اخبار و اعلانات

 آمار

تعداد نشریات282
تعداد نشریات سازمان80
تعداد شماره‌ها3,168
تعداد مقالات35,197
تعداد مشاهده مقاله50,944,054
تعداد دریافت فایل اصل مقاله90,949,423
Hadi, Saeed, Ahmadabadi, Mohammad, Valizadeh Kamran, Rana. (1403). Efficient in Vitro Callus Induction, Regeneration and Shoot Multiplication Protocols for Stachys inflata subsp. caucasica (Stschegl.); A Rare Medicinal Plant. سامانه مدیریت نشریات علمی, 13(3), 570-576. doi: 10.22034/jmpb.2023.357500.1592
Saeed Hadi; Mohammad Ahmadabadi; Rana Valizadeh Kamran. "Efficient in Vitro Callus Induction, Regeneration and Shoot Multiplication Protocols for Stachys inflata subsp. caucasica (Stschegl.); A Rare Medicinal Plant". سامانه مدیریت نشریات علمی, 13, 3, 1403, 570-576. doi: 10.22034/jmpb.2023.357500.1592
Hadi, Saeed, Ahmadabadi, Mohammad, Valizadeh Kamran, Rana. (1403). 'Efficient in Vitro Callus Induction, Regeneration and Shoot Multiplication Protocols for Stachys inflata subsp. caucasica (Stschegl.); A Rare Medicinal Plant', سامانه مدیریت نشریات علمی, 13(3), pp. 570-576. doi: 10.22034/jmpb.2023.357500.1592
Hadi, Saeed, Ahmadabadi, Mohammad, Valizadeh Kamran, Rana. Efficient in Vitro Callus Induction, Regeneration and Shoot Multiplication Protocols for Stachys inflata subsp. caucasica (Stschegl.); A Rare Medicinal Plant. سامانه مدیریت نشریات علمی, 1403; 13(3): 570-576. doi: 10.22034/jmpb.2023.357500.1592

Efficient in Vitro Callus Induction, Regeneration and Shoot Multiplication Protocols for Stachys inflata subsp. caucasica (Stschegl.); A Rare Medicinal Plant

Journal of Medicinal plants and By-products
مقاله 10، دوره 13، شماره 3، آذر 2024، صفحه 570-576    اصل مقاله (752.14 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2023.357500.1592
نویسندگان
Saeed Hadi؛ Mohammad Ahmadabadi* ؛ Rana Valizadeh Kamran
Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
چکیده
Stachys inflata subsp. caucasica (Stschegl.) is an important medicinal plant, containing highly valuable compounds such as verbascoside, pinene, limonene, and myrcene. In this study, we report efficient in vitro callus induction and shoot regeneration protocols for S. stchschegleevii from two different explant sources of leaf and internode. The highest callus induction rate was observed in internode explants using MS medium complemented with 0.3 mg/l TDZ and 0.7 mg/l 2,4-D. The resulting calli were embryogenic with the ability to grow and maintain their structure on MS medium containing 1.5 mg/l BAP at high efficiencies (90%). The β-gulucoronidase (gus) gene was then successfully transferred to the internode explants by the particle gun method, demonstrating their potential to express foreign genes. In conclusion, here we report efficient protocols for callus induction and regeneration of S. inflata subsp. caucasica that can be used in germplasm maintenance, production of secondary metabolites in in vitro cultures, and molecular breeding through genetic engineering.
کلیدواژه‌ها
Stachys schtschegleevii؛ In vitro regeneration؛ Micro-propagation؛ Lamiaceae؛ Shoot multiplication
مراجع
  1. Bilusic Vundac V. Taxonomical and phytochemical characterisation of 10 Stachys Taxa recorded in the Balkan Peninsula Flora: A review. Plants (Basel). 2019;8(2).
  2. Tomou E.M., Barda C., Skaltsa H. Genus Stachys: A review of traditional uses, phytochemistry and bioactivity. Medicines (Basel). 2020;7(10).
  3. Piozzi F., Bruno M. Diterpenoids from roots and aerial parts of the genus Stachys. Rec. Nat. Prod. 2011;5(1):1.
  4. Fay M.F. Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev Biol Plant. 1992;28(1):1-4.
  5. Kumar N., Reddy M. In vitro plant propagation: A review. J For Environ Sci. 2011;27.
  6. Firn R.D., Sharma N., Digby J. Physiology, growth and development of plants and cells in culture — the way ahead. In: Lumsden PJ, Nicholas JR, Davies WJ (eds.) Physiology, Growth and Development of Plants in Culture. Springer-Verlag. 1994.
  7. Loyola-Vargas V.M., Ochoa-Alejo N. An introduction to plant tissue culture: advances and perspectives. In: Loyola-Vargas VM, Ochoa-Alejo N (eds.) Plant cell culture protocols. Springer-Verlag. 2018.
  8. Rad A.C., Atri M., Mohsenzadeh F., Jahandideh E. Chromosome counts in Stachys inflata Benth. (Laminoideae): Chromosome number variation in different populations from Iran. Chromosom Bot. 2012;7(2):67-71.
  9. Maleki F., Valilou M.M.S. Poulk plant (Stachys schtschegleevii) and its antibacterial specifications. Asian J Res Bot. 2020;2(4):1-13.
  10. Stocker T.F., Qin D., Plattner G.K., Alexander L.V., Allen S.K., Bindoff N.L., Bréon F.M., Church J.A., Cubasch U., Emori S. Technical summary. Climate change 2013: the physical science basis Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change: Cambridge University Press; 2013. p. 33-115.
  11. Clem K.R., Fogt R.L., Turner J., Lintner B.R., Marshall G.J., Miller J.R., Renwick J.A. Record warming at the South Pole during the past three decades. Nat Clim Change. 2020;10(8):762-70.
  12. Maikhuri R., Phondani P., Dhyani D., Rawat L., Jha N., Kandari L. Assessment of climate change impacts and its implications on medicinal plants-based traditional healthcare system in Central Himalaya, India. Iran J Sci Technol Trans A Sci. 2018;42:1827-35.
  13. Nasrollahi S., Ghoreishi S.M., Ebrahimabadi A.H., Khoobi A. Gas chromatography-mass spectrometry analysis and antimicrobial, antioxidant and anti-cancer activities of essential oils and extracts of Stachys inflata subsp. caucasica plant as biological macromolecules. Int J Biol Macromol. 2019;128:718-23.
  14. Hosoki T., Yasufuku T. (eds.) In vitro mass-propagation of Chinese Artichoke (Stachys sieboldii Miq.). International Society for Horticultural Science (ISHS), Leuven, Belgium. 1992.
  15. Li W., Gao H.H., Lu R., Guo G.Q., Zheng G.C. Direct plantlet regeneration from the tuber of Stachys sieboldii. Plant Cell Tissue Organ Cult (PCTOC). 2002;71(3):259-62.
  16. Legkobit M.P., Khadeeva N.V. Variation and morphogenetic characteristics of different Stachys species during microclonal propagation. Russ J Genet. 2004;40(7):743-50.
  17. Mantovska D.I., Kapchina V.M., Yordanova Z.P. In vitro propagation of the Balkan endemic species Stachys leucoglossa Griseb. Bulg J Agric Sci. 2019;25(6):1211–5.
  18. Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473-97.
  19. Jefferson R.A., Kavanagh T.A., Bevan M.W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987;6(13):3901-7.
  20. Timmermans M.C.P., Malig P., Viera J., Messing J. The pFF plasmids: Cassettes utilising CaMV sequences for expression of foreign genes in plants. J Biotechnol. 1990;14:333-44.
  21. Ganapathi T.R., Nataraja K. Effect of auxins and cytokinins on plant regeneration from hypocotyls and cotyledons in niger (Guizotia abyssinica Cass.). Biol Plant. 1993;35(2):209-15.
  22. Bekut Hitl M., Brkic S., Kladar N., Dragovic G., Gavarić N., Bozin B. Potential of selected Lamiaceae plants in anti(retro)viral therapy. Pharmacol Res. 2017;133.
  23. Ozudogru E., Kaya E., Kırdök E., Issever Ozturk S. In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cell Dev Biol - Plant. 2011;47:309-20.
  24. Akter K., Hoque M.A. In vitro shoot regeneration of mint (Mentha sp. L.) Using different types of explants and levels of benzylaminopurine. Bangladesh J Agric Res. 2018;43:703-16.
  25. Özkum D., Tipirdamaz R. (eds.) Effects of different nutrien t media and explant types on in vitro shoot regeneration of medical species of Origanum minutiflorum O. Schwarz & Davis. International Society for Horticultural Science (ISHS), Leuven, Belgium. 2007.
  26. Verma S.K., Sahin G., Das A.K., Gurel E. In vitro plant regeneration of Ocimum basilicum L. is accelerated by zinc sulfate. In Vitro Cell Dev Biol - Plant. 2016;52(1):20-7.
  27. Jan T., Khatoon K. In vitro regeneration of Salvia santolinifolia. Pak J Bot. 2014;46:325-8.
  28. Virginia S., Eleni M. In vitro propagation of Satureja thymbra L. (Lamiaceae): A valuable aromatic-medicinal native plant of the Mediterranean region. GSC Biol Pharm Sci. 2019;9:009-20.
  29. Yating L. Callus induction and plant regeneration from Rosemary leaves. Bioscience Methods. 2012.
  30. Kour B., Azhar M., Kaul S., Dhar M.K. In vitro regeneration and mass multiplication of Prunella vulgaris L. Natl Acad Sci Lett. 2014;37:81-6.
  31. Hosseini B., Alizadeh M., Hassani A., Jafari M., Rahimi A. High-frequency in vitro direct shoot regeneration from nodal explants of Hyssop plant (Hyssopus officinalis L.). J Medicinal Plants By- Products (JMPB). 2016;2:187-93.
  32. Meftahizade H., Moradkhani H., Naseri B., Lofti M., Naseri A. Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes. J Med Plant Res. 2010;4.
  33. Ahmadabadi M., Ruf S., Bock R. A leaf-based regeneration and transformation system for maize (Zea mays L.). Transgenic Res. 2007;16(4):437-48.
  34. Khadem A., Moshtaghi N., Bagheri A. Regulatory networks of hormone-involved transcription factors and their downstream pathways during somatic embryogenesis of Arabidopsis thaliana. 3 Biotech. 2023;13(5):1-11.
  35. Schaller G.E., Bishopp A., Kieber J.J. The yin-yang of hormones: cytokinin and auxin interactions in plant development. Plant Cell. 2015;27(1):44-63.
  36. Sugimoto K., Gordon S.P., Meyerowitz E.M. Regeneration in plants and animals: dedifferentiation, transdifferentiation, or just differentiation? Trends Cell Biol. 2011;21(4):212-8.
  37. Sugimoto K., Meyerowitz E.M. Regeneration in Arabidopsis tissue culture. Methods Mol Biol. 2013;959:265-75.
  38. Weatherhead M.A., Burdon J., Henshaw G.G. Effects of activated charcoal as an additive plant tissue culture media: Part 2. Zeitschrift für Pflanzenphysiol. 1979;94(5):399-405.
  39. Danova K., Stanoeva J.P., Aneva I., Alipieva K., Stefova M. Plant growth regulators and activated charcoal selectively affect phenylethanoid and flavone glycoside accumulation in Sideritis scardica Griseb. Tissue Culture. Plants (Basel). 2023;12(13).
  40. Yildiz M. The prerequisite of the success in plant tissue culture: high frequency shoot regeneration. In: Leva A (ed.) Recent Advances in Plant in vitro Culture, IntechOpen. 2012.
آمار
تعداد مشاهده مقاله: 691
تعداد دریافت فایل اصل مقاله: 817


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب