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اثرات افزودن پودر خرفه(Portulace oleracea L.) با ویتامین E و ال –کارنیتین در جیره کم پروتئین بر ایمنی و فراسنجه‌های خونی در جوجه‌های گوشتی تحت تنش سرمایی | ||
| علوم دامی | ||
| دوره 36، شماره 139، شهریور 1402، صفحه 3-16 اصل مقاله (1.63 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2022.359102.2235 | ||
| نویسندگان | ||
| آزاده رستمیان* 1؛ امیرعلی صادقی2؛ احمد کریمی2 | ||
| 1دانشجوی دکتری تغذیه طیور، گروه علوم دامی، دانشگاه کردستان | ||
| 2استاد گروه علوم دامی، دانشگاه کردستان | ||
| چکیده | ||
| آزمایش حاضر برای بررسی اثرات افزودن پودر خرفه، ویتامین E و ال-کارنیتین در جیره‌های کم پروتئین بر سیستم ایمنی و فراسنجه‌های بیوشیمیایی خون در جوجه‌های گوشتی پرورش یافته در شرایط استرس سرمایی انجام شد. 576 جوجه گوشتی نر یک روزه (راس 308) به طور تصادفی به 6 گروه آزمایشی تقسیم شد که هر گروه آزمایشی دارای 6 تکرار و 16 پرنده در هر تکرار بود. گروه‌های آزمایشی شامل: (1) جیره با پروتئین معمول (شاهد منفی)، (2) جیره کم پروتئین (2 درصد کمتر نسبت به شاهد منفی) به عنوان شاهد، (3) جیره شاهد + 2 گرم بر کیلوگرم پودر خرفه، (4) جیره شاهد حاوی پودر خرفه + 250 میلی گرم برکیلوگرم ویتامین E ، (5) جیره شاهد حاوی پودر خرفه + 150 میلی گرم برکیلوگرم ال-کارنیتین و (6) جیره شاهد حاوی پودر خرفه + مخلوط ویتامین E و ال-کارنیتین بودند. نتایج نشان داد که افزودن پودر خرفه + ویتامین E و پودر خرفه + ال-کارنیتین به جیره شاهد شمار گلبول‌های قرمز خون، هموگلوبین، هماتوکریت و نسبت هتروفیل به لنفوسیت را در 42 روزگی کاهش دادند (05/0< P). تیتر آنتی بادی علیه گلبول‌های قرمز گوسفندی و ویروس بیماری نیوکاسل به ترتیب در روزهای 41 و 27 توسط ویتامین E و ال-کارنیتین افزایش یافت(05/0< P)، در حالی که میزان تری‌گلیسرید و کلسترول نیز توسط آن‌ها کاهش پیدا کرد(05/0< P). افزودن ویتامین E و ال-کارنیتین به تنهایی در ترکیب با خرفه در جیره کم پروتئین با ایجاد اثرات سینرژیک می‌تواند موجب بهبود سیستم ایمنی و سلامت در جوجه‌های گوشتی تحت استرس سرمایی شود. | ||
| کلیدواژه‌ها | ||
| تنش سرمایی؛ تیترآنتی‌بادی؛ جیره‌های کم پروتئین؛ هموگلوبین | ||
| عنوان مقاله [English] | ||
| The effects of adding purslane powder (Portulace oleracea L.) with vitamin E and L-carnitine in low protein diet on immunity and blood parameters in broilers under cold stress. | ||
| نویسندگان [English] | ||
| Azadeh Rostamian1؛ Amirali Sadeghi2؛ Ahmad Karimi2 | ||
| 1department of animal science, university of Kurdistan | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Kurdistan,, Sanandaj, Iran | ||
| چکیده [English] | ||
| This study was carried out to evaluate the effects of vitamin E (VtiE) and L-carnitine (L-Car) supplements with purslane powder (PP) in reduced-protein diet to immune response, hematological indices and some serum indices in broiler chickens reared at cold stress . 576 one-day-old male broiler chicks (Ross 308) were randomly assigned to six treatments, with six replicates per treatment and 16 birds per replicate. The experimental treatments were as follows: 1) normal-protein diet (negative control; NC), 2) a reduced- protein diet (20 g kg-1 lower protein) as a control diet (C), 3) C + 2 g kg-1 of PP, 4) C + PP diet supplemented with 250 mg kg-1 VitE, 5) C + PP diet supplemented with 150 mg kg-1 of L-Car and, 6) C + PP diet supplemented with 250 mg kg-1 VitE + 150 mg kg-1 L-Car. Results showed that diets supplemented with PP + VtiE and PP + L-Car showed decreased the number of red blood cells, hemoglobin, hematocrit and heterophil to lymphocyte ratio (P<0.05) at 42 days. Antibody titers against Sheep red blood cells and Newcastle disease virus were significantly increased (P<0.05) at 41 and 27 days by VtiE and L-Car, respectively. While triglycerides and cholesterol were significantly reduced by them (P<0.05). It could be concluded that addition of VtiE and L-Car alone in combination with purslane in low protein diets by create beneficial synergistic effects, it can improve immune system and health in broilers reared at cold stress. | ||
| کلیدواژه‌ها [English] | ||
| Cold stress, Antibody titer, Reduced- protein diet, Hemoglobin | ||
| مراجع | ||
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