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In vitro and in vivo effects of green synthesized silver nanoparticles against Giardia lamblia infection | ||
| Archives of Razi Institute | ||
| مقاله 23، دوره 79، شماره 1، فروردین و اردیبهشت 2024، صفحه 218-225 اصل مقاله (456.22 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.1.218 | ||
| نویسندگان | ||
| Sara Golabi Azad* ؛ Hasip Cem Özyurt | ||
| Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus | ||
| چکیده | ||
| The current experimental study designed to examine the in vitro and in vivo effects of green synthesized silver nanoparticles (AgNPs) against G. lamblia as one of the main causes of parasitic diarrhea. The precipitation method was used to green synthesis of AgNPs by Astragalus ecbatanus aqueous extract. In vitro assay was performed on G. lamblia cysts and trophozoites after exposure with AgNPs at 10, 20, and 40 mg/mL for 10–360 min. Effects of AgNPs on trophozoite plasma membrane and their cytotoxic effects on normal and colon cancer cells were assessed by Sytox green and cell viability MTT assay. For thein vivo assay, BALB/c mice infected by Giardia, were treated with AgNPs at 10, 15, and 20 mg/kg/day for one week. On the 8th day post-infection, stool exam was performed to check the Giardia cysts presence and the reduction rate. The AgNPs were in the range between 5 and 80 nm, and the maximum distribution of particlesize was observed at 40–60 nm. AgNPs markedly (p<0.001) increased the mortality of G. lamblia trophpzoites as a dose-dependent reaction; AgNPs at 200 and 300 μg/mL destroyed G. lamblia cysts after 4 and 2 h, respectively. Trophozoites of G. lamblia were more sensitive to AgNPs than cysts, whereas, AgNPs at 100, 200, and 300 μg/mL killed all trophozoites after 4, 2, and 1 h of treatment, respectively. AgNPs dose-dependently dropped (p<0.001) the parasite load and viability of cysts of G. lamblia. Exposure of G. lamblia trophozoites with AgNPs dose-dependently increased the plasma membrane permeability of trophozoites via rising the exposed fluorescence. The CC50 value AgNPs for colon cancer and normal cell lines was 402.3 μg/mL and 819.6 μg/mL, respectively. The selectivity value greater than 2 (2.04) implies that this AgNPs is safe for normal cells compared to cancer cells. This experimental study showed that AgNPs green synthesized by A. ecbatanus displayed considerable in vitro and in vivo anti-Giardia activity and can accordingly a candidate for Giardia infection treatment. Nonetheless, farther researches on the accurate mechanisms of action as well as clarifying all toxicity aspects of the type of AgNPs must be considered. | ||
| کلیدواژهها | ||
| Giardiasis، treatment، cyst، trophozites؛ mice | ||
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| مراجع | ||
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References 1. DuPont HL. Persistent diarrhea: a clinical review. Jama. 2016 Jun 28;315(24):2712-23. 2. Steffen R. Epidemiology of travellers’ diarrhea. Journal of travel medicine. 2017 Apr 1;24(suppl_1):S2-5. 3. Hodges K, Gill R. Infectious diarrhea: Cellular and molecular mechanisms. Gut microbes. 2010 Jan 1;1(1):4- 21. 4. Hooshyar H, Rostamkhani P, Arbabi M, Delavari M. Giardia lamblia infection: review of current diagnostic strategies. Gastroenterology and Hepatology from bed to Bench. 2019;12(1):3. 5. Cernikova L, Faso C, Hehl AB. Five facts about Giardia lamblia. PLoS pathogens. 2018 Sep 27;14(9):e1007250. 6. Einarsson E, Ma’ayeh S, Svärd SG. An up-date on Giardia and giardiasis. Current opinion in microbiology. 2016 Dec 1;34:47-52. 