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Wanna, Devotha, Mzula, Alexanda, Mwega, Elisa. (1402). Antibacterial Potential and Safety Level of Euphorbia tirucalli and Vernonia glabra Commonly used by Residents in Iringa, Tanzania. سامانه مدیریت نشریات علمی, 12(3), 283-292. doi: 10.22034/jmpb.2023.361209.1525
Devotha Yahaya Wanna; Alexanda Mzula; Elisa Daniel Mwega. "Antibacterial Potential and Safety Level of Euphorbia tirucalli and Vernonia glabra Commonly used by Residents in Iringa, Tanzania". سامانه مدیریت نشریات علمی, 12, 3, 1402, 283-292. doi: 10.22034/jmpb.2023.361209.1525
Wanna, Devotha, Mzula, Alexanda, Mwega, Elisa. (1402). 'Antibacterial Potential and Safety Level of Euphorbia tirucalli and Vernonia glabra Commonly used by Residents in Iringa, Tanzania', سامانه مدیریت نشریات علمی, 12(3), pp. 283-292. doi: 10.22034/jmpb.2023.361209.1525
Wanna, Devotha, Mzula, Alexanda, Mwega, Elisa. Antibacterial Potential and Safety Level of Euphorbia tirucalli and Vernonia glabra Commonly used by Residents in Iringa, Tanzania. سامانه مدیریت نشریات علمی, 1402; 12(3): 283-292. doi: 10.22034/jmpb.2023.361209.1525

Antibacterial Potential and Safety Level of Euphorbia tirucalli and Vernonia glabra Commonly used by Residents in Iringa, Tanzania

Journal of Medicinal plants and By-products
دوره 12، شماره 3، آذر 2023، صفحه 283-292    اصل مقاله (999.6 K)
نوع مقاله: Research Article
شناسه دیجیتال (DOI): 10.22034/jmpb.2023.361209.1525
نویسندگان
Devotha Yahaya Wanna* 1، 2؛ Alexanda Mzula1؛ Elisa Daniel Mwega1
1Department of Veterinary Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture. P. O Box 3019 Morogoro, Tanzania
2Department of Biological Sciences, Faculty of Sciences, Mkwawa University College of Education. Iringa, Tanzania
چکیده
This study was conducted to evaluate the antibacterial potential of Euphorbia tirucalli L. and Vernonia glabra (Steetz) Vatke and their safety level.  The plants were obtained from Kiwere ward in the Iringa district, prepared and extracted in aqueous and ethanol solvents. The E. tirucalli and V. glabra plant extracts were studied for their antimicrobial activities against E. coli isolates and E. coli ATCC 25922 using the agar well diffusion method. Minimum inhibitory and bactericidal concentrations (MIC and MBC) were determined. Among the tested plant extracts, the ethanol extracts of V. glabra showed the highest activity against E. coli isolates and E. coli 25922 with a mean inhibition zone of 17.909±0.3297 and 22.5 ± 0.500 respectively. Furthermore, on MIC and MBC, the ethanol extracts of V. glabra had a better activity with the values ranging from 1.25-2.5 mg/ml and 2.5-5 mg/ml respectively compared to other plant extracts. Phytochemical screening revealed the extract’s presence of alkaloids, flavonoids, tannins, saponins, triterpenes and steroids. The brine shrimp lethality test showed that the aqueous extract of E. tirucalli was non-toxic with LC50 of 1007 µg/mL, while ethanol extract of E. tirucalli, aqueous extract of V. glabra and ethanol extract of V. glabra were less toxic with LC50 values of 589, 507 and 658 µg/mL respectively.  These results indicate that the plant extracts have bioactive constituents that could be accounted for their pharmacological properties. Furthermore, these results proved the claim of indigenous people in the study area on the effectiveness and safety of the plants and supported the use of these plant species in traditional medicine. 
