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Ayatollahi Mousavi, Seyyed Amin, Mohammadian, Mahla, Hosseini Nave, Hossein, Mohseni, Parvin, Kalantar-Neyestanaki, Davood. (1404). Determination of ESBLs, pAmpC-beta-lactamase genes, and plasmid replicon types among Shigella species from different cities in Iran. سامانه مدیریت نشریات علمی, 80(4), 897-906. doi: 10.32592/ARI.2025.80.4.897
Seyyed Amin Ayatollahi Mousavi; Mahla Mohammadian; Hossein Hosseini Nave; Parvin Mohseni; Davood Kalantar-Neyestanaki. "Determination of ESBLs, pAmpC-beta-lactamase genes, and plasmid replicon types among Shigella species from different cities in Iran". سامانه مدیریت نشریات علمی, 80, 4, 1404, 897-906. doi: 10.32592/ARI.2025.80.4.897
Ayatollahi Mousavi, Seyyed Amin, Mohammadian, Mahla, Hosseini Nave, Hossein, Mohseni, Parvin, Kalantar-Neyestanaki, Davood. (1404). 'Determination of ESBLs, pAmpC-beta-lactamase genes, and plasmid replicon types among Shigella species from different cities in Iran', سامانه مدیریت نشریات علمی, 80(4), pp. 897-906. doi: 10.32592/ARI.2025.80.4.897
Ayatollahi Mousavi, Seyyed Amin, Mohammadian, Mahla, Hosseini Nave, Hossein, Mohseni, Parvin, Kalantar-Neyestanaki, Davood. Determination of ESBLs, pAmpC-beta-lactamase genes, and plasmid replicon types among Shigella species from different cities in Iran. سامانه مدیریت نشریات علمی, 1404; 80(4): 897-906. doi: 10.32592/ARI.2025.80.4.897

Determination of ESBLs, pAmpC-beta-lactamase genes, and plasmid replicon types among Shigella species from different cities in Iran

Archives of Razi Institute
مقاله 9، دوره 80، شماره 4، مهر و آبان 2025، صفحه 897-906    اصل مقاله (510.35 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.4.897
نویسندگان
Seyyed Amin Ayatollahi Mousavi1، 2؛ Mahla Mohammadian3؛ Hossein Hosseini Nave4، 5؛ Parvin Mohseni6؛ Davood Kalantar-Neyestanaki* 1، 5
1Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
2Department of Medical Mycology and Parasitology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
3Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
4Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran
5Department of Medical Microbiology (Bacteriology and virology), Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
6Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
چکیده
Shigella species (spp) are the common gram-negative bacilli isolated from patients with diarrhea. Infection treatment of these genus of bacteria due to increasing resistance to antibiotic agents remains a global challenge. Herein, we determined the frequency of ESBLs, plasmid-mediated AmpC-beta-lactamase (pAmpC) genes, and plasmid replicon types in 210 clinical isolates of Shigella spp from different cities in Iran. The antibacterial susceptibility of isolates to antibiotic agents and ESBLs production were determined according to the Clinical & Laboratory Standards Institute (CLSI) recommendations. ESBLs, pAmpC genes, and plasmid replicon types of isolates were detected using PCR and multiplex PCR methods. The highest rate of antibiotic resistance was observed to trimethoprim-sulfamethoxazole and the lowest rate of resistance was observed to cefoxitin. Fifty-four percent of the isolates were considered as ESBL-producers. Beta-lactamase genes including blaCTX-M, blaTEM, and blaDHA were detected in 93 (44%), 84 (40%), and 3 (1.4%) of isolates, respectively. Ten various plasmid replicon types including I1-Iγ, K, W, FIB, Y, P, FIC, FIA, HI1, and B/O were identified among the isolates. The study sheds light on the persistent challenges posed by multidrug-resistant (MDR) shigellosis to public health in different regions in Iran. Despite advancements in hygiene practices, the prevalence and population composition of Shigella species have remained largely unchanged. Also, the spread of beta-lactamase genes and various plasmid replicon types are increasing among the Shigella spp in our country which can be challenging to treat their infection and more efficient strategies, and monitoring should be considered to prevent the spread of them.
کلیدواژه‌ها
Shigella species؛ ESBLs؛ AmpC beta-lactamase؛ Plasmid replicon types
سایر فایل های مرتبط با مقاله
مراجع
  1. Organization WH. Microbiological Risk Assessment–Guidance for food. Food & Agriculture Org. 2021:36.
  2. Moxley RA. Enterobacteriaceae: Shigella. Vet Microbiol. 2022;100-7.
  3. Williams PCM, Berkley JA. Guidelines for the treatment of dysentery (shigellosis): a systematic review of the evidence. Paediatr Int Child Health. 2018;38(sup1):S50–65.
  4. Wang Y, Ma Q, Hao R, Zhang Q, Yao S, Han J, et al. Antimicrobial resistance and genetic characterization of Shigella spp. in Shanxi Province, China, during 2006-2016. BMC Microbiol. 2019;19:1-11.
  5. CLSI Performance Standards for Antimicrobial Susceptibility Testing. 33rd ed. CLSI supplement M100. Clinical and Laboratory Standards Institute; 2023.
  6. Pérez-Pérez FJ, Hanson ND. Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol. 2002;40(6):2153-62.
  7. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005;63(3):219-28.
  8. Nikfar R, Shamsizadeh A, Darbor M, Khaghani S, Moghaddam M. A study of prevalence of Shigella species and antimicrobial resistance patterns in paediatric medical center, Ahvaz, Iran. Iran J Microbiol. 2017;9(5):277.
  9. Moosavian M, Ghaderiyan GH, Shahin M, Navidifar T. First investigation of the presence of SPATE genes in Shigella species isolated from children with diarrhea infection in Ahvaz, southwest Iran. Infect Drug Resist. 2019;10(12):795-804.
  10. Qu M, Zhang X, Liu G, Huang Y, Jia L, Liang W, et al. An eight-year study of Shigella species in Beijing, China: serodiversity, virulence genes, and antimicrobial resistance. J Infect Dev Ctries. 2014;8(7):904–8.
  11. Thompson CN, Duy PT, Baker S. The rising dominance of Shigella sonnei: an intercontinental shift in the etiology of bacillary dysentery. PLoS Negl Trop Dis. 2015;9(6):e0003708.
  12. Ranjbar R, Ghazi FM, Farshad S, Giammanco GM, Aleo A, Owlia P, et al. The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran. Iran J Microbiol. 2013;5(2):108.
  13. Sabour S, Teimourpour A, Mohammadshahi J, Peeridogaheh H, Teimourpour R, Azimi T, et al. Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran. Mol Cell Pediatr. 2022;9(1):19.
  14. Soltan Dallal MM, Ranjbar R, Yaghoubi S, Rajabi Z, Aminharati F, Adeli Behrooz H. Molecular epidemiology and genetic characterization of Shigella in pediatric patients in Iran. Le Infez Med. 2018;26(4):321-8.
  15. Andres P, Petroni A, Faccone D, Pasterán F, Melano R, Rapoport M, et al. Extended-spectrum β-lactamases in Shigella flexneri from Argentina: first report of TOHO-1 outside Japan. Int J Antimicrob Agents. 2005;25(6):501-7.
  16. Xiong Z, Li T, Xu Y, Li J. Detection of CTX-M-14 extended-spectrum β-lactamase in Shigella sonnei isolates from China. J Infect. 2007;55(5):e125–8.
  17. Alici O, Açikgöz ZC, Göçer S, Gamberzade S, Karahocagil MK. Short communication: prevalence of extended spectrum beta-lactamases in gram negative rods: data of 2001-2004 period. Mikrobiyol Bul. 2006;40(4):355–61.
  18. Zhang J, Jin H, Hu J, Yuan Z, Shi W, Yang X, et al. Antimicrobial resistance of Shigella spp. from humans in Shanghai, China, 2004–2011. Diagn Microbiol Infect Dis. 2014;78(3):282–6.
  19. Qu F, Bao C, Chen S, Cui E, Guo T, Wang H, et al. Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007. Diagn Microbiol Infect Dis. 2012;74(2):166–70.
  20. Gu B, Cao Y, Pan S, Zhuang L, Yu R, Peng Z, et al. Comparison of the prevalence and changing resistance to nalidixic acid and ciprofloxacin of Shigella between Europe–America and Asia–Africa from 1998 to 2009. Int J Antimicrob Agents. 2012;40(1):9-17.
  21. Jafari F, Garcia-gil LJ, Salmanzadeh-ahrabi S, Shokrzadeh L. Diagnosis and prevalence of enteropathogenic bacteria in children less than 5 years of age with acute diarrhea in Tehran children ’ s hospitals. 2009;70.
  22. Mostafavi N, Bighamian M, Mobasherizade S, Kelishadi R. Resistance of Shigella strains to extended-spectrum cephalosporins in Isfahan province. Med J Islam Repub Iran. 2016;17(30):428.
  23. Gebrekidan A, Dejene TA, Kahsay G, Wasihun AG. Prevalence and antimicrobial susceptibility patterns of Shigella among acute diarrheal outpatients in Mekelle hospital, Northern Ethiopia. BMC Res Notes. 2015;8:1-7.
  24. Jamshidi AA, Matbooei A. Shigella spp frequency, serotyping and antibiotic resistance pattern in acute diarrheic patients in Zanjan Shahid Beheshti Hospital, during 2003-2007. J Adv Med Biomed Res. 2008;16(62):77-84.
  25. Jomezadeh N, Babamoradi S, Kalantar E, Javaherizadeh H. Isolation and antibiotic susceptibility of Shigella species from stool samples among hospitalized children in Abadan, Iran. Gastroenterol Hepatol from bed to bench. 2014;7(4):218.
  26. Ruekit S, Wangchuk S, Dorji T, Tshering KP, Pootong P, Nobthai P, et al. Molecular characterization and PCR-based replicon typing of multidrug resistant Shigella sonnei isolates from an outbreak in Thimphu, Bhutan. BMC Res Notes. 2014;7:1-9.
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