The effect of plant growth-promoting bacteria on Cereal cyst nematode (Heterodera avenae) under laboratory and greenhouse conditions | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 11, Issue 1, August 2023, Pages 83-96 PDF (918.94 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2024.365659.365 | ||
| Authors | ||
| Mohhamad Bazgir1; hossein mirzaei najafgholi* 2; Kourosh Azizi2; Neda Rouhani3; mohammad Reza alymanesh4 | ||
| 1M.Sc student, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
| 2Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran. | ||
| 3PhD graduated, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 4Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
| Abstract | ||
| One of the damaging agents for wheat is the cereal cyst nematode (Heterodera avenae). Plant Growth Promoting Bacteria (PGPR) are a group of bacteria that have beneficial properties for plant growth, including iron chelation, siderophore production, phosphate dissolution, nitrogen fixation, as well as the production of protease and chitinase enzymes. These bacteria can also be used as biological control agents. In this study, wheat roots showing suspicious symptoms of cereal cyst nematode were collected from the Dezful county in Khuzestan province during 2022-2023. After initial investigations, nematode identification and purification was done. According to the morphometric and morphological characteristics of the larvae and cysts, especially its cone top, H. avenae was diagnosed. the effect of seven native growth-stimulating bacterial strains on egg hatching rate and mortality of cereal cyst nematode larvae in the Petri dish was investigated in the laboratory. The effect of four selected bacterial strains with sulfur treatment and Nematex EC nematicide was investigated in the greenhouse against grain cyst nematode. The results showed that in laboratory conditions, the bacterial strains were able to reduce the egg hatching rate by 62% on average. Bacillus pumilus bacteria caused the greatest decrease in egg hatching by 73%. The percentage of mortality of larvae in the laboratory test was also shown to be 63% on average, and the treatment of B. pumilus bacteria showed the highest percentage of mortality with 70%. The results of the greenhouse investigations showed that the treatments involving sulfur-healthy plants, sulfur-nematode, and the B. pumilus and P. fluorescens bacteria were most effective in promoting healthy plant growth and controlling nematode infestation. These treatments resulted in significant improvements in plant height, as well as fresh and dry plant and root weights. Additionally, the greatest reduction in the number of cysts was observed in the B. pumilus treatment, with an average of 49 ± 1 cysts. When examining the number of eggs within the cysts, the most substantial decrease was observed in the Nematex EC and B. pumilus bacteria treatments, with 67 ± 1 and 111 ± 2 eggs, respectively. Furthermore, in the assessment of the number of live larvae in the pot, the greatest decrease in the number of larvae was observed in the Nematex treatment, with 87 ± 1. . | ||
| Keywords | ||
| Nematode; Biological Control; Wheat; Bacteria | ||
| References | ||
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Agriculture statistics, 2022. Ministry of Jihad-e- Agriculture, Economic and planting assistance information and communication technology center. First volume, crop products, pp.15-16. (In Persian with English summary). Ahmed, S., Yue, L., Liu, Q. & Jian, H. 2018. Bio-control potential of Bacillus isolates against cereal cyst nematode (Heterodera avenae), Pakistan Journal of Nematology, 36(2): 163-176. Ahmed, S., Yue, L., Liu, Q. & Jian, H. 2019. Bacillus cereus a potential strain infested cereal cyst nematode (Heterodera avenae), Pakistan Journal of Nematology, 37(1): 53-61. Dawabah, A.A., Al-Hazmi, A.S., Al-Rehiayani, S.M., Abdel-Mawgoo, A.L., Motawei, M.I., Al-Otayk, S., Sadder, M.T., Elgorban, A.M., Migdadi, H.M. & Moustafa, K.A. 2012. Morphological and molecular characterization of cereal cyst nematode (Heterodera avenae) populations from arid environments, Australian Journal of Crop Science, 6(6): 970-979. De Grisse, A.T. 1969. Redescription ou modification dequelques techniques utilisees dans l’etude des nematodes phytoparasitaires, Rijks fakulteit Land wetenschappen Gent, 34: 351-369. Jha, C.K. & Saraf, M. 2015. Plant growth promoting rhizobacteria (PGPR): a review, Journal of Agricultural Research and Development, 5: 108-119. Faostat, F.A.O. Food and Agriculture Organization of the United Nations-Statistic – Statistical Yearbook 2022 Fidler, J.H. & Bevan, W.J. 1963. density of cereal root eelworm (Heterodera avenae Woll) populations and their damege to the oat crop, Nematologica, 9(3): 412-420. Hasanvand, E., Darvishnia, M., Mirzaei-Najafgholi, H. & Pakbaz, S. 2023. Isolation and identification of plant growth-promoting bacteria from the rhizosphere of wheat and evaluation of their growth-promoting and biocontrol properties, Genetic Engineering and Biosafety Journal, 11(2): 230-242. Mirzaei-Najafgholi, H., Hasanvand, E., Pakbaz, S. & Rouhani, N. 2023. Investigating Effective Factors in Improving Plant Growth by Native Phosphate-dissolving Bacteria Isolated from Wheat, Journal of Genetic Resources, 9(2): 193-204. Nicol, J. & Rivoal, M. 2008. Global knowledge and its application for the integrated control and management of nematodes on wheat In: Integrated management and biocontrol of vegetable and grain crops nematodes, Springer Academic Publishing, Dordrecht, The Netherlands, 243-287. Nicol, J.M. 2002. Important nematode pests of cereals, In: Bread wheat: Improvement and production”, FAO Plant Production and Protection Series, Rome, Italy, 345-366. Rivoal, R. & Cook, R.1993. Nematode pests of cereals. In: Plant parasitic nematodes in temperate agriculture, CAB International, Wallingford, UK, 259-303. Shahid, A. 2017. Seed bacterization with pseudomonas isolates against wheat cyst nematode (Heterodera avenae)”, Bangladesh Journal of Botany, 46(3): 995-1000. Shewry, P.R. 2009. Wheat, Journal of Experimental Botany, 60(6): 1537–53. Webster, J.M. 1972. Economic Nematology, Acadmic Press, p. 563. Weller, D.M. & Cook, R.J. 1983. Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads, Phytopathology, 73: 463-469. Whitehead, A. & Hemming, J. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil, Annals of applied Biology, 55: 25-38. Zafar Handoo, A. 2002. A Key and Compendium to Species of the Heterodera avenae Group (Nematoda: Heteroderidae), Journal of Nematology, 34(3): 250–262. Zhang, J., Yonghui, L., Hongxia, Y., Bingjian, S. & Honglian, L. 2015. Biological control of the cereal cyst nematode (Heterodera filipjevi) by Achromobacter xylosoxidans isolate 09X01 and Bacillus cereus isolate09B18, Department of Plant Pathology, 7: 1–19. | ||
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