کلیدواژه‌ها
Antibacterial Activity؛ Euphorbia Tirucalli؛ Vernonia Glabra؛ Escherichia Coli؛ Phytochemical Screening؛ Brine Shrimp Lethality Test
مراجع
  1. Sophy R.A., Fleming A.T., Ronald B., Shankar K., Vidhya R., Rajagopalan V., Sheeba A., Durgalakishmi R. Antimicrobial activity of extracts of Adenanthera pavonina and Mussaenda philippica against isolated bacteria and fungi. Inter J Life Sci & Pharmaceu Res. 2015; 5:21-26.
  2. Petrovska B.B. Historical review of medicinal plants’ usage. Pharmacognosy Reviews. 2012; 6: 1–5.
  3. Jain C., Khatana S., Vijayvergia R. Bioactivity of secondary metabolites of various plants: A Review Article. Inter J Pharmaceu Sci & Res. 2019;10: 494.
  4. Sharma N., Samarakoon K.W., Gyawali R., Park Y.H., Lee S.J., Oh S.J. Evaluation of the antioxidant, anti-inflammatory, and anticancer activities of Euphorbia hirta ethanolic extract. Molecules. 2014; 19:14567–81.
  5. Parveen A., Parveen B., Parveen R., Ahmed S. Challenges and guidelines for clinical trial of herbal drugs. J Pharmaceu Bioallied Sci. 2015; 7:329–33.
  6. Mada S.B., Garba A., Mohammed H.A., Muhammad A., Olagunju A., Muhammad A.B. Antimicrobial activity and phytochemical screening of aqueous and ethanol extracts of Momordica charantia L. leaves. J Med Plants Res. 2013; 7:579–86.
  7. Pavithra P.S., Janani V.S., Charumathi KH., Indumathy R., Potala S., Verma R.S. Antibacterial activity of plants used in Indian herbal medicine. Inter J Green Pharmacy. 2010; 4:22–8.
  8. Nascimento G.G.F., Locatelli J., Freitas P.C., Silva G.L. Antibacterial activity of plant extracts and Phytochemicals on antibiotic-resistant bacteria. Brazilian J Microbio. 2000; 31:247-256.
  9. Rocha M.F., de Aguiar F.L.N., Brilhante R.S.N. Moringa oleifera and Vernonia sp. extracts. Ciencia Rular. 2011; 41:1807–13.
  10. Thomas J., Sivadasan M.., Al-Ansari AM., Alfarhan A., El-Sheikh M., Basahi M. Alatar AA. New generic and species records for the flora of Saudi Arabia. Saudi J Biol Sci. 2014; 21:457–64.
  11. Van Damme P.L.J. Euphorbia tirucalli for High Biomass Production. Coupure Links, 2001.
  12. Upadhyay B., Singh K.P., Kumar A. Ethno-Medicinal, Phytochemical and Antimicrobial Studies of Euphorbia tirucalli L. J Phytology. 2010; 2010:65–77.
  13. de Araújo K.M., de Lima A., Silva J.D.N., Rodrigues L.L., Amorim A.G.N., Quelemes P., et al. Identification of phenolic compounds and evaluation of antioxidant and antimicrobial properties of Euphorbia tirucalli L. Antioxidants. 2014; 3:159–75.
  14. Santana S.S., Gennari-Cardoso M.L., Carvalho F.C., Roque-Barreira M.C., Santiago A.D.S., Alvim F.C., Pirovan C.P. Eutirucallin, a RIP-2 type lectin from the latex of Euphorbia tirucalli L. presents proinflammatory properties. PLoS One. 2014;9.
  15. de Souza L.S., Puziol L.C., Tosta C.L., Bitternourt M.L.F. Analytical methods to access the chemical composition of an E. tirucalli anticancer latex from traditional Brazilian medicine. J Ethnopharmacol. 2019; 237:255–65.
  16. Gupta N., Vishnoi G., Wal A., Wal P., Pradesh U. Research and Reviews: J Pharmacognosy and Phytochemistry- Medicinal Value of Euphorbia tirucalli: A Review. 2013;1.
  17. Ngonda F., Magombo Z., Mpeketula P., Mwatseteza J. Evaluation of malawian Vernonia glabra (Steetz)Vatke leaf and Securidaca longepedunculata (Fresen) root extracts for antimicrobial activities. J Appl Pharm Sci. 2012; 2:26–33.
  18. Hattori EKO, Nakajimana J.N. A familia Asteraceae na Estacao. Brasil-Rodriquesia. 2008; 59:687–749.
  19. Teles A.M., Bautista H.P. Asteraceae no Parque Metropolitano de Pituaçu, Salvador, Bahia, Brasil. Lundiana. 2006; 7:87–96.
  20. Kitonde CK., Fidahusein D.S., Lukhoba C.W., Jumba M.M. Antimicrobial activity and phytochemical study of Vernonia glabra (Steetz) Oliv. & Hiern. in Kenya. AJTCAM / African Networks on Ethnomed. 2012; 10:149–57.
  21. Toyang J.N., Verpoorte R. A review of the medicinal potentials of plants of the genus Vernonia (Asteraceae). J Ethnopharmacol. 2013; 146:681–723.
  22. Nondo R.S.O., Moshi M.J., Erasto P., Zofou D., Njouendou A.J., Wanji S., et al. Evaluation of the cytotoxic activity of extracts from medicinal plants used for the treatment of malaria in Kagera and Lindi regions, Tanzania. J Appl Pharm Sci. 2015; 5:7–12.
  23. Ruffo CK. A Survey of medicinal plants in Tabora region, Tanzania. 1991.
  24. Nhung N.T., Cuong N., Thwaites G., Carrique-Mas J. Antimicrobial usage and antimicrobial resistance in animal production in Southeast Asia: A review. Antibiotics. MDPI AG; 2016;5.
  25. Ramos S., Silva V., Dapkevicius M.D., Caniça M., Tejedor-Junco M.T., Igrejas G., Poeta P. Escherichia coli as commensal and pathogenic bacteria among food-producing animals: Health implications of extended spectrum β-lactamase (ESBL) production. Animals. 2020; 10:2239.
  26. Augusto M., Medeiros N., Nunes De Oliveira D.C., Dos D., Rodrigues P., Coradi De Freitas DR. Prevalence and antimicrobial resistance of Salmonella in chicken carcasses at retail in 15 Brazilian cities. Rev Panam Salud Publica. 2011;30.
  27. Nolan L.CK., John Barnes H., Vaillancourt J.P., Abdul-Aziz T., Logue C.M. Diseases of Poultry. 2013.
  28. Subramanya SH., Bairy I., Metok Y., Baral B.P., Gautam D., Nayak N. Detection and characterization of ESBL-producing Enterobacteriaceae from the gut of subsistence farmers, their livestock, and the surrounding environment in rural Nepal. Sci Rep. 2021;11.
  29. Kheiri R., Akhtari L. Antimicrobial resistance and integron gene cassette arrays in commensal Escherichia coli from human and animal sources in IRI. Gut Pathog. 2016;8.
  30. Valgas C., Machado De Souza S., Elza, Smânia F.A., Smânia A. Screening methods to determine antibacterial activity of natural products. Brazilian J Microbio. 2007; 38:369–80.
  31. Mishra S.K., Tripathi S., Shukla A., Oh SH., Kim H.M. Andrographolide and analogues in Cancer prevention. Frontiers in Bioscience-Elite. 2015; 7:293–304.
  32. Sahira Banu K., Cathrine L. General Techniques Involved in Phytochemical Analysis Related Papers Extraction, Isolation and Characterization of Bioactive Compounds from Plant. Inter J Adv Res Chem Sci (IJARCS). 2015; 2:25–32.
  33. Doughari J.H., Ndakidemi P.A., Human I.S., Benade S. Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dantata. Ethnopharmacol J. 2012;141 :1041–50.
  34. Mamta S., Jyoti S. Phytochemical screening of Acorus calamus and Lantana camara. Inter J Pharmacy. 2012;3 :324–6.
  35. Usman H. Usman. Qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of Ficus thonningii (MORACEAE). Afr Trad J. CAM. 2009; 6.
  36. Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobson L.B., Nichols D.E.J., McLaughlin J.L. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982;45 :31–4.
