| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,262 |
| تعداد مقالات | 36,057 |
| تعداد مشاهده مقاله | 55,176,198 |
| تعداد دریافت فایل اصل مقاله | 94,175,583 |
اثر پیش‌تیمار بذر بر بهبود تحمل به سرما در مرحله جوانه‌زنی برخی ژنوتیپ‌های نخود (Cicer arietinum L.) | ||
| علوم و فناوری بذر ایران | ||
| دوره 12، شماره 2 - شماره پیاپی 30، تیر 1402، صفحه 79-96 اصل مقاله (339.45 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2021.355620.1405 | ||
| نویسندگان | ||
| محمد محمدی1؛ رضا توکل افشاری* 2؛ جعفر نباتی3؛ احسان اسکوئیان4 | ||
| 1دانش آموخته کارشناسی ارشد علوم و تکنولوژی بذر، دانشکده کشاورزی، دانشگاه فردوسی مشهد. | ||
| 2استادگروه اگروتکنولوژی، دانشکده کشاورزی دانشگاه فردوسی مشهد. | ||
| 3استادیار پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد. | ||
| 4عضو هیئت علمی موسسه تحقیقات بیوتکنولوژی کشاورزی واحد مشهد (ABRII) ، سازمان تحقیقات ، آموزش و ترویج کشاورزی (AREEO) ، مشهد. | ||
| چکیده | ||
| شناسایی پیش‌تیمارهای القا کننده متابولیت‌های ثانویه به‌منظور تحمل به تنش سرما می‌تواند در استقرار و عملکرد گیاهان زراعی موثر باشد. جهت ارزیابی اثر پیش‌تیمار بذر بر تحمل سرما سه ژنوتیپ و یک رقم نخود کابلی در مرحله جوانه‌زنی پژوهشی در سال 1398 در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. تیمارها شامل دمای 5، 10، 15 و 20 درجه سلسیوس، ژنوتیپ‌ها MCC505، ILC8617، MCC495 و رقم سارال و پیش‌تیمارها شاهد (بدون پیش‌تیمار)، هیدروپرایمینگ، کلرید سدیم، اسید سالیسیلیک، نیتروپروساید سدیم، باکتری حل‌کننده فسفر و پتاسیم، طیف کامل 20 آمینواسید ضروری، نیترات پتاسیم و سولفات روی بودند. نتایج نشان داد که تیمارهای سدیم‌نیتروپروساید، هیدروپرایمینگ، اسید سالیسیلیک و سولفات روی با کاهش دما از توقف فعالیت جوانه‌زنی جلوگیری کردند و از میان آن‌ها، تیمار نیتروپروساید سدیم توانست درصد جوانه‌زنی، سرعت جوانه‌زنی، پراکسید هیدروژن و کاتالاز با کاهش دما به 5 درجه سلسیوس نسبت به شاهد به ترتیب 7/5، 19، 4 و 15 درصد بهبود دهد. علاوه بر آن، تیمار مذکور در شرایط دمای 20 درجه سلسیوس نیز در صفات آلفاآمیلاز و پراکسید هیدروژن به ترتیب منجر به بهبود 2 و 7/4 درصدی نسبت به شاهد شد. درمجموع، اثر پیش‌تیمارها بر تحمل به سرما در مرحله جوانه‌زنی، مشخص کرد که تنش سرما باعث کاهش جوانه‌زنی بذر و سرعت جوانه‌زنی می‌شود و اثر منفی بر شاخص‌های جوانه‌زنی، فعالیت آنزیمی و بیوشیمیایی دارد. اما کاربرد پیش‌تیمارها به‌ویژه نیتروپروساید سدیم اثر تنش سرمایی را تعدیل کرد و باعث بهبود ویژگی‌های بذر تحت تنش سرما شد. | ||
| کلیدواژه‌ها | ||
| پراکسید هیدروژن؛ اسید سالیسیلیک؛ نیتروپروساید سدیم؛ هیدروپرایمینگ | ||
| عنوان مقاله [English] | ||
| The effects of seed priming on improvement of cold tolerance in germination stage of some in pea genotypes. | ||
| نویسندگان [English] | ||
| mohammad mohammadi1؛ Reza Tavakkol Afshari2؛ Jafar Nabati3؛ Ehsan Oskoueian4 | ||
| 1MS. Student of Seed Tec,hnology, Faculty of Agriculture, Ferdowsi University of Mashhad. | ||
| 2Member of staff, Faculty of Agriculture, Ferdowsi University of Mashhad. | ||
| 3Member of staff, Ferdowsi University of Mashhad, Research Center for Plant Sciences. | ||
| 4Member of staff, Mashhad Branch Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Mashhad. | ||
| چکیده [English] | ||
| Identification of primings that induce secondary metabolites in order to withstand cold stress can be effective in the establishment and yield of crops. This experiment was conducted in the laboratory conditions as a completely randomized design with three replications to evaluate the effect of seed priming on cold tolerace of three genotypes and one cultivar of chickpea during germination stage in 2020. Treatments include temperatures of 5, 10, 15 and 20 ° C, genotypes of MCC505, ILC8617, MCC495 and Saral cultivar and primings included control (non-primed), hydropriming, sodium chloride, acid salicylic, sodium nitroprusside, phosphorus and potassium soluble bacteria, Full spectrum of 20 essential amino acids, potassium nitrate, zinc sulfate. Results indicated that the treatments of sodium nitroprusside, hydropriming, acid salicylic and zinc sulfate prevented the germination activity from stopping due to the decrease in temperature and among them, sodium nitroprusside treatment was able to improve germination percentage, germination rate, hydrogen peroxide and catalase under 5 ° C compared to the control by 5.7, 19, 4 and 15%, respectively. In addition, the above treatmens under 20 ° C resulted in 2 and 4.7% improvement in alpha-amylase and hydrogen peroxide, respectively. Generally, the effect of priming on cold tolerance at the germination stage showed that cold stress reduces seed germination and germination rate and has a negative effect on germination indices, enzymatic and biochemical activity. However, the use of primings, especially sodium nitroprusside, moderated the effect of cold stress and improved seed characteristics under cold stress. | ||
| کلیدواژه‌ها [English] | ||
| Hydrogen peroxide, Acid salicylic, Sodium nitroprusside, Hydropriming | ||
| مراجع | ||
Achard, P., F. Gong, S. Cheminant, M. Alioua, P. Hedden, and P. Genschik. 2008. The cold-inducible CBF1 factor–dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism. Plant Cell. 20(8):2117-2129.Agrawal, R. L. 2003. Seed technology. Publication. Co. PVT. LTD. New Delhi India. 64:229-236. Amooaghaie, R., and K. Nikzad. 2013. The role of nitric oxide in priming-induced low-temperature tolerance in two genotypes of tomato. Seed Science Res. 23(2):123-131. Arc, E., J. Sechet, F. Corbineau, L. Rajjou, and A. Marion-Poll. 2013. ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Front. Plant Sci. 4(63): 1-19. Arif, M., M. Waqas, K. Nawab, and M. Shahid. 2007. Effect of seed priming in Zn solutions on chickpea and wheat. Afr Crop Sci Conf Proc. 8:237-240. Cheng, X.Y., Y. Wu, J.P. Guo, B. Du, R.Z. Chen, L.L. Zhu, and G.C. He. 2013. A rice lectin receptor-like kinase that is involved in innate immune responses also contributes to seed germination. Plant J. 76:687-698. Bahmani, M., R. Maali-Amiri, M. Javan-Nikkhah, O. Atghia, and A.J.R. Rasolnia. 2020. Enhanced Tolerance to Ascochyta Blight in Chickpea Plants via Low Temperature Acclimation. Rus J. Plant Physiol. 67(4):758-766. Basra, S. M.A., M. Farooq, R. Tabassam, and N. Ahmad. 2005. Physiological and biochemical aspects of pre-sowing seed treatments in fine rice (Oryza sativa L.). Seed Sci. Technol. 33(3):623-628. Beihaghi, M., A. Bagheri, A.R. Bahrami, F. Shahriari, and A. Nezami. 2010. The possible role of Phosphoenolpyrovate Carboxykinase (PEPCK) in protein content of chickpea seeds (Cicer arietinum L.). Iranian J. Pulses Res. 1(1):57-64. Bialecka, B., and J. Kepczynski. 2010. Germination, alpha-, beta-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress in the presence of ethephon or gibberellin A3. Acta Biol Cracoviensia S. Botanica. 52(1):7-12. Čanak, P., M. Mirosavljević, M. Ćirić, B. Vujošević, J. Kešelj, D. Stanisavljević, and B. Mitrović. 2016. Seed priming as a method for improving maize seed germination parameters at low temperatures. Ratarstvo i povrtarstvo. 53(3):106-110. Copeland, L., and M. McDonald. 1995. Principals of seed science and technology. Chapman and Hall. Delledonne, M. 2005. NO news is good news for plants. Curr. Opin. plant Biol. 8(4):390-396. Dhindsa, R. S., P. Plumb-Dhindsa, and T.A. Thorpe. 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot. 32(1):93-101. Ebadi, A., and S.K. Gollojeh. 2009. Effects of seed priming on growth and yield of chickpea under saline soil. Rec. Res. Sci. Technol. 1(6): 282-286. Ellis, R., and E. Roberts. 1980. Improved equations for the prediction of seed longevity. Ann. Bot. 45(1):13-30. FAO. 2019. FAO Year Book [Online]. Available at http:/faostat.fao.org/site/. Farahbakhsh, H. 2012. Germination and seedling growth in unprimed and primed seeds of fennel as affected by reduced water potential induced by NaCl. Int. Res J. Appl. Basic Sci. 3(4):737-744. Finch-Savage, W., K. Dent, and L. Clark. 2004. Soak conditions and temperature following sowing influence the response of maize (Zea mays L.) seeds to on-farm priming (pre-sowing seed soak). Field Crops Res. 90(2-3):361-374. Galandari, S. 2015. seed priming. Int. conf. Res. Sci. Technol Kualalumpur -malaysia. 14th December 2015. Ghaderi far, A., E. Soltani, A. Soltani, and A.A. MIRI. 2008. Effect of priming on response of germination to temperature in cotton. J. Agric Sci. Nat. Res. 15(3):44-51. Ghasemi , R., A. Bagheri, N. Moshtaghi, and F. Shokouhi. 2017. The pattern of CBF and P5CS genes expression under freezing stress and effects of cold acclimation on free proline changes in chickpea (Cicer arietinum). Iranian J. Pulses Res. 8(2):31-43. Ghassemi-Golezani, K., A.A. Aliloo, M. Valizadeh, and M. Moghaddam. 2008. Effects of different priming techniques on seed invigoration and seedling establishment of lentil (Lens culinaris Medik). J. Food Agric. 6(2):222. Halmer, P. 2004. Methods to improve seed performance in the field. Pp 125-166. In R.L. Benech-Arnold and R.A. Sanchez(eds.)Handbook of seed physiology: applications to agricultures. Food Product Press, New York. Hasegawa, P. M., R.A. Bressan, J.K. Zhu, and H.J. Bohnert. 2000. Plant cellular and molecular responses to high salinity. Ann. Rev. Plant Biol. 51(1):463-499. Hayat, S., S. Yadav, M. Alyemeni, and A. Ahmad. 2014. Effect of sodium nitroprusside on the germination and antioxidant activities of tomato (Lycopersicon esculentum Mill). Bulg J. Agric Sci. 20(1):140-144. Hiremath, U., B. Gowda, G. Lokesh, and B. Ganiger. 2021. Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.). J. Appl Nat Sci. 13(2):735-743. Hung, S.H., Y. Chih-Wen, and C.H. Lin. 2005. Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sinica. 46. Khodabakhsh, F., R. Amooaghaie, A. Mostajeran, and G. Emtiazi. 2011. Effect of hydro and osmopriming in Two commercial chickpea (Cicer arietinum L.) cultivars on germination, growth parameters and nodules number in salt stress condition. Iranian J. Plant Biol. 2(4):71-86.(In Persian) Khorshidi, M., and A. Nojavan. 2006. The effects of abscisic acid and CaCl2 on the activities of antioxidant enzymes under cold stress in maize seedlings in the dark. Pakistan J. Biol Sci. 9:54-59. Li, H., H.B. Niu, J. Yin, M.B. Wang, H.B. Shao, D.Z. Deng, X. Chen, J.P. Ren, and Y.C. Li. 2008. Protective role of exogenous nitric oxide against oxidative-stress induced by salt stress in barley (Hordeum vulgare). Colloids Surfaces B: Biointerfaces. 65(2):220-225. Liu, H.Y., X. Yu, D.Y. Cui, M.H. Sun, W.N. Sun, Z.C. Tang, S. Kwak, and W.A. Su. 2007. The role of water channel proteins and nitric oxide signaling in rice seed germination. Cell Res. 17(7):638-649. Mahakham, W., A.K. Sarmah, S. Maensiri, and P. Theerakulpisut. 2017. Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles. Sci Rep. 7:8263-8272. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trend. Plant Sci. 7(9):405-410. Moynihan, M.R., A. Ordentlich, and I. Raskin. 1995. Chilling-induced heat evolution in plants. Plant Physiol. 108(3):995-999. Neill, S.J., R. Desikan, A. Clarke, and J.T. Hancock. 2002. Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells. Plant Physiol. 128(1):13-16. Qiao, W., C. Li, and L.M. Fan. 2014. Cross-talk between nitric oxide and hydrogen peroxide in plant responses to abiotic stresses. Environ. Exp. Bot. 100:84-93. Randhir, R., and K. Shetty. 2005. Developmental stimulation of total phenolics and related antioxidant activity in light- and dark-germinated corn by natural elicitors. Process Biochem. 40:1721–1732. Saeed, A., R. Darvishzadeh, H. Hovsepyan, and A.J. Asatryan. 2010. Tolerance to freezing stress in Cicer accessions under controlled and field conditions. Afr. J. Biotechnol. 9(18):2618-2626. Scheel, D. 1998. Resistance response physiology and signal transduction. Curr. Opin Plant Biol. 1(4):305-310. Sergiev, I., V. Alexieva, and E. Karanov. 1997. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Compt. Rend. Acad. Bulg. Sci. 51(3):121-124. Singh, A., R. Gupta, and R. Pandey. 2016. Rice seed priming with picomolarrutin enhances rhiospheric Bacillus subtilis CIM colonization and plant growth. PLoS One. 11(1):e0146013. Singh, K., R. Malhotra, and M.J. Saxena. 1995. Additional sources of tolerance to cold in cultivated and wild Cicer species. Crop Sci. 35(5):1491-1497. Šírová, J., M. Sedlářová, J. Piterková, L. Luhová, and M. Petřivalský. 2011. The role of nitric oxide in the germination of plant seeds and pollen. Plant Sci. 181(5):560-572. Tabatabaei, S.A., and O. Ansari. 2018. Quantification of safflower (Carthamus tinctorius) seed germination response to water potential and priming: hydro time models on the basis of normal, weibull and gumbel distributions. Environ. Stresses Crop Sci. 11(2):327-340. Taylor, A., and G. Harman. 1990. Concepts and technologies of selected seed treatments. Ann. Rev. Phytopathol. 28(1):321-339. Xiao, Z., R. Storms, and A. Tsang. 2006. A quantitative starch? Iodine method for measuring alpha-amylase and glucoamylase activities. Analytical Biochem. 351(1):146-148. | ||
|
آمار تعداد مشاهده مقاله: 765 تعداد دریافت فایل اصل مقاله: 506 |
||