اخبار و اعلانات

 آمار

تعداد نشریات282
تعداد نشریات سازمان80
تعداد شماره‌ها3,208
تعداد مقالات35,560
تعداد مشاهده مقاله53,007,160
تعداد دریافت فایل اصل مقاله92,290,385
Mohamad Zen, Nur Izzati, Zaidan, Uswatun Hasanah, Abd. Gani, Siti Salwa, Shamsi, Suhaili, Hassan, Masriana, Marikkar, Nazrim. (1403). Exploring the Therapeutic Potential of Malaysian Stevia rebaudiana (Bertoni) Bertoni leaves essential oil: A Comprehensive Study on Cell Viability and Biological Effects for Topical Applications. سامانه مدیریت نشریات علمی, 13(3), 504-512. doi: 10.22034/jmpb.2024.361730.1541
Nur Izzati Mohamad Zen; Uswatun Hasanah Zaidan; Siti Salwa Abd. Gani; Suhaili Shamsi; Masriana Hassan; Nazrim Marikkar. "Exploring the Therapeutic Potential of Malaysian Stevia rebaudiana (Bertoni) Bertoni leaves essential oil: A Comprehensive Study on Cell Viability and Biological Effects for Topical Applications". سامانه مدیریت نشریات علمی, 13, 3, 1403, 504-512. doi: 10.22034/jmpb.2024.361730.1541
Mohamad Zen, Nur Izzati, Zaidan, Uswatun Hasanah, Abd. Gani, Siti Salwa, Shamsi, Suhaili, Hassan, Masriana, Marikkar, Nazrim. (1403). 'Exploring the Therapeutic Potential of Malaysian Stevia rebaudiana (Bertoni) Bertoni leaves essential oil: A Comprehensive Study on Cell Viability and Biological Effects for Topical Applications', سامانه مدیریت نشریات علمی, 13(3), pp. 504-512. doi: 10.22034/jmpb.2024.361730.1541
Mohamad Zen, Nur Izzati, Zaidan, Uswatun Hasanah, Abd. Gani, Siti Salwa, Shamsi, Suhaili, Hassan, Masriana, Marikkar, Nazrim. Exploring the Therapeutic Potential of Malaysian Stevia rebaudiana (Bertoni) Bertoni leaves essential oil: A Comprehensive Study on Cell Viability and Biological Effects for Topical Applications. سامانه مدیریت نشریات علمی, 1403; 13(3): 504-512. doi: 10.22034/jmpb.2024.361730.1541

Exploring the Therapeutic Potential of Malaysian Stevia rebaudiana (Bertoni) Bertoni leaves essential oil: A Comprehensive Study on Cell Viability and Biological Effects for Topical Applications

Journal of Medicinal plants and By-products
مقاله 2، دوره 13، شماره 3، آذر 2024، صفحه 504-512    اصل مقاله (1.01 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2024.361730.1541
نویسندگان
Nur Izzati Mohamad Zen1؛ Uswatun Hasanah Zaidan* 1، 2؛ Siti Salwa Abd. Gani1، 3؛ Suhaili Shamsi2؛ Masriana Hassan4؛ Nazrim Marikkar5
1Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
3Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
4Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
5National Institute of Fundamental Studies Hanthana Road, 20000 Kandy, Sri Lanka
چکیده
The abundance of plant resources in Malaysia has gained the attention of many researchers to explore more pharmacological effects of plant’s essential oil (EO). EO from Stevia rebaudiana (Bertoni) Bertoni (SrB-EO) leaves consists of a rich blend of a variety of volatile molecules and bioactive compounds which currently attract the attention of a lot of researchers due to its high potential to play a role in the therapeutic activity. In the present study, SrB-EO used was extracted using a Soxhlet extractor in absolute ethanol. The cell viability on SrB-EO has been investigated using Hs27 skin fibroblast cells against two biological assessments; anti-inflammatory and antimicrobial assays. It shows that 500 µg/mL was the highest concentration of SrB-EO which is considered non-toxic (82.08% cell viability) and demonstrates a primary significant cell proliferative effect toward cells. The maximum inhibition percentage of nitrite of SrB-EO was 33.12%, which showed a good anti-inflammatory property and SrB-EO showed an antimicrobial effect toward Gram-positive bacteria; Bacillus subtilis B29 and Staphylococcus aureus ATCC 43300. The minimal inhibition concentration (MIC) of B. subtilis and S. aureus were 6.25 mg/mL and 3.13 mg/mL of SrB-EO respectively, and the minimal bactericidal concentration (MBC) of B. subtilis and S. aureus were 12.50 mg/mL and 10.42 mg/mL of SrB-EO, respectively. This study concluded that a low concentration of SrB-EO is non-toxic toward human skin cells and these EOs possess satisfactory anti-inflammatory and antibacterial properties. Thus, SrB-EO can be used as an alternative therapeutic agent for topical application in the medicinal field.
کلیدواژه‌ها
Essential oil؛ Stevia rebaudiana Bertoni؛ Cytotoxicity؛ Anti-inflammatory؛ Antimicrobial activity
مراجع
  1. Ambrose D.C.P., Manickavasagan A, Naik R. Leafy Medicinal Herbs: Botany, Chemistry, Postharvest Technology and Uses. CABI; 2016:xiii + 282 pp.
  2. Orellana-Paucar A.M. Steviol Glycosides from Stevia rebaudiana: An Updated Overview of Their Sweetening Activity, Pharmacological Properties, and Safety Aspects. Molecules. 2023;28(3):1258.
  3. Baser K.H.C., Buchbauer G. Handbook of Essential Oils: Science, Technology, and Applications. CRC Press; 2020.
  4. Padalia R.C., Verma D.K., Arora C., Mahish P.K. Essential Oils: Sources, Production and Applications. Walter de Gruyter GmbH & Co KG; 2023.
  5. Aggarwal S., Agarwal S., Jalhan S. Essential oils as novel human skin penetration enhancer for transdermal drug delivery: A review. Int. J. Pharm. Biol. Sci. 2013;4(1):857-868.
  6. Costa M.F., Durço A.O., Rabelo T.K., Barreto R. de S.S., Guimarães A.G. Effects of Carvacrol, Thymol and essential oils containing such monoterpenes on wound healing: a systematic review. J. Pharm. Pharmacol. 2019;71(2):141-155.
  7. Arooj B., Asghar S., Saleem M., Khalid S.H., Asif M., Chohan T., Khan I.U., Zubair H.M., Yaseen H.S. Anti-inflammatory mechanisms of eucalyptol rich Eucalyptus globulus essential oil alone and in combination with flurbiprofen. Inflammopharmacology. 2023;31(4):1849-1862.
  8. Koyama S., Purk A., Kaur M., Soini H.A., Novotny M.V., Davis K., Kao C.C., Matsunami H., Mescher A. Beta-caryophyllene enhances wound healing through multiple routes. PloS One. 2019;14(12):e0216104.
  9. Mohamad Zen N.I., Zaidan U.H., Abd Gani S.S., Shamsi S., Halmi M.I.E. Essential Oil Constituents Assessment and Antioxidant Profiles of Stevia rebaudiana Bertoni Leaves from Malaysia. Malays. Appl. Biol. 2019;48:55-62.
  10. Zaidan U.H., Hamid S.N.M., Mat Yusof M.F., Ahmad S., Shamsi S. Chemical Evaluation And Antioxidant Properties of Extracts and Essential Oil from Stevia rebaudiana Leaves. Malays Appl Biol. 2018;47(2):15-23.
  11. Ghiulai R., Roşca O.J., Antal D.S., Mioc M., Mioc A., Racoviceanu R., Macaşoi I., Olariu T., Dehelean C., Creţu O.M., Voicu M., Şoica C. Tetracyclic and Pentacyclic Triterpenes with High Therapeutic Efficiency in Wound Healing Approaches. Molecules. 2020;25(23):5557. doi:10.3390/molecules25235557
  12. Bensouilah J., Buck P. Aromadermatology: Aromatherapy in the Treatment and Care of Common Skin Conditions. Routledge; 2021.
  13. Aslantürk Ö.S. In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. In: Genotoxicity - A Predictable Risk to Our Actual World. InTech; 2018. doi:10.5772/intechopen.71923
  14. Muanda F.N., Soulimani R., Diop B., Dicko A. Study on chemical composition and biological activities of essential oil and extracts from Stevia rebaudiana Bertoni leaves. LWT - Food Sci. Technol. 2011;44(9):1865-1872. doi:10.1016/j.lwt.2010.12.002
  15. Ericsson H.M., Sherris J.C. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol. Microbiol. Scand. [B] Microbiol. Immunol. 1971;217:Suppl 217:1+-Suppl 217:1+.
  16. Li Z.H., Cai M., Liu Y.S., Sun P.L., Luo S.L. Antibacterial Activity and Mechanisms of Essential Oil from Citrus medica L. Var. Sarcodactylis. Molecules. 2019;24(8).
  17. Chan L.L.Y., Rice W.L., Qiu J. Observation and quantification of the morphological effect of trypan blue rupturing dead or dying cells. PLoS One. 2020;15(1).
  18. Babakhanova G., Zimmerman S.M., Pierce L.T., Sarkar S., Schaub N.J., Simon C.G.Jr. Quantitative, traceable determination of cell viability using absorbance microscopy. PLoS One. 2022;17(1):e0262119.
  19. Kamiloglu S., Sari G., Ozdal T., Capanoglu E. Guidelines for cell viability assays. Food Front. 2020;1(3):332-349.
  20. Demetzos C., Pippa N. Thermodynamics and Biophysics of Biomedical Nanosystems: Applications and Practical Considerations. Springer; 2019.
  21. International Standard I. ISO 10993-5:2009 - Biological evaluation of medical devices- Part 5: Tests for in vitro cytotoxicity. Published online 2009.
  22. Toiu A., Mocan A., Vlase L., Pârvu A.E., Vodnar D.C., Gheldiu A.M., Moldovan C., Oniga I. Phytochemical Composition, Antioxidant, Antimicrobial and in vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. ("Nobleman's Beard" - Barba Împăratului). Front. Pharmacol. 2018;9(MAR):7-7.
  23. Malik S., ed. Essential Oil Research: Trends in Biosynthesis, Analytics, Industrial Applications and Biotechnological Production. Springer, 2019.
  24. Benevides Bahiense J., Marques F.M., Figueira M.M., Vargas T.S., Kondratyuk T.P., Endringer D.C., Scherer R., Fronza M. Potential anti-inflammatory, antioxidant and antimicrobial activities of Sambucus australis. Pharm. Biol. 2017;55(1):991-997.
  25. Pinto R., Antunes F., Pires J., Silva-Herdade A., Pinto M.L. A Comparison of Different Approaches to Quantify Nitric Oxide Release from NO-Releasing Materials in Relevant Biological Media. Molecules. 2020;25(11):2580. doi:10.3390/molecules25112580
  26. Domb A.J., Kunduru K.R., Farah S. Antimicrobial Materials for Biomedical Applications. Royal Society of Chemistry; 2019.
  27. Sateriale D., Forgione G., De Cristofaro G.A., Facchiano S., Boscaino F., Pagliuca C., Colicchio R., Salvatore P., Paolucci M., Pagliarulo C. Towards Green Strategies of Food Security: Antibacterial Synergy of Essential Oils from Thymus vulgaris and Syzygium aromaticum to Inhibit Escherichia coli and Staphylococcus aureus Pathogenic Food Isolates. Microorganisms. 2022;10(12):2446.
  28. Zaidan U. H., Mohamad Zen N.I., Amran N.A., Shamsi S., Abd Gani S.S. Biochemical evaluation of phenolic compounds and steviol glycoside from Stevia rebaudiana extracts associated with in vitro antidiabetic potential. Biocatal. Agric. Biotechnol. 2019;18:101049-101049.
  29. Liu K., Zhang X., Xie L., Deng M., Chen H., Song J., Long J., Li X., Luo J. Lupeol and its derivatives as anticancer and anti-inflammatory agents: Molecular mechanisms and therapeutic efficacy. Pharmacol. Res. 2021;164:10537.
  30. Pawar S., Bhane P., Jha U. Review on Betel Pepper Leaf for Anti Inflammatory Activity. World J. Pharm. Res. 2021;11(2):1196-1208.
  31. Barboza J.N., da Silva Maia Bezerra Filho C., Silva R.O., Medeiros J.V.R., de Sousa D.P. An Overview on the Anti-inflammatory Potential and Antioxidant Profile of Eugenol. Oxid. Med. Cell. Longev. 2018;2018:3957262.
  32. Miguel M.G. Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules. 2010;15(12):9252-9287.
  33. Lis-Balchin M. Aromatherapy Science: A Guide for Healthcare Professionals. 1st ed. Pharmaceutical Press; 2006:446. doi:10.1111/j.2042-7166.2006.tb04705.x
  34. Valerio F., Mezzapesa G.N., Ghannouchi A., Mondelli D., Logrieco A.F., Perrino E.V. Characterization and Antimicrobial Properties of Essential Oils from Four Wild Taxa of Lamiaceae Family Growing in Apulia. Agronomy. 2021;11(7):1431.
  35. Tran V.T., Nguyen T.B., Nguyen H.C., Do N.H.N., Le P.K. Recent applications of natural bioactive compounds from Piper betle (L.) leaves in food preservation. Food Control. 2023;154:110026.
  36. Andrade-Ochoa S., Chacón-Vargas K.F., Sánchez-Torres L.E., Rivera-Chavira B.E., Nogueda-Torres B., Nevárez-Moorillón G.V. Differential Antimicrobial Effect of Essential Oils and Their Main Components: Insights Based on the Cell Membrane and External Structure. Membranes. 2021;11(6):405. doi:10.3390/membranes11060405
  37. Byrd A.L., Belkaid Y., Segre J.A. The human skin microbiome. Nat. Rev. Microbiol. 2018;16(3):143-155.
  38. Hernandez-Valdes J.A., Zhou L., de Vries M.P., Kuipers O.P. Impact of spatial proximity on territoriality among human skin bacteria. NPJ Biofilms Microbiomes. 2020;6(1):1-13.
  39. Patterson J.E., McElmeel L., Wiederhold N. P. In Vitro Activity of Essential Oils Against Gram-Positive and Gram-Negative Clinical Isolates, Including Carbapenem-Resistant Enterobacteriaceae. Open Forum Infect Dis. 2019;6(12): ofz502.
آمار
تعداد مشاهده مقاله: 605
تعداد دریافت فایل اصل مقاله: 524


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب