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Shakib, Pegah, Dalaei Moghadam, Maryam, Hemmati, Aida, Sepahdar, Asma, Bahadorikhalili, Saeed, Rezaei, Mohammad. (1404). Antibacterial, antifungal, antibiofilm, and cytotoxicity activity of Astragalus baba-alliar extract against main causes of dental root canal infections. سامانه مدیریت نشریات علمی, 80(4), 907-916. doi: 10.32592/ARI.2025.80.4.907
Pegah Shakib; Maryam Dalaei Moghadam; Aida Hemmati; Asma Sepahdar; Saeed Bahadorikhalili; Mohammad Rezaei. "Antibacterial, antifungal, antibiofilm, and cytotoxicity activity of Astragalus baba-alliar extract against main causes of dental root canal infections". سامانه مدیریت نشریات علمی, 80, 4, 1404, 907-916. doi: 10.32592/ARI.2025.80.4.907
Shakib, Pegah, Dalaei Moghadam, Maryam, Hemmati, Aida, Sepahdar, Asma, Bahadorikhalili, Saeed, Rezaei, Mohammad. (1404). 'Antibacterial, antifungal, antibiofilm, and cytotoxicity activity of Astragalus baba-alliar extract against main causes of dental root canal infections', سامانه مدیریت نشریات علمی, 80(4), pp. 907-916. doi: 10.32592/ARI.2025.80.4.907
Shakib, Pegah, Dalaei Moghadam, Maryam, Hemmati, Aida, Sepahdar, Asma, Bahadorikhalili, Saeed, Rezaei, Mohammad. Antibacterial, antifungal, antibiofilm, and cytotoxicity activity of Astragalus baba-alliar extract against main causes of dental root canal infections. سامانه مدیریت نشریات علمی, 1404; 80(4): 907-916. doi: 10.32592/ARI.2025.80.4.907

Antibacterial, antifungal, antibiofilm, and cytotoxicity activity of Astragalus baba-alliar extract against main causes of dental root canal infections

Archives of Razi Institute
مقاله 12، دوره 80، شماره 4، مهر و آبان 2025، صفحه 907-916    اصل مقاله (491.76 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.4.907
نویسندگان
Pegah Shakib1؛ Maryam Dalaei Moghadam2؛ Aida Hemmati3؛ Asma Sepahdar4؛ Saeed Bahadorikhalili5؛ Mohammad Rezaei* 6
1Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
2Department of Endodontics, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
3Student Research Committee, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
4Department of Dental Biomaterials, Faculty of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
5Department of Electronic Engineering, Universitat Rovira i Virgili, 43007, Tarragona, Spain.
6Razi Herbal Medicines Research Center, Student Research Committee, School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran.
چکیده
The objective of endodontic treatment is paramount: to completely eradicate bacterial infection within the dental pulp and root canal system. This study aimed to evaluate the Antimicrobial, antibiofilm, and cytotoxicity activity of Astragalus baba-alliar (A. baba-alliar) extract against the main causes of dental root canal infections (Enterococcus faecalis and Candida albicans). After the preparation of the methanolic extract from A. baba-alliar, phytochemical analysis was conducted to determine the content of secondary metabolites, followed by the determination of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) against Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis). Subsequently, the ability of the methanolic extract to inhibit biofilm formation was investigated using the microtiter plate method. The cytotoxic effects of the methanolic extract on normal human gingival fibroblast cells (HGF1) and oral cancer cells (KB) were evaluated using the MTT reduction method. Based on the phytochemical results, the presence of flavonoids, terpenoids, saponins, and polysaccharides in this plant extract was confirmed. The total phenol and flavonoid content were determined to be 4.23 mg GEA/g DW and 2.61 mg QE/g DW, respectively. The methanol extract of the plant, both alone and in combination with nystatin, exhibited a significant anti-candidal effect against C. albicans, while alone and especially in combination with chlorhexidine, it demonstrated a significant antibacterial effect against E. faecalis. Moreover, the extract alone and in combination with nystatin induced biofilm formation in C. albicans with an MBIC50 of 4.6 μg/ml, 64 μg/ml, and 0.25 μg/ml, respectively. Similarly, the extract alone and combined with chlorhexidine inhibited biofilm formation in E. faecalis with a Minimum biofilm inhibitory concentration (MBIC50) of 42.6 μg/ml and 1.16 μg/ml, respectively. The calculated Selectivity Index (SI) exceeding 2 (SI=2.72) indicates the extract's selective cytotoxicity towards cancer cells while maintaining negligible toxicity towards normal. Based on the antimicrobial properties uncovered in this research, the study is anticipated to lay the groundwork for clinical trials and subsequent investigations into the plant's effective compounds. Such endeavors hold potential for application across various industrial sectors including food, pharmaceuticals, and medicine.
کلیدواژه‌ها
Astragalus baba-alliar؛ Enterococcus faecalis؛ Candida albicans
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مراجع
  1. Saha S, Samadi F, Jaiswal J, Ghoshal U. Antimicrobial activity of different endodontic sealers: An: in vitro: evaluation. Journal of Indian Society of Pedodontics and Preventive Dentistry. 2010;28(4):251-7
  2. Komabayashi T, Colmenar D, Cvach N, Bhat A, Primus C, Imai Y. Comprehensive review of current endodontic sealers. Dental materials journal. 2020;39(5):703-20
  3. Özdemir O, Kopac T. Cytotoxicity and biocompatibility of root canal sealers: A review on recent studies. Journal of Applied Biomaterials & Functional Materials. 2022;20:22808000221076325
  4. Omidi A, Mosleh H, Rahimi-Kakolaki M. Comparative Evaluation of Disinfectants’ Efficacy in Reducing Bacterial and Fungal Contamination in Livestock Feed Production. Iranian Journal of Veterinary Medicine. 2025;19(2)
  5. Wang C-H, Hsieh Y-H, Powers ZM, Kao C-Y. Defeating antibiotic-resistant bacteria: exploring alternative therapies for a post-antibiotic era. International journal of molecular sciences. 2020;21(3):1061
  6. Peidaei F, Ahari H, Anvar A, Ataei M. Nanotechnology in Food Packaging and Storage: A Review. Iranian Journal of Veterinary Medicine. 2021;15(2)
  7. Shokri H, Sharifzadeh A, Khosravi A. Antifungal activity of the Trachyspermum ammi essential oil on some of the most common fungal pathogens in animals. 2016
  8. Moradi MS, Kamkar S, Sharifzadeh A, Hassan J, Shokri H, Abbasi J. The in Vitro Effect of Berberine Sulfate and Berberine Chloride on the Growth and Aflatoxin Production by Aspergillus flavus and Aspergillus parasiticus. Iranian Journal of Veterinary Medicine. 2024;18(2):223-32
  9. Li C-X, Liu Y, Zhang Y-Z, Li J-C, Lai J. Astragalus polysaccharide: a review of its immunomodulatory effect. Archives of Pharmacal Research. 2022;45(6):367-89
  10. Du Y, Wan H, Huang P, Yang J, He Y. A critical review of Astragalus polysaccharides: from therapeutic mechanisms to pharmaceutics. Biomedicine & pharmacotherapy. 2022;147:112654
  11. Wang D, Cui Q, Yang YJ, Liu AQ, Zhang G, Yu JC. Application of dendritic cells in tumor immunotherapy and progress in the mechanism of anti-tumor effect of Astragalus polysaccharide (APS) modulating dendritic cells: a review. Biomedicine & Pharmacotherapy. 2022;155:113541
  12. Ikbal AMA, Rajkhowa A, Debnath B, Singh WS, Manna K, Bhattacharjee B, et al. Pharmacological Review on Astragalus membranaceus: Chinese Traditional Herb. Pharmacognosy Reviews. 2022;16(32)
  13. Asgharian P, Ghalbi Z, Sarvari Y, Delazar A, Bamdad S, Asnaashari S. Phytochemical and antimalarial effects of Ecballium elaterium (L.) rich. growing in Iran. Jundishapur J Nat Pharm Prod. 2021;16(2):e103156
  14. Phuyal N, Jha PK, Raturi PP, Rajbhandary S. Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC. The Scientific World Journal. 2020;2020(1):8780704
  15. Falahati M, Sepahvand A, Mahmoudvand H, Baharvand P, Jabbarnia S, Ghojoghi A, et al. Evaluation of the antifungal activities of various extracts from Pistacia atlantica Desf. Current medical mycology. 2015;1(3):25
