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Saeedi, Negin, Vatani, Mahnaz, Nejat, Seyed Hassan, Omidi, Nasrin, Moosavi, Mona, Soltani Borchaloee, Arash. (1403). Multiplex PCR amplification for the detection of Biofilm and extended-spectrum Beta-Lactamase resistance genes and antibiotic resistance patterns in uropathogenic E. coli. سامانه مدیریت نشریات علمی, 80(1), 125-130. doi: 10.32592/ARI.2025.80.1.125
Negin Saeedi; Mahnaz Vatani; Seyed Hassan Nejat; Nasrin Omidi; Mona Moosavi; Arash Soltani Borchaloee. "Multiplex PCR amplification for the detection of Biofilm and extended-spectrum Beta-Lactamase resistance genes and antibiotic resistance patterns in uropathogenic E. coli". سامانه مدیریت نشریات علمی, 80, 1, 1403, 125-130. doi: 10.32592/ARI.2025.80.1.125
Saeedi, Negin, Vatani, Mahnaz, Nejat, Seyed Hassan, Omidi, Nasrin, Moosavi, Mona, Soltani Borchaloee, Arash. (1403). 'Multiplex PCR amplification for the detection of Biofilm and extended-spectrum Beta-Lactamase resistance genes and antibiotic resistance patterns in uropathogenic E. coli', سامانه مدیریت نشریات علمی, 80(1), pp. 125-130. doi: 10.32592/ARI.2025.80.1.125
Saeedi, Negin, Vatani, Mahnaz, Nejat, Seyed Hassan, Omidi, Nasrin, Moosavi, Mona, Soltani Borchaloee, Arash. Multiplex PCR amplification for the detection of Biofilm and extended-spectrum Beta-Lactamase resistance genes and antibiotic resistance patterns in uropathogenic E. coli. سامانه مدیریت نشریات علمی, 1403; 80(1): 125-130. doi: 10.32592/ARI.2025.80.1.125

Multiplex PCR amplification for the detection of Biofilm and extended-spectrum Beta-Lactamase resistance genes and antibiotic resistance patterns in uropathogenic E. coli

Archives of Razi Institute
مقاله 14، دوره 80، شماره 1، فروردین و اردیبهشت 2025، صفحه 125-130    اصل مقاله (364.6 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.1.125
نویسندگان
Negin Saeedi1؛ Mahnaz Vatani2؛ Seyed Hassan Nejat3؛ Nasrin Omidi4؛ Mona Moosavi5؛ Arash Soltani Borchaloee* 6
1Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
2Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
4Department of Biology, Miyaneh Branch,Islamic Azad University, Miyaneh, Iran
5Department of physiology, Faculty of animal Sciences, Tarbiat modares University,Tehran, Iran
6SabaBiomedicals Science-Based Company, Tehran, Iran
چکیده
The issue of urinary tract infections, particularly those caused by Escherichia coli belonging to the Enterobacteriaceae family, has received considerable critical attention and is evaluated as the second most common infection in humans. Uropathogenic Escherichia coli (UPEC), which is virulent, produces extended-spectrum beta-lactamase (ESBL), and is also multidrug-resistant (MDR), is considered to be a common growing public health issue worldwide. This could be a contributing factor for UTIs to become far more severe, first-line antibiotics to become less effective, and thus the rates of morbidity and mortality to rise. In this study, 73 Escherichia coli strains have been isolated from urine specimens. The antibiotic susceptibility of the isolates was evaluated through the disc agar diffusion method. Taken together, the resistance pattern is the underlying basis of MDR. Three significant biofilm genes and antimicrobial resistance mechanisms were evaluated in these isolates with ten typical antibiotic discs, considering that the data was processed using SPSS statistical software, version 25. In this study, 73 isolates of E. coli were examined, in which pap gene is present in 89% of isolates, fimH gene is present in 86.3% of isolates, and sfa gene is present in 69.9% of isolates. Furthermore, the beta-lactamase gene blaTEM, blaCTX-M, and blaSHV gene frequency were 90.4%, 79.5, and 50.7% respectively. The results of the antibiotic resistance pattern elucidated that most of the isolates were resistant to Ampicillin, Imipenem, and Amoxicillin respectively. This study suggests that the alarming level of virulent ESBL-producer E. coli strains used in this experiment necessitates the performing of an antibiotic stewardship program, regional screening of ESBL-producers and their virulence properties to select appropriate antibiotics, or designing new therapeutic methods for UTIs by inactivation of the essential virulence factors of UPECs.
