1Genetics, Biotechnology and Bioresources valorization Laboratory Department of Nature and Life Sciences, Faculty of Exact Sciences and Sciences of Nature and Life, Mohamed Kheider University, Biskra, Algeria.
2Department of Agriculture sciences, university of Biskra Algeria
3Laboratory of Genetic, Biotechnology and Valorization of Bioresources (LGBVB), University of Biskra, Algeria)
4Applied Neuro-endocrinology Laboratory, Department of Nature and Life Sciences, Faculty of Exact Sciences and Sciences of Nature and Life, Badji Mokhtar University, Annaba, Algeria
چکیده
The objective of this study is to determining mainly, the toxicity of Bacillus thuringiensis svar. kurstaki (Bt) on the first larval instar of Ectomyelois ceratoniae and also to study its deferred effect on other biological parameters such as the development and reproduction of this pest. We paired the treated larvae, The concentrations used are: 0,25 g/L (250 ppm), 0,5 g/1L (500 ppm), 1 g/L (1000 ppm), 1,5 g/L (1500 ppm) and 2 g/L (2000 ppm) (6 pairs for each dose used), and placed 6 control pairs on separate Petri dishes to count the number of eggs laid. We then tallied the number of eggs that hatched after their incubation. For Bt svar. kurstaki, the variable being measured is the rate of larval mortality. The results showed that there was a strong and positive link between the doses used and the adjusted mortality of the larvae across a range of bioinsecticide exposure times, the five Bt svar. kurstaki concentrations used had generated a corrected mortality of E. ceratoniae first instar larvae which varied between a minimum of 50.78% and a maximum of 97.92%. Bacillus thuringiensis svar. kurstaki becomes more and more toxic each time the larvae were exposed to the biopesticide. Thus, the LC50 of Bt svar. kurstaki for E. ceratoniae larvae, calculated at the concentrations of 250, 500, 1000, 1500, 2000 ppm were inversely proportional to the different lethal times. On the other hand, the Bt treatment decreased female insects' reproductive rate and egg viability. Therefore, the BT had a negative influence on the growth and reproduction parameters of E. ceratoniae.
Bedjaoui H, Benbouza H. Assessment of phenotypic diversity of local Algerian date palm (Phoenix dactylifera L.) cultivars. Journal of the Saudi Society of Agricultural Sciences. 2020;19(1):65-75.
CHAABAN SB, MNAFFED A, MAHJOUBI K, BEN JM. Efficacy of essential oils to control the carob moth, Ectomyelois Ceratoniae Zeller (Lepidoptera: Pyralidae). International Journal of Agriculture Innovations and Research. 2019;7(4):2319-1473.
Peyrovi M, Goldansaz S, Jahromi KT. Using Ferula assafoetida essential oil as adult carob moth repellent in Qom pomegranate orchards (Iran). African Journal of Biotechnology. 2011;10(3):380-5.
Jouve P, Loussert R, Mouradi H, editors. La lutte contre la dégradation des palmeraies dans les oasis de la région de Tata (Maroc). Colloque international; 2006.
Ben Saad A. Evolution des systèmes de production oasiens dans le contexte de désengagement de l’état. Cas des oasis du grand Gabes Manuel gouvernance foncière et usage des ressources naturelles FONCIMED INRA. 2010.
Bazzi L. Etude de la persistance de quelques pesticides dans la culture de l’haricot vert dans la région de Sous Massa. 2010.
Zohra BF, Mahmoud Y, Farid B, Bahia D-M. Activité biologique d'un biopesticide le Green muscle sur le tégument du criquet pélerin Schistocerca gregaria (Forskål, 1775)(Orthoptera, Acrididae). Nature & Technology. 2012(6):51.
Bélanger A, Musabyimana T. Le Neem contre les insectes et les maladies. Journées Horticoles au Canada en. 2005.
Abdullah MA. Toxicological and histopathological studies of Boxus chinensis oil and precocene II on larvae of the red palm weevil Rynchophorus ferrugineus (Oliver)(Coleoptera: Curculionidae). Egyptian Academic Journal of Biological Sciences A, Entomology. 2009;2(2):45-54.
Richard I. Les pesticides et la perte de biodiversité. Comment l’usage intensif des pesticides affecte la faune et la flore sauvage et la diversité des espèces, Pesticide Action Network Europe, Belgium. 2010:p3.
Mediouni J, Dhouibi M. Mass-rearing and field performance of irradiated carob moth Ectomyelois ceratoniae in Tunisia. Area-wide control of insect pests: from research to field implementation: Springer; 2007. p. 265-73.
Dhouibi M. Bacillus thuringiensis Kurstaki contre la pyrale des dattes Ectomyelois ceratoniae Zeller (Lepidoptera Pyralidae). Tunis, Tunisie, INAT, 54 p, document technique. 1993.
Culver J, Vienna V, BURGESS PA. A method of computing the effectiveness of an insecticide. Journal of economic entomology. 1925;18:265.
Finney D. Probit analysis, Cambridge University Press. Cambridge, UK. 1971.
Swaroop S, Satya S. Statistical methods in malaria eradication. 1966.
HABBACHI W. Etude des Blattellidae (Dictyoptera): essais toxicologiques, synergie et résistance aux insecticides et aux biopesticides: Université de Annaba-Badji Mokhtar; 2013.
Chaufaux J. Utilisation de biopesticides contre les ravageurs des cultures: le point sur Bacillus thuringiensis. 1995.
LAMBRET P. Dynamique de populations d’adultes de Lestes macrostigma (Eversmann, 1836) et implications pour son suivi: l’exemple de la Camargue.
Lereclus D, Chaufaux J. Etat actuel de la lutte biologique a l'aide de Bacillus thuringiensis: ce bioinsecticide permettra t-il demain d'atteindre le doryphore? 1986.
Drummond J, Pinnock DE. Host spectrum of Bacillus thuringiensis. Agriculture, ecosystems & environment. 1994;49(1):15-9.
Abid I, Laghfiri M, Bouamri R, Aleya L, Bourioug M. Integrated pest management (IPM) for Ectomyelois ceratoniae on date palm. Current Opinion in Environmental Science & Health. 2021;19:100219.
Fernández-Chapa D, Ramírez-Villalobos J, Galán-Wong L. thuringiensis: An Overview. Protecting rice grains in the post-genomic era. 2019;2:183.
آمار
تعداد مشاهده مقاله: 803
تعداد دریافت فایل اصل مقاله: 484
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب
