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ارزیابی خلوص ژنتیکی بذر ارقام پنبه (Gossypium hirsutum L.)با استفاده از صفات مورفولوژیک و نشانگرهای ریزماهواره | ||
| علوم و فناوری بذر ایران | ||
| مقاله 2، دوره 12، شماره 3 - شماره پیاپی 31، مهر 1402، صفحه 13-24 اصل مقاله (1.33 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2023.361051.1466 | ||
| نویسندگان | ||
| مریم نجفیان فخرائی1؛ قاسم توحیدلو* 2؛ آیدین حمیدی3؛ فرزاد پاکنژاد2؛ مهدی رضائی4 | ||
| 1دانشگاه آزاد واحد کرج | ||
| 2دانشگاه آزاد اسلامی واحد کرج | ||
| 3سازمان تحقیقات، آموزش و ترویج کشاورزی-مؤسسه تحقیقات ثبت و گواهی بذر نهال | ||
| 4موسسه تحقیقات ثبت و گواهی بذر و نهال | ||
| چکیده | ||
| به منظور ارزیابی خلوص ژنتیکی و اصالت سه رقم تجاری رایج پنبه (ورامین، خرداد و بختگان) با استفاده از صفات مورفولوژیک و نشانگرهای ریزماهواره، بذور این ارقام به همراه لاین های خالص در مزرعه در قالب یک طرح دوسالهی بلوک کامل تصادفی با چهار تکرار با دو روش آزمون رشد مزرعهای (GOT) و نشانگرهای ریزماهواره آزمایش شد. نتایج آزمونGOT نشان داد که نمونه های خالص ارقام در ۷ صفت مورفولوژیک با نمونههای خارج از تیپ و غیرخالص تفاوت داشته و میتوان از این صفات که تحت تاثیر محیط نمیباشند، به عنوان کلید شناسایی رقم مورد نظر استفاده کرد. در آزمایش ملکولی، از ۷ نشانگر ، ۳ جفت به دلیل قدرت تفکیک و تعداد آلل موثر (DPL431,DPL0513,CIR246) انتخاب شدند. با توجه به مشابه بودن نتایج دو روش GOT و نشانگرهای ریزماهواره و با توجه به گران بودن روش ملکولی، میتوان از این روش به عنوان روش سریع، موردی و تکمیلی برای تعیین درصد خلوص ژنتیکی ارقام استفاده کرد. | ||
| کلیدواژهها | ||
| واژه های کلیدی: پنبه؛ آزمون خلوص ژنتیکی؛ نشانگر ریز ماهواره | ||
| عنوان مقاله [English] | ||
| Assessment of genetic purity of cotton cultivars (Gossypium hirsutum L.) using morphological characteristics and microsatellite marker (SSR) | ||
| نویسندگان [English] | ||
| maryam najafian1؛ Ghasem tohidloo2؛ A. Hamidi3؛ farzad paknejad2؛ mehdi rezaei4 | ||
| 1Islamic Azad University, Karaj branch | ||
| 2Islamic Azad University, Karaj branch | ||
| 3Agriculture Research, Education and Extension Organization-Seed and Plant Certification and Registration Institute | ||
| 4Seed and Plant Certification and Registration Institute (SPCRI), Karaj | ||
| چکیده [English] | ||
| In order to evaluate the genetic purity and originality of three common commercial cotton cultivars (Varamin, Khordad and Bakhtegan) using morphological characteristics and microsatellite markers, the seeds of these cultivars along with pure lines in the field in a two-year randomized complete block design with four replications two methods of field growth test (GOT) and microsatellite markers were tested. The results of the GOT test showed that the pure samples of the cultivars were different from the off-type and non-pure samples in 7 morphological characteristics, and these traits, which are not influenced by the environment, can be used as a key to identify the desired cultivar. In the molecular test, out of 7 markers, 3 pairs were selected due to their resolution and the number of effective alleles (DPL431, DPL0513, and CIR246). Due to the similarity of the results of GOT methods and microsatellite markers and due to the high cost of the molecular method, this method can be used as a quick, case-by-case and complementary method to determine the genetic purity percentage of cultivars. | ||
| کلیدواژهها [English] | ||
| Keywords: Cotton, . Genetic purity .test, Microsatellite marker | ||
| مراجع | ||
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Agrawal, P.K.1992. Cultivar purity. Pp170-178. In: Seed Sci. Technol., South Asian Pub. PVT. LTD. New Delhi, India. Agrawal, R.L. 1997. Determination of genuiness of varieties, In: Seed technol. (2 nd. Ed.), pp: 499-513. Oxford & IBH Pub. Co. Pvt. Ltd., India. Agrawal, P.K. 2002. Cultivar purity test, In: Principles of seed technol, pp: 96-104. Indian Council of Agricultural Research, New Delhi, India. ELÇİ, E., Y. AKIŞCAN, and B. AKGÖL. 2014. "Genetic diversity of Turkish commercial cotton varieties revealed by molecular markers and fiber quality traits." Turk. J. Bot. 38(6): 1274-1286. Doi: 10.3906/bot-1405-78 Hamidi, A., J. Rezazadeh, V. Askari, F. Hassanpour, F. Moghimian, M. Jazaeri Noosh Abadi, and E. Baniani. 2011. Identification and registration of Cotton (Gossypium hirsutum L.) cultivars by using morphological charactristics. Research project final report. Ministry of Jihad–e Agriculture Agricultural Research Education and Extension Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI), Registration No.: 89/1761. Doi: 10.22092/IJCR.2017.115571. (In Persian, with English Abstract) Hamidi, A., O. Alishah, M.R. Rahemi, A. Mohajer Abbasi, Y. Jafari, J. Hoseinpoor, K. Ghasemi Bezdi, M. Jazaeri Noosh Abadi, and M. Najafian. 2022. Evaluation of some quantitative and qualitative characteristics of six newly introduced genotypes of cotton (Gossypium hirsutum L.). Iranian J. Cotton Res. 9(2): 179-201. Doi: 10.22092/ijcr.2022.359154.1184. (In Persian, with English Abstract) Jeevan Kumar, S.P., C. Susmita, D. K. Agarwal, G. Pal., A. Kumar Rai, and J. Simal-Gandara. 2021. Assessment of genetic purity in rice using polymorphic SSR markers and its economic analysis with grow-out-test. Food Anal. Methods. 14(5): 856-864. Doi: 10.1007/s12161-020-01927-9 Kumar, P., S. Nimbal, N. Budhlakoti, V. Singh, and R.S. Sangwan. 2022. Genetic diversity and population structure analysis for morphological traits in upland cotton (Gossypium hirsutum L.). J. Appl. Genet. 63(1):.87-101. Doi: 10.1007/s13353-021-00667-8 Li, YC., A.B. Korol, T. Fahima, A. Beiles, E. Nevo. 2002. Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review. Mol. Ecol. 11(12):2453–2465. Doi: 10.1046/j.1365-294x.2002.01643.x Ministry of Jihad-e-Agriculture, 2022. Crops area, production and yield report in 2020-21 crop year. Information and Communication Technology Center of Ministry of Jihad-e-Agriculture, Tehran, Iran. (In Persian) Mozafari, J., S.Y. Sadeghian, S. Mobasser, H. Khademi, and S.A. Mohammadi. 2010. Principles of plant variety protection. Ministry of Jihad-e-Agriculture Agricultural Research Education and Extensions Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI), Tehran, Iran. (In Persian) Noormohammadi, Z., Y. Hasheminejad-Ahangarani Farahani, M. Sheidai, S. Ghasemzadeh-Baraki, and O. Alishah. 2013. Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers. Genet. Mol. Res. 12(1): 256-269. Doi: 10.4238/2013.January.30.12 Rana M. K., S. Singh, K.V. Bhat. 2007. RAPD, STMS and ISSR markers for genetic diversity and hybrid seed purity testing in cotton. Seed Sci. Technol. 35(3):709–721. Doi: 10.15258/sst.2007.35.3.17 Saeed, A., and E. Elçi., 2018. "Microsatellite-based characterization of cotton genotypes for verticillium wilt and fiber quality traits." Turkish J. Biochem. 43(3): 277-288. Doi: 10.1515/tjb-2017-0169 Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen, and R.W.L. Allard. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. 81(24): 8014-8018. Saleem MA, M.W. Amjid, M.Q. Ahmad, H. Riaz, S.F. Arshad, Z.U. Zia. 2020. EST-SSR based analysis revealed narrow genetic base of in-use cotton varieties of pakistan. Pakistan J. Bot. 52(5): 1667-1672. Doi: 10.30848/PJB2020-5(32) Santosh, H.B., A. Bargat, V. Santhy, K.P. Raghavendra, K.R. Kranthi, and V.N. Waghmare. 2022. Microsatellite marker based DNA fingerprinting of cotton (Gossypium spp.) hybrids and their parents. Electron. J. Plant Breed. 13(3): 780-789. Doi: 10.37992/2022.1303.136 Silvanacristae, S.M., J.F.M. Moistsai, M.A. Valls, A. Marcos, and C.R. Lopes. 2005. Genetic characterization of Brazilian annual Arachis species from selections Arachis and heteranthae using RAPD markers. Genet. Resour. Crop. Ev. 52(8): 1079-1086. Doi: 10.1007/s10722-004-6098-9 Tatineni, V., R. G. Cantrell, and D. D. Davis, 1996. Genetic diversity in elite cotton germplasm determined by morphological characteristics and RAPDs. Crop Sci. 36(1):186-192. Doi: 10.2135/cropsci1996.0011183X003600010033x Tyagi P, M.A. Gore, D.T. Bowman, B.T. Campbell, J.A. Udall, and V. Kuraparthy. 2014. Genetic diversity and population structure in the US Upland cotton (Gossypium hirsutum L.). Theor. Appl. Genet. 127(2): 283-295. Doi: 10.1007/s00122-013-2217-3 UPOV (International Union for the Protection of new Varieties of Plants). 2018. Guidelines for the conduct of tests for Distinctness, Uniformity and Stability in Cotton. TG/88/7. Geneva, Switzerland. Zhang YC, M. Kuang, W.H. Yang, H.X. Xu, D.Y. Zhou, Y.Q. Wang, X.A. Feng, C. Su, and F. Wang. 2013. Construction of a primary DNA fingerprint database for cotton cultivars. Genet. Mol. Res. 12(2):1897-1906. Doi: 10.4238/2013.January.30.3 | ||
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