In this study, different samples were collected and the presence of phage was confirmed by a double layer test. The protein and genomic structure were verified using SDS PAGE and RAPD PCR techniques. Transmission electron microscopy (TEM) was used for the evaluation of morphology. The isolated phage's stability was assessed by subjecting it to different temperature and pH conditions. An adsorption assay test was conducted to the duration of phage absorption to corresponding host bacteria.
Result: In this study, we isolated three lytic phages (k.8, k.22, and k.34) that target K .pneumoniae. Transmission electron microscopy (TEM) results indicated that all three phages belong to the Siphoviridae family. Additionally, our investigation demonstrated that these phages remain stable within a temperature range of 4°C to 50°C and a pH range of 5.0 to 9.0. The adsorption assay tests showed that the isolated phages were able to attach to their corresponding host bacteria within 6 to 9 minutes.
Conclusion: The present study demonstrated that the isolated phages targeting K. pneumoniae exhibited a favorable survival rate when subjected to pH and thermal treatments. Furthermore, the isolated phages exhibited a short latent period, indicating rapid adsorption to the host surface. These findings suggest the potential application of these phages in phage therapy. However, further studies in the field of phage therapy are required.
کلیدواژه‌ها
Klebsiella pneumoniae؛ Lytic phage؛ Phage therapy؛ Wastewater
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مراجع
  1. Gholizadeh O, Ghaleh HEG, Tat M, Ranjbar R, Dorostkar R. The potential use of bacteriophages as antibacterial agents against Klebsiella pneumoniae. Virol J. 2024;21(1):191.https://doi.org/10.1186/s12985-024-02450-7.
  2. Abbas R, Chakkour M, Zein El Dine H, Obaseki EF, Obeid ST, Jezzini A, et al. General Overview of Klebsiella pneumonia: Epidemiology and the Role of Siderophores in Its Pathogenicity. Biology. 2024;13(2):78
  3. Kot B, Witeska M. Review: Antimicrobial resistance of Klebsiella pneumoniae isolated from poultry, cattle and pigs. animal. 2024;18(11):101345.https://doi.org/https://doi.org/10.1016/j.animal.2024.101345.
  4. Li Y, Kumar S, Zhang L. Mechanisms of Antibiotic Resistance and Developments in Therapeutic Strategies to Combat Klebsiella pneumoniae Infection. Infect Drug Resist. 2024;17:1107-19.https://doi.org/10.2147/idr.S453025.
  5. Anand T, Virmani N, Kumar S, Mohanty AK, Pavulraj S, Bera BC, et al. Phage therapy for treatment of virulent Klebsiella pneumoniae infection in a mouse model. Journal of global antimicrobial resistance. 2020;21:34-41
  6. Wernicki A, Nowaczek A, Urban-Chmiel R. Bacteriophage therapy to combat bacterial infections in poultry. Virology journal. 2017;14(1):1-13
  7. Olawade DB, Fapohunda O, Egbon E, Ebiesuwa OA, Usman SO, Faronbi AO, et al. Phage therapy: A targeted approach to overcoming antibiotic resistance. Microbial Pathogenesis. 2024;197:107088.https://doi.org/https://doi.org/10.1016/j.micpath.2024.107088.
  8. Elahi Y, Nowroozi J, Fard RMN. Isolation and characterization of bacteriophages from wastewater sources on Enterococcus spp. isolated from clinical samples. Iranian Journal of Microbiology. 2021;13(5):671
  9. Townsend EM, Kelly L, Gannon L, Muscatt G, Dunstan R, Michniewski S, et al. Isolation and characterization of Klebsiella phages for phage therapy. Therapy, Applications, and Research. 2021;2(1):26-42
  10. MacNeil D, Howe M, Brill W. Isolation and characterization of lambda specialized transducing bacteriophages carrying Klebsiella pneumoniae nif genes. Journal of Bacteriology. 1980;141(3):1264-71
  11. Azizian R, Nasser A, Askari H, Kalani MT, Sadeghifard N, Pakzad I, et al. Sewage as a rich source of phage study against Pseudomonas aeruginosa PAO. Biologicals. 2015;43(4):238-41
  12. Calderon-Gonzalez R, Lee A, Lopez-Campos G, Hancock SJ, Sa-Pessoa J, Dumigan A, et al. Modelling the Gastrointestinal Carriage of Klebsiella pneumoniae Infections. mBio. 2023;14(1):e0312122.https://doi.org/10.1128/mbio.03121-22.
