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Karimian, Paridokht, Mojtahedi, Ali, Anvari, Masoumeh, Mesbahi, G, Ghalandari, Maryam. (1403). The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran. سامانه مدیریت نشریات علمی, 79(5), 955-961. doi: 10.32592/ARI.2024.79.5.955
Paridokht Karimian; Ali Mojtahedi; Masoumeh Anvari; G Mesbahi; Maryam Ghalandari. "The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran". سامانه مدیریت نشریات علمی, 79, 5, 1403, 955-961. doi: 10.32592/ARI.2024.79.5.955
Karimian, Paridokht, Mojtahedi, Ali, Anvari, Masoumeh, Mesbahi, G, Ghalandari, Maryam. (1403). 'The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran', سامانه مدیریت نشریات علمی, 79(5), pp. 955-961. doi: 10.32592/ARI.2024.79.5.955
Karimian, Paridokht, Mojtahedi, Ali, Anvari, Masoumeh, Mesbahi, G, Ghalandari, Maryam. The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran. سامانه مدیریت نشریات علمی, 1403; 79(5): 955-961. doi: 10.32592/ARI.2024.79.5.955

The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran

Archives of Razi Institute
مقاله 10، دوره 79، شماره 5، آذر و دی 2024، صفحه 955-961    اصل مقاله (332.32 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.5.955
نویسندگان
Paridokht Karimian1؛ Ali Mojtahedi* 2؛ Masoumeh Anvari3؛ G Mesbahi1؛ Maryam Ghalandari4
1Guilan University of Medical Sciences
2Iran University of Medical Sciences
3Islamic Azad University
4Shahid Sadoughi University of Medical Sciences and Health Services
چکیده
Staphylococcus aureus is the most important human pathogen, in community and hospital-acquired infections. The colonization rate of this organism is high in medical personnel and devices. Considering the importance of carriers in the transmission of S. aureus infection, this study investigated the origin of methicillin-resistant S. aureus (MRSA) isolated from Velayat and Poursina Hospitals of Rasht. In eight months, 500 samples were obtained from hospitalized patients, personnel, different surfaces, air, and devices in the intensive care unit (ICU) departments of Velayat and Poursina Hospitals. After the identification of MRSA strains by microbiological and biochemical standard methods, the DNA of the isolates was extracted. The spa typing of MRSA strains was done after determining the sequence of amplified protein A genes by polymerase chain reaction (PCR) using specific primers. Among 500 samples, 45 (9%) samples were infected with S. aureus and 31 (68.9%) MRSA strains were identified from different ICUs. For the first time, S. aureus was divided into three types with the help of the spa technique in the ICU of Rasht hospitals. Among the examined samples, the t14870 spa type prevailed (95.5%), which had been found in previous studies in different regions of Iran. However, two types, t8463 (2.2%) and t605 (2.2%), were obtained for the first time in Iran, which were MRSA and obtained from the noses of patients. High frequency of S. aureus isolates indicated in ICUs and personnel play a very effective role in the transmission of infection in the hospital, so need for infection control in the hospital is inevitable.