کلیدواژه‌ها
E. coli؛ UPEC؛ ESBL؛ UTIs؛ Biofilm
مراجع
  1. Munkhdelger Y, Gunregjav N, Dorjpurev A, Juniichiro N, Sarantuya J. Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia. The Journal of Infection in Developing Countries. 2017;11; 51-57.
  2. Sarowska J, Futoma-Koloch B, Jama-Kmiecik A, Frej-Madrzak M, Ksiazczyk M, Bugla-Ploskonska G, Choroszy-Krol I. Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports. Gut pathogens. 2019;11;1-6.
  3. Borchaloee, A. S. Investigating Differences between Virulence Factors of Escherichia coli and Pseudomonas aeruginosa in Causing Urinary Tract Infections. I J Infectious Disea; 2023; 4; 1-8.
  4. Baby S, Karnaker VK, Geetha R. Adhesins of uropathogenic Escherichia coli (UPEC). Int J Med Microbiol Trop Dis. 2016;1;10–18.
  5. Dadi BR, Abebe T, Zhang L, Mihret A, Abebe W, Amogne W. Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia. BMC infectious diseases. 2020;20;1-2.
  6. Frömmel U, Lehmann W, Rödiger S, Böhm A, Nitschke J, Weinreich J, Groß J, Roggenbuck D, Zinke O, Ansorge H, Vogel S. Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins. Applied and environmental microbiology. 2013;79;5814-5829.
  7. Sadiq FA, Burmølle M, Heyndrickx M, Flint S, Lu W, Chen W, Zhao J, Zhang H. Community-wide changes reflecting bacterial interspecific interactions in multispecies biofilms. Crit Rev Microbiol. 2021;47:338–58.
  8. Naziri Z, Kilegolan JA, Moezzi MS, Derakhshandeh A. Biofilm formation by uropathogenic Escherichia coli: a complicating factor for treatment and recurrence of urinary tract infections. Journal of Hospital Infection. 2021;117;9-16.
  9. Usein CR, Damian M, Tatu-Chitoiu D, Capusa C, Fagaras R, Tudorache D, Nica M, Le Bouguénec C. Prevalence of virulence genes in Escherichia coli strains isolated from Romanian adult urinary tract infection cases. J Cell Mol Med. 2001; 5;303-310.
  10. Schwartz DJ, Conover MS, Hannan TJ, Hultgren SJ. Uropathogenic Escherichia coli superinfection enhances the severity of mouse bladder infection. PLoS Pathog. 2015; 11;e1004599
  11. Abe CM, Salvador FA, Falsetti IN, Vieira MA, Blanco J, Blanco JE, Blanco M, Machado AM, Elias WP, Hernandes RT, Gomes TA. Uropathogenic Escherichia coli (UPEC) strains may carry virulence properties of diarrhoeagenic coli. FEMS Immunol Med Microbiol. 2008; 52;397-406.
  12. Nys S, Van Merode T, Bartelds AI, Stobberingh EE. Antibiotic treatment and resistance of unselected uropathogens in the elderly. International journal of antimicrobial agents. 2006;27;236-241.
  13. Pitout JD, Nordmann P, Laupland KB, Poirel L. Emergence of Enterobacteriaceae producing extended-spectrum β-lactamases (ESBLs) in the community. Journal of antimicrobial chemotherapy. 2005;56;52-9.
  14. Demirel I, Kinnunen A, Önnberg A, Söderquist B, Persson K. Comparison of host response mechanisms evoked by extended spectrum beta lactamase (ESBL)-and non-ESBL-producing uropathogenic coli. BMC microbiology. 2013;13;1-9.
  15. Yamamoto S, Terai A, Yuri K, Kurazono H, Takeda Y, Yoshida O: Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol Med Microbiol 1995, 12;85–90.
  16. Struve C, Krogfelt KA: In vivo detection of Escherichia coli type 1 fimbrial expression and phase variation during experimental urinary tract infection. Microbiol 1999; 145; 2683–2690
  17. Le Bouguenec C, Archambaud M, Labigne A: Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J Clin Microbiol 1992; 30; 1189–1193.
  18. Shah HA, Syed A, Bhat MA, Kakru DK, Farooq S, Qureshi S, et al. Genomic Determinants and Antimicrobial Resistance Pattern of Clinical Isolates of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli. J Pure Appl Microbiol. 2023;17(3):1679-1690.
  19. Fang H, Lundberg C, Olsson-Liljequist B, et al. Molecular epidemiological analysis of Escherichia coli isolates producing extended-spectrum b-lactamases for identification of nosocomial outbreaks in Stockholm, Sweden. J Clin Microbiol. 2004;42;5917-5920
  20. Monstein HJ, Ostholm-Balkhed Aֵ, Nilsson MV, Nilsson M, Dornbusch K, Nilsson LE. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. Apmis. 2007;115;1400-1408.
  21. Rahdar M, Rashki A, Miri HR, Ghalehnoo MR. Detection of pap, sfa, afa, foc, and fim adhesin-encoding operons in uropathogenic Escherichia coli isolates collected from patients with urinary tract infection. Jundishapur J Microbiol. 2015; 8; e22647.
  22. Yazdi M, Bouzari M, Ghaemi EA. Detection of fim, pap, sfa and afa adhesin-encoding operons in Escherichia coli strains isolated from urinary tract infections. Medical Laboratory Journal. 2018;12;10-15.
  23. Saki A, Mirzaei M. Frequency of fimberial virulence genes (fim, pap, sfa) in Escherichia coli isolated from the patients with urinary tract infections from selective hospitals of Tehran, Boroujerd and sanandej citu in 2015-2016. Journal of Shahid Sadoughi University of Medical Sciences. 2017;24; 913-923.
  24. Naziri Z, Derakhshandeh A, Borchaloee AS, Poormaleknia M, Azimzadeh N. Treatment failure in urinary tract infections: a warning witness for virulent multi-drug resistant ESBL-producing Escherichia coli. Infection and drug resistance. 2020;1839-1850.
  25. Habeeb MA, Sarwar Y, Ali A, Salman M, Haque A. Rapid emergence of ESBL producers in E. coli causing urinary and wound infections in Pakistan. Pak J Med Sci. 2013;29; 540-544.
  26. Tiba MR, Yano T, Leite DDS. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Rev Inst Med Trop. 2008; 50 ;255-260.
  27. Arisoy M, Ayser D, Ekim M, Ozel D, Kose S K, Ozsoy E D, et al. Detection of virulence factors of coli from children by Duplex PCR. Int J Clin Pract. 2005; 60; 170-73.
  28. Cristea VC, Gheorghe I, Barbu IC, et al. Snapshot of phylogenetic groups, virulence, and resistance markers in Escherichia coli uropathogenic strains isolated from outpatients with urinary tract infections in Bucharest, Romania. BioMed Res Int. 2019;5712371.
  29. Sadeghi M, Sedigh Ebrahim-Saraie H, Mojtahedi A. Prevalence of ESBL and AmpC genes in E. coli isolates from urinary tract infections in the north of Iran. New Microbes New Infect. 2021;45; 100947.
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