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Mahjoub, Mohammad Ali, Iman, Maryam, salimisabour, ebrahim, anbara, hojat. (1403). Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies. سامانه مدیریت نشریات علمی, 79(6), 1271-1280. doi: 10.32592/ARI.2024.79.6.1271
Mohammad Ali Mahjoub; Maryam Iman; ebrahim salimisabour; hojat anbara. "Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies". سامانه مدیریت نشریات علمی, 79, 6, 1403, 1271-1280. doi: 10.32592/ARI.2024.79.6.1271
Mahjoub, Mohammad Ali, Iman, Maryam, salimisabour, ebrahim, anbara, hojat. (1403). 'Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies', سامانه مدیریت نشریات علمی, 79(6), pp. 1271-1280. doi: 10.32592/ARI.2024.79.6.1271
Mahjoub, Mohammad Ali, Iman, Maryam, salimisabour, ebrahim, anbara, hojat. Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies. سامانه مدیریت نشریات علمی, 1403; 79(6): 1271-1280. doi: 10.32592/ARI.2024.79.6.1271

Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies

Archives of Razi Institute
دوره 79، شماره 6، بهمن و اسفند 2024، صفحه 1271-1280    اصل مقاله (748.11 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.6.1271
نویسندگان
Mohammad Ali Mahjoub1؛ Maryam Iman* 1؛ ebrahim salimisabour2؛ hojat anbara3
1Department of Pharmaceutics, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Department of Pharmacognosy, Faculty of Pharmacy, Baqiatallah University of Medical Sciences, Tehran, Iran.
3Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
چکیده
This study assessed the anti-inflammatory effects of chitosan (CHT) film loaded with Arnebia euchroma extract, both in vitro and in vivo. Arnebia euchroma contains shikonin (SHKN), a naphthoquinone with significant anti-inflammatory, antimicrobial, and wound-healing properties. High-quality SHKN extract was standardized and incorporated into CHT films, which were then evaluated for stability, drug release, antibacterial effectiveness, and anti-inflammatory activity.
Two concentrations of SHKN were used in CHT film preparations. In vitro studies showed that the optimal CHT film formulation was stable over four weeks at 4°C. A biphasic SHKN release profile from the films was observed, indicative of a sustained drug release mechanism. The films exhibited a strong antibacterial effect against Staphylococcus aureus (S. aureus) due to the presence of SHKN, but no such effect against Escherichia coli (E. coli). A synergistic antibacterial effect was noted when CHT was combined with A. euchroma extract against S. aureus.
In vivo, CHT film with A. euchroma extract showed anti-inflammatory effects in a mice paw swelling test, comparable to betamethasone (mice were divided into 4 groups of 6) the difference was not statistically significant (p value > 0.05). Histological examination confirmed the reduction of immune cell infiltration in the treatment group. The study concluded that CHT films containing A. euchroma extract exhibit promising anti-inflammatory and antimicrobial properties. They are also suitable for use as wound dressings, having high portability, mechanical strength, and being non-adhesive, making them readily applicable for medical and pharmaceutical use, as well as potential carriers for antimicrobial agents and antioxidants in various industries. In conclusion the use of Chitosan films embedded with Arnebia euchroma extract could offer an accessible and convenient therapeutic application for various wounds and inflammatory conditions.
کلیدواژه‌ها
Arnebia euchroma؛ Chitosan؛ Shikonin؛ Anti-inflammatory؛ Anti-bacterial
عنوان مقاله [English]
بررسی اثرات ضد التهابی فیلم کیتوزانی دارای عصاره ابوخلسا به صورت برون تن و درون تن
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