  16. Espinel-Ingroff A, Cantón E. Antifungal susceptibility testing of yeasts. Antimicrobial susceptibility testing protocols Taylor & Francis Group LLC. 2007:173-208
  17. Tsai T-H, Tsai T-H, Chien Y-C, Lee C-W, Tsai P-J. In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs. Food Chemistry. 2008;110(4):859-64
  18. Nabavizadeh M, Abbaszadegan A, Gholami A, Sheikhiani R, Shokouhi M, Shams MS, et al. Chemical constituent and antimicrobial effect of essential oil from Myrtus communis leaves on microorganisms involved in persistent endodontic infection compared to two common endodontic irrigants: an: in vitro: study. Journal of Conservative Dentistry and Endodontics. 2014;17(5):449-53
  19. Dohare S, Dubey SD, Kalia M, Verma P, Pandey H, Singh NK, et al. Anti-biofilm activity of Eucalyptus globulus oil encapsulated silica nanoparticles against E. coli biofilm. International Journal of Pharmaceutical Sciences and Research. 2014;5(11):5011
  20. Alizadegan F, Aghaei M, Kumar SJ, Saadatmand M, Kumar SA. In vitro and in vivo antileishmanial effects of Nectaroscordum koelzi extract against Leishmania major. Journal of Parasitic Diseases. 2023;47(3):683-8
  21. Gunjan M, Naing TW, Saini RS, Ahmad A, Naidu JR, Kumar I. Marketing trends & future prospects of herbal medicine in the treatment of various disease. World Journal of Pharmaceutical Research. 2015;4(9):132-55
  22. Nayeem N, Imran M, Asdaq SMB, Rabbani SI, Alanazi FA, Alamri AS, et al. Total phenolic, flavonoid contents, and biological activities of stem extracts of Astragalus spinosus (Forssk.) Muschl. grown in Northern Border Province, Saudi Arabia. Saudi Journal of Biological Sciences. 2022;29(3):1277-82
  23. Asgarpanah J, Motamed SM, Farzaneh A, Ghanizadeh B, Tomraee S. Antioxidant activity and total phenolic and flavonoid content of Astragalus squarrosus Bunge. African Journal of Biotechnology. 2011;10(82):19176-80
  24. Kumar S, Abedin MM, Singh AK, Das S. Role of phenolic compounds in plant-defensive mechanisms. Plant phenolics in sustainable agriculture: volume 1: Springer; 2020. p. 517-32.
  25. Rempe CS, Burris KP, Lenaghan SC, Stewart Jr CN. The potential of systems biology to discover antibacterial mechanisms of plant phenolics. Frontiers in microbiology. 2017;8:422
  26. Ecevit K, Barros AA, Silva JM, Reis RL. Preventing microbial infections with natural phenolic compounds. Future Pharmacology. 2022;2(4):460-98
  27. Jaradat NA, Zaid AN, Abuzant A, Khalaf S, Abu-Hassan N. Phytochemical and biological properties of four Astragalus species commonly used in traditional Palestinian medicine. European Journal of Integrative Medicine. 2017;9:1-8
  28. Jahangir M, ILYAS S, Mazhar F, Abbasi MA, Usman MF, Rehman J, et al. Antimicrobial and Antioxidant Potential of Astragalus psilocentros. Asian Journal of Chemistry. 2013;25(1)
  29. Albayrak S, Kaya O. Antioxidant and antimicrobial activities of four Astragalus species growing wild in Turkey. Turkish Journal of Biochemistry. 2018;43(4):425-34
  30. Anwar F, Abbas A, Mehmood T, Gilani AH, Rehman Nu. Mentha: A genus rich in vital nutra‐pharmaceuticals—A review. Phytotherapy Research. 2019;33(10):2548-70
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