اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,074
تعداد مقالات34,305
تعداد مشاهده مقاله47,212,602
تعداد دریافت فایل اصل مقاله78,130,996
Shahveh, M, Tajbakhsh, E, Momtaz, H, Ranjbar, R. (1402). Molecular Characterization of Enterococcus faecalis and Enterococcus faecium Isolated from a Meat Source in Shahrekord Local Markets, Iran. سامانه مدیریت نشریات علمی, 78(4), 1387-1396. doi: 10.32592/ARI.2023.78.4.1387
M Shahveh; E Tajbakhsh; H Momtaz; R Ranjbar. "Molecular Characterization of Enterococcus faecalis and Enterococcus faecium Isolated from a Meat Source in Shahrekord Local Markets, Iran". سامانه مدیریت نشریات علمی, 78, 4, 1402, 1387-1396. doi: 10.32592/ARI.2023.78.4.1387
Shahveh, M, Tajbakhsh, E, Momtaz, H, Ranjbar, R. (1402). 'Molecular Characterization of Enterococcus faecalis and Enterococcus faecium Isolated from a Meat Source in Shahrekord Local Markets, Iran', سامانه مدیریت نشریات علمی, 78(4), pp. 1387-1396. doi: 10.32592/ARI.2023.78.4.1387
Shahveh, M, Tajbakhsh, E, Momtaz, H, Ranjbar, R. Molecular Characterization of Enterococcus faecalis and Enterococcus faecium Isolated from a Meat Source in Shahrekord Local Markets, Iran. سامانه مدیریت نشریات علمی, 1402; 78(4): 1387-1396. doi: 10.32592/ARI.2023.78.4.1387

Molecular Characterization of Enterococcus faecalis and Enterococcus faecium Isolated from a Meat Source in Shahrekord Local Markets, Iran

Archives of Razi Institute
مقاله 26، دوره 78، شماره 4، مهر و آبان 2023، صفحه 1387-1396    اصل مقاله (531.81 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2023.78.4.1387
نویسندگان
M Shahveh1؛ E Tajbakhsh* 1؛ H Momtaz1؛ R Ranjbar2
1Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Enterococcus faecalis (E. faecalis) and E. faecium (E. faecium) are commensals of the gastrointestinal biota of humans and animals and are considered opportunistic pathogens. This study aimed to improve the knowledge about E. faecalis and E. faecium isolated from meat. To this end, 104 meat samples were collected from sheep, goats, cattle, and calves from Shahrekord local markets in Iran. Presumptive colonies of E. faecalis and E. faecium were isolated from the samples and subjected to identification tests. Antimicrobial susceptibility was determined using the Kirby-Bauer disc diffusion method, and polymerase chain reaction (PCR) was performed to detect some virulence genes. Furthermore, randomly amplified polymorphic DNA typing and repetitive element sequence-based PCR fingerprinting were used to show the clonal relatedness of the isolates. The results revealed that enterococci were present in 90 out of 104 samples. Specifically, E. faecalis and E. faecium were the commonly isolated species, with the predominance of E. faecalis, which exhibited high resistance to streptomycin (95%) but was susceptible to vancomycin (85.6%). Virulence genes detection showed that ccf and cpd genes were the most prevalent genes in both species. In addition, the molecular typing method indicated that the isolates belonged to separate subgroups. This study shows the contamination of meat products by potential pathogens and resistant enterococci. There is a need to implement regular surveillance to monitor the emergence of antimicrobial-resistant E. faecalis and E. faecium in food, particularly in meat production.
کلیدواژه‌ها
Antimicrobial susceptibility؛ Gastrointestinal biota؛ Meat؛ Pathogens؛ Polymerase chain reaction
عنوان مقاله [English]
خصوصیات مولکولی انتروکوکوس فکالیس و انتروکوکوس فاسیوم جدا شده از منابع گوشتی فروشگاه های شهرکرد
سایر فایل های مرتبط با مقاله
مراجع
  1. Ramsey M, Hartke A, Huycke M. The physiology and metabolism of enterococci. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection. 2014.
  2. Kim YB, Seo KW, Shim JB, Son SH, Noh EB, Lee YJ. Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock. Poult Sci. 2019;98(11):5892-9.
  3. Nowakiewicz A, Ziolkowska G, Troscianczyk A, Zieba P, Gnat S. Determination of resistance and virulence genes in Enterococcus faecalis and E. faecium strains isolated from poultry and their genotypic characterization by ADSRRS-fingerprinting. Poult Sci. 2017;96(4):986-96.
  4. Hasanpour F, Neyestani Z, Arzanlou M, Moradi-Asl E, Sahebkar A, Khademi F. Vancomycin-resistant enterococci in Iran: A systematic review and meta-analysis of non-clinical studies. Gene Rep. 2021;24:101265.
  5. Bednorz C, Guenther S, Oelgeschlager K, Kinnemann B, Pieper R, Hartmann S, et al. Feeding the probiotic Enterococcus faecium strain NCIMB 10415 to piglets specifically reduces the number of Escherichia coli pathotypes that adhere to the gut mucosa. Appl Environ Microbiol. 2013;79(24):7896-904.
  6. Golob M, Pate M, Kusar D, Dermota U, Avbersek J, Papic B, et al. Antimicrobial Resistance and Virulence Genes in Enterococcus faecium and Enterococcus faecalis from Humans and Retail Red Meat. Biomed Res Int. 2019;2019:2815279.
  7. Chajęcka-Wierzchowska W, Zadernowska A, Łaniewska-Trokenheim Ł. Virulence factors of Enterococcus spp. presented in food. LWT. 2017;75:670-6.
  8. Ogier JC, Serror P. Safety assessment of dairy microorganisms: the Enterococcus genus. Int J Food Microbiol. 2008;126(3):291-301.
  9. Tyson GH, Nyirabahizi E, Crarey E, Kabera C, Lam C, Rice-Trujillo C, et al. Prevalence and Antimicrobial Resistance of Enterococci Isolated from Retail Meats in the United States, 2002 to 2014. Appl Environ Microbiol. 2018;84(1).
  10. Fisher K, Phillips C. The ecology, epidemiology and virulence of Enterococcus. Microbiology (Reading). 2009;155(Pt 6):1749-57.
  11. Franz CM, Stiles ME, Schleifer KH, Holzapfel WH. Enterococci in foods--a conundrum for food safety. Int J Food Microbiol. 2003;88(2-3):105-22.
  12. Maleki P, Tajbakhsh E, Rahimi E. Prevalence of ebp A, ebp B and ebp C genes in Enterococcus faecalis producing biofilm isolated from meat in Shahrekord. Navid No. 2018;21(66):41-51.
  13. Iweriebor BC, Obi LC, Okoh AI. Virulence and antimicrobial resistance factors of Enterococcusspp. isolated from fecal samples from piggery farms in Eastern Cape, South Africa. BMC Microbiol. 2015;15:136.
  14. Green MR, Sambrook J. Molecular cloning. A Laboratory Manual 4th. 2012.
  15. Eaton TJ, Gasson MJ. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol. 2001;67(4):1628-35.
  16. Tatsing Foka FE, Ateba CN. Detection of Virulence Genes in Multidrug Resistant Enterococci Isolated from Feedlots Dairy and Beef Cattle: Implications for Human Health and Food Safety. Biomed Res Int. 2019;2019:5921840.
  17. Togay SO, Keskin AC, Acik L, Temiz A. Virulence genes, antibiotic resistance and plasmid profiles of Enterococcus faecalis and Enterococcus faecium from naturally fermented Turkish foods. J Appl Microbiol. 2010;109(3):1084-92.
  18. Banerjee T. Random Amplified Polymorphic DNA (RAPD) Typing of Multidrug Resistant Enterococcus faecium Urinary Isolates from a Tertiary Care Centre, Northern India. J Clin Diagn Res. 2013;7(12):2721-3.
  19. Soodmand J, Zeinali T, Kalidari G, Hashemitabar G, Razmyar J. Antimicrobial susceptibility profile of Enterococcus species isolated from companion birds and poultry in the Northeast of Iran. Arch Razi Inst. 2018;73(3):207-13.
  20. Kim MH, Moon DC, Kim SJ, Mechesso AF, Song HJ, Kang HY, et al. Nationwide Surveillance on Antimicrobial Resistance Profiles of Enterococcus faecium and Enterococcus faecalis Isolated from Healthy Food Animals in South Korea, 2010 to 2019. Microorganisms. 2021;9(5).
  21. Kwon KH, Hwang SY, Moon BY, Park YK, Shin S, Hwang CY, et al. Occurrence of antimicrobial resistance and virulence genes, and distribution of enterococcal clonal complex 17 from animals and human beings in Korea. J Vet Diagn Invest. 2012;24(5):924-31.
  22. Seputiene V, Bogdaite A, Ruzauskas M, Suziedeliene E. Antibiotic resistance genes and virulence factors in Enterococcus faecium and Enterococcus faecalis from diseased farm animals: pigs, cattle and poultry. Pol J Vet Sci. 2012;15(3):431-8.
  23. Abouelnaga M, Lamas A, Quintela-Baluja M, Osman M, Miranda JM, Cepeda A, et al. Evaluation of the extent of spreading of virulence factors and antibiotic resistance in Enterococci isolated from fermented and unfermented foods. Ann Microbiol. 2016;66(2):577-85.
  24. Heidari H, Emaneini M, Dabiri H, Jabalameli F. Virulence factors, antimicrobial resistance pattern and molecular analysis of Enterococcal strains isolated from burn patients. Microb Pathog. 2016;90:93-7.
  25. Sharifi Y, Hasani A, Ghotaslou R, Varshochi M, Hasani A, Aghazadeh M, et al. Survey of Virulence Determinants among Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolated from Clinical Specimens of Hospitalized Patients of North west of Iran. Open Microbiol J. 2012;6:34-9.
  1. Cobo Molinos A, Abriouel H, Omar NB, Lopez RL, Galvez A. Detection of ebp (endocarditis- and biofilm-associated pilus) genes in enterococcal isolates from clinical and non-clinical origin. Int J Food Microbiol. 2008;126(1-2):123-6.
  2. Tendolkar PM, Baghdayan AS, Gilmore MS, Shankar N. Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. Infect Immun. 2004;72(10):6032-9.
  3. Shokoohizadeh L, Ekrami A, Labibzadeh M, Ali L, Alavi SM. Antimicrobial resistance patterns and virulence factors of enterococci isolates in hospitalized burn patients. BMC Res Notes. 2018;11(1):1.
  4. Werner G, Willems RJ, Hildebrandt B, Klare I, Witte W. Influence of transferable genetic determinants on the outcome of typing methods commonly used for Enterococcus faecium. J Clin Microbiol. 2003;41(4):1499-506.
آمار
تعداد مشاهده مقاله: 2,741
تعداد دریافت فایل اصل مقاله: 8,033


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب