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Malik, Y., Ackakzai, W.M., Mustafa, S., Saddozai, S., Akbar, A.. (1402). Accumulation of heavy metals and detection of resistant-associated genes in Pseudomonas aeruginosa in an edible catfish (Wallago attu) from Pat Feeder Canal, Pakistan. سامانه مدیریت نشریات علمی, 22(3), 602-614. doi: 10.22092/ijfs.2023.129461
Y. Malik; W.M. Ackakzai; S. Mustafa; S. Saddozai; A. Akbar. "Accumulation of heavy metals and detection of resistant-associated genes in Pseudomonas aeruginosa in an edible catfish (Wallago attu) from Pat Feeder Canal, Pakistan". سامانه مدیریت نشریات علمی, 22, 3, 1402, 602-614. doi: 10.22092/ijfs.2023.129461
Malik, Y., Ackakzai, W.M., Mustafa, S., Saddozai, S., Akbar, A.. (1402). 'Accumulation of heavy metals and detection of resistant-associated genes in Pseudomonas aeruginosa in an edible catfish (Wallago attu) from Pat Feeder Canal, Pakistan', سامانه مدیریت نشریات علمی, 22(3), pp. 602-614. doi: 10.22092/ijfs.2023.129461
Malik, Y., Ackakzai, W.M., Mustafa, S., Saddozai, S., Akbar, A.. Accumulation of heavy metals and detection of resistant-associated genes in Pseudomonas aeruginosa in an edible catfish (Wallago attu) from Pat Feeder Canal, Pakistan. سامانه مدیریت نشریات علمی, 1402; 22(3): 602-614. doi: 10.22092/ijfs.2023.129461

Accumulation of heavy metals and detection of resistant-associated genes in Pseudomonas aeruginosa in an edible catfish (Wallago attu) from Pat Feeder Canal, Pakistan

Iranian Journal of Fisheries Sciences
دوره 22، شماره 3، مرداد 2023، صفحه 602-614    اصل مقاله (683.91 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2023.129461
نویسندگان
Y. Malik* 1؛ W.M. Ackakzai1؛ S. Mustafa2؛ S. Saddozai3؛ A. Akbar4
1Department of Zoology, University of Balochistan Quetta, 87300, Pakistan
2Department of Chemistry, University of Balochistan Quetta, 87300, Pakistan
3Department of Zoology, SBK Women’s University Quetta, 87300, Pakistan
4Department of Microbiology University of Balochistan Quetta 87300 Balochistan Pakistan
چکیده
Seafoods are the main source of animal protein in our daily diet and their consumption has been increased due to its high health benefits over red meats. This study aimed to evaluate the heavy metals accumulation in a freshwater catfish muscle (Wallago attu) and the detection of heavy metal resistance genes (HMRGs) in Pseudomonas aeruginosa isolated from the fish intestine. W. attu (n = 60) was collected from four different sites (Qabula Shakh, Magsi Shakh, Umrani Shakh, and Jamali Shakh) of Pat Feeder Canal, Balochistan. The heavy metals and HMRGs were detected using atomic absorption spectrophotometer and polymerase chain reaction. The concentrations of Cd (0.27±0.001 mg/L), Fe (1.23±0.001 mg/L), and Pb (1.23±0.0005 mg/L) were found to be above the permissible limits of WHO in the samples from Jamali Shakh. Moreover, a strong Pearson’s correlation of the metal Cd was observed with Zn, Fe, Cr, Cu, Pb, and Ni. However, Zn has a strong correlation with Fe and Cr; Fe with Cu, Ni, and Cu. Cr with Pb and Cu; Cu with Ni and Pb; while Ni has a strong correlation with Pb and Mn. P. aeruginosa was also identified from 41 species out of all fish intestine specimens (68%). Similarly, different heavy metal resistance genes (MRGs) including czcA 4 (36.3%), ncc 4 (36.3%), chrR 2 (18.1%), and copA 1 (9%) were confirmed using PCR. In conclusion, Cd, Fe, and Pb concentrations were higher than the WHO permissible limits. However, other heavy metals (Ni, Zn, Cr, Cu, and Mn) were permissible limits in the fish muscle. The results of this study have shown a correlation between the buildup of heavy metals and the presence of MRGs.
کلیدواژه‌ها
Food Safety؛ Atomic Absorption Spectrometer؛ Metal Resistance؛ PCR؛ Bioaccumulation
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