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Iranparast, Farideh, Farkhari, Mohammad, Eskandari, Ali. (1403). Effect of Genotype and Methyl Jasmonate on Silymarin Content of Silybum marianum (L.) Gaertn. Hairy Roots Culture. سامانه مدیریت نشریات علمی, 13(4), 941-946. doi: 10.22034/jmpb.2023.363593.1609
Farideh Iranparast; Mohammad Farkhari; Ali Eskandari. "Effect of Genotype and Methyl Jasmonate on Silymarin Content of Silybum marianum (L.) Gaertn. Hairy Roots Culture". سامانه مدیریت نشریات علمی, 13, 4, 1403, 941-946. doi: 10.22034/jmpb.2023.363593.1609
Iranparast, Farideh, Farkhari, Mohammad, Eskandari, Ali. (1403). 'Effect of Genotype and Methyl Jasmonate on Silymarin Content of Silybum marianum (L.) Gaertn. Hairy Roots Culture', سامانه مدیریت نشریات علمی, 13(4), pp. 941-946. doi: 10.22034/jmpb.2023.363593.1609
Iranparast, Farideh, Farkhari, Mohammad, Eskandari, Ali. Effect of Genotype and Methyl Jasmonate on Silymarin Content of Silybum marianum (L.) Gaertn. Hairy Roots Culture. سامانه مدیریت نشریات علمی, 1403; 13(4): 941-946. doi: 10.22034/jmpb.2023.363593.1609

Effect of Genotype and Methyl Jasmonate on Silymarin Content of Silybum marianum (L.) Gaertn. Hairy Roots Culture

Journal of Medicinal plants and By-products
مقاله 4، دوره 13، شماره 4، اسفند 2024، صفحه 941-946    اصل مقاله (761.11 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2023.363593.1609
نویسندگان
Farideh Iranparast1؛ Mohammad Farkhari* 1؛ Ali Eskandari2
1Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Khuzestan, Iran
2Nuclear Agriculture Group, Nuclear Science and Technology Research Institute, Karaj, Iran
چکیده
Milk thistle is a valuable medicinal plant, and obtaining silymarin (SLM) is the main reason for the cultivation of this plant. Due to the non-agricultural properties of milk thistle, its cultivation and harvesting are accompanied by difficulties. In vitro producing SLM by hairy root culture can overcome these problems. Various factors such as plant genotype, Agrobacterium rhizogenes strain used for infection, type and concentration of elicitor and, etc., can significantly enhance SLM production. The impact of various genotypes on SLM production in milk thistle hairy root culture has yet to be investigated. In this study examined the effect of five different genotypes of milk thistle and methyl jasmonate (MeJA) on the amount of SLM in milk thistle hairy roots based on completely randomized design. HPLC was used to quantify the amount of SLM in the hairy root samples. Based on the results, SLM production showed a considerable difference among ecotypes, with levels ranging from 109.8 to 648.7 ppm. MeJA increased the amount of SLM in all genotypes except for Mashhad ecotype. Due to the approximately 5.9-fold difference in SLM levels among different genotypes and the opposite responses of genotypes to MeJA indicate a high variation in this trait among different milk thistle ecotypes. Based on these results, screening milk thistle germplasm to find ecotypes with the highest levels of SLM in hairy root culture and also considering the strong mutual effect between elicitor and ecotype can be fruitful for increasing SLM content.
کلیدواژه‌ها
Diversity؛ Methyl jasmonate Milk thistle؛ Secondary metabolite
مراجع
  1. Abenavoli L., Capasso R., Milic N., Capasso F. Milk thistle in liver diseases: past, present, future. Phytotherapy Res. 2010; 24: 1423-1432.
  2. Devi K.P., Malar D.S., Braidy N., Nabavi S.M., Nabavi S.F. A Mini Review on the Chemistry and Neuroprotective Effects of Silymarin. Current Drug Targets. 2017; 18: 1529-1536.
  3. Kim J.L., Kim Y.H., Kang M.K., Gong J.H., Han S.J., Kang Y.H. Antiosteoclastic activity of milk thistle extract after ovariectomy to suppress estrogen deficiency-induced osteoporosis. BioMed Res. Int. 2013, pp. 1-11.
  4. Di Pierro F., Callegari A., Carotenuto D., Tapia M.M. Clinical efficacy, safety and tolerability of BIO-C (micronized Silymarin) as a galactagogue. Acta Biomed. 2008; 79: 205-210.
  5. Polachi N., Bai G., Li T., Chu Y., Wang X., Li S., Gu N., Wu J., Li W., Zhang Y., Zhou S., Sun H., Liu C. Modulatory effects of silibinin in various cell signaling pathways against liver disorders and cancer–A comprehensive review. European J. Med Chem. 2016; 123: 577-595.
  6. Khalili M., Hasanloo T., Tabar S.K.K. Ag+ enhanced silymarin production in hairy root cultures of Silybum marianum (L.) Gaertn. Plant Omics J. 2010; 3: 109-114.
