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Eshaghniya, Alireza, Haghbin Nazarpak, Hadi, Ghalyanchilangeroudi, Arash, Hosseini, Hossein. (1403). Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran. سامانه مدیریت نشریات علمی, 79(3), 575-586. doi: 10.32592/ARI.2024.79.3.575
Alireza Eshaghniya; Hadi Haghbin Nazarpak; Arash Ghalyanchilangeroudi; Hossein Hosseini. "Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran". سامانه مدیریت نشریات علمی, 79, 3, 1403, 575-586. doi: 10.32592/ARI.2024.79.3.575
Eshaghniya, Alireza, Haghbin Nazarpak, Hadi, Ghalyanchilangeroudi, Arash, Hosseini, Hossein. (1403). 'Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran', سامانه مدیریت نشریات علمی, 79(3), pp. 575-586. doi: 10.32592/ARI.2024.79.3.575
Eshaghniya, Alireza, Haghbin Nazarpak, Hadi, Ghalyanchilangeroudi, Arash, Hosseini, Hossein. Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran. سامانه مدیریت نشریات علمی, 1403; 79(3): 575-586. doi: 10.32592/ARI.2024.79.3.575

Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran

Archives of Razi Institute
مقاله 17، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 575-586    اصل مقاله (1.13 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.575
نویسندگان
Alireza Eshaghniya1؛ Hadi Haghbin Nazarpak* 2؛ Arash Ghalyanchilangeroudi3؛ Hossein Hosseini4
1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran.
3Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
چکیده
Infectious bronchitis virus (IBV) has a variety of serotypes that cause many problems in the poultry industry. Two H120 and H120-D274 live vaccines were evaluated against strain IS/1494/06 (variant 2) infectious bronchitis virus challenge. The study aimed to determine whether it is possible to achieve success in controlling disease symptoms and pathological lesions and reducing virus shedding by combining two types of vaccines against different severities of the Poultry Infectious Bronchitis Virus. After random selection of 100 SPF chickens, four 25 chicks/group experimental groups were arranged in the H120 (IB-H120, Intervet®; Serial No: **** at 1st day + Booster 14th day + Challenged with IS/1494/06- 14 DPV), H120-D274 (Poulvac® IB Primer at 1st day + Booster 14th day + Challenged with IS/1494/06- 14 DPV), Control (No vaccine and No Challenge), and Challenged (No vaccine + Challenge). After euthanasia of the animals on the 42nd day of the research, serum neutralization (days 14, 28, and 42), ELISA (14 days after the booster vaccine and before challenged), Ciliostasis (7 days after the booster vaccine and seven days after challenged) and pathological lesion scores were evaluated on day 14 after being infected with IS/1494/06 type 2 strain (day 42). Also, the virus shedding was monitored by real-time RT-PCR. Infection with the IS/1494/06 variant2 strain showed high to moderate ciliostasis and pathologic scores. All groups that received the IB-H120-D274 vaccine had fewer lesions relatively. The IB-H120-D274 vaccinated groups showed the highest protection rates and high protection scores (70.3) compared to the IB-H120 vaccine groups (24.4). The virus shedding was significantly reduced in groups that received the H120-D274 vaccine compared to those that received the IB-H120 vaccine. In conclusion, the homologous IB-H120-D274 vaccine showed superior results compared to the IB-H120 vaccine.
کلیدواژه‌ها
IBV؛ combined vaccine؛ Serum neutralization test؛ ELISA؛ Real-time PCR
عنوان مقاله [English]
بررسی مقایسه ای مولکولی، سرولوژی و پاتولوژی واکسن ترکیبی IB-H120-D274 و Mass IB-H120 در برابر ویروس های برونشیت عفونی گونه 2 پرندگان جدا شده در ایران
چکیده [English]
ویروس برونشیت عفونی (IBV) دارای سروتیپ های مختلفی است که باعث ایجاد مشکلات زیادی در صنعت طیور می شود. دو واکسن زنده H120 و H120-D274 در برابر چالش ویروس برونشیت عفونی سویه IS/1494/06 (نوع 2) مورد ارزیابی قرار گرفتند. پس از انتخاب تصادفی 100 جوجه SPF، چهار گروه آزمایشی 25 جوجه/گروه در گروه های کنترل و چالش شده (بدون دریافت واکسن)، H120 (واکسینه شده علیه سویه IB-H120 در روز اول و سویه IB-793B در روز ۱۴) و H120-D274 (واکسینه شده علیه سویه های IB-H120-D274 در روز اول، و سویه IB-793B در روز 14) قرار گرفتند. همه گروه ها به جز گروه شاهد با سویه IS/1494/06 variant2 داخل چشمی در 14 روز پس از واکسیناسیون بوستر(روز 28) آلوده شدند. پس از اتانازی حیوانات در روز 42 تحقیق، آزمایشات خنثی سازی سرم (روزهای 14، 28 و 42)، الایزا (14 روز پس از واکسن بوستر، قبل از چالش)، سیلیوستاز (7 روز پس از واکسن بوستر و هفت روز پس از واکسن بوستر). به چالش کشیده شدند و نمرات ضایعه پاتولوژیک در روز 14 پس از آلوده شدن به سویه IS/1494/06 نوع 2 (روز 42) مورد ارزیابی قرار گرفت. همچنین، ریزش ویروس توسط RT-PCR بلادرنگ پایش شد. عفونت با سویه IS/1494/06 variant2 نمرات مژگانی و پاتولوژی بالا تا متوسط را نشان داد. تمام گروه هایی که واکسن IB-H120-D274 دریافت کردند ضایعات نسبتاً کمتری داشتند. گروه های واکسینه شده IB-H120-D274 بالاترین میزان حفاظت و امتیازات حفاظتی بالایی (70.3) را در مقایسه با گروه های واکسن IB-H120 (24.4) نشان دادند. ریزش ویروس در گروه هایی که واکسن H120-D274 را دریافت کرده بودند در مقایسه با گروه هایی که واکسن IB-H120 دریافت کردند به طور قابل معنی داری کاهش یافت. در نتیجه، واکسن همولوگ IB-H120-D274 نتایج بهتری را در مقایسه با واکسن IB-H120 نشان داد.
کلیدواژه‌ها [English]
IBV, واکسن ترکیبی, تست خنثی سازی سرم, الایزا, Real-time PCR
سایر فایل های مرتبط با مقاله
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