کلیدواژه‌ها
Methicillin resistant S. aureus (MRSA)؛ ICU؛ Staphylococcus protein A؛ spa typing
عنوان مقاله [English]
اولین گزارش از تیپ های spa t8463 و spa t605 در استافیلوکوکوس اورئوس مقاوم به متی سیلین جدا شده از بخش های مراقبت ویژه رشت، ایران
چکیده [English]
استافیلوکوکوس اورئوس مهم ترین پاتوژن انسانی در عفونت های اکتسابی از جامعه و بیمارستان است. میزان کلونیزاسیون این ارگانیسم در پرسنل پزشکی زیاد است. با توجه به اهمیت ناقلین در انتقال این عفونت، این مطالعه به بررسی منشا استافیلوکوکوس اورئوس مقاوم به متی سیلین (MRSA) جدا شده از بیمارستان های ولایت و پورسینای رشت پرداخت. در مدت هشت ماه، 500 نمونه از بیماران بستری، پرسنل، سطوح مختلف، هوا و دستگاه های بخش های آی سی یو بیمارستان های ولایت و پورسینا تهیه شد. پس از شناسایی سویه‌های MRSA با روش‌های استاندارد میکروبیولوژیکی و بیوشیمیایی، DNA جدایه‌ها استخراج شد. تیپ بندی spa سویه‌های MRSA پس از تعیین توالی ژن‌های پروتئین A تکثیر شده توسط PCR با استفاده از پرایمرهای اختصاصی انجام شد. از بین 500 نمونه، 45 (9%) نمونه آلوده به استافیلوکوکوس اورئوس و 31 (68.9%) سویه MRSA از واحدهای مراقبت‌های ویژه شناسایی شدند. استافیلوکوکوس اورئوس برای اولین بار با استفاده از تکنیک spa در بخش مراقبت های ویژه بیمارستان های رشت به سه تیپ تقسیم شد. در بین نمونه های مورد بررسی، تیپ spa t14870 (95.5 درصد) غالب بود که در مطالعات قبلی در مناطق مختلف ایران یافت شده بود. اما دو نوع t8463 (2.2%) و t605 (2.2%) برای اولین بار در ایران بدست آمد که MRSA بود و از بینی بیماران به دست آمد. فراوانی بالای ایزوله های استافیلوکوکوس اورئوس نشان داده شده در بخش های مراقبت ویژه و پرسنل نقش بسیار موثری در انتقال عفونت در بیمارستان دارد، بنابراین نیاز به کنترل عفونت در بیمارستان یک موضوع اجتناب ناپذیر است.
کلیدواژه‌ها [English]
استافیلوکوکوس اورئوس مقاوم به متی سیلین (MRSA), ICU, پروتئین A استافیلوکوکوس, تیپ بندی spa
مراجع
  1. Azizkhani M, Jafari F, Haghighi P, Dehghan M. Evaluating Contamination Level of Raw and Roasted Nuts Distributed in Commercial Markets in Mazandaran Province, Iran. Iranian Journal of Veterinary Medicine. 2020;14(2):167-176.
  2. Shariati A, Dadashi M, Moghadam MT, van Belkum A, Yaslianifard S, Darban-Sarokhalil D. Global prevalence and distribution of vancomycin resistant, vancomycin intermediate and heterogeneously vancomycin intermediate Staphylococcus aureus clinical isolates: a systematic review and meta-analysis. Scientific reports. 2020;10(1):12689.
  3. Gaddafi MS, Yakubu Y, Junaidu AU, Bello MB, Bitrus AA, Musawa AI, et al. Occurrence of Methicillin-resistant Staphylococcus aureus (MRSA) From Dairy Cows in Kebbi, Nigeria. Iranian Journal of Veterinary Medicine. 2023;17(1).
  4. Portela RP. New studies in the resistance to β-lactams and in cell wall damage survival in Staphylococcus aureus: an integrated approach. 2019.
  5. Ghanbari F, Nourmoradi H, Nazari A, Sadeghifard N, Pakzad I, Soroush S, et al. Molecular epidemiology and recycling of Staphylococcus aureus resistant to methicillin among the staff, patients, and surfaces in university Hospital in West Iran, Ilam. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders). 2023;23(1):42-51.
  6. Azizkhani M, Tooryan F. Methicillin resistant Staphylococcus aureus (MRSA) in pastry cream products sold in Amol (Iran). Iran J Vet Med. 2018;12(2).
  7. Yang X, Guo R, Zhang B, Xie B, Zhou S, Zhang B, Lai Q. Retrospective analysis of drug resistance characteristics and infection related risk factors of multidrug-resistant organisms (MDROs) isolated from the orthopedics department of a tertiary hospital. Scientific Reports. 2023;13(1):2199.
  8. Hashemizadeh Z, Dehkordi RS, Bazargani A, Javadi K, Hosseini-Nave H, Hadadi M. Evaluation of aminoglycoside modifying enzymes, SCCmec, coagulase gene and PCR-RFLP coagulase gene typing of Staphylococcus aureus isolates from hospitals in Shiraz, southwest of Iran. Heliyon. 2022;8(8):e10230.
  9. Kot B, Piechota M, Jakubczak A, Gryzińska M, Witeska M, Grużewska A, et al. The prevalence of virulence determinants in methicillin-resistant Staphylococcus aureus isolated from different infections in hospitalized patients in Poland. Scientific Reports. 2022;12(1):5477.
