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Shahsavandi, Shahla, Nasr Isfahani, Hadi, Hariri, Amir Ali, Sharifnia, Zarrin, Soleimani, Sina, Moradi, Abolfazl. (1403). Safflower-derived cationic lipid nanoparticles: potential impact on the delivery of SARS-CoV-2 mRNA transcripts. سامانه مدیریت نشریات علمی, 79(6), 1217-1226. doi: 10.32592/ARI.2024.79.6.1217
Shahla Shahsavandi; Hadi Nasr Isfahani; Amir Ali Hariri; Zarrin Sharifnia; Sina Soleimani; Abolfazl Moradi. "Safflower-derived cationic lipid nanoparticles: potential impact on the delivery of SARS-CoV-2 mRNA transcripts". سامانه مدیریت نشریات علمی, 79, 6, 1403, 1217-1226. doi: 10.32592/ARI.2024.79.6.1217
Shahsavandi, Shahla, Nasr Isfahani, Hadi, Hariri, Amir Ali, Sharifnia, Zarrin, Soleimani, Sina, Moradi, Abolfazl. (1403). 'Safflower-derived cationic lipid nanoparticles: potential impact on the delivery of SARS-CoV-2 mRNA transcripts', سامانه مدیریت نشریات علمی, 79(6), pp. 1217-1226. doi: 10.32592/ARI.2024.79.6.1217
Shahsavandi, Shahla, Nasr Isfahani, Hadi, Hariri, Amir Ali, Sharifnia, Zarrin, Soleimani, Sina, Moradi, Abolfazl. Safflower-derived cationic lipid nanoparticles: potential impact on the delivery of SARS-CoV-2 mRNA transcripts. سامانه مدیریت نشریات علمی, 1403; 79(6): 1217-1226. doi: 10.32592/ARI.2024.79.6.1217

Safflower-derived cationic lipid nanoparticles: potential impact on the delivery of SARS-CoV-2 mRNA transcripts

Archives of Razi Institute
مقاله 11، دوره 79، شماره 6، بهمن و اسفند 2024، صفحه 1217-1226    اصل مقاله (509.87 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.6.1217
نویسندگان
Shahla Shahsavandi* 1؛ Hadi Nasr Isfahani2؛ Amir Ali Hariri2؛ Zarrin Sharifnia2؛ Sina Soleimani3؛ Abolfazl Moradi2
1Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization, Karaj, Iran
2Biotechnology Department, Behyaar Sanaat Sepahan Company, Isfahan, Iran
3Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
چکیده
The COVID-19 pandemic has significantly highlighted the successful application of lipid nanoparticles (LNPs) as an advanced platform for mRNA vaccine delivery. Ionizable lipid is the main component for complexing the mRNA in LNP formulation and in vivo delivery. In the first step of this study, we used the native safflower oilseed to prepare dilinoleyl alcohol. Then the cationic lipid (MC3) was synthesized by mixing the alcohol with dimethylamino butyric acid. Safflower-derived MC3 was applied to formulate an LNP vector with standard composition. The efficiency of the synthetic cationic lipid was evaluated for delivering an mRNA-based vaccine encoding the receptor-binding domain (RBD) of SARS-CoV-2. The produced mRNA-LNP vaccine candidate was evaluated in terms of size, morphology, mRNA encapsulation efficiency, apparent pKa, and stability for nucleic acid delivery. Cellular uptake was determined by measuring the percentage of GFP expression, and cytotoxicity was assayed using MTT. The MC3 formation was confirmed by the NMR spectra and used as a cationic lipid in LNP formulation. The obtained LNPs had positively charged and appropriate particle sizes (~80 nm) to confer proper encapsulation efficiency for mRNA delivery and stability. The LNPs were shown to be effective in the transfection of mRNA transcripts into HEK293T cells. A high level (72.34%) of cellular uptake was determined by measuring the percentage of GFP expression. The cytotoxicity assay using MTT showed that both LNP and mRNA-LNP were non-toxic to cells. The data show the potential of the proposed cationic lipid in the formulation of LNP applicable to the mRNA delivery system. The efficiency of mRNA delivery could have been attributed to the safflower-derived cationic lipid.
کلیدواژه‌ها
Cationic lipid؛ Lipid-based nanoparticles؛ Safflower oil؛ mRNA vaccine؛ SARS-CoV-2
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مراجع
  1. Thi TT, Suys EJ, Lee JS, Nguyen DH, Park KD, Truong NP. Lipid-based nanoparticles in the clinic and clinical trials: from cancer nanomedicine to COVID-19 vaccines. Vaccines. 2021;9(4):359.
  2. Midoux P, Pichon C. Lipid-based mRNA vaccine delivery systems. Expert Rev Vaccines. 2015;14(2):221-234.
  3. Hu B, Zhong L, Weng Y, Peng L, Huang Y, Zhao Y, Liang XJ. Therapeutic siRNA: state of the art. Signal Transduct Target Ther. 2020; 5(1):101.
  4. Qin S, Tang X, Chen Y, Chen K, Fan N, Xiao W, Zheng Q, Li G, Teng Y, Wu M, Song X. mRNA-based therapeutics: powerful and versatile tools to combat diseases. Signal Transduct Target Ther. 2022;7(1):166.
  5. Reichmuth AM, Oberli MA, Jaklenec A, Langer R, Blankschtein D. mRNA vaccine delivery using lipid nanoparticles. Ther Deliv. 2016;7(5):319-334.
  6. Wilson B, Geetha KM. Lipid nanoparticles in the development of mRNA vaccines for COVID-19. J Drug Deliv Sci Technol. 2022;74:103553.
  7. Wolff JA, Malone RW, Williams P, Chong W, Acsadi G, Jani A, Felgner PL. Direct gene transfer into mouse muscle in vivo. Science. 1990;247(4949):1465-1468.
  8. Kowalski PS, Rudra A, Miao L, Anderson DG. Delivering the messenger: advances in technologies for therapeutic mRNA delivery. Mol Ther. 2019; 27(4):710-728.
  9. Uchida S, Perche F, Pichon C, Cabral H. Nanomedicine-based approaches for mRNA delivery. Mol Pharm. 2020;17(10):3654-3684.
  10. Andries O, Mc Cafferty S, De Smedt SC, Weiss R, Sanders NN, Kitada T. N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J Control Release. 2015;217:337-344.
  11. Pardi N, Hogan MJ, Porter FW, Weissman D. mRNA vaccines—a new era in vaccinology. Nat Rev Drug Disco. 2018; 17(4):261-279.
  12. Eygeris Y, Gupta M, Kim J, Sahay G. Chemistry of lipid nanoparticles for RNA delivery. Acc Chem Res. 2021;55(1):2-12.
  13. Carrasco MJ, Alishetty S, Alameh MG, Said H, Wright L, Paige M, Soliman O, Weissman D, Cleveland IV TE, Grishaev A, Buschmann MD. Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration. Commun Biol. 2021;4(1):956.
  14. Malburet C, Leclercq L, Cotte JF, Thiebaud J, Bazin E, Garinot M, Cottet H. Size and charge characterization of lipid nanoparticles for mRNA vaccines. Anal Chem. 2022;94(11):4677-4685.
  15. Hajj KA, Ball RL, Deluty SB, Singh SR, Strelkova D, Knapp CM, Whitehead KA. Branched‐tail lipid nanoparticles potently deliver mRNA in vivo due to enhanced ionization at endosomal pH. Small. 2019;15(6):1805097.
  16. Zhang Y, Sun C, Wang C, Jankovic KE, Dong Y. Lipids and lipid derivatives for RNA delivery. Chem Rev. 2021; 121(20):12181-12277.
  17. Rietwyk S, Peer D. Next-generation lipids in RNA interference therapeutics. ACS Nano. 2017;11(8):7572-7586.
  18. Katkade MB, Syed HM, Andhale RR, Sontakke MD. Fatty acid profile and quality assessment of safflower (Carthamus tinctorius) oil. JPP. 2018;7(2): 3581-3585.
  19. Shahsavandi S, Hariri AA. SARS-CoV-2 Variant-Specific mRNA Vaccine: Pros and Cons. Viral Immunol. 2023;36(3):186-202.
  20. Patel R, Kaki M, Potluri VS, Kahar P, Khanna D. A comprehensive review of SARS-CoV-2 vaccines: Pfizer, moderna & Johnson & Johnson. Human Vaccin Immunother. 2022;18(1):2002083.
  21. Chen L, Simpson JD, Fuchs AV, Rolfe BE, Thurecht KJ. Effects of surface charge of hyperbranched polymers on cytotoxicity, dynamic cellular uptake and localization, hemotoxicity, and pharmacokinetics in mice. Mol Pharm. 2017;14(12):4485-4497.
  22. Zhang Y, Sun C, Wang C, Jankovic KE, Dong Y. Lipids and lipid derivatives for RNA delivery. Chem Rev. 2021;121(20):12181-12277.
  23. Hou X, Zaks T, Langer R, Dong Y. Lipid nanoparticles for mRNA delivery. Nat Rev Mater. 2021;6(12):1078-1094.
  24. Carrasco MJ, Alishetty S, Alameh MG, Said H, Wright L, Paige M, Soliman O, Weissman D, Cleveland TE 4th, Grishaev A, Buschmann MD. Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration. Commun Biol. 2021;4(1):956.
  25. Schlich M, Palomba R, Costabile G, Mizrahy S, Pannuzzo M, Peer D, Decuzzi P. Cytosolic delivery of nucleic acids: The case of ionizable lipid nanoparticles. Bioeng Transl Med. 2021;6(2):e10213.
  26. Patel P, Ibrahim NM, Cheng K. The Importance of Apparent pKa in the Development of Nanoparticles Encapsulating siRNA and mRNA. Trends Pharmacol Sci. 2021;42(6):448-460.
  27. Zhang C, Ma Y, Zhang J, Kuo JC, Zhang Z, Xie H, Zhu J, Liu T. Modification of lipid-based nanoparticles: an efficient delivery system for nucleic acid-based immunotherapy. Molecules. 2022;27(6):1943.
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