  7. Hasanloo T., Eskandari S., Najafi F. Chitosan (middle-viscous) as an effective elicitor for silymarin production in Silybum marianum hairy root cultures. Res. J. Pharmacognosy. 2014; 1: 9-13.
  8. Hasanloo T., Sepehrifar R., Rahnama H., Shams M.R. Evaluation of the yeast-extract signaling pathway leading to silymarin biosynthesis in milk thistle hairy root culture. World J. Microbiology and Biotechnology. 2009; 25: 1901-1909.
  9. Gharechahi J., Khalili M., Hasanloo T., Salekdeh G.H. An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots. Plant Physiology and Biochem. 2013; 70: 115-122.
  10. Rahimi S., Hasanloo T., Najafi F., Khavari-Nejad R.A. Methyl jasmonate influence on silymarin production and plant stress responses in Silybum marianum hairy root cultures in a bioreactor. Natural Product Res. 2012; 26: 1662-1667.
  11. Khalili M., Hasanloo T., Tabar S.K.K., Rahnama H. Influence of exogenous salicylic acid on flavonolignans and lipoxygenase activity in the hairy root cultures of Silybum marianum. Cell Biology Int. 2009; 33: 988-994.
  12. Hasanloo T., Ahmadi M., Khayyam Nekoei S., Salehi Jouzani G. Improvement of silymarin production in hairy root cultures of Silybum marianum (L.) Gaertn using fungal elicitors. Rom Biotech Lett. 2013; 18: 8221-8232.
  13. Hasanloo T., Eskandari S., Kowsari M. Trichoderma strains-Silybum marianum hairy root cultures interactions. Res. J. Pharma. 2015; 2: 33-46.
  14. Nouri Y., Farkhari M. Silymarin Production in Inoculated Silybum marianum L. Hairy Roots Culture with Piriformospora indica. Russian J. Plant Physiology. 2023; 70: 1-8.
  15. Elateeq A.A., Sun Y., Nxumalo W., Gabr A.M. Biotechnological production of silymarin in Silybum marianum L.: A review. Biocatalysis and Agric. Biotechnology. 2020; 29: 1-16.
  16. Hamouda M. Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt. J. Genetic Engin. Biotechnology. 2019; 17: 1-9.
  17. Koohi-Dehkordi M., Rafizadeh A., Latifi E. Assessment of Genetic Diversity in Milk Thistle (Silybum marianum L.) Accessions Using SCoT and CDDP Markers. Seed and Plant J. 2018; 34: 63-80.
  18. Mohammadi S.A., Shokrpour M., Moghaddam M., Javanshir A. AFLP-based molecular characterization and population structure analysis of Silybum marianum L. Plant Genetic Resources. 2011; 9: 445-453.
  19. Rafizadeh A., Koohi-Dehkordi M., Sorkheh K. Molecular insights of genetic variation in milk thistle (Silybum marianum [L.] Gaertn.) populations collected from southwest Iran. Molecular Biology Reports. 2018; 45: 601-609.
  20. Saghalli A., Farkhari M., Salavati A., Alamisaeid K., Abdali A. Genetic diversity assessment of Milk Thistle (Silybum marianum L.) ecotypes using ISSR markers. Agric. Biotechnology J. 2016; 8: 51-64.
  21. Saghalli A., Farkhari M., Salavati A., Alamisaeid K., Abdali Mashhadi A. Evaluation of the genetic diversity of Silybum marianum L. ecotypes using yield components, morphological and phenological traits. Iranian J. Med. Aromatic Plants Res. 2018; 33: 990-1002.
  22. Zamani N., Mirzaei K., Zamani V. Evaluation of genetic diversity in several populations of medicinal Milk thistle using molecular marker. Cellular and Molecular Res. (Iranian J. Biology). 2018; 31: 210-221.
  23. Choi D.W., Jong J.D., Ha Y.I., Park H.W., In D.S., Chung H.J., Liu J.R. Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. Plant Cell Reports. 2005; 23: 557-566.
  24. Creelman R.A., Mullet J.E. Biosynthesis and action of jasmonates in plants. Annual Review of Plant Biology. 1997; 48: 355-381.
  25. Mittler R. ROS are good. Trends in Plant Sci. 2017; 22: 11-19.
  26. Zhao J., Davis L.C., Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Advances. 2005; 23: 283-333.
  27. Singh A., Dwivedi P. Methyl-jasmonate and salicylic acid as potent elicitors for secondary metabolite production in medicinal plants: A review. J. Pharma. Phytochem. 2018; 7: 750-757.
  28. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962; 15: 473-497.
  29. Cacho M., Morán M., Corchete P., Fernández-Tárrago J. Influence of medium composition on the accumulation of flavonolignans in cultured cells of Silybum marianum (L.) Gaertn. Plant Sci. 1999; 144: 63-68.
  30. Hsanlou T., Khavarinezhad R.A., Majidi H.E., Shams A.M. Analysis of flavonolignans in dried fruits of Silybum marianum (L.) Gaertn from Iran. Pakistan J. Biological Sci. 2005; 8: 1778-1782.
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