  10. Ahari H, Gharehbagh SS, Anvar A, Aftoum M, Khani M. Evaluation of a Multiplex Polymerase Chain Reaction for the Simultaneous Detection of Vibrio spp. in Vegetables and Water. Iranian Journal of Veterinary Medicine. 2020;14(4).
  11. Hashemi SH, Seifrabiei MA, Ahmadi S, Alikhani MY. Frequency of nasal carriage of Staphylococcus aureus and its antimicrobial resistance in Hamadan's medical students. Avicenna Journal of Clinical Medicine. 2012;19(3):36-40.
  12. Rahbar M, Yaghoobi M, Kia-Darbandsari B. Prevalence of nasal carriage of Staphylococcus aureus and susceptibility of isolates to methicillin and mupirocin among healthcare workers in an Iranian Hospital. Infection Control & Hospital Epidemiology. 2006;27(3):323-5.
  13. Khoddami E. A survey on nasal carriers of Staphylococcus aureus among hospital staff. Journal of Babol University of Medical Sciences. 2001;3(2):52-5.
  14. Ghasemian R, Najafi N, Makhlough A, Khademlou M. Frequency of nasal carriage of Staphylococcus aureus and its antimicrobial resistance pattern in patients on hemodialysis. 2010;4(3):218-22.
  15. Sharma S, Pal S, Negi V, Juyal D, Sharma M, Prakash R. Staphylococcus aureus including MRSA nasal carriage among hospital exposed and unexposed medical students. Journal of Family Medicine and Primary Care. 2020;9(9):4936.
  16. Goes ICRdS, Romero LC, Turra AJ, Gotardi MA, Rodrigues TFSdO, Santos LdO, et al. Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil. Revista do Instituto de Medicina Tropical de São Paulo. 2021;63:e14.
  17. Rai JR, Amatya R, Rai SK. Hand and nasal carriage of Staphylococcus aureus and its rate of recolonization among healthcare workers of a tertiary care hospital in Nepal. JAC-Antimicrobial Resistance. 2022;4(3):dlac051.
  18. Saud B, Khatri G, Amatya N, Paudel G, Shrestha V. Methicillin-Resistant and Biofilm-Producing Staphylococcus aureus in Nasal Carriage among Health Care Workers and Medical Students. Canadian Journal of Infectious Diseases and Medical Microbiology. 2023;8424486.
  19. Pourramezan N, Moghadam SO, Pourmand MR. Methicillin-resistant Staphylococcus aureus tracking spread among health-care workers and hospitalized patients in critical wards at a university hospital, Tehran, Iran. New microbes and new infections. 2019;27:29-35.
  20. Abdullahi A, Montaseri Z, Yazdanparast SS, Montaseri M. Colonization and Antibiotic Resistance of Nasal Staphylococcus Aureus among Healthcare Workers in Southwestern Iran: Occurrence of OS-MRSA. Journal of Advanced Biomedical Sciences. 2022;12(4):388-396.
  21. Moghaddam TS, Namaei MH, Afshar D, Yousefi M. High frequency of SCCmec type IV and multidrug-resistant SCCmec type I among hospital acquired methicillin-resistant Staphylococcus aureus isolates in Birjand Imam Reza Hospital, Iran. Iranian Journal of Microbiology. 2022;14(1):67.
  22. Goudarzi M, Fazeli M, Goudarzi H, Azad M, Seyedjavadi SS. Spa typing of Staphylococcus aureus strains isolated from clinical specimens of patients with nosocomial infections in Tehran, Iran. Jundishapur Journal of Microbiology. 2016;9(7).
  23. Abbasi Montazeri E, Khosravi AD, Khazaei S, Sabbagh A. Prevalence of methicillin resistance and superantigenic toxins in Staphylococcus aureus strains isolated from patients with cancer. BMC microbiology. 2021;21:1-9.
  24. Hashemizadeh Z, Hadi N, Mohebi S, Kalantar-Neyestanaki D, Bazargani A. Characterization of SCCmec, spa types and Multi Drug Resistant of methicillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran. BMC research notes. 2019;12(1):1-6.
  25. Latifpour M, Narimani T, Sadeghi A, Niakan M. Determination of Virulence Factors and Resistance Profile of Methicillin-Resistant Staphylococcus aureus Strains among Different Types of spa, agr, and SCCmec. BioMed Research International. 2022;5863310.
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