  37. Clarkson C., Muharaj V.J., Crouch N.R., Grace O.M., Pillay P., Matsabisa M.G., et al. In Vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa. J Ethnopharmacol. 2004; 92:177–91.
  38. Valeeva L.R., Dague A.L., Hall M.H., Tikhonova A.E., Sharipova M.R., Valentovic M.A., et al. Antimicrobial Activities of Secondary Metabolites from Model Mosses. Antibiotics. 2022;11.
  39. Bhalodia N.R., Nariya P.B., Shukla V.J. Antibacterial and antifungal activity from flower extracts of Cassia Fistula L.: An ethnomedicinal plant. Int J Pharmtech Res. 2011; 3:160–8.
  40. Kane S.R., Bhandari S. Invitro Phytochemical Investigation, Characterization & Antimicrobial Study of Latex of Euphorbia tirucalli Linn. Inter J ChemTech Res 2013; 5:2344-2346.
  41. Antonio C.N.S., Elnatan B de S., Raquel O dos S.F. A review on antimicrobial potential of species of the genus Vernonia (Asteraceae). J Med Plants Res. 2015; 9:838–50.
  42. Sultan S., Kimaro C., Amri E. Antifungal Activity and Phytochemical Screening of Different Solvent Extracts of Euphorbia tirucalli Linn. J Adv Biol Biotechnol. 2016; 7:1–9.
  43. Welde M., Nair S.P. Preliminary phytochemical screening and in vitro antibacterial activity of different solvent extracts of leaf and stem of Euphorbia ampliphylla pax (FAMILY-EUPHORBIACEAE). World J Pharmaceu Res. 2020; 9:1840-1849.
  44. Donadio G., Mensitieri F., Santoro, V., Parisi V., Bellone M. L., De Tommasi N., Dal Piaz F. Interactions with microbial proteins driving the antibacterial activity of flavonoids. Pharmaceutics 2021;13: 660.
  45. Tong Z., He W., Fan X., Guo A. Biological Function of Plant Tannin and Its Application in Animal Health. Vol. 8, Frontiers in Veterinary Science. Frontiers Media S.A.; 2022.
  46. Bhattacharya A. High-Temperature Stress and Metabolism of Secondary Metabolites in Plants. Effect of High Temperature on Crop Productivity and Metabolism of Macro Molecules. 2019;391–484.
  47. Chen D., Hasnain A., Atif H.N.S., Mubeen A., Rizwan M., Adress M., Iqbal S., et al. Promising Secondary Metabolites to Confer Resistance Against Environmental Stresses in Crop Plants: Current Scenario and Future Perspectives. 2022; 13:1-26.
  48. Mohamad S. The Antibacterial Properties of Euphorbia tirucalli Stem Extracts against Dental Caries-Related Bacteria. Med Health. 2017; 12:34–41.
  49. Mwakalukwa R., Maregesi S.M. Ethnopharmacological Study on Medicinal Plants Used to Treat Infectious Diseases in the Rungwe District, Tanzania. Inter J Med Plants & Natural Products (IJMPNP). 2015; 1:15–22.
  50. Qwarse M., Mihale M.J., Mugoyela V., Sempombe J., Henry L., Sunghwa F. Ethnobotanical Survey of Medicinal and Pesticidal Plants used by Agro-pastoral Communities in Mbulu District, Tanzania. Sci & Technol J. 2018; 1:22-35.
  51. Parekesh J., Jadeja D., Chanda S. Efficacy of Aqueous and Methanol Extracts of Some Medicinal Plants for Potential Antimicrobial Activity. Turk J Biol. 2005; 29:203-210.
  52. Lavilla J.r. C.A., Uy M.M., Ohta S. Cytotoxic Long-chain Alkene and Terpene Isolated from the Methanol Extract of the Air-dried Leaves of Pipturus arborescens C.B. Rob. Multidiscipl Studies J. 2014;3.
  53. Mackeen M., Khan M.N., Samadi Z., Lajis N.H. Brine Shrimp Toxicity of Fractionated Extracts of Malaysian Medicinal Plant. Natural Product Sci. 2000; 6:131-134.
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