| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,533,301 |
| تعداد دریافت فایل اصل مقاله | 90,481,495 |
Specific up-regulation of transcription factors in the fungus Parastagonospora nodorum suggests a role in pathogenicity on wheat | ||
| Mycologia Iranica | ||
| دوره 10، شماره 2، اسفند 2023، صفحه 79-88 اصل مقاله (1.26 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/MI.2023.362598.1263 | ||
| نویسندگان | ||
| Roya Choupannejad1؛ Bahram Sharifnabi* 1؛ Rahim Mehrabi2؛ Javad Gholami3؛ Amir Massah3؛ Jerome Collemare4 | ||
| 1Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| 2Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| 3Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran. | ||
| 4Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands | ||
| چکیده | ||
| The necrotrophic fungus Parastagonospora nodorum, the causal agent of stagonospora nodorum blotch (SNB), is responsible for significant economic losses of wheat worldwide. Despite the presence of a high number of TF-encoding genes within the genome of P. nodorum, very little is known about their expression profile and their functions during cellular and biological processes. In this study, ten putative TF genes in P. nodorum isolate SN15 were selected, four of which shared homology with well-known fungal TFs involved in pathogenesis, and the other six genes were located in putative secondary metabolite biosynthetic gene clusters (BGCs). The expression profile of these candidate TF genes was investigated using both semi-quantitative and quantitative RT-PCR assays under in vitro and in planta conditions. The results revealed that six candidate TF genes exhibited the highest expression levels in the minimal media lacking both nitrogen and carbon sources as well as during the early stages of infection. These findings suggest that these six candidate TFs may play an important role in the pathogenicity of P. nodorum. | ||
| کلیدواژهها | ||
| Glume blotch؛ QPCR؛ Expression profile؛ Minimal media | ||
| مراجع | ||
|
Blin, K., Shaw, S., Augustijn, H.E., Reitz, Z.L., Biermann, F., Alanjary, M., Fetter, A., Terlouw, B.R., Metcalf, W.W., Helfrich, E.J.N., van Wezel, G.P., Medema, M.H. and Weber, T., 2023. antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation. Nucleic Acids Research 51:46-50.
Chen, L., Tong, Q., Zhang, C. and Ding, K., 2019. The transcription factor FgCrz1A is essential for fungal development, virulence, deoxynivalenol biosynthesis and stress responses in Fusarium graminearum. Current Genetics 65:153-166.
Chooi, Y.H., Muria-Gonzalez, M.J. and Solomon, P.S., 2014. A genome-wide survey of the secondary metabolite biosynthesis genes in the wheat pathogen Parastagonospora nodorum. Mycology 5:192-206.
Chooi, Y.H., Zhang, G., Hu, J., Muria‐Gonzalez, M.J., Tran, P.N., Pettitt, A., Maier, A.G., Barrow, R.A. and Solomon, P.S., 2017. Functional genomics‐guided discovery of a light‐activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation. Environmental Microbiology 19:1975-1986.
Donofrio, N., Oh, Y., Lundy, R., Pan, H., Brown, D., Jeong, J., Coughlan, S., Mitchell, T. and Dean, R., 2006. Global gene expression during nitrogen starvation in the rice blast fungus, Magnaporthe grisea. Fungal Genetics and Biology 43:605-617.
Duba, A., Goriewa-Duba, K. and Wachowska, U., 2018. A Review of the Interactions between Wheat and Wheat Pathogens: Zymoseptoria tritici, Fusarium spp. and Parastagonospora nodorum. International Journal of Molecular Sciences 19:1138.
Fang, Y., Xiong, D., Tian, L., Tang, C., Wang, Y. and Tian, C., 2017. Functional characterization of two bZIP transcription factors in Verticillium dahliae. Gene 626:386-394.
Gai, Y., Liu, B., Ma, H., Li, L., Chen, X., Moenga, S., Riely, B., Fayyaz, A., Wang, M. and Li, H., 2019. The methionine biosynthesis regulator AaMetR contributes to oxidative stress tolerance and virulence in Alternaria alternata. Microbiological Research 219:94-109.
Gamborg, O.L.C., Miller, R.A. and Ojima, K., 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50:151-158.