7. Alnomasy S, Al-Awsi GR, Raziani Y, Albalawi AE, Alanazi AD, Niazi M, Mahmoudvand H. Systematic review on medicinal plants used for the treatment of Giardia infection. Saudi Journal of Biological Sciences. 2021 Sep 1;28(9):5391-402. 8. Lalle M, Hanevik K. Treatment-refractory giardiasis: challenges and solutions. Infection and drug resistance. 2018 Oct 10:1921-33. 9. Leitsch D. Drug resistance in the microaerophilic parasite Giardia lamblia. Current tropical medicine reports. 2015 Sep;2:128-35. 10. Lin W. Introduction: nanoparticles in medicine. Chemical reviews. 2015 Oct 14;115(19):10407-9. 11. Anselmo AC, Mitragotri S. Nanoparticles in the clinic. Bioengineering & translational medicine. 2016 Mar;1(1):10-29. 12. Kandeel M, Akhtar T, Zaheer T, Ahmad S, Ashraf U, Omar M. Anti-parasitic Applications of Nanoparticles: A Review. Pakistan Veterinary Journal. 2022 Apr 1;42(2). 13. Dos Santos CA, Seckler MM, Ingle AP, Gupta I, Galdiero S, Galdiero M, Gade A, Rai M. Silver nanoparticles: therapeutical uses, toxicity, and safety issues. Journal of pharmaceutical sciences. 2014 Jul 1;103(7):1931-44. 14. Zhang P, Gong J, Jiang Y, Long Y, Lei W, Gao X, Guo D. Application of Silver Nanoparticles in Parasite Treatment. Pharmaceutics. 2023 Jun 21;15(7):1783. 15. Albalawi AE, Abdel-Shafy S, Khudair Khalaf A, Alanazi AD, Baharvand P, Ebrahimi K, Mahmoudvand H. Therapeutic potential of green synthesized copper nanoparticles alone or combined with meglumine antimoniate (glucantime®) in cutaneous leishmaniasis. Nanomaterials. 2021 Mar 31;11(4):891. 16. Kharazmkia A, Yadegari JG, Mohammadian M, Ali R, Mahmoudvand H. Antiparasitic effect of limonene, a monoterpene compound found in the essential oils against Giardia lamblia. Journal of Herbmed Pharmacology. 2023 Mar 18;12(2):327-30. 17. Albalawi AE, Alanazi AD. Chemical Composition, In Vitro, and In Vivo Antiparasitic Effects of Curcuma zedoaria Rhizome Essential Oil Against Giardia lamblia. Pharmacognosy Magazine. 2023 Jun;19(2):418-26. 18. Ezzatkhah F, Khalaf AK, Mahmoudvand H. Copper nanoparticles: Biosynthesis, characterization, and protoscolicidal effects alone and combined with albendazole against hydatid cyst protoscoleces. Biomedicine & Pharmacotherapy. 2021 Apr 1;136:111257. 19. Albalawi AE, Khalaf AK, Alyousif MS, Alanazi AD, Baharvand P, Shakibaie M, Mahmoudvand H. Fe3O4@ piroctone olamine magnetic nanoparticles: Synthesize and therapeutic potential in cutaneous leishmaniasis. Biomedicine & Pharmacotherapy. 2021 Jul 1;139:111566. 20. Ghasemian Yadegari J, Khudair Khalaf A, Darabi R, Mahmoudvand H. Antiparasitic effects and cellular mechanism of Astragalus maximus chloroform extract against clinical isolates of Giardia lamblia. Res J Pharmacogn. 2022 Jul 1;9(3):5-13. 21. Deshmukh SP, Patil SM, Mullani SB, Delekar SD. Silver nanoparticles as an effective disinfectant: A review. Materials Science and Engineering: C. 2019 Apr 1;97:954- 65. 22. Srikar SK, Giri DD, Pal DB, Mishra PK, Upadhyay SN. Green synthesis of silver nanoparticles: a review. Green and Sustainable Chemistry. 2016 Feb 16;6(1):34-56. 23. Idan E, Ardalan N. Introducing Silver Nanoparticles as Anti-Giardial inExperimentally Infected Mice: Therapy versus Toxicity. Syst. Rev. Pharm. 2020;11:701-8. 24. Al‐Quraishy S, Murshed M, Delic D, Al‐Shaebi EM, Qasem MA, Mares MM, Dkhil MA. Plasmodium chabaudi‐infected mice spleen response to synthesized silver nanoparticles from Indigofera oblongifolia extract. Letters in Applied Microbiology. 2020 Nov 1;71(5):542-9. 25. Cameron P, Gaiser BK, Bhandari B, Bartley PM, Katzer F, Bridle H. Silver nanoparticles decrease the viability of Cryptosporidium parvum oocysts. Applied and environmental microbiology. 2016 Jan 15;82(2):431-7. | ||
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