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تاثیر زوال بذر بر صفات فیزیولوژیک و آنزیم‌های آنتی‌اکسیدانی و اس-نیتروزیلاسیون پروتئین‌ها در دورقم کلزا (Brassica napus L.) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 2، دوره 12، شماره 1 - شماره پیاپی 29، اردیبهشت 1402، صفحه 15-28 اصل مقاله (1.97 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2020.342885.1343 | ||
| نویسندگان | ||
| مریم زمانیان1؛ رضا توکل افشاری* 2؛ جعفر نباتی3؛ علیرضا سیفی4 | ||
| 1گروه اگروتکنولوژی/ دانشکده کشاورزی / دانشگاه فردوسی مشهد | ||
| 2استاد | ||
| 3استادیار پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد | ||
| 4استادیار گروه بیوتکنولوژی وبه نژادی گیاهی، دانشگاه فردوسی مشهد | ||
| چکیده | ||
| این مطالعه به صورت آزمایش فاکتوریل بر پایه طرح کاملاً تصادفی با دو عامل رقم (مودنا و اوکاپی) ، سطح پیری (صفر و 24 و 72 ساعت در دمای 40 درجه سانتی‌گراد و رطوبت اشباع) و چهار تکرار برای ارزیابی سرعت و درصد جوانه‌زنی (GP)، شاخص بنیه (SV)، هدایت‌الکتریکی (EC)، فعالیت کاتالاز(CAT)، آسکوربات‌پراکسیداز (APX)، گلوتاتیون‌ردوکتاز (GR) و اس-نیتروزوتیول (SNO) در سال 1399-1398 در دانشگاه فردوسی مشهد انجام شد. تیمارهای پیری تسریع شده (AA) باعث کاهش معنی‌دار کلیه صفات (به جز EC) در بذرهای کلزا شدند. رقم مودنا در صفات جوانه‌زنی قوی‌تر و رقم اوکاپی در فعالیت‌های بیوشیمیایی برتر بود که این را می‌توان با تفاوت ژنتیکی ارقام توجیه کرد. بر اساس نتایج، در تیمارهای 72 ساعت اکاپی نسبت به شاهد، 86% GP، 88% CAT، %89 APX و 36% SNOها کاهش یافته است. هم‌چنین، همبستگی مثبتی بین SNOها و آنزیم‌های آنتی‌اکسیدانی وجود داشت؛ به عنوان نتیجه‌گیری، نیتریک اکسید (NO)به طور غیرمستقیم و به وسیله SNOها بر آنزیم‌های آنتی-اکسیدانی در بذرهای پیرشده کلزا تاثیرگذار است. پیری بذر با کاهش فعالیت آنزیم‌های آنتی‌اکسیدانی و NO و در نتیجه افزایش سرعت مرگ‌و‌میر سلول‌ها، فیزیولوژی سلول را هدف قرار می‌دهد. | ||
| کلیدواژه‌ها | ||
| آسکوربات پراکسیداز؛ پیری؛ کاتالاز؛ گلوتاتیون ردوکتاز؛ نیتریک اکساید | ||
| عنوان مقاله [English] | ||
| Effects of seed deterioration on physiological traits, antioxidant enzymes and S-nitrosylation of proteins in two cultivars of canola (Brassica napus L.) | ||
| نویسندگان [English] | ||
| Maryam Zamanian1؛ Reza Tavakkol afshari2؛ Jafar Nabati3؛ Alireza Seifi4 | ||
| 1Department Agrotechnology/ Agriculture Faculy/ Ferdowsi university of Mashhad | ||
| 2Ferdowsi Univ. Mashhad | ||
| 3Assistant Professor of Crop Physiology, Research Center for Plant Sciences, Ferdowsi University of Mashhad | ||
| 4Assistant Professor, Department of Crop Biotechnology and Breeding, Ferdowsi University of Mashhad | ||
| چکیده [English] | ||
| This study was conducted as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications to evaluate germination rate and percentage (GP), vigor index (SV), electrical conductivity (EC), catalase (CAT), ascorbate peroxides (APX), glutathione reductase (GR) and S-nitrosothiol (SNO) at the Ferdowsi University of Mashhad, 2019-20. Accelerated Aging (AA) treatments made significant decline in germination indices and biochemical characteristics (except EC) of canola seeds. Also, Modena cultivar was stronger in germination traits while Okapi cultivar was better in biochemical activities, which can be explained by difference in the primary genetic of the cultivars. According to the results, in the treatments of 72 hours of AA compared to the control, GP has decreased by 86%, CAT 88%, APX 89% and SNOs 36%. On the top of that, there was a positive correlation between SNOs and enzymes. In conclusion, Nitric oxide (NO) is likely to indirectly affect the antioxidant enzymes by SNOs in aged canola seeds. Seed aging targets the cell physiology by reducing the activities of antioxidant enzymes and NO and therefore increasing the rate of cell mortality. | ||
| کلیدواژه‌ها [English] | ||
| Aging, Ascorbate peroxidase, Catalase, Glutathione reductase, Nitric oxide | ||
| مراجع | ||
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