اخبار و اعلانات

 آمار

تعداد نشریات287
تعداد نشریات سازمان85
تعداد شماره‌ها3,159
تعداد مقالات35,108
تعداد مشاهده مقاله50,533,303
تعداد دریافت فایل اصل مقاله90,481,511
S L, Varsha, Shettar, Arun, Hoskeri, Joy, A B, Vedamurthy. (1403). In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia. سامانه مدیریت نشریات علمی, 79(5), 1023-1030. doi: 10.32592/ARI.2024.79.5.1023
Varsha S L; Arun K Shettar; Joy H Hoskeri; Vedamurthy A B. "In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia". سامانه مدیریت نشریات علمی, 79, 5, 1403, 1023-1030. doi: 10.32592/ARI.2024.79.5.1023
S L, Varsha, Shettar, Arun, Hoskeri, Joy, A B, Vedamurthy. (1403). 'In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia', سامانه مدیریت نشریات علمی, 79(5), pp. 1023-1030. doi: 10.32592/ARI.2024.79.5.1023
S L, Varsha, Shettar, Arun, Hoskeri, Joy, A B, Vedamurthy. In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia. سامانه مدیریت نشریات علمی, 1403; 79(5): 1023-1030. doi: 10.32592/ARI.2024.79.5.1023

In-vitro Pharmacological activity of Endophyte Aspergillus austwickii isolated from leaves of Premna serratifolia

