News

a.z
Hemati, Z., Nabavi, A., Zamani Moghaddam, A., godarztalejerdi, A., Saei Dehkordi, S., Rahmani Dehkordi, M. (2024). Phenotypic and genotypic characterization and determination of Listeria monocytogenes antibiotic resistance profile isolated from raw milk samples, worker's hands and milking equipment in Shahrekord. , 37(1), 34-41. doi: 10.22092/vj.2023.361744.2056
Zahra Hemati; Amin Nabavi; Abdul Karim Zamani Moghaddam; Ali godarztalejerdi; Siavash Saei Dehkordi; Mehran Rahmani Dehkordi. "Phenotypic and genotypic characterization and determination of Listeria monocytogenes antibiotic resistance profile isolated from raw milk samples, worker's hands and milking equipment in Shahrekord". , 37, 1, 2024, 34-41. doi: 10.22092/vj.2023.361744.2056
Hemati, Z., Nabavi, A., Zamani Moghaddam, A., godarztalejerdi, A., Saei Dehkordi, S., Rahmani Dehkordi, M. (2024). 'Phenotypic and genotypic characterization and determination of Listeria monocytogenes antibiotic resistance profile isolated from raw milk samples, worker's hands and milking equipment in Shahrekord', , 37(1), pp. 34-41. doi: 10.22092/vj.2023.361744.2056
Hemati, Z., Nabavi, A., Zamani Moghaddam, A., godarztalejerdi, A., Saei Dehkordi, S., Rahmani Dehkordi, M. Phenotypic and genotypic characterization and determination of Listeria monocytogenes antibiotic resistance profile isolated from raw milk samples, worker's hands and milking equipment in Shahrekord. , 2024; 37(1): 34-41. doi: 10.22092/vj.2023.361744.2056

Phenotypic and genotypic characterization and determination of Listeria monocytogenes antibiotic resistance profile isolated from raw milk samples, worker's hands and milking equipment in Shahrekord

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 5, Volume 37, Issue 1, March 2024, Pages 34-41    PDF (1.15 M)
Document Type: Full Research Paper
DOI: 10.22092/vj.2023.361744.2056
Authors
Zahra Hemati* 1; Amin Nabavi2; Abdul Karim Zamani Moghaddam3; Ali godarztalejerdi4; Siavash Saei Dehkordi5; Mehran Rahmani Dehkordi2
1Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
2Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
4Department of Pathobiology, Faculty of Veterinary Medicine, Bo Ali Sina University, Hamadan, Iran
5Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Abstract
 contaminated ,milk raw as such food contaminated from humans to transmitted is ,listeriosis of cause the ,monocytogenes Listeria
 ,samples milk raw the in monocytogenes Listeria identify to conducted was research This .equipment milking contaminated or ,hands
 worker 40 and equipment milking 40 ,samples milk raw 70 ,study this In .workers dairy Shahrekord of hands and equipment milking
 Prs primers with PCR by amplified and boiling by extracted was colonies suspected the of DNA the ,confirmation biochemical ingFollow .agar Palcom on grown were they ,broth nutritional Brucella with enrichment initial an after and collected were samples hand
 the by assessed was isolates confirmed of resistance Antibiotic .)species monocytogenes Listeria (74DG69-DG and) genus Listeria(
 the on Based .assays biochemical using confirmed were) 9.33% (14 ,samples 150 Of .antibiotics five against method diffusion disc
 (0.67%) workers of sample hand one and) 0.67% (equipment milking of sample one ,)6.67% (milk raw of samples ten ,results PCR
 sample hand one and) 0.67% (equipment milking of sample one ,)5.33% (milk raw of samples eight and Listeria as identified were
 to resistance highest the exhibited isolates Confirmed .species monocytogenes Listeria for results positive gave) 0.67% (workers of
 Listeria ,Therefore .)80% (ampicillin and) 90% (amikacin to susceptibility highest the and) 60% (erythromycin and) 90% (ceftizoxime
 listeriosis of risks possible and versa vice and milk to hands' workers and equipment milking from transferred be can monocytogenes
.consumers milk raw threaten
Keywords
R; milk; monocytogenes Listeria; PCR; Listeria; agar Pal
References
  1. Abdzed, H.S., Kadhim, A.P.D.K.S. and Saleh, A.P.D.M.A., 2022. Listeria monocytogenes presence in milk and dairy products. International Journal of Scientific Trends, 1(2):15-23.
  2. Allerberger, F., Wagner, M. 2010. Listeriosis: a resurgent foodborne infection. Clinical Microbiology and Infection. 16(1):16-23.
  3. Badawy, B., Gwida, M., Sadat, A., El-Toukhy, M., Sayed-Ahmed, M., Alam, N., Ahmad, S., Ali, M.S. and Elafify, M., 2022. Prevalence and antimicrobial resistance of virulent Listeria monocytogenes and Cronobacter sakazakii in dairy cattle, the environment, and dried milk with the in vitro application of natural alternative control. Antibiotics, 11(8):1087.
  4. Borena, B.M., Dilgasa, L., Gebremedhin, E.Z., Sarba, E.J., Marami, L.M., Kelbesa, K.A. and Tadese, N.D., 2022. Listeria species occurrence and associated risk factors and antibiogram of Listeria monocytogenes in milk and milk products in Ambo, Holeta, and Bako towns, Oromia Regional State, Ethiopia. Veterinary Medicine International, (2022), 1-11.
  5. Cao, X., Wang, Y., Wang, Y. and Ye, C., 2018. Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China. Journal of Infection and Public Health, 11(1):59-63.
  6. Castro, H., Jaakkonen, A., Hakkinen, M., Korkeala, H., Lindström, M. 2018. Occurrence, persistence, and contamination routes of Listeria monocytogenes genotypes on three Finnish dairy cattle farms: a longitudinal study. Applied and environmental microbiology 84(4):e02000-17.
  7. CLSI, “Performance Standards for antimicrobial susceptibility testing; 15th informational supplement,” M100_S15, vol. 25,
  8. de Noordhout, CM., Devleesschauwer, B., Angulo, FJ., Verbeke, G., Haagsma, J., Kirk, M., et al. 2014. The global burden of listeriosis: a systematic review and meta-analysis. The Lancet Infectious Diseases. 14(11):1073-82.
  9. Drevets, DA. 1999. Dissemination of Listeria monocytogenes by infected phagocytes. Infection and immunity 67(7):3512-7.
  10. Drevets, DA., Leenen, PJ., Greenfield, RA. 2004. Invasion of the central nervous system by intracellular bacteria. Clinical microbiology reviews 17(2):323-47.
  11. Dumen, E., Baca, A.U. and Dumen, E., 2008. Comparative detection of Listeria monocytogenes in raw milk by microbiological method and PCR. Medycyna Wet, 1:59-63.
  12. Ebadifar, A.M., Jaydari, A., Shams, N. and Rahimi, H., 2022. Prevalence of Listeria monocytogenes in raw milk of the healthy cattle in Lorestan province (iran) by PCR. Journal of Advanced Biomedical Sciences. 12(2), 1-6.
  13. Farber, JM., Pagotto, F., Scherf, C. 2007. Incidence and behavior of Listeria monocytogenes in meat products. Listeria, listeriosis, and food safety 521-88.
  14. Gómez‐Laguna, J., Cardoso‐Toset, F., Meza‐Torres, J., Pizarro‐Cerdá, J., Quereda, JJ. 2020. Virulence potential of Listeria monocytogenes strains recovered from pigs in Spain. Veterinary Record 187(11):e101-e101.
  15. Grif, K., Patscheider, G., Dierich, M., Allerberger, F. 2003. Incidence of fecal carriage of Listeria monocytogenes in three healthy volunteers: a one-year prospective stool survey. European Journal of Clinical Microbiology and Infectious Diseases 22(1):16-20.
  16. Haley, BJ., Sonnier, J., Schukken, YH., Karns, JS., Van Kessel, J. 2015. Diversity of Listeria monocytogenes within a US dairy herd, 2004–2010. Foodborne Pathogens and Disease 12(10):844-50.
  17. Harshani, H.B.C., Ramesh, R., Halmillawewa, A.P. and Wijendra, W.A., 2022. Phenotypic and genotypic characterization of antibiotic resistance of Listeria monocytogenes isolated from raw milk samples collected from Polonnaruwa, Sri Lanka. Emirates Journal of Food and Agriculture. 34(1):16-25.
  18. Jamali, H., Radmehr, B. and Thong, K.L., 2013. Prevalence, characterization, and antimicrobial resistance of Listeria species and Listeria monocytogenes isolates from raw milk in farm bulk tanks. Food Control, 34(1):121-125.
  19. Jones, GS., Bussell, KM., Myers-Morales, T., Fieldhouse, AM., Bou Ghanem, , D'Orazio, SE. 2015. Intracellular Listeria monocytogenes comprises a minimal but vital fraction of the intestinal burden following foodborne infection. Infection and immunity 83(8):3146-56.
  20. Khademi, F., Sahebkar, A. 2019. A systematic review and meta-analysis on the prevalence of antibiotic-resistant Listeria species in food, animal and human specimens in Iran. Journal of food science and technology 56(12):5167-83.
  21. Kim, SW., Haendiges, J, Keller, EN., Myers, R., Kim, A., Lombard, JE., et al. 2018. Genetic diversity and virulence profiles of Listeria monocytogenes recovered from bulk tank milk, milk filters, and milking equipment from dairies in the United States (2002 to 2014). PLoS One 13(5):e0197053.
  22. Lecuit, M. 2020. Listeria monocytogenes, a model in infection biology. Cellular Microbiology. 22(4):e13186.
  23. Letchumanan, V., Wong, P.C., Goh, B.H., Ming, L.C., Pusparajah, P., Wong, S.H., Ab Mutalib, N.S. and Lee, L.H., 2018. A review on the characteristics, taxanomy and prevalence of Listeria monocytogenes. Progress In Microbes & Molecular Biology, 1(1):1-8
  24. Owusu-Kwarteng, J., Wuni, A., Akabanda, F., Jespersen, L. 2018. Prevalence and characteristics of Listeria monocytogenes isolates in raw milk, heated milk and nunu, a spontaneously fermented milk beverage, in Ghana. Beverages 4(2):40.
  25. Quereda, JJ., Morón-García, A., Palacios-Gorba, C., Dessaux, C., García-del Portillo, F., Pucciarelli, MG., et al. 2021. Pathogenicity and virulence of Listeria monocytogenes: A trip from environmental to medical microbiology. Virulence 12(1):2509-45.
  26. Rahimi, E., Ameri, M., Momtaz, H. 2010. Prevalence and antimicrobial resistance of Listeria species isolated from milk and dairy products in Iran. Food Control. 21:1448–52.
  27. Rocha, CE., Mol, JP., Garcia, LN., Costa, LF., Santos, RL., Paixão, TA. 2017. Comparative experimental infection of Listeria monocytogenes and Listeria ivanovii in bovine trophoblasts. PLoS One 12(5):e0176911.
  28. Ryser, ET. Listeria. Foodborne Infections and Intoxications: Elsevier. p. 201-20.
  29. Seyoum, ET., Woldetsadik, DA., Mekonen, TK., Gezahegn, HA., Gebreyes, WA. 2015. Prevalence of Listeria monocytogenes in raw bovine milk and milk products from central highlands of Ethiopia. The Journal of Infection in Developing Countries 9(11):1204-9.
  30. Tahoun, AB., Abou Elez, RM., Abdelfatah, EN., Elsohaby, I., El-Gedawy, AA., Elmoslemany, AM. 2017. Listeria monocytogenes in raw milk, milking equipment and dairy workers: Molecular characterization and antimicrobial resistance patterns. Journal of global antimicrobial resistance 10:264-70.
  31. Tang, J. 2013. Risk Assessment of Listeria monocytogenes in Ready-to-eat Meat from Plants to Consumption: Doctoral dissertation, Virginia Polytechnic Institute and State University.
  32. Vázquez-Boland, JA., Kuhn, M., Berche, P., Chakraborty, T., Domı́nguez-Bernal, G., Goebel, W., et al. 2001. Listeria pathogenesis and molecular virulence determinants. Clinical microbiology reviews 14(3):584-640.
  33. Wang, X., Jothikumar, N., & Griffiths, M. W. (2004). Enrichment and DNA extraction protocols for the simultaneous detection of Salmonella and Listeria monocytogenes in raw sausage meat with multiplex real-time PCR. Journal of food protection, 67(1), 189-192.
Statistics
Article View: 595
PDF Download: 504


counter
Journal management system. designed by sinaweb