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بررسی مولکولی میکروفلور باکتریایی همراه و درون‌زی قارچ دکمه‌ای سفید Agaricus bisporus | ||
| آفات و بیماری های گیاهی | ||
| دوره 92، شماره 1 - شماره پیاپی 118، 1403، صفحه 91-106 اصل مقاله (704.84 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2024.365734.1517 | ||
| نویسندگان | ||
| الهام موسوی جعفری پور1؛ مژده دوستی2؛ رضا صادقی3؛ نرگس فلاحی چرخابی* 4؛ مسعود احمدزاده5 | ||
| 1گروه حشره شناسی و بیماریهای گیاهی، دانشکده فناوری کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| 2گروه حشره شناسی و بیماریهای گیاهی، دانشکده فناوری کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران شرکت راژفدک مهرشهر، | ||
| 3پردیس ابوریحان دانشگاه تهران | ||
| 4Blvd. Imam Reza | ||
| 5کرج پردیس کشاورزی و منابع طبیعی دانشگاه تهران گروه گیاه پزشکی | ||
| چکیده | ||
| قارچ خوراکی دکمه‌ای سفید (Agaricus bisporus) به دلیل داشتن ارزش غذایی بالا و خـواص دارویـی متعـدد، جایگاه ویژه‌ای را در سبد غذایی مردم جهان بـه خـود اختصـاص داده است، از این رو کشت این منبع غذایی مفید در جهان هر روز گسترده‌تر می‌شود. در بررسی عوامل باکتریایی ایجاد‌کننده بیماری لکهقهوهای روی قارچ خوراکی در استان البرز از قارچ‌های دارای علائم بیماری و برای جداسازی عوامل باکتریایی بیوکنترل علیه بیماری لکهقهوهای از قارچ های سالم نمونهبرداری شد. از نمونههای آلوده 61 جدایه باکتریایی به دست آمد، نتایج نشان داد که 19 جدایه در قارچ خوراکی بیماری زا بودند و روی قارچ خوراکی علائم تغییر رنگ در حاشیه های جانبی و سطح کلاهک، لکه های وسیع، آبکی و فرورفته و لکه های ریز سوزنی شکل و قهوهای روی کلاهک و نکروزه بخش میانی و پایه قارچ خوراکی ایجاد کردند. از قارچ های سالم 9 جدایه باکتری جداسازی شد که روی قارچ خوراکی بیماری‌زا نبودند و چهار جدایه به عنوان جدایه های درون‌زی منتخب به منظور ارزیابی توانایی مکانیسم های بازدارندگی (تولید هورمون اکسین سیدروفور و تولید آنزیم های فیتاز، پروتئاز، لیپاز، لسیتیناز، سلولاز و کیتیناز) بررسی شدند. آزمونهای بیوشیمیایی و مولکولی برای شناسایی باکتری‌های بیماری زا و درون‌زی انجام شد. بر اساس بررسی ترادفهای نوکلوئوتیدی ژن16S rRNA باکتری‌های بیمارگر در جنس های بیمارگر Pseudomonas،Chryseobacterium، Ewingella، Brucella وKlebsiella و باکتری‌های غیربیمارگر درون‌زی در گونه‌های Bacillus velezensis، Kocuria rhizophila، Bacillus altitudinis,Bacillus wiedmannii قرار گرفتند. | ||
| کلیدواژه‌ها | ||
| قارچ خوراکی؛ لکه قهوهای؛ Pseudomonas tolaasii | ||
| عنوان مقاله [English] | ||
| Molecular analysis of the bacterial microflora associated with and endofungal of Agaricus bisprus | ||
| نویسندگان [English] | ||
| Elham Mousavi Jafaripour1؛ Mozhdeh Doosti2؛ Reza Sadeghi3؛ Nargues Falahi Charkhabi4؛ Masoud Ahmadzadeh5 | ||
| 1Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran | ||
| 2Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran Research and Development Manager, Razh fadak company, Mehrshahr, Karaj, | ||
| 3, Department of Entomology and Plant Pathology, College of Agricultural Technology, Univers, Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Te | ||
| 4Blvd. Imam Reza | ||
| 5Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran | ||
| چکیده [English] | ||
| White button mushroom (Agaricus bisporus) has a special place in the food basket worldwide because of its high nutritional value and medicinal properties. To identify bacterial species associated with brown spot disease of edible mushrooms symptomatic samples were collected from Alborz province farms. Moreover, healthy mushrooms were sampled to isolate biocontrol bacterial agents. Sixty-one bacterial isolates were isolated from symptomatic samples among which 19 isolates induced wide, watery and sunken spots on the surface of the cap and necrosis of the middle part and the base of edible mushroom. Nine non-pathogenic isolates were isolated from healthy mushrooms among which four isolated were selected for auxin hormone, siderophore and production of phytase, protease, lipase, lecithinase, cellulase and chitinase enzymes production assays. Biochemical and molecular tests were performed to identify pathogenic and endofungal bacteria. Based on the 16S rDNA sequence analysis isolates were classified into the pathogenic genera including Pseudomonas, Chryseobacterium, Ewingella, Brucella and Klebsiella and endofungal species including Bacillus velezensis, Kocuria rhizophila, Bacillus wiedmannii. | ||
| کلیدواژه‌ها [English] | ||
| Brown spot disease, edible mushroom, Pseudomonas tolaasi | ||
| مراجع | ||
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