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Hakimzadeh, Sara, Kosar, Muberra. (1403). Wound healing activity of green synthesized copper nanoparticles through cell proliferation-migration, antimicrobial effects, and nitric oxide triggering. سامانه مدیریت نشریات علمی, 79(3), 639-644. doi: 10.32592/ARI.2024.79.3.639
Sara Hakimzadeh; Muberra Kosar. "Wound healing activity of green synthesized copper nanoparticles through cell proliferation-migration, antimicrobial effects, and nitric oxide triggering". سامانه مدیریت نشریات علمی, 79, 3, 1403, 639-644. doi: 10.32592/ARI.2024.79.3.639
Hakimzadeh, Sara, Kosar, Muberra. (1403). 'Wound healing activity of green synthesized copper nanoparticles through cell proliferation-migration, antimicrobial effects, and nitric oxide triggering', سامانه مدیریت نشریات علمی, 79(3), pp. 639-644. doi: 10.32592/ARI.2024.79.3.639
Hakimzadeh, Sara, Kosar, Muberra. Wound healing activity of green synthesized copper nanoparticles through cell proliferation-migration, antimicrobial effects, and nitric oxide triggering. سامانه مدیریت نشریات علمی, 1403; 79(3): 639-644. doi: 10.32592/ARI.2024.79.3.639

Wound healing activity of green synthesized copper nanoparticles through cell proliferation-migration, antimicrobial effects, and nitric oxide triggering

Archives of Razi Institute
مقاله 25، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 639-644    اصل مقاله (344.06 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.639
نویسندگان
Sara Hakimzadeh؛ Muberra Kosar*
Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, Mersin 10, Turkey
چکیده
The present experimental study was designed to evaluate the in vitro wound healing and anti-inflammatory effects of copper nanoparticles (CuNPs) green synthesized by the methanol extract Ferula macrecolea Boiss, as a plant with various pharmacological effects such as anti-inflammatory, and antimicrobial effects in traditional and modern medicine. The precipitation approach was used to green synthesis of CuNPs through mixing the methanol and copper sulfate solution. Cell viability and fibroblast proliferation assay were performed by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay. The migration abilities of fibroblasts cells were evaluated using a cell scratch in vitro wound healing assay. Effects of CuNPs on iNOS expression genes were also performed by Real-time PCR. In vitro antibacterial susceptibility test of CuNPs was carried out according to the standards protocol of the National Committee for Clinical Laboratory Standards (NCCLS). The analysis of SEM revealed that the green synthesized CNP exhibited a globular shape with a size ranging from 15-90 nm; while their majority were at 40-60 nm. The results of the MTT assay showed that the calculated CC50 value of green synthesized CuNPs was 236.3 μg/mL. The optimum concentrations of the CuNPs which were selected based on the 50% cytotoxic concentration (CC50) value; whereas dose-dependently increased the proliferation of fibroblast cells. The CuNPs dose-dependently increased the rate of wound closure after 16 and 24 h. The results of the Real-Time PCR showed that CuNPs causes an upregulation in expression level of gene of nitric oxide synthesis (iNOS) in RAW 264.7 cells. CuNPs showed the promising antimicrobial effects against S. aureus, S. epidermidis and Ps. aeruginosa. The present study showed the high potency of CuNPs green synthesized by F. macrecolea for wound healing through their antimicrobial, proliferation of fibroblast cells, and provoking iNOS.
کلیدواژه‌ها
In vitro؛ antibacterial؛ nanotechnology؛ treatment؛ wound
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مراجع
  1. Falanga V, Isseroff RR, Soulika AM, Romanelli M, Margolis D, Kapp S, Granick M, Harding K. Chronic wounds. Nature Reviews Disease Primers. 2022;8(1):50.
  2. Gonzalez AC, Costa TF, Andrade ZD, Medrado AR. Wound healing-A literature review. Anais brasileiros de dermatologia. 2016;91:614-20.
  3. Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, Patel SB, Khalid L, Isseroff RR, Tomic-Canic M. Epithelialization in wound healing: a comprehensive review. Advances in wound care. 2014;3(7):445-64.
  4. Han G, Ceilley R. Chronic wound healing: a review of current management and treatments. Advances in therapy. 2017;34:599-610.
  5. Rajendran NK, Kumar SS, Houreld NN, Abrahamse H. A review on nanoparticle based treatment for wound healing. Journal of Drug Delivery Science and Technology. 2018;44:421-30.
  6. Ezhilarasu H, Vishalli D, Dheen ST, Bay BH, Srinivasan DK. Nanoparticle-based therapeutic approach for diabetic wound healing. Nanomaterials. 2020;10(6):1234.
  7. Salvo J, Sandoval C. Role of copper nanoparticles in wound healing for chronic wounds: literature review. Burns & Trauma. 2022;10:47.
  8. Alyousif MS, Al-Abodi HR, Almohammed H, Alanazi AD, Mahmoudvand H, Shalamzari MH, Salimikia I. Chemical composition, apoptotic activity, and antiparasitic effects of Ferula macrecolea essential oil against Echinococcus granulosus protoscoleces. Molecules. 2021;26(4):888.
  9. Mahmoudvand H, Yadegari JG, Khalaf AK, Hashemi MJ, Dastyarhaghighi S, Salimikia I. Chemical composition, antileishmanial, and cytotoxic effects Ferula macrecolea essential oil against Leishmania tropica. Parasite Epidemiology and Control. 2022;19:e00270.
  10. Sadeghi N, Sadeghi H, Mohan DN, Sepahvand A, Alizadeh A, Garavand S. Chemical composition, anti-fungal and cytotoxic effects of Ferula macrecolea essential oil against Candida albicans resistant and sensitive strains. Journal of Herbmed Pharmacology. 2023;12(2):228-32.
  11. Ebrahimi K, Shiravand S, Mahmoudvand H. Biosynthesis of copper nanoparticles using aqueous extract of Capparis spinosa fruit and investigation of its antibacterial activity. Marmara Pharmaceutical Journal. 2017;21(4):866-71.
  12. Albalawi AE, Abdel-Shafy S, Khudair Khalaf A, Alanazi AD, Baharvand P, Ebrahimi K, Mahmoudvand H. Therapeutic potential of green synthesized copper nanoparticles alone or combined with meglumine antimoniate (glucantime®) in cutaneous leishmaniasis. Nanomaterials. 2021;11(4):891.
  13. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods. 1983;65(1-2):55-63.
  14. Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. Journal of ethnopharmacology. 2009;126(3):463-7.
  15. Gharavi MJ, Nobakht M, Khademvatan SH, Bandani E, Bakhshayesh M, Roozbehani M. The effect of garlic extract on expression of INFγ and inos genes in macrophages infected with Leishmania major. Iranian Journal of Parasitology. 2011;6(3):74.
  16. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. Wayne, PA: The Clinical and Laboratory Standards Institute. 2012.
  17. Xu J, Song M, Fang Z, Zheng L, Huang X, Liu K. Applications and challenges of ultra-small particle size nanoparticles in tumor therapy. Journal of Controlled Release. 2023;353:699-712.
  18. Alizadeh S, Seyedalipour B, Shafieyan S, Kheime A, Mohammadi P, Aghdami N. Copper nanoparticles promote rapid wound healing in acute full thickness defect via acceleration of skin cell migration, proliferation, and neovascularization. Biochemical and biophysical research communications. 2019;517(4):684-90.
  19. Hu H, Tang Y, Pang L, Lin C, Huang W, Wang D, Jia W. Angiogenesis and full-thickness wound healing efficiency of a copper-doped borate bioactive glass/poly (lactic-co-glycolic acid) dressing loaded with vitamin E in vivo and in vitro. ACS applied materials & interfaces. 2018;10(27):22939-50.
  20. Wu M, Lu Z, Wu K, Nam C, Zhang L, Guo J. Recent advances in the development of nitric oxide-releasing biomaterials and their application potentials in chronic wound healing. Journal of Materials Chemistry B. 2021;9(35):7063-75.
  21. Malone‐Povolny MJ, Maloney SE, Schoenfisch MH. Nitric oxide therapy for diabetic wound healing. Advanced healthcare materials. 2019;8(12):1801210.
  22. Crisan MC, Teodora M, Lucian M. Copper nanoparticles: Synthesis and characterization, physiology, toxicity and antimicrobial applications. Applied Sciences. 2021;12(1):141.
  23. Betancourt-Galindo R, Reyes-Rodriguez PY, Puente-Urbina BA, Avila-Orta CA, Rodríguez-Fernández OS, Cadenas-Pliego G, Lira-Saldivar RH, García-Cerda LA. Synthesis of copper nanoparticles by thermal decomposition and their antimicrobial properties. Journal of Nanomaterials. 2014.
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