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اثرات افزودن پودر خرفه(Portulace oleracea L.) با ویتامین E و ال –کارنیتین در جیره کم پروتئین بر ایمنی و فراسنجه‌های خونی در جوجه‌های گوشتی تحت تنش سرمایی | ||
| علوم دامی | ||
| دوره 36، شماره 139، شهریور 1402، صفحه 3-16 اصل مقاله (1.63 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2022.359102.2235 | ||
| نویسندگان | ||
| آزاده رستمیان* 1؛ امیرعلی صادقی2؛ احمد کریمی2 | ||
| 1دانشجوی دکتری تغذیه طیور، گروه علوم دامی، دانشگاه کردستان | ||
| 2استاد گروه علوم دامی، دانشگاه کردستان | ||
| چکیده | ||
| آزمایش حاضر برای بررسی اثرات افزودن پودر خرفه، ویتامین E و ال-کارنیتین در جیره‌های کم پروتئین بر سیستم ایمنی و فراسنجه‌های بیوشیمیایی خون در جوجه‌های گوشتی پرورش یافته در شرایط استرس سرمایی انجام شد. 576 جوجه گوشتی نر یک روزه (راس 308) به طور تصادفی به 6 گروه آزمایشی تقسیم شد که هر گروه آزمایشی دارای 6 تکرار و 16 پرنده در هر تکرار بود. گروه‌های آزمایشی شامل: (1) جیره با پروتئین معمول (شاهد منفی)، (2) جیره کم پروتئین (2 درصد کمتر نسبت به شاهد منفی) به عنوان شاهد، (3) جیره شاهد + 2 گرم بر کیلوگرم پودر خرفه، (4) جیره شاهد حاوی پودر خرفه + 250 میلی گرم برکیلوگرم ویتامین E ، (5) جیره شاهد حاوی پودر خرفه + 150 میلی گرم برکیلوگرم ال-کارنیتین و (6) جیره شاهد حاوی پودر خرفه + مخلوط ویتامین E و ال-کارنیتین بودند. نتایج نشان داد که افزودن پودر خرفه + ویتامین E و پودر خرفه + ال-کارنیتین به جیره شاهد شمار گلبول‌های قرمز خون، هموگلوبین، هماتوکریت و نسبت هتروفیل به لنفوسیت را در 42 روزگی کاهش دادند (05/0< P). تیتر آنتی بادی علیه گلبول‌های قرمز گوسفندی و ویروس بیماری نیوکاسل به ترتیب در روزهای 41 و 27 توسط ویتامین E و ال-کارنیتین افزایش یافت(05/0< P)، در حالی که میزان تری‌گلیسرید و کلسترول نیز توسط آن‌ها کاهش پیدا کرد(05/0< P). افزودن ویتامین E و ال-کارنیتین به تنهایی در ترکیب با خرفه در جیره کم پروتئین با ایجاد اثرات سینرژیک می‌تواند موجب بهبود سیستم ایمنی و سلامت در جوجه‌های گوشتی تحت استرس سرمایی شود. | ||
| کلیدواژه‌ها | ||
| تنش سرمایی؛ تیترآنتی‌بادی؛ جیره‌های کم پروتئین؛ هموگلوبین | ||
| عنوان مقاله [English] | ||
| The effects of adding purslane powder (Portulace oleracea L.) with vitamin E and L-carnitine in low protein diet on immunity and blood parameters in broilers under cold stress. | ||
| نویسندگان [English] | ||
| Azadeh Rostamian1؛ Amirali Sadeghi2؛ Ahmad Karimi2 | ||
| 1department of animal science, university of Kurdistan | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Kurdistan,, Sanandaj, Iran | ||
| چکیده [English] | ||
| This study was carried out to evaluate the effects of vitamin E (VtiE) and L-carnitine (L-Car) supplements with purslane powder (PP) in reduced-protein diet to immune response, hematological indices and some serum indices in broiler chickens reared at cold stress . 576 one-day-old male broiler chicks (Ross 308) were randomly assigned to six treatments, with six replicates per treatment and 16 birds per replicate. The experimental treatments were as follows: 1) normal-protein diet (negative control; NC), 2) a reduced- protein diet (20 g kg-1 lower protein) as a control diet (C), 3) C + 2 g kg-1 of PP, 4) C + PP diet supplemented with 250 mg kg-1 VitE, 5) C + PP diet supplemented with 150 mg kg-1 of L-Car and, 6) C + PP diet supplemented with 250 mg kg-1 VitE + 150 mg kg-1 L-Car. Results showed that diets supplemented with PP + VtiE and PP + L-Car showed decreased the number of red blood cells, hemoglobin, hematocrit and heterophil to lymphocyte ratio (P<0.05) at 42 days. Antibody titers against Sheep red blood cells and Newcastle disease virus were significantly increased (P<0.05) at 41 and 27 days by VtiE and L-Car, respectively. While triglycerides and cholesterol were significantly reduced by them (P<0.05). It could be concluded that addition of VtiE and L-Car alone in combination with purslane in low protein diets by create beneficial synergistic effects, it can improve immune system and health in broilers reared at cold stress. | ||
| کلیدواژه‌ها [English] | ||
| Cold stress, Antibody titer, Reduced- protein diet, Hemoglobin | ||
| مراجع | ||
|
Abumweis, S., Barake, R. and Jones, P. (2008). Plant sterols/stanols as cholesterol lowering agents: A meta-analysis of randomized controlled trials. Food & Nutrition Research. 52(1): 1811. Allan, W. H. and Gough, R. E. (1974). A standard haemagglutination inhibition test for Newcastle disease (1), A comparison of macro and micro methods. Veterinary Record. 95(7): 147–149. AOAC .(1994). Association of official analytical chemists.Official Methods of Analysis. Methods of Analysis.16th ed. AOAC,Washington, DC. Arslan, C. (2006). L-Carnitine and its use as a feed additive in poultry feeding a review. Revue de medicine veterinaire. 157(3): 134-142. Asadi, H., Sadeghi, A. A., Eila, N. and Aminafshar, M. (2016). Carcass traits and immune response of broiler chickens fed dietary L-carnitine, coenzyme Q10 and ractopamine. Brazilian Journal of Poultry Science. 18(4): 677-682. Aviagen®: Ross® 308. (2014). Broiler Nutrition Specification, Aviagen Ltd., Midlothian, UK. Baker, F. J. and Silverton, R. E. (1985). Introduction to medical laboratory technology, 6th Editon, Butterworth-Heinemann, Boston, MA. Baghbanzadeh, A. and Decuypere, E. (2008). Ascites syndrome in broilers: physiological and nutritional perspectives. Avian Pathology. 37(2): 117-126. Behrooj, N., Khajali, F. and Hassanpour, H. (2012). Feeding reduced-protein diets to broilers subjected to hypobaric hypoxia is associated with the development of pulmonary hypertension syndrome. British Poultry Science. 53(5): 658-664. El-Sayed, M. I. K. (2011). Effects of Portulaca oleracea L. seeds in treatment of type-2 diabetes mellitus patients as adjunctive and alternative therapy. Journal of Ethnopharmacology. 137(1): 643–651. Farhan, O, M., Mousa, B. H., Alhamdani, A. A. (2019). Effects of dietary L-carnitine to diets with different fat sources on productive performance of broiler. Plant Archives. 19(2): 4409-4414. Geng, A. L., Guo, Y. M. and Yuan, J. (2004). Effects of dietary L-carnitine and coenzyme Q10 supplementation on performance and ascites mortality of broilers. Archives of Animal Nutrition. 58(6): 473-482. Geng, A., Li, B. and Guo, Y. (2007). Effects of dietary L-carnitine and coenzyme Q10 at different supplemental ages on growth performance and some immune response in ascites-susceptible broilers. Archives of Animal Nutrition. 61(1): 50-60. Ghorbani, M. R., Bojarpur, M., Mayahi, M., Fayazi, J., Fatemitabatabaei, R., Tabatabaei, S. and Zulkifli, I. (2014). Effects of purslane extract on performance, immunity responses and cecal microbial population of broiler chickens. Spanish Journal of Agricultural Research. 12(4): 1094-1098. Gross, W.B. (1992). Effect of short-term exposure of chickens to corticosterone on resistance to challenge exposure with Escherichia coli and antibody response to sheep erythrocytes. American Journal of Veterinary Research. 53(3): 291–293. Habibian, M., Sadeghi, G. A. and Karimi, A. (2017). Effects of purslane (Portulaca oleracea L.) powder on growth performance, blood indices, and antioxidant status in broiler chickens with triiodothyronine-induced ascites. Archives Animal Breeding. 60(3): 315-325. Habibian, M., Sadeghi, G. A. and Karimi, A. (2018). Comparative effects of powder, aqueous and methanolic extracts of purslane (Portulaca oleracea L.) on growth performance, antioxidant status, abdominal fat deposition and plasma lipids in broiler chickens. Animal Production Science. 59(1): 89-100. Hassan, M.S.H., Youssef, S.F. and El-bahy, N.M.A. (2011). Effects of l-carnitine and ascorbic acid supplementation on productive, reproductive, physiological and immunological performance of golden montazah laying hens. Egyptian Poultry Science. 31(2): 557-578. Jain, M. C. (1986). Schalm’s Veterinary Haematology, 4th edn., Lea and Febiger, Philadelphia, PA. Kanchana, G. and Jeyanthi, G. P. (2010). The effect of supplementation of diet with vitamin-E and selenium and their combinations on the performance and lipid profiles of layer chickens. International Journal of Pharma and Bio Sciences. 1(1): 1-11. Kazemi, R., Sheikhahmadi, A. and Sadeghi, GH. A, (2018). The effect of purslane hydroextract and zinc on performance, antioxidant capacity and immunity of broiler chickens during summer conditions. Archive Animal Breeding. 61(3): 365-371. Khajali, F. and Sharifi, M. (2018). Fine-tuning low-protein diets through vitamin E supplementation to avoid ascites in broiler chickens. Journal of Agricultural Science and Technology. 20(6): 1127-1135. Leeson, S., Diaz, G. J. and Summers, J. D. (1996). Poultry metabolic disorders and mycotoxins. University Books Publishing, ON, Canada, pp. 44. Lorenzoni, A. G. and Ruiz-Feria, C. A. (2006). Effects of vitamin E and L-arginine on cardiopulmonary function and ascites parameters in broiler chickens reared under subnormal temperatures. Poultry Science. 85(12): 2241-2250. Lucas, A. M. and Jamroz, C. (1961). Atlas of Avian Hematology. Agriculture Monograph 25. USDept. Agric., Washington, DC. Moriguchi, S., MiwaI, H., Okamura, M., Maekawa, K., Kishino, Y. and Maeda, K. (1993) Vitamin E is an important factor in T cell differentiation in thymus of F344 rats. Journal of Nutrition Science and Vitaminology. 39(5): 451–463. Nemati, M. H., Shahir, M. H., Harakinezhad, M. T. and Lotfalhian, H. (2017). Cold-induced ascites in broilers: effects of vitamin C and coenzyme Q10. Brazilian Journal of Poultry Scienc. 19(3): 537-544. Okafor, I. A., Ayalokunrin, M. B. and Orachu, L. A. (2014). A review on Portulaca oleracea (purslane) plant – its nature and biomedical benefits. International Journal of Biomedical Research. 5(2): 75–80. Olson, D.W., Sunde, M.L. and Bird, H.R. (1972). The effect of temperature on metabolizable energy determination and utilization by the growing chick. Poultry Science. 51(6): 1915-1922. Ozkan, S., Basmacioglu Malayoglu, H., Yalcin, S., Karadas, F., Kocturk, S., Cabuk, M., Oktay, G., Ozdemir, S., Ozdemir, E. and Ergul, M. (2007). Dietary vitamin E (a-tocopherol acetate) and selenium supplementation from different sources: performance, ascites-related variables and antioxidant status in broilers reared at low and optimum temperatures. British Poultry Science. 48(5): 580-593. Pourmorad, F., Hosseinimehr, S. J. and Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology. 5(11): 1142-1145. Rehman, Z., Chand, N. and Khan, R. U. (2017a). The effect of vitamin E, L-carnitine, and ginger on production traits, immune response, and antioxidant status in two broiler strains exposed to chronic heat stress. Environ. Environmental Science and Pollution Research. 24: 26851-26857. Rehman, Z., Naz, S., Khan, R. U. and Tahir, M. ( 2017b). An update on potential applications of L-carnitine in poultry. World's Poultry Science Association. 73(4): 1-8. Saffaryazdi, A., Ganjeali, A., Farhoosh, R. and Cheniany, M. (2020). Variation in phenolic compounds, α- linolenic acid and linoleic acid contents and antioxidant activity of purslane (Portulaca oleracea L.) during phenological growth stages. Physiology and Molecular Biology of Plants. 26(7): 1519 – 1529. SAS. (2003). SAS Stat User’s Guide Release 9.1. SAS Inc. Cary. NC. Sharifi, M. R., Khajali, F., Hassanpour, H., Pour-Reza, J. and Pirany, N. (2015a). L-arginine supplementation of reduced-protein diets improves pulmonary hypertensive response in broiler chickens reared at high altitude. British Poultry Science. 56(4): 470-476. Sharifi, M. R., Khajali, F., Hassanpour, H., Pour-Reza, J. and Pirany. N. (2015b). Supplemental L-arginine modulates developmental pulmonary hypertension in broiler chickens fed reduced-protein diets and reared at high altitude. Poultry Science Journal. 3(1): 47-58. Sharifi, M. R., Khajali, F. and Hassanpour, H. (2016). Antioxidant supplementation of low-protein diets reduced susceptibility to pulmonary hypertension in broiler chickens raised at high altitude. Journal of Animal Physiology and Animal Nutrition. 100(1): 69-76. Sigolo, S., Khazaei, R., Seidavi, A., Ayasan, T., Gallo, A. and Prandini, A. (2019). Effects of supra nutritional levels of vitamin E and vitamin C on growth performance and blood parameters of Japanese quails. Italian Journal of Animal Science. 18(1): 140-146. Wang, Y., Guo, Y., Ning, D., Peng, Y., Cai, H., Tan, J., Yang, Y. and Liu, D. (2012). Changes of hepatic biochemical parameters andproteomics in broilers with cold-induced ascites. Journal of Animal Science and Biotechnology. 41(3): 1-9. Wang, Y. W., Ning, D., Peng, Y. Z. and Guo, Y. M. (2013). Effects of dietary L-carnitine supplementation on growth performance, organ weight, biochemical parameters and ascites susceptibility in broilers reared under low-temperature environment. Asian-Australas Journal Animal Scince. 26(2): 233-240. Wideman, R. F. (2000). Cardio-pulmonary hemodynamics and ascites in broiler chickens. Poultry and Avian Biology Reviews. 11(1): 24–43. Wideman, R. F., Rhoads, D. D., Erf, G. F. and Anthony, N. B. (2013). Pulmonary arterial hypertension (ascites syndrome) in broilers: a review. Poultry Science. 92(1):64-83. Yersin, A.G., Huff, W.E., Kubena, L.F., Elissalde, M.H., Harvey, R.B., Witzel, D.A. and Giroir, L.E. (1992). Changes in hematological, blood gas, and serum biochemical variables in broilers during exposure to simulated high altitude. Avian Diseases. 36(2): 189-196. Zhang, Y., Ma, Q., Bai, X., Zhao, L., Wang, Q. and Ji, C. (2010). Effects of dietary acetyl- L-carnitine on meat quality and lipid metabolism in Arbor Acres Broilers. Asian-Australasian Journal of Animal Science. 23(12): 1639-1644. | ||
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