| Obesity has been an important health concern over a decade, causing serious health issues worldwide. Treatments available for obesity include FDA approved drugs like Lorcaserin, Orlistat, Bupropion, combinations of Phentermine and Topiramate, Sibutramine but these have adverse effects on health. To address the issue the current study was performed on evaluating the anti-obesity potential of Phyllanthus fraternus leaves. These leaves are a rich source of different phytochemicals like alkaloids, saponins, terpenoids, tannins etc., and the plant has showed to exhibit medicinal properties hence it can be used in the treatment of obesity disorder. The crude extract of plants was prepared in three different solvents i.e., methanol, hydro alcohol and isopropyl alcohol. Determination of lipid inhibition was done using lipase inhibition assay and Amylase assay was carried out to determine if the plant extract possessed anti-diabetic properties. An Oil red staining was carried out to determine lipid accumulation in which the cells were incubated with plant extract for 48 hrs. In order to determine if the plant extract was toxic to 3T3 cells MTT assay was carried out to access cell viability. Through lipase inhibition assay we depicted potent anti-obesity property, isopropyl alcohol extract exhibited 67.45% inhibition at the concentration of 500µg/ml. Methanol extract showed highest percent of α amylase inhibition i.e., 90.03% at a concentration of 1000µg/ml. MTT assay concluded that the plant extracts were not cytotoxic to the cells at a concentration range between 20µg/ml to 100µg/ml the percentage of viable cells was 98% to 63%. The plant extract successfully could be used to treat obesity. The results obtained from the current study revealed that the plant exhibits potent anti-obesity properties. |
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