Archives of Razi Institute
مقاله 19، دوره 79، شماره 5، آذر و دی 2024، صفحه 1023-1030    اصل مقاله (527.4 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.5.1023
نویسندگان
Varsha S L1؛ Arun K Shettar2؛ Joy H Hoskeri3؛ Vedamurthy A B* 4
1P G Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad 580003, Karnataka, India.
2Division of Pre-clinical Research and Drug Development, Cytxon Biosolutions Pvt Ltd, Hubli-580031, Karnataka, India.
3Department of Bioinformatics and Biotechnology, Karnataka State Akkamahadevi Women’s University, Vijayapura, Karnataka, India.
4PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad-580003
چکیده
Diabetes mellitus is one of the most widely occurring non communicable diseases spreading rapidly worldwide that occurs as a result of non-regulation of glucose in the blood stream or because of improper functioning of the enzymes α- amylase and α-glucosidase. Endophytes are the microorganisms that reside symbiotically inside the living tissues of plants. Endophytic fungi have the ability to synthesize various important bioactive metabolites. The current study was aimed to explore the less reported endophytic fungus Aspergillus austwickii isolated from ethnobotanical medicinal plant Premna serratifolia L. The methanolic extract of the endophytic fungus was subjected to activities like in vitro antioxidant assays, anti-inflammatory assay, antidiabetic assay in addition to exploring their total phenolic and total flavonoid content along with the phytochemicals present. The results revealed that Aspergillus austwickii exhibited phytoconstituents like alkaloids, phenols, flavonoids, tannins and carbohydrates. The total phenolic content and total flavonoid content of the fungus was found to be 22.048 µg GAE/g and 18.828 µg GAE/g. The crude extract showed 46.20+0.53% of antioxidant activity with an IC50 value of 128.69 µg/mL for radical scavenging by DPPH. It also exhibited 71.86+0.34% of anti-inflammatory activity by protein denaturation assay. Notably, it revealed, antidiabetic activity for both alpha amylase and alpha glucosidase with the percentage of inhibition of 68.22+0.17% and 73.72+0.18% with an IC50 value of 178.10 µg/mL and 166.16µg/mL respectively. The current study shows that the methanolic extract of Aspergillus austwickii possesses considerable antioxidant, anti-inflammatory and antidiabetic capabilities. The findings of the current research can be explored for the future research to find out new set of natural drugs which can be used against several disorders.
کلیدواژه‌ها
Antioxidant؛ alpha(α) amylase؛ alpha(α) glucosidase؛ anti-inflammatory activity
سایر فایل های مرتبط با مقاله
مراجع
  1. Narkhede MB, Ajimire PV, Wagh AE, Mohan M, Shivashanmugam AT. In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. Asian Journal of Plant Science and Research. 2011;1(2):101-6.
  2. L’heveder R, Nolan T. International diabetes federation. Diabetes research and clinical practice. 2013;101(3):349-51.
  3. Alqahtani AS, Hidayathulla S, Rehman MT, ElGamal AA, Al-Massarani S, Razmovski-Naumovski V, et al. Alpha-amylase and alpha-glucosidase enzyme inhibition and antioxidant potential of 3-oxolupenal and katononic acid isolated from Nuxia oppositifolia. Biomolecules. 2019;10(1):61.
  4. Strobel G, Daisy B, Castillo U. The biological promise of microbial endophytes and their natural products. Plant Pathol J. 2005;4(2):161-76.
  5. Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. Journal of ethnopharmacology. 2002;81(1):81-100.
  6. Deshmukh SK, Dufossé L, Chhipa H, Saxena S, Mahajan GB, Gupta MK. Fungal endophytes: A potential source of antibacterial compounds. Journal of Fungi. 2022;8(2):164.
  7. Lacerda ÍC, Polonio JC, Golias HC. Endophytic fungi as a source of antiviral compounds–a review. Chemistry & Biodiversity. 2022;19(6):e202100971.
  8. Toghueo RM, Boyom FF. Endophytes from ethno-pharmacological plants: Sources of novel antioxidants-A systematic review. Biocatalysis and Agricultural Biotechnology. 2019;22:101430.
  9. Agrawal S, Samanta S, Deshmukh SK. The antidiabetic potential of endophytic fungi: Future prospects as therapeutic agents. Biotechnology and Applied Biochemistry. 2022;69(3):1159-65.
  10. Kumaresan V, Suryanarayanan TS. Occurrence and distribution of endophytic fungi in a mangrove community. Mycological Research. 2001;105(11):1388-91.
  11. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution. 2018;35(6):1547.
  12. Devi NN, Prabakaran JJ, Wahab F. Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica. Asian Pacific Journal of Tropical Biomedicine. 2012;2(3):S1280-4.
  13. Yadav M, Yadav A, Yadav JP. In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam. Asian Pacific journal of tropical medicine. 2014;7:S256-61.
  14. Ordonez AA, Gomez JD, Vattuone MA. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food chemistry. 2006;97(3):452-8.
  15. Chang LW, Yen WJ, Huang SC, Duh PD. Antioxidant activity of sesame coat. Food chemistry. 2002;78(3):347-54.
  16. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999;269(2):337-41.
  17. Govindappa M, Channabasava R, Sowmya DV, Meenakshi J, Shreevidya MR, Lavanya A, Santoyo G, Sadananda TS. Phytochemical screening, antimicrobial and in vitro anti-inflammatory activity of endophytic extracts from Loranthus sp. Pharmacognosy Journal. 2011;3(25):82-90.
  18. Shoba S, Mythili S. Biological activities of endophytic fungus Cochliobolus sp. AL24 isolated from Aerva lanata L. L. Indian J Pharm Educ. 2018;52:277-83.
  19. Singh B, Kaur A. Antidiabetic potential of a peptide isolated from an endophytic Aspergillus awamori. Journal of Applied Microbiology. 2016;120(2):301-11.
  20. Bisht R, Sharma D, Agrawal PK. Antagonistic and antibacterial activity of endophytic fungi isolated from needle of Cupressus torulosa D. Don. Asian J Pharm Clin Res. 2016;9(3):282-8.
  21. Alurappa R, Chowdappa S. Antimicrobial Activity and Phytochemical Analysis of Endophytic Fungal Extracts Isolated from Ethno-Pharmaceutical Plant Rauwolfia tetraphylla L. Journal of Pure & Applied Microbiology. 2018;12(1):317-332.
  22. Lai HY, Lim YY, Kim KH. Blechnum orientale Linn-a fern with potential as antioxidant, anticancer and antibacterial agent. BMC Complementary and Alternative Medicine. 2010;10:1-8.
  23. Khalil D, El-Zayat SA, El-Sayed MA. Phytochemical screening and antioxidant potential of endophytic fungi isolated from Hibiscus sabdariffa. Journal of Applied Biotechnology Reports. 2020;7(2):116-24.
  24. Jayant KK, Vijayakumar BS. In-Vitro anti-oxidant and anti-diabetic potential of endophytic fungi associated with Ficus religiosa. Italian Journal of Mycology. 2021;50:10-20.
  25. Moharram AM, Zohri AA, Omar HM, Abd El-Ghani OA. In vitro assessment of antimicrobial and anti-inflammatory potential of endophytic fungal metabolites extracts. European Journal of Biological Research. 2017;7(3):234-44.
  26. Satari AH, Zargar MI, Shah WA, Bansal R, Bhat MF. Isolation, molecular identification, phytochemical screening and in vitro anti-oxidant activity of endophytic fungi from Achilea millefolium Linn. Journal of Pharmacognosy and Phytochemistry. 2018;7(4):87-92.
  27. Sharaf MH, Abdelaziz AM, Kalaba MH Radwan AA, Hashem AH. Antimicrobial, antioxidant, cytotoxic activities and phytochemical analysis of fungal endophytes isolated from Ocimum basilicum. 2022;1-19.
  28. Fawole OA, Amoo SO, Ndhala AR, Light ME, Finnie JF, Van Staden J. Anti-inflammatory, anticholinestrase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa. Journal of Ethnopharmacology. 2010;127(2):235-241.
آمار
تعداد مشاهده مقاله: 1,133
تعداد دریافت فایل اصل مقاله: 500


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب