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شناسایی کنههای ناقل Cercopithifilaria و Hepatozoon در سگهای مناطق مختلف ایران | ||
| نامه انجمن حشره شناسی ایران | ||
| دوره 43، شماره 1، اردیبهشت 1402، صفحه 53-61 اصل مقاله (1011.12 K) | ||
| نوع مقاله: مقاله کامل، فارسی | ||
| شناسه دیجیتال (DOI): 10.52547/JESI.43.1.6 | ||
| نویسندگان | ||
| زهرا بحیرایی1؛ علیرضا سازمند* 1؛ علیرضا نوریان1؛ دومنیکو اوترانتو2 | ||
| 1گروه آموزشی پاتوبیولوژی، دانشکده پیرادامپزشکی، دانشگاه بوعلی سینا، همدان، ایران | ||
| 2انستیتو انگل شناسی، دپارتمان دامپزشکی، دانشگاه باری آلدو مورو، ایتالیا | ||
| چکیده | ||
| پیرو یافتن تک‌یاختهی Hepatozoon canis و نماتود Cercopithifilaria bainae در سگهای ایران، این مطالعه با هدف تشخیص ناقلین بالقوهی این دو انگل در میان کنههای جمعآوری شده از سگهای ۵ استان ایران انجام شد. از مهر ماه 1397 تا خرداد ماه 1401 تعداد 222 قلاده سگ صاحبدار و 541 قلاده سگ ولگرد از استانهای همدان (305 قلاده)، کرمانشاه (227 قلاده)، یزد (80 قلاده)، خوزستان (71 قلاده) و مازندران (80 قلاده) جهت آلودگی به کنه مورد بررسی قرار گرفتند. کنههای جمع آوری شده (3339 عدد) از نظر ریختشناسی شناسایی شده و کنههای بالغ با استفاده از روشهای PCR معمولی (119 کنه)، کالبدگشایی (106 کنه) و بررسی میکروسکوپی اسلاید تهیه شده از مقاطع بافتی فرمالینه (102 کنه) آزمایش شدند. در مجموع 169 قلاده سگ (1/22%) به کنههای سخت آلودگی داشتند. فراوانی آلودگی در همدان 4/32%، کرمانشاه 3/27% و یزد 10% بود درحالیکه آلودگی در سگهای مورد بررسی در مازندران و خوزستان مشاهده نشد. اغلب کنههای نر جمعآوری شده متعلق به گونهRhipicephalus sanguineus (3/89%) و Rhipicephalus turanicus بودند اما یک قلاده سگ از همدان به ۲ کنهی Hyalomma asiaticum (۱ نر و ۱ ماده) و ۱ کنهی نر Hyalomma excavatumآلوده بود. طبق بررسی مولکولی فراوانی Cercopithifilaria،Hepatozoon و آلودگی همزمان به هر دو انگل در کنههای Rhipicephalusبه ترتیب 28/14%، 7/6% و 68/1% بود. انگلهای مورد مطالعه در کالبدگشایی و بررسی هیستولوژیک کنهها مشاهده نشدند. نتایج توالییابی و آنالیز فیلوژنتیکی مؤید حضور C. bainae و H. canis در کنههای گونهRh. sanguineus و Rh. turanicus بود. نتایج این مطالعه مؤید آن است که هر دو گونهی کنه در اپیدمیولوژی Cercopithifilaria و Hepatozoon در ایران نقش دارند. | ||
| کلیدواژه‌ها | ||
| پاتوژنهای منتقله از ناقل؛ ریپی سفالوس تورانیکوس؛ ریپی سفالوس سنگویینئوس؛ سرکوپیتی فیلاریا؛ هپاتوزوئون | ||
| عنوان مقاله [English] | ||
| Identification of Hepatozoon and Cercopithifilaria in ticks infesting dogs of different regions of Iran | ||
| نویسندگان [English] | ||
| Zahra Bahiraei1؛ Alireza Sazmand1؛ Alireza Nourian1؛ Domenico Otranto2 | ||
| 1Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran | ||
| 2Department of Veterinary Medicine, University of Bari, Bari, Italy | ||
| چکیده [English] | ||
| Following previous detection of Hepatozoon canis and Cercopithifilaria bainae in dogs from Iran, this study aimed to identify potential tick vectors collected from dogs from five provinces of Iran. From October 2018 to September 2021, a total of 222 owned and 541 sheltered dogs from Hamedan (n=305), Kermanshah (n = 227), Yazd (n =80), Khouzestan (n =71), and Mazandaran (n =80) were examined for ticks. Collected ticks (n =3339) were identified morphologically, and adults were screened for the detection of Hepatozoon and Cercopithifilaria either with conventional PCR (n=119 ticks), tick dissection (n=106), or microscopical examination after fixation in formalin (n =102). A total of 169 dogs (22.1%) were infested with ticks. Tick infestation rate varied in different provinces (i.e., 32.4% in Hamedan, 27.3% in Kermanshah, 10.0% in Yazd), but no ticks were collected in dogs from Mazandaran and Khouzestan. The majority of male ticks were identified as Rhipicephalus sanguineus sensu lato (89.3%) and Rhipicephalus turanicus, but one dog from Kermanshah was infested with Hyalomma asiaticum (1 male and 1 female) and Hyalomma excavatum (1 male). At the molecular examination, Cercopithifilaria was detected in 14.28%, Hepatozoon in 6.7% ticks, and both parasites in 1.68% of Rhipicephalus ticks. Parasites were not observed at tick dissection and histological examinations. Nucleotide sequencing and phylogenetic analyses revealed C. bainae and H. canis in R. sanguineus sensu lato and R. turanicus ticks. Data suggests that both tick species above may play a role in the epidemiology of Cercopithifilaria and Hepatozoon in Iran. | ||
| کلیدواژه‌ها [English] | ||
| Tick, Filarial nematode, Protozal parasite, Iran, Rhipicephalus, vector-borne pathogens | ||
| مراجع | ||
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