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Analysis of phylogenetic relationships of Potamogeton and Stuckenia (Potamogetonaceae) in Iran based on morphological, anatomical and molecular data | ||
| The Iranian Journal of Botany | ||
| مقاله 2، دوره 29، شماره 2، اسفند 2023، صفحه 106-121 اصل مقاله (704.45 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2024.130476 | ||
| نویسندگان | ||
| Shabnam Abbasi1؛ Saeed Afsharzadeh* 2؛ Mehri Dinarvand3؛ Ludwig Triest4 | ||
| 1Department of Biology Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran | ||
| 2Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran | ||
| 3Forests and Rangelands Research Division, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran | ||
| 4Laboratory of Plant Biology and Nature Management (APNA), Department of Biology, Vrije Universiteit Brussel (VUB), Brussels, Belgium | ||
| چکیده | ||
| Potamogeton and Stuckenia comprise 90 and 7 respectively accepted species worldwide. Because of a wide range of intraspecific morphological variability including extensive phenotypic plasticity and ecological diversity, Potamogeton and Stuckenia are considered notoriously difficult taxa. A total of 100 accessions representing 11 taxa of Potamogeton and three of Stuckenia were collected across Iran. An initial screening based on allele lengths of highly variable cpDNA sequences allowed considering 59 different accessions of one to eight individuals of each species for further morphological, anatomical, and molecular analyses. NrDNA ITS and three plastid regions (rbcL, matK, and trnH-psbA) were employed to reconstruct molecular phylogenies using maximum likelihood and Bayesian inferences. Analyses of nrDNA ITS sequences generated well-resolved tree topology than plastid data. There was some incongruence between nuclear data and concatenated chloroplast marker data (P=0.001). Additional testing of ISSR and SRAP markers for 48 specimens showed higher resolution in species delimitation among linear leaved taxa, though not conclusive. Anatomical features could not separate Iranian species alone and should be used in combination with morphological characters that were highly informative. The results showed that Iranian Potamogeton species contain a rich gene pool due to the specific and diverse geographical conditions of Iran. | ||
| کلیدواژهها | ||
| aquatic plants؛ chloroplast markers؛ hybridization؛ ITS؛ molecular trees | ||
| عنوان مقاله [English] | ||
| تجزیه و تحلیل روابط فیلوژنتیکی Potamogeton و Stuckenia (Potamogetonaceae) در ایران بر اساس دادههای مورفولوژیکی، تشریحی و مولکولی | ||
| نویسندگان [English] | ||
| شبنم عباسی1؛ سعید افشارزاده2؛ مهری دیناروند3؛ لودویک تریست4 | ||
| 1گروه آموزش زیستشناسی، دانشگاه فرهنگیان، صندوق پستی ۸۸۹-۱۴۶۶۵ تهران، ایران | ||
| 2گروه زیستشناسی گیاهی و جانوری، دانشکده علوم و فناوریهای زیستی، دانشگاه اصفهان، اصفهان، ایران | ||
| 3مرکز تحقیقات کشاورزی و منابعطبیعی خوزستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران | ||
| 4آزمایشگاه زیستشناسی گیاهی و مدیریت طبیعت، دانشکده زیستشناسی وریج، دانشگاه بروکسل، بلژیک | ||
| چکیده [English] | ||
| Potamogeton و Stuckenia به ترتیب شامل 90 و 7 گونه پذیرفته شده در سراسر جهان هستند. به دلیل طیف وسیعی از تنوعات مورفولوژیکی درون گونهای از جمله انعطافپذیری فنوتیپی گسترده و تنوع زیست محیطی،Potamogeton و Stuckenia جنسهای بسیار دشواری در نظر گرفته میشوند. در مجموع 100 نمونه به نمایندگی از 11 گونه از Potamogeton و سه گونه از Stuckenia از سراسر ایران جمعآوری شدند. غربالگری اولیه بر اساس طول آلل توالیهایcpDNA ، امکان در نظر گرفتن 59 نمونه مختلف از یک تا هشت فرد از هر گونه را برای آنالیزهای مورفولوژیکی، تشریحی و مولکولی بیشتر فراهم نمود. توالیهای هستهای و کلروپلاستی برای بازسازی درختان مولکولی با استفاده از RaxML و رویکرد بیزین استفاده شدند. آنالیزهای nrDNA وضوح بالاتری را نسبت به دادههای کلروپلاستی، در واگرایی گونهها نشان دادند. تعدادی ناهماهنگی بین دادههای هستهای و کلروپلاستی وجود داشت (P=0.001). آنالیزهای اضافهتر نشانگرهای ISSR و SRAP برای 48 نمونه، وضوح بالاتری را در تعیین حدود گونهها در میان گونههای برگ خطی نشان داد، اگرچه قطعی نیست. ویژگیهای تشریحی نمیتوانند گونههای ایرانی Potamogeton را بهتنهایی تفکیک کند و باید در ترکیب با خصوصیات ریختشناسی که بسیار دربردارنده اطلاعات بودند استفاده شود. نتایج نشان دادند که گونههای Potamogeton ایرانی با توجه به شرایط خاص و متنوع جغرافیایی ایران دارای خزانه ژنتیکی غنی هستند. | ||
| کلیدواژهها [English] | ||
| گیاهان آبزی, نشانگرهای مولکولی, هیبریداسیون, ITS, درختان مولکولی | ||
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