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Karjo, B., Fattahi, M. (2023). Effect of salicylic acid stimulant on the biomass content and some biochemical characteristics of deadly nightshade (Atropa belladonna L.) hairy roots. , 30(2), 263-277. doi: 10.22092/ijrfpbgr.2023.360647.1427
B. Karjo; M. Fattahi. "Effect of salicylic acid stimulant on the biomass content and some biochemical characteristics of deadly nightshade (Atropa belladonna L.) hairy roots". , 30, 2, 2023, 263-277. doi: 10.22092/ijrfpbgr.2023.360647.1427
Karjo, B., Fattahi, M. (2023). 'Effect of salicylic acid stimulant on the biomass content and some biochemical characteristics of deadly nightshade (Atropa belladonna L.) hairy roots', , 30(2), pp. 263-277. doi: 10.22092/ijrfpbgr.2023.360647.1427
Karjo, B., Fattahi, M. Effect of salicylic acid stimulant on the biomass content and some biochemical characteristics of deadly nightshade (Atropa belladonna L.) hairy roots. , 2023; 30(2): 263-277. doi: 10.22092/ijrfpbgr.2023.360647.1427

Effect of salicylic acid stimulant on the biomass content and some biochemical characteristics of deadly nightshade (Atropa belladonna L.) hairy roots

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 8, Volume 30, Issue 2 - Serial Number 60, March 2023, Pages 263-277    PDF (992.8 K)
Document Type: Research Paper
DOI: 10.22092/ijrfpbgr.2023.360647.1427
Authors
B. Karjo1; M. Fattahi* 2
1M.Sc. Graduated. Dept. Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, I.R. Iran.
2Corresponding author, Assoc. Prof., Dept. Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, I.R. Iran
Abstract
The rapid and mass production of secondary metabolites through chemical methods is mostly expensive, difficult, or impossible. Considering the economic importance of these metabolites and their small production in medicinal plants, some methods such as the cultivation of hairy roots and the use of biotic and abiotic stimuli have been suggested to increase these metabolites. The accumulation of the tropane alkaloid atropine in Atropa belladonna L. is of great importance in the pharmaceutical industry. In this research, transgenic hairy roots of A. belladonna was produced using an A7 strain of Agrobacterium rhizogenes. The transformation of hairy roots was confirmed by PCR test. The results of the PCR test showed the correctness of the amplification of the bp780 fragment for the rolB gene in hairy roots. In addition, the effect of different concentrations (0, 125, 250, and 500 μM) of salicylic acid in two times of 24 and 48 hours on the amount of atropine content, the plant growth rate and the activity of antioxidant enzymes of A. belladonna hairy roots was investigated. The data statistically analyzed using factorial experiment based on a completely random design with three replications. The results of the analysis of variance showed significant effect of salicylic acid on all the traits (P<0.01). The effect of time was significant for the amount of atropine and ascorbate peroxidase (APX). The salicylic acid by time interaction effects were significant for the amount of atropine, catalase (CAT), and guaiacol peroxidase (GPX) (P<0.01, 0.05). The results showed that by increasing salicylic acid concentrations the growth of hairy roots significantly decrease compared to the control and the lowest values of fresh and dry weight of the hairy roots (2.79 and 0.148 g) were obtained in 500 μM of salicylic acid compared to the control (6.76 and 0.273 g). However, based on GC/MS analysis, the highest amount of atropine (11 times more than the control) was obtained in the 250 μM salicylic acid in a period of 24 hours. The highest catalase and guaiacol peroxidase activities were obtained at concentrations of 250 and 125µM salicylic acid for 24 hours, respectively. The highest level of ascorbate peroxidase enzyme activity was obtained at the concentration of 250 µM salicylic acid compared to the control. It was concluded that the salicylic acid stimulant at low concentrations is useful for cell signaling and stimulating the production of secondary metabolites, but at high concentrations, it disrupts the growth of hairy roots.
 
Keywords
Agrobacterium rhizogenes; alkaloid; elicitor; antioxidant enzymes; hairy root
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