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Comparison of Lowenstein Jensen Media and Ogawa Media Usage for Viability Test of BCG Vaccine Pasteur P11732 and Russian (Moscow) – 384 sub-strains | ||
| Archives of Razi Institute | ||
| مقاله 26، دوره 79، شماره 1، فروردین و اردیبهشت 2024، صفحه 240-245 اصل مقاله (293.26 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.1.240 | ||
| نویسنده | ||
| Muhammad Erdiansyah* | ||
| National Quality Control Laboratory of Drug and Food (NQCLDF), Indonesian FDA, Central Jakarta, Indonesia | ||
| چکیده | ||
| BCG vaccines on the market have employed a Mycobacterium bovis sub-strains derived from the initial strain. To date, there has been no recommendation regarding the sub-strains with the highest effectiveness when administered to humans. Because it remains the standard for Tuberculosis treatment, the BCG vaccine's quality must be verified. One of the parameters for BCG vaccine quality control is the test for viability. The culture method has become the gold standard for viability testing with various testing media. This study aims to evaluate the performance of Lowenstein Jensen and Ogawa media for the viability test of Pasteur 1173P2 and Russian (Moscow) – 384 sub-strains of M. bovis in the BCG vaccine. The number of culturable particles of each sub-strain in the BCG vaccine was estimated and statistically evaluated using the t-test. The Colonies of the Pasteur 1173P2 has characteristics; tended to clump on both mediums with tiny, rough, and pale yellow/cream colors. While the colony character of the Russian (Moscow) – 384 generally has similar feature but did not cluster and had a smooth texture. In terms of growth rate, Lowenstein Jensen and Ogawa media performed similarly for Pasteur 1173P2 and Russian (Moscow) – 384 sub-strains. Maximum growth is reached by week 5. The culturable particles of Pasteur P1173P2 sub-strains did not differ between mediums. Whereas the growth of the Russian (Moscow) - 384 sub-strains was statistically better on Ogawa media. The results of this study reveal that the performance of the media used for determining the number of culturable particles is based on the sub-strains of M. bovis present in the BCG vaccine. | ||
| کلیدواژهها | ||
| BCG Vaccine؛ Culture Media؛ Microbial Viability؛ Vaccine potency؛ Mycobacterium bovis | ||
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References 1. Ritz N, Dutta B, Donath S, Casalaz D, Connell TG, Tebruegge M, Robins-Browne R, Hanekom WA, Britton WJ, Curtis N. The influence of bacille Calmette-Guérin vaccine strain on the immune response against tuberculosis: A randomized trial. Am J Respir Crit Care Med. 2012; 185:213–222. doi: 10.1164/rccm.201104- 0714OC. PMID: 22071384. 2. Pascoe J, Hendon-Dunn CL, Birch CPD, Williams GA, Chambers MA, Bacon J. Optimisation of mycobacterium bovis BCG fermentation and storage survival. Pharmaceutics. 2020; 12:1–14. doi: 10.3390/pharmaceutics12090900. 3. Palange P, Narang R, Kandi V. Evaluation of Culture Media for Isolation of Mycobacterium Species from Human Clinical Specimens. Cureus. 2016; 8:e757. doi: 10.7759/cureus.757. 4. Naveen G, Peerapur B v. Comparison of the Lowenstein-Jensen medium, the middlebrook 7H10 medium and MB/BACT for the isolation of Mycobacterium tuberculosis (MTB) from clinical specimens. Journal of Clinical and Diagnostic Research. 2012; 6:1704–1709. doi: 10.7860/JCDR/2012/4603.2635. PMID: 23373033. 5. Costa RR da, Silva SF da, Fochat RC, Macedo RL, Pereira TV, Silva MR, Pinto CPG, Leite ICG. Comparison between Ogawa-Kudoh and modified Petroff techniques for mycobacteria cultivation in the diagnosis of pulmonary tuberculosis. Einstein (Sao Paulo). 2018; 16:eAO4214. doi: 10.1590/S1679-45082018AO4214. PMID: 29898027. 6. Franco-Sotomayor G, Rivera-Olivero IA, LeonBenitez M, Uruchima-Campoverde SE, Cardenas-Franco G, Perdomo-Castro ME, Cardenas-Franco CS, OrtegaVivanco J, Abad-Ruiz AS, de Waard JH, et al. Fast, Simple, and Cheap: the Kudoh-Ogawa Swab Method as an Alternative to the Petroff-Lowenstein-Jensen Method for Culturing of Mycobacterium tuberculosis. J Clin Microbiol. 2020; 58:e01424-19. doi: 10.1128/JCM.01424-19. 7. Jensen KA. Bulletin of the International Union against Tuberculosis. 1954; 24:78–112. PMID: 1756295. 8. Obayashi Y. Dried BCG Vaccine [Internet]. Geneva: World Health Organization; 1955. Available from: https://apps.who.int/iris/handle/10665/41663. 9. World Health Organization. In Vitro Assays of BCG Products [Internet]. 1977. Available from: https://apps.who.int/iris/handle/10665/99372. 10. Tanoue S, Mitarai S, Shishido H. Comparative study on the use of solid media: Löwenstein-Jensen and Ogawa in the determination of anti-tuberculosis drug susceptibility. Tuberculosis. 2002; 82:63–67. doi: 10.1054/tube.2002.0323. | ||
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