  13. Karampatakis T, Tsergouli K, Behzadi P. Carbapenem-Resistant Klebsiella pneumoniae: Virulence Factors, Molecular Epidemiology and Latest Updates in Treatment Options. Antibiotics (Basel). 2023;12(2).https://doi.org/10.3390/antibiotics12020234.
  14. Zalewska-Piątek B. Phage Therapy-Challenges, Opportunities and Future Prospects. Pharmaceuticals (Basel). 2023;16(12).https://doi.org/10.3390/ph16121638.
  15. Hibstu Z, Belew H, Akelew Y, Mengist HM. Phage Therapy: A Different Approach to Fight Bacterial Infections. Biologics. 2022;16:173-86.https://doi.org/10.2147/btt.S381237.
  16. Osman AH, Kotey FCN, Odoom A, Darkwah S, Yeboah RK, Dayie N, et al. The Potential of Bacteriophage-Antibiotic Combination Therapy in Treating Infections with Multidrug-Resistant Bacteria. Antibiotics (Basel). 2023;12(8):1329.https://doi.org/10.3390/antibiotics12081329.
  17. Miki K, Urita Y, Ishikawa F, Iino T, Shibahara-Sone H, Akahoshi R, et al. Effect of Bifidobacterium bifidum fermented milk on Helicobacter pylori and serum pepsinogen levels in humans. Journal of Dairy Science. 2007;90(6):2630-40
  18. Ackermann H-W. Frequency of morphological phage descriptions in 1995. Archives of virology. 1996;141:209-18
  19. Ross A, Ward S, Hyman P. More is better: selecting for broad host range bacteriophages. Frontiers in microbiology. 2016;7:217131
  20. Zhang C, Yuan J, Guo C, Ge C, Wang X, Wei D, et al. Identification and complete genome of lytic “Kp34likevirus” phage vB_KpnP_Bp5 and therapeutic potency in the treatment of lethal Klebsiella pneumoniae infections in mice. Virus research. 2021;297:198348
  21. Chan BK, Abedon ST, Loc-Carrillo C. Phage cocktails and the future of phage therapy. Future Microbiol. 2013;8(6):769-83.https://doi.org/10.2217/fmb.13.47.
  22. Wu L-T, Chang S-Y, Yen M-R, Yang T-C, Tseng Y-H. Characterization of extended-host-range pseudo-T-even bacteriophage Kpp95 isolated on Klebsiella pneumoniae. Applied and Environmental Microbiology. 2007;73(8):2532-40
  23. Jamal M, Hussain T, Rajanna Das C, Andleeb S. Isolation and Characterization of a Myoviridae MJ1 Bacteriophage Against Multi-Drug Resistant Escherichia coli 3. Jundishapur J Microbiol. 2015;8(11):e25917.https://doi.org/10.5812/jjm.25917.
  24. Qadri I, Harakeh S, Teklemariam AD, Al Amri T, Al-Hindi R. Isolation and Identification of a Wastewater Siphoviridae Bacteriophage Targeting Multidrug-resistant Klebsiella pneumoniae. Jundishapur Journal of Microbiology. 2021;14(9):e118910
  25. Kim Y, Lee S-M, Nong LK, Kim J, Kim SB, Kim D. Characterization of Klebsiella pneumoniae bacteriophages, KP1 and KP12, with deep learning-based structure prediction. Frontiers in Microbiology. 2023;13:990910
  26. Shao Y, Wang IN. Bacteriophage adsorption rate and optimal lysis time. Genetics. 2008;180(1):471-82.https://doi.org/10.1534/genetics.108.090100.
  27. Fang C, Dai X, Xiang L, Qiu Y, Yin M, Fu Y, et al. Isolation and characterization of three novel lytic phages against K54 serotype carbapenem-resistant hypervirulent Klebsiella pneumoniae. Frontiers in Cellular and Infection Microbiology. 2023;13:1265011
  28. Beaudoin RN, DeCesaro DR, Durkee DL, Barbaro SE. Isolation of a bacteriophage from sewage sludge and characterization of its bacterial host cell. River academic journal. 2007;3(1):1-8
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