Hane, J.K., Lowe, R.G., Solomon, P.S., Tan, K.-C., Schoch, C.L., Spatafora, J.W., Crous, P.W., Kodira, C., Birren, B.W. and Galagan, J.E., 2007. Dothideomycete–plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum. The Plant Cell 19:3347-3368.
IpCho, S.V., Tan, K.-C., Koh, G., Gummer, J., Oliver, R.P., Trengove, R.D. and Solomon, P.S., 2010. The transcription factor StuA regulates central carbon metabolism, mycotoxin production, and effector gene expression in the wheat pathogen Stagonospora nodorum. Eukaryotic Cell 9:1100-1108.
John, E., Singh, K.B., Oliver, R.P. and Tan, K.C., 2021. Transcription factor control of virulence in phytopathogenic fungi. Molecular Plant Pathology 22:858-881.
Lin, S.-Y., 2017. The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum, PhD dissertation. The Australian National University. Australia.
Lin, S.Y., Chooi, Y.H. and Solomon, P.S., 2018. The global regulator of pathogenesis PnCon7 positively regulates Tox3 effector gene expression through direct interaction in the wheat pathogen Parastagonospora nodorum. Molecular Microbiology 109:78-90.
Liu, Z., Friesen, T., Rasmussen, J., Ali, S., Meinhardt, S. and Faris, J., 2004. Quantitative trait loci analysis and mapping of seedling resistance to Stagonospora nodorum leaf blotch in wheat. Phytopathology 94:1061-1067.
Mohammadi, N., Mehrabi, R., Gohari, A.M., Goltapeh, E.M., Safaie, N. and Kema, G.H., 2017. The ZtVf1 transcription factor regulates development and virulence in the foliar wheat pathogen Zymoseptoria tritici. Fungal Genetics and Biology 109:26-35.
Mosunova, O., Navarro-Muñoz, J.C. and Jérôme, C., 2021. The Biosynthesis of Fungal Secondary Metabolites: From Fundamentals to Biotechnological Applications. Encyclopedia of Mycology:458-476.
Oliver, R.P., Friesen, T.L., Faris, J.D. and Solomon, P.S., 2012. Stagonospora nodorum: from pathology to genomics and host resistance. Annual Review of Phytopathology 50:23-43.
Orek, C.O., 2018. An Optimized Cetyltrimethylammonium Bromide (CTAB)-Based Protocol for Extracting RNA from Young and Cassava Leaves. Journal of Advances in Biology & Biotechnology 19(4) 1-7.
Rahimi Tamandegani, P., Sharifnabi, B., Massah, A. and Zahravi, M., 2021. Induced reprogramming of oxidative stress responses in cucumber by Trichoderma asperellum (Iran 3062C) enhances defense against cucumber mosaic virus. Biological Control 164:104779.
Rybak, K., See, P.T., Phan, H.T., Syme, R.A., Moffat, C.S., Oliver, R.P. and Tan, K.C., 2017. A functionally conserved Zn2Cys6 binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host‐specific virulence of two major Pleosporales fungal pathogens of wheat. Molecular Plant Pathology 18:420-434.
Schmittgen, T.D. and Livak, K.J., 2008. Analyzing real-time PCR data by the comparative CT method. Nature Protocols 3:1101-1108.
Srivastava, A., Ohm, R.A., Oxiles, L., Brooks, F., Lawrence, C.B., Grigoriev, I.V. and Cho, Y., 2012. A zinc-finger-family transcription factor, AbVf19, is required for the induction of a gene subset important for virulence in Alternaria brassicicola. Molecular Plant-Microbe Interactions 25:443-452.
Thomma, B.P., Bolton, M.D., Clergeot, P.H. and De Wit, P.J., 2006. Nitrogen controls in planta expression of Cladosporium fulvum Avr9 but no other effector genes. Molecular Plant Pathology 7:125-130.
Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B.C., Remm, M. and Rozen, S.G., 2012. Primer3—new capabilities and interfaces. Nucleic Acids Research 40:e115-e115 | ||
|
آمار تعداد مشاهده مقاله: 729 تعداد دریافت فایل اصل مقاله: 522 |
||