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Investigation of the effect of CMC and alcohol and temperature on thermolysin enzyme stability | ||
| تحقیقات مهندسی صنایع غذایی | ||
| Article 8, Volume 21, Issue 2 - Serial Number 73, March 2023, Pages 91-102 PDF (521.79 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/fooder.2023.354142.1306 | ||
| Authors | ||
| Amir Agheli kohne shahri1; Yaghub Pazhang2; fatemeh ghamari* 3 | ||
| 1Department of Biochemistry, Biotechnology Research Institute, Urmia University.Urmia.Iran. | ||
| 2Department of Biology, Faculty of Science, urmia University,Urmia,Iran. | ||
| 3Assistant professor Department of science payame noor university ,P.O.Box 19395-4697,Tehran,Iran | ||
| Abstract | ||
| Thermolysin enzyme is a heat-resistant metalloproteinase enzyme that binds (EC 3.4.24.4) a peptide containing hydrophobic amino acid leucine and phenylalanine. This enzyme`s immobilization is very important. Enzyme immobilization enhances the stability and reuse of the enzyme and improves the properties of the enzyme and facilitates the enzyme readiness of the product. In order to study the adsorption in the presence of ethanol, different concentrations of ethanol were investigated with enzyme. To investigate the presence of CMC, the enzyme was mixed with different percentages of CMC solution and the casein (substrate) was mixed with different amounts. The effect of CMC and alcohol on the enzymatic solution of CMC was obtained. The effect of CMC and alcohol was investigated. The effect of CMC 2%, 4%, 6%, 8% and 10% with 1% casein on the enzyme was investigated. In this study, it was shown that thermolysin increases activity and stability against temperature rise and then decreases at very high temperatures, and alcohol causes enzyme instability. CMC increases the activity and stability of the thermolysin enzyme, but the presence of CMC can increase the stability and resistance of the thermolysin enzyme to alcohol. CMC has been shown to stabilize thermolysin. | ||
| Keywords | ||
| Enzyme Stability"; Thermolysin"; Carboxymethyl Cellulose"; "; Alcohol | ||
| References | ||
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