Phytochemical, Antioxidant and Antifungal Investigations of Vitex agnus-castus Leaf Essential Oil | ||
| مهار زیستی در گیاهپزشکی | ||
| Volume 10, Issue 2, December 2023, Pages 67-80 PDF (724.92 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/bcpp.2023.363132.343 | ||
| Authors | ||
| Faezeh Farokhzad1; Hossein Dehghan* 2; Seyed Abdollah Hashemi3; Kamal Gholamipour Fard4 | ||
| 1Department of Horticulture, Faculty of Agriculture, Shahed University, Tehran, Iran | ||
| 2Medicinal Plants Research Center, Shahed University, Tehran, Iran | ||
| 3Department of Plant Protection, Faculty of Agriculture, Shahed University, Tehran, Iran | ||
| 4Department of Plant Production, College of Agriculture and Natural Resources of Darab, Shiraz University, Darab, Iran | ||
| Abstract | ||
| Vitex agnus–castus, belonging to the Lamiaceae family, is used in traditional medicine in Iran. With the aim of finding natural compounds with anti–fungal and eco–friendly properties, the effect of Vitex agnus–castus essential oil on mycelial growth of three plant pathogenic fungi was evaluated. The study was conducted as factorial experiments with three fungal isolates, including Botrytis cinerea, Penicillium digitatum and Aspergillus flavus. The inhibitory activity of six doses of the essential oil (2, 4, 8, 16, 32 and 64 μl) with three replicates were examined. Also, the antioxidant effect of the oil was evaluated by DPPH free radical inhibition method. The chemical compounds of the oil were identified and quantified using GC–MS method. Analysis of the chemical composition of the essential oil showed that α–pinene (52.70%), limonene (15.77%) and (E)–caryophyllene (8.69%) constitute the most components of the essential oil, respectively. As results of the study showed that the 64 ml of V. agnus–castus essential oil has the greatest inhibitory effect on mycelial growth of studied fungi. Among the studied fungal species, P. digitatum showed the least and B. cinerea showed the most sensitivity to the of V. agnus–castus essential oil. Also, the essential oil was showed a high antioxidant activity with IC50 value of 0.07 mg/ml compared to the BHT (IC50 = 0.04 mg/mL), as a synthetic antioxidant. . | ||
| Keywords | ||
| Vitex agnus-castus; essential oil; antioxidant activity; chemical compounds; antifungal activity | ||
| References | ||
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