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Shahkarami, Mohammad kazem, Lotfi, Mohsen, Salimi, Vahid, Namavari, Mohammad Mehdi, Soleimani, Sina, Yousefi, Ali Reza. (1403). Sensitivity of a Novel Lizard-Derived Cell Line (Z1) to Measles, Rubella and Respiratory Syncytial Viruses.. سامانه مدیریت نشریات علمی, 80(1), 193-200. doi: 10.32592/ARI.2025.80.1.193
Mohammad kazem Shahkarami; Mohsen Lotfi; Vahid Salimi; Mohammad Mehdi Namavari; Sina Soleimani; Ali Reza Yousefi. "Sensitivity of a Novel Lizard-Derived Cell Line (Z1) to Measles, Rubella and Respiratory Syncytial Viruses.". سامانه مدیریت نشریات علمی, 80, 1, 1403, 193-200. doi: 10.32592/ARI.2025.80.1.193
Shahkarami, Mohammad kazem, Lotfi, Mohsen, Salimi, Vahid, Namavari, Mohammad Mehdi, Soleimani, Sina, Yousefi, Ali Reza. (1403). 'Sensitivity of a Novel Lizard-Derived Cell Line (Z1) to Measles, Rubella and Respiratory Syncytial Viruses.', سامانه مدیریت نشریات علمی, 80(1), pp. 193-200. doi: 10.32592/ARI.2025.80.1.193
Shahkarami, Mohammad kazem, Lotfi, Mohsen, Salimi, Vahid, Namavari, Mohammad Mehdi, Soleimani, Sina, Yousefi, Ali Reza. Sensitivity of a Novel Lizard-Derived Cell Line (Z1) to Measles, Rubella and Respiratory Syncytial Viruses.. سامانه مدیریت نشریات علمی, 1403; 80(1): 193-200. doi: 10.32592/ARI.2025.80.1.193

Sensitivity of a Novel Lizard-Derived Cell Line (Z1) to Measles, Rubella and Respiratory Syncytial Viruses.

Archives of Razi Institute
مقاله 23، دوره 80، شماره 1، فروردین و اردیبهشت 2025، صفحه 193-200    اصل مقاله (446.85 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.1.193
نویسندگان
Mohammad kazem Shahkarami* 1؛ Mohsen Lotfi2؛ Vahid Salimi3؛ Mohammad Mehdi Namavari4؛ Sina Soleimani2؛ Ali Reza Yousefi5
1Human Viral Vaccines department, Razi vaccine and Serum Research Institute, Agricultural research, education and extension organization (Areeo), Karaj, Iran.
2Quality Control department, Razi vaccine and Serum Research Institute, Agricultural research, education and extension organization (Areeo), Karaj, Iran.
3Virology department, School of public health, Tehran University of Medical Sciences, Tehran, Iran.
4Razi vaccine and Serum Research Institute, Shiraz branch, Agricultural research, education and extension organization (Areeo), Shiraz, Iran.
5Department of Pathology and Experimental Animals, Razi vaccine and Serum Research Institute, Agricultural research, education and extension organization (AREEO), Karaj, Iran.
چکیده
This study aimed to investigate the sensitivity of the Z1 cell line to Measles, Rubella and Respiratory Syncytial Viruses, and to follow up on the formation of subsequent cytopathic effects for evaluating the potential of the Z1 cell line as a suitable alternative for easier isolation, and identification of these viruses, and even quality control tests such as potency tests and production of biological products such as diagnostic antigens. The Z1 cell lines were prepared in disposable cell culture flasks and were inoculated with Measles, Rubella, and Respiratory Syncytial Viruses at defined multiplicity of infection. Vero-WHO, Rabbit Kidney-13 (RK-13), and Hep-2 cell lines were also prepared as standard cell substrates and were inoculated with Measles, Rubella and Respiratory Syncytial Viruses, respectively. The quality and quantity of CPE formation as the microscopic signs of viral replication in the test and control cell lines were daily observed. Samples were taken and the titer of progeny viruses was calculated to compare the viral yield. An interference test using the Vesicular Stomatitis Virus was also performed to confirm the replication of Rubella in the Rubella-inoculated cell cultures. In the case of Measles Virus there was a significant difference between viral yield in Z1 and Vero-WHO cells. For Rubella Virus there was a significant differences in viral yield in Z1 and Vero-WHO cells. Despite these, there were no significant difference between viral yield, when Respiratory Syncytial Virus inoculated to the Z1 and Vero-WHO cells. Collectively, the Z1 cell line could be considered a suitable alternative to Hep-2 for isolation and propagation of Respiratory Syncytial Virus, while it is relatively sensitive to Measles Virus. Despite this, the Z1 cell line could not be used as a cell substrate for Rubella Virus propagation.
کلیدواژه‌ها
Propagation؛ Permissive؛ Cell line؛ Lizard
عنوان مقاله [English]
حساسیت یک رده سلولی با منشاء خزنده (Z1) به ویروسهای سرخک، سرخجه و ویروس سینسی شیال تنفسی.
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مراجع
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  4. Mohammad Mehdi Namavari, Abollah Rahimian, Narges Mashkour, Inventor; Razi Vaccine and Serum Research Institute, assignee. Introduction of the cell series (1-6) as the first cell lines that are developed from home lizard (eyrtopodion scabrum). Iranian patent No. 67770, 2010.
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  9. George et al. Molecular aspects of the teratogenesis of rubella virus Suji George, Rajlakshmi Viswanathan and Gajanan N. Sapkal. Biol Res. 